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Protein

Serine/threonine-protein kinase DCLK1

Gene

DCLK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei419ATPPROSITE-ProRule annotation1
Active sitei511Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi396 – 404ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: ProtInc
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Neurogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
SignaLinkiO15075

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK1 (EC:2.7.11.1)
Alternative name(s):
Doublecortin domain-containing protein 3A
Doublecortin-like and CAM kinase-like 1
Doublecortin-like kinase 1
Gene namesi
Name:DCLK1
Synonyms:DCAMKL1, DCDC3A, KIAA0369
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000133083.14
HGNCiHGNC:2700 DCLK1
MIMi604742 gene
neXtProtiNX_O15075

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9201
OpenTargetsiENSG00000133083
PharmGKBiPA162383325

Chemistry databases

ChEMBLiCHEMBL5683

Polymorphism and mutation databases

BioMutaiDCLK1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859191 – 740Serine/threonine-protein kinase DCLK1Add BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineBy similarity1
Modified residuei36PhosphoserineBy similarity1
Modified residuei46PhosphothreonineBy similarity1
Modified residuei305PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei330PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei334PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1
Modified residuei347PhosphoserineBy similarity1
Modified residuei352PhosphoserineCombined sources1
Modified residuei353PhosphoserineBy similarity1
Modified residuei355PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei376PhosphoserineBy similarity1
Modified residuei520PhosphotyrosineBy similarity1
Modified residuei726PhosphoserineBy similarity1
Modified residuei735PhosphoserineBy similarity1
Modified residuei738PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO15075
MaxQBiO15075
PaxDbiO15075
PeptideAtlasiO15075
PRIDEiO15075
ProteomicsDBi48428
48429 [O15075-2]
48430 [O15075-3]
48431 [O15075-4]

PTM databases

CarbonylDBiO15075
iPTMnetiO15075
PhosphoSitePlusiO15075
SwissPalmiO15075

Expressioni

Tissue specificityi

In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.1 Publication

Inductioni

Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000133083 Expressed in 210 organ(s), highest expression level in parietal lobe
CleanExiHS_DCLK1
ExpressionAtlasiO15075 baseline and differential
GenevisibleiO15075 HS

Organism-specific databases

HPAiCAB012405
HPA015040
HPA015655

Interactioni

Protein-protein interaction databases

BioGridi114635, 78 interactors
IntActiO15075, 8 interactors
STRINGi9606.ENSP00000255448

Chemistry databases

BindingDBiO15075

Structurei

Secondary structure

1740
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO15075
SMRiO15075
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15075

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 143Doublecortin 1PROSITE-ProRule annotationAdd BLAST87
Domaini186 – 269Doublecortin 2PROSITE-ProRule annotationAdd BLAST84
Domaini390 – 647Protein kinasePROSITE-ProRule annotationAdd BLAST258

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi288 – 340Pro/Ser-richAdd BLAST53
Compositional biasi698 – 701Poly-Arg4

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
KOG3757 Eukaryota
ENOG410YA63 LUCA
GeneTreeiENSGT00930000150819
HOGENOMiHOG000233016
HOVERGENiHBG003790
InParanoidiO15075
KOiK08805
OMAiCASIEPY
OrthoDBiEOG091G02RF
PhylomeDBiO15075
TreeFamiTF318770

Family and domain databases

CDDicd01617 DCX, 2 hits
Gene3Di3.10.20.230, 2 hits
InterProiView protein in InterPro
IPR003533 Doublecortin_dom
IPR036572 Doublecortin_dom_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03607 DCX, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00537 DCX, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF89837 SSF89837, 2 hits
PROSITEiView protein in PROSITE
PS50309 DC, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist. Type A (AS and AL) and type B (BS and BL) isoforms differ respectively by the presence or absence of the doublecortin domain. An alternative splicing occurring in 3' of the mRNA produces the long (L) instead of the short (S) isoforms.

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 2 (identifier: O15075-1) [UniParc]FASTAAdd to basket
Also known as: AL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL
60 70 80 90 100
SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN
110 120 130 140 150
LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW
160 170 180 190 200
SVNVKTTSAS RAVSSLATAK GSPSEVRENK DFIRPKLVTI IRSGVKPRKA
210 220 230 240 250
VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG
260 270 280 290 300
DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASS SRRSTTKSPG
310 320 330 340 350
PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRSSQHG
360 370 380 390 400
GSSTSLASTK VCSSMDENDG PGEEVSEEGF QIPATITERY KVGRTIGDGN
410 420 430 440 450
FAVVKECVER STAREYALKI IKKSKCRGKE HMIQNEVSIL RRVKHPNIVL
460 470 480 490 500
LIEEMDVPTE LYLVMELVKG GDLFDAITST NKYTERDASG MLYNLASAIK
510 520 530 540 550
YLHSLNIVHR DIKPENLLVY EHQDGSKSLK LGDFGLATIV DGPLYTVCGT
560 570 580 590 600
PTYVAPEIIA ETGYGLKVDI WAAGVITYIL LCGFPPFRGS GDDQEVLFDQ
610 620 630 640 650
ILMGQVDFPS PYWDNVSDSA KELITMMLLV DVDQRFSAVQ VLEHPWVNDD
660 670 680 690 700
GLPENEHQLS VAGKIKKHFN TGPKPNSTAA GVSVIATTAL DKERQVFRRR
710 720 730 740
RNQDVRSRYK AQPAPPELNS ESEDYSPSSS ETVRSPNSPF
Length:740
Mass (Da):82,224
Last modified:May 30, 2000 - v2
Checksum:iD7B6D855099A315C
GO
Isoform 1 (identifier: O15075-2) [UniParc]FASTAAdd to basket
Also known as: AS

The sequence of this isoform differs from the canonical sequence as follows:
     687-740: TTALDKERQV...ETVRSPNSPF → LDHGFTIKRS...RFSDEDATRM

Show »
Length:729
Mass (Da):81,100
Checksum:i32C685F6B308A6BD
GO
Isoform 3 (identifier: O15075-3) [UniParc]FASTAAdd to basket
Also known as: BS

The sequence of this isoform differs from the canonical sequence as follows:
     1-307: Missing.
     308-313: PASTSS → MLELIE
     687-740: TTALDKERQV...ETVRSPNSPF → LDHGFTIKRS...RFSDEDATRM

Show »
Length:422
Mass (Da):46,557
Checksum:i945DC91D48ECDB3A
GO
Isoform 4 (identifier: O15075-4) [UniParc]FASTAAdd to basket
Also known as: BL

The sequence of this isoform differs from the canonical sequence as follows:
     1-307: Missing.
     308-313: PASTSS → MLELIE

Show »
Length:433
Mass (Da):47,681
Checksum:i7EA1B5E565ADFC9A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5VZY9Q5VZY9_HUMAN
Serine/threonine-protein kinase DCL...
DCLK1
363Annotation score:

Sequence cautioni

The sequence BAA20824 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04567329G → C in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_04567446T → M in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_04567593R → Q in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_045676291S → F in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_045677292R → H1 PublicationCorresponds to variant dbSNP:rs56185003Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0049051 – 307Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST307
Alternative sequenceiVSP_004906308 – 313PASTSS → MLELIE in isoform 3 and isoform 4. 2 Publications6
Alternative sequenceiVSP_004907687 – 740TTALD…PNSPF → LDHGFTIKRSGSLDYYQQPG MYWIRPPLLIRRGRFSDEDA TRM in isoform 1 and isoform 3. 5 PublicationsAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002367 mRNA Translation: BAA20824.2 Different initiation.
AL157760
, AL157694, AL160392, AL390029 Genomic DNA Translation: CAH70167.1
AL157760 Genomic DNA Translation: CAH70170.1
AK295777 mRNA Translation: BAH12185.1
AL157760
, AL157694, AL160392, AL390029 Genomic DNA Translation: CAH70168.1
AL160392
, AL157694, AL157760, AL390029 Genomic DNA Translation: CAH70261.1
AL160392
, AL157694, AL157760, AL390029 Genomic DNA Translation: CAH70262.1
AL157694
, AL157760, AL160392, AL390029 Genomic DNA Translation: CAH70656.1
AL157694
, AL157760, AL160392, AL390029 Genomic DNA Translation: CAH70657.1
AL390029
, AL157694, AL157760, AL160392 Genomic DNA Translation: CAI15720.1
AL390029
, AL157694, AL157760, AL160392 Genomic DNA Translation: CAI15721.1
CH471075 Genomic DNA Translation: EAX08550.1
CH471075 Genomic DNA Translation: EAX08551.1
BC152456 mRNA Translation: AAI52457.1
CCDSiCCDS55895.1 [O15075-4]
CCDS73561.1 [O15075-3]
CCDS81762.1 [O15075-1]
CCDS9354.1 [O15075-2]
RefSeqiNP_001182344.1, NM_001195415.1 [O15075-3]
NP_001182345.1, NM_001195416.1 [O15075-4]
NP_001317000.1, NM_001330071.1 [O15075-1]
NP_001317001.1, NM_001330072.1 [O15075-1]
NP_004725.1, NM_004734.4 [O15075-2]
UniGeneiHs.507755
Hs.728619

Genome annotation databases

EnsembliENST00000255448; ENSP00000255448; ENSG00000133083 [O15075-2]
ENST00000360631; ENSP00000353846; ENSG00000133083 [O15075-1]
ENST00000379893; ENSP00000369223; ENSG00000133083 [O15075-4]
ENST00000615680; ENSP00000484452; ENSG00000133083 [O15075-3]
GeneIDi9201
KEGGihsa:9201
UCSCiuc001uve.4 human [O15075-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002367 mRNA Translation: BAA20824.2 Different initiation.
AL157760
, AL157694, AL160392, AL390029 Genomic DNA Translation: CAH70167.1
AL157760 Genomic DNA Translation: CAH70170.1
AK295777 mRNA Translation: BAH12185.1
AL157760
, AL157694, AL160392, AL390029 Genomic DNA Translation: CAH70168.1
AL160392
, AL157694, AL157760, AL390029 Genomic DNA Translation: CAH70261.1
AL160392
, AL157694, AL157760, AL390029 Genomic DNA Translation: CAH70262.1
AL157694
, AL157760, AL160392, AL390029 Genomic DNA Translation: CAH70656.1
AL157694
, AL157760, AL160392, AL390029 Genomic DNA Translation: CAH70657.1
AL390029
, AL157694, AL157760, AL160392 Genomic DNA Translation: CAI15720.1
AL390029
, AL157694, AL157760, AL160392 Genomic DNA Translation: CAI15721.1
CH471075 Genomic DNA Translation: EAX08550.1
CH471075 Genomic DNA Translation: EAX08551.1
BC152456 mRNA Translation: AAI52457.1
CCDSiCCDS55895.1 [O15075-4]
CCDS73561.1 [O15075-3]
CCDS81762.1 [O15075-1]
CCDS9354.1 [O15075-2]
RefSeqiNP_001182344.1, NM_001195415.1 [O15075-3]
NP_001182345.1, NM_001195416.1 [O15075-4]
NP_001317000.1, NM_001330071.1 [O15075-1]
NP_001317001.1, NM_001330072.1 [O15075-1]
NP_004725.1, NM_004734.4 [O15075-2]
UniGeneiHs.507755
Hs.728619

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MFWX-ray1.60A49-154[»]
1MG4X-ray1.50A49-154[»]
1UF0NMR-A54-156[»]
5JZJX-ray1.71A/B372-649[»]
5JZNX-ray2.85A/B372-649[»]
ProteinModelPortaliO15075
SMRiO15075
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114635, 78 interactors
IntActiO15075, 8 interactors
STRINGi9606.ENSP00000255448

Chemistry databases

BindingDBiO15075
ChEMBLiCHEMBL5683

PTM databases

CarbonylDBiO15075
iPTMnetiO15075
PhosphoSitePlusiO15075
SwissPalmiO15075

Polymorphism and mutation databases

BioMutaiDCLK1

Proteomic databases

EPDiO15075
MaxQBiO15075
PaxDbiO15075
PeptideAtlasiO15075
PRIDEiO15075
ProteomicsDBi48428
48429 [O15075-2]
48430 [O15075-3]
48431 [O15075-4]

Protocols and materials databases

DNASUi9201
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255448; ENSP00000255448; ENSG00000133083 [O15075-2]
ENST00000360631; ENSP00000353846; ENSG00000133083 [O15075-1]
ENST00000379893; ENSP00000369223; ENSG00000133083 [O15075-4]
ENST00000615680; ENSP00000484452; ENSG00000133083 [O15075-3]
GeneIDi9201
KEGGihsa:9201
UCSCiuc001uve.4 human [O15075-1]

Organism-specific databases

CTDi9201
DisGeNETi9201
EuPathDBiHostDB:ENSG00000133083.14
GeneCardsiDCLK1
HGNCiHGNC:2700 DCLK1
HPAiCAB012405
HPA015040
HPA015655
MIMi604742 gene
neXtProtiNX_O15075
OpenTargetsiENSG00000133083
PharmGKBiPA162383325
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
KOG3757 Eukaryota
ENOG410YA63 LUCA
GeneTreeiENSGT00930000150819
HOGENOMiHOG000233016
HOVERGENiHBG003790
InParanoidiO15075
KOiK08805
OMAiCASIEPY
OrthoDBiEOG091G02RF
PhylomeDBiO15075
TreeFamiTF318770

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
SignaLinkiO15075

Miscellaneous databases

ChiTaRSiDCLK1 human
EvolutionaryTraceiO15075
GeneWikiiDCLK1
GenomeRNAii9201
PROiPR:O15075
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133083 Expressed in 210 organ(s), highest expression level in parietal lobe
CleanExiHS_DCLK1
ExpressionAtlasiO15075 baseline and differential
GenevisibleiO15075 HS

Family and domain databases

CDDicd01617 DCX, 2 hits
Gene3Di3.10.20.230, 2 hits
InterProiView protein in InterPro
IPR003533 Doublecortin_dom
IPR036572 Doublecortin_dom_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03607 DCX, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00537 DCX, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF89837 SSF89837, 2 hits
PROSITEiView protein in PROSITE
PS50309 DC, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCLK1_HUMAN
AccessioniPrimary (citable) accession number: O15075
Secondary accession number(s): B7Z3E9
, Q5VZY8, Q5VZZ0, Q5VZZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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