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UniProtKB - O15075 (DCLK1_HUMAN)
Protein
Serine/threonine-protein kinase DCLK1
Gene
DCLK1
Organism
Homo sapiens (Human)
Status
Functioni
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 419 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 511 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 396 – 404 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: ProtInc
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
GO - Biological processi
- axon extension Source: Ensembl
- central nervous system development Source: ProtInc
- central nervous system projection neuron axonogenesis Source: Ensembl
- dendrite morphogenesis Source: Ensembl
- endosomal transport Source: UniProtKB
- forebrain development Source: Ensembl
- intracellular signal transduction Source: InterPro
- negative regulation of protein localization to nucleus Source: Ensembl
- nervous system development Source: ProtInc
- neuron migration Source: Ensembl
- neuron projection morphogenesis Source: GO_Central
- peptidyl-serine phosphorylation Source: Ensembl
- protein phosphorylation Source: ProtInc
- response to virus Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation, Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 2681 |
PathwayCommonsi | O15075 |
SignaLinki | O15075 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase DCLK1 (EC:2.7.11.1)Alternative name(s): Doublecortin domain-containing protein 3A Doublecortin-like and CAM kinase-like 1 Doublecortin-like kinase 1 |
Gene namesi | Name:DCLK1 Synonyms:DCAMKL1, DCDC3A, KIAA0369 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2700, DCLK1 |
MIMi | 604742, gene |
neXtProti | NX_O15075 |
VEuPathDBi | HostDB:ENSG00000133083 |
Subcellular locationi
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
Other locations
- postsynaptic density Source: Ensembl
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 9201 |
OpenTargetsi | ENSG00000133083 |
PharmGKBi | PA162383325 |
Miscellaneous databases
Pharosi | O15075, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5683 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | O15075 |
GuidetoPHARMACOLOGYi | 2005 |
Genetic variation databases
BioMutai | DCLK1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085919 | 1 – 740 | Serine/threonine-protein kinase DCLK1Add BLAST | 740 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 32 | PhosphoserineBy similarity | 1 | |
Modified residuei | 36 | PhosphoserineBy similarity | 1 | |
Modified residuei | 46 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 305 | PhosphoserineBy similarity | 1 | |
Modified residuei | 307 | PhosphoserineBy similarity | 1 | |
Modified residuei | 330 | PhosphoserineBy similarity | 1 | |
Modified residuei | 332 | PhosphoserineBy similarity | 1 | |
Modified residuei | 334 | PhosphoserineBy similarity | 1 | |
Modified residuei | 337 | PhosphoserineBy similarity | 1 | |
Modified residuei | 347 | PhosphoserineBy similarity | 1 | |
Modified residuei | 352 | PhosphoserineCombined sources | 1 | |
Modified residuei | 353 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineBy similarity | 1 | |
Modified residuei | 364 | PhosphoserineBy similarity | 1 | |
Modified residuei | 376 | PhosphoserineBy similarity | 1 | |
Modified residuei | 520 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 726 | PhosphoserineBy similarity | 1 | |
Modified residuei | 735 | PhosphoserineBy similarity | 1 | |
Modified residuei | 738 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O15075 |
jPOSTi | O15075 |
MassIVEi | O15075 |
MaxQBi | O15075 |
PaxDbi | O15075 |
PeptideAtlasi | O15075 |
PRIDEi | O15075 |
ProteomicsDBi | 48428 [O15075-1] 48429 [O15075-2] 48430 [O15075-3] 48431 [O15075-4] |
PTM databases
CarbonylDBi | O15075 |
iPTMneti | O15075 |
PhosphoSitePlusi | O15075 |
SwissPalmi | O15075 |
Expressioni
Tissue specificityi
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.1 Publication
Inductioni
Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000133083, Expressed in parietal lobe and 222 other tissues |
ExpressionAtlasi | O15075, baseline and differential |
Genevisiblei | O15075, HS |
Organism-specific databases
HPAi | ENSG00000133083, Tissue enhanced (brain) |
Interactioni
Binary interactionsi
Isoform 1 [O15075-2]
With | #Exp. | IntAct |
---|---|---|
CDC23 [Q9UJX2] | 3 | EBI-12324841,EBI-396137 |
EFHC2 [Q5JST6] | 3 | EBI-12324841,EBI-2349927 |
Protein-protein interaction databases
BioGRIDi | 114635, 91 interactors |
IntActi | O15075, 19 interactors |
MINTi | O15075 |
STRINGi | 9606.ENSP00000255448 |
Chemistry databases
BindingDBi | O15075 |
Miscellaneous databases
RNActi | O15075, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O15075 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O15075 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 143 | Doublecortin 1PROSITE-ProRule annotationAdd BLAST | 87 | |
Domaini | 186 – 269 | Doublecortin 2PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 390 – 647 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 258 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 287 – 378 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 697 – 740 | DisorderedSequence analysisAdd BLAST | 44 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 287 – 365 | Polar residuesSequence analysisAdd BLAST | 79 | |
Compositional biasi | 719 – 740 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0032, Eukaryota KOG3757, Eukaryota |
GeneTreei | ENSGT00940000154956 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | O15075 |
OMAi | WRLTLAN |
OrthoDBi | 330091at2759 |
PhylomeDBi | O15075 |
TreeFami | TF318770 |
Family and domain databases
Gene3Di | 3.10.20.230, 2 hits |
InterProi | View protein in InterPro IPR003533, Doublecortin_dom IPR036572, Doublecortin_dom_sf IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03607, DCX, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00537, DCX, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit SSF89837, SSF89837, 2 hits |
PROSITEi | View protein in PROSITE PS50309, DC, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist. Type A (AS and AL) and type B (BS and BL) isoforms differ respectively by the presence or absence of the doublecortin domain. An alternative splicing occurring in 3' of the mRNA produces the long (L) instead of the short (S) isoforms.
This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 2 (identifier: O15075-1) [UniParc]FASTAAdd to basket
Also known as: AL
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL
60 70 80 90 100
SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN
110 120 130 140 150
LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW
160 170 180 190 200
SVNVKTTSAS RAVSSLATAK GSPSEVRENK DFIRPKLVTI IRSGVKPRKA
210 220 230 240 250
VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG
260 270 280 290 300
DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASS SRRSTTKSPG
310 320 330 340 350
PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRSSQHG
360 370 380 390 400
GSSTSLASTK VCSSMDENDG PGEEVSEEGF QIPATITERY KVGRTIGDGN
410 420 430 440 450
FAVVKECVER STAREYALKI IKKSKCRGKE HMIQNEVSIL RRVKHPNIVL
460 470 480 490 500
LIEEMDVPTE LYLVMELVKG GDLFDAITST NKYTERDASG MLYNLASAIK
510 520 530 540 550
YLHSLNIVHR DIKPENLLVY EHQDGSKSLK LGDFGLATIV DGPLYTVCGT
560 570 580 590 600
PTYVAPEIIA ETGYGLKVDI WAAGVITYIL LCGFPPFRGS GDDQEVLFDQ
610 620 630 640 650
ILMGQVDFPS PYWDNVSDSA KELITMMLLV DVDQRFSAVQ VLEHPWVNDD
660 670 680 690 700
GLPENEHQLS VAGKIKKHFN TGPKPNSTAA GVSVIATTAL DKERQVFRRR
710 720 730 740
RNQDVRSRYK AQPAPPELNS ESEDYSPSSS ETVRSPNSPF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5VZY9 | Q5VZY9_HUMAN | Serine/threonine-protein kinase DCL... | DCLK1 | 363 | Annotation score: |
Sequence cautioni
The sequence BAA20824 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_045673 | 29 | G → C in a gastric adenocarcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_045674 | 46 | T → M in a gastric adenocarcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_045675 | 93 | R → Q in a gastric adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1366698690Ensembl. | 1 | |
Natural variantiVAR_045676 | 291 | S → F in a gastric adenocarcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_045677 | 292 | R → H1 PublicationCorresponds to variant dbSNP:rs56185003Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004905 | 1 – 307 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 307 | |
Alternative sequenceiVSP_004906 | 308 – 313 | PASTSS → MLELIE in isoform 3 and isoform 4. 2 Publications | 6 | |
Alternative sequenceiVSP_004907 | 687 – 740 | TTALD…PNSPF → LDHGFTIKRSGSLDYYQQPG MYWIRPPLLIRRGRFSDEDA TRM in isoform 1 and isoform 3. 5 PublicationsAdd BLAST | 54 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000255448; ENSP00000255448; ENSG00000133083 [O15075-2] ENST00000360631; ENSP00000353846; ENSG00000133083 ENST00000379893; ENSP00000369223; ENSG00000133083 [O15075-4] ENST00000615680; ENSP00000484452; ENSG00000133083 [O15075-3] |
GeneIDi | 9201 |
KEGGi | hsa:9201 |
MANE-Selecti | ENST00000360631.8; ENSP00000353846.3; NM_001330071.2; NP_001317000.1 |
UCSCi | uc001uve.4, human [O15075-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MFW | X-ray | 1.60 | A | 49-154 | [»] | |
1MG4 | X-ray | 1.50 | A | 49-154 | [»] | |
1UF0 | NMR | - | A | 54-156 | [»] | |
5JZJ | X-ray | 1.71 | A/B | 372-649 | [»] | |
5JZN | X-ray | 2.85 | A/B | 372-649 | [»] | |
6KYQ | X-ray | 2.14 | A/B | 379-704 | [»] | |
6KYR | X-ray | 2.21 | A/B | 379-704 | [»] | |
7F3G | X-ray | 2.10 | A/B | 372-649 | [»] | |
7KX6 | X-ray | 2.50 | A/B | 372-649 | [»] | |
7KX8 | X-ray | 3.10 | A/B | 372-686 | [»] | |
7KXW | X-ray | 3.00 | A/B | 372-649 | [»] | |
SMRi | O15075 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114635, 91 interactors |
IntActi | O15075, 19 interactors |
MINTi | O15075 |
STRINGi | 9606.ENSP00000255448 |
Chemistry databases
BindingDBi | O15075 |
ChEMBLi | CHEMBL5683 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | O15075 |
GuidetoPHARMACOLOGYi | 2005 |
PTM databases
CarbonylDBi | O15075 |
iPTMneti | O15075 |
PhosphoSitePlusi | O15075 |
SwissPalmi | O15075 |
Genetic variation databases
BioMutai | DCLK1 |
Proteomic databases
EPDi | O15075 |
jPOSTi | O15075 |
MassIVEi | O15075 |
MaxQBi | O15075 |
PaxDbi | O15075 |
PeptideAtlasi | O15075 |
PRIDEi | O15075 |
ProteomicsDBi | 48428 [O15075-1] 48429 [O15075-2] 48430 [O15075-3] 48431 [O15075-4] |
Protocols and materials databases
Antibodypediai | 23046, 435 antibodies from 39 providers |
DNASUi | 9201 |
Genome annotation databases
Ensembli | ENST00000255448; ENSP00000255448; ENSG00000133083 [O15075-2] ENST00000360631; ENSP00000353846; ENSG00000133083 ENST00000379893; ENSP00000369223; ENSG00000133083 [O15075-4] ENST00000615680; ENSP00000484452; ENSG00000133083 [O15075-3] |
GeneIDi | 9201 |
KEGGi | hsa:9201 |
MANE-Selecti | ENST00000360631.8; ENSP00000353846.3; NM_001330071.2; NP_001317000.1 |
UCSCi | uc001uve.4, human [O15075-1] |
Organism-specific databases
CTDi | 9201 |
DisGeNETi | 9201 |
GeneCardsi | DCLK1 |
HGNCi | HGNC:2700, DCLK1 |
HPAi | ENSG00000133083, Tissue enhanced (brain) |
MIMi | 604742, gene |
neXtProti | NX_O15075 |
OpenTargetsi | ENSG00000133083 |
PharmGKBi | PA162383325 |
VEuPathDBi | HostDB:ENSG00000133083 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota KOG3757, Eukaryota |
GeneTreei | ENSGT00940000154956 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | O15075 |
OMAi | WRLTLAN |
OrthoDBi | 330091at2759 |
PhylomeDBi | O15075 |
TreeFami | TF318770 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 2681 |
PathwayCommonsi | O15075 |
SignaLinki | O15075 |
Miscellaneous databases
BioGRID-ORCSi | 9201, 7 hits in 1069 CRISPR screens |
ChiTaRSi | DCLK1, human |
EvolutionaryTracei | O15075 |
GeneWikii | DCLK1 |
GenomeRNAii | 9201 |
Pharosi | O15075, Tchem |
PROi | PR:O15075 |
RNActi | O15075, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000133083, Expressed in parietal lobe and 222 other tissues |
ExpressionAtlasi | O15075, baseline and differential |
Genevisiblei | O15075, HS |
Family and domain databases
Gene3Di | 3.10.20.230, 2 hits |
InterProi | View protein in InterPro IPR003533, Doublecortin_dom IPR036572, Doublecortin_dom_sf IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03607, DCX, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00537, DCX, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit SSF89837, SSF89837, 2 hits |
PROSITEi | View protein in PROSITE PS50309, DC, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCLK1_HUMAN | |
Accessioni | O15075Primary (citable) accession number: O15075 Secondary accession number(s): B7Z3E9 Q5VZZ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families