UniProtKB - O14967 (CLGN_HUMAN)
Protein
Calmegin
Gene
CLGN
Organism
Homo sapiens (Human)
Status
Functioni
Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).By similarity
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- protein folding chaperone Source: Ensembl
- unfolded protein binding Source: ProtInc
GO - Biological processi
- binding of sperm to zona pellucida Source: Ensembl
- endoplasmic reticulum unfolded protein response Source: GO_Central
- protein-containing complex assembly Source: Ensembl
- protein folding Source: GO_Central
- single fertilization Source: ProtInc
Keywordsi
Molecular function | Chaperone |
Ligand | Calcium |
Enzyme and pathway databases
PathwayCommonsi | O14967 |
Names & Taxonomyi
Protein namesi | Recommended name: Calmegin |
Gene namesi | Name:CLGN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2060, CLGN |
MIMi | 601858, gene |
neXtProti | NX_O14967 |
VEuPathDBi | HostDB:ENSG00000153132.12 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: HPA
- endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear envelope Source: Ensembl
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 471 | LumenalSequence analysisAdd BLAST | 452 | |
Transmembranei | 472 – 492 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 493 – 610 | CytoplasmicSequence analysisAdd BLAST | 118 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 1047 |
OpenTargetsi | ENSG00000153132 |
PharmGKBi | PA26587 |
Miscellaneous databases
Pharosi | O14967, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295653 |
Genetic variation databases
BioMutai | CLGN |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000004210 | 20 – 610 | CalmeginAdd BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 128 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 151 ↔ 185 | By similarity | ||
Disulfide bondi | 351 ↔ 355 | By similarity | ||
Modified residuei | 560 | PhosphoserineCombined sources | 1 | |
Modified residuei | 576 | PhosphoserineCombined sources | 1 | |
Modified residuei | 579 | PhosphoserineBy similarity | 1 | |
Modified residuei | 581 | PhosphoserineBy similarity | 1 | |
Modified residuei | 591 | PhosphoserineBy similarity | 1 | |
Modified residuei | 594 | PhosphoserineBy similarity | 1 | |
Modified residuei | 601 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinProteomic databases
EPDi | O14967 |
jPOSTi | O14967 |
MassIVEi | O14967 |
MaxQBi | O14967 |
PaxDbi | O14967 |
PeptideAtlasi | O14967 |
PRIDEi | O14967 |
ProteomicsDBi | 48342 [O14967-1] |
PTM databases
iPTMneti | O14967 |
PhosphoSitePlusi | O14967 |
SwissPalmi | O14967 |
Expressioni
Tissue specificityi
Detected in testis (at protein level). Detected in testis.1 Publication
Gene expression databases
Bgeei | ENSG00000153132, Expressed in testis and 165 other tissues |
ExpressionAtlasi | O14967, baseline and differential |
Genevisiblei | O14967, HS |
Organism-specific databases
HPAi | ENSG00000153132, Tissue enhanced (heart muscle, pituitary gland, testis) |
Interactioni
Subunit structurei
Interacts with PPIB.
Interacts with ADAM2 (By similarity).
Interacts with PDILT.
By similarity1 PublicationGO - Molecular functioni
- unfolded protein binding Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 107477, 62 interactors |
IntActi | O14967, 20 interactors |
STRINGi | 9606.ENSP00000326699 |
Miscellaneous databases
RNActi | O14967, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 267 – 280 | 1-1Add BLAST | 14 | |
Repeati | 284 – 297 | 1-2Add BLAST | 14 | |
Repeati | 303 – 316 | 1-3Add BLAST | 14 | |
Repeati | 322 – 335 | 1-4Add BLAST | 14 | |
Repeati | 339 – 352 | 2-1Add BLAST | 14 | |
Repeati | 356 – 369 | 2-2Add BLAST | 14 | |
Repeati | 370 – 383 | 2-3Add BLAST | 14 | |
Repeati | 384 – 397 | 2-4Add BLAST | 14 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 317 – 350 | Interaction with PPIBBy similarityAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0675, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_2_0_1 |
InParanoidi | O14967 |
OMAi | EMFIDQS |
PhylomeDBi | O14967 |
TreeFami | TF300618 |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O14967-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHFQAFWLCL GLLFISINAE FMDDDVETED FEENSEEIDV NESELSSEIK
60 70 80 90 100
YKTPQPIGEV YFAETFDSGR LAGWVLSKAK KDDMDEEISI YDGRWEIEEL
110 120 130 140 150
KENQVPGDRG LVLKSRAKHH AISAVLAKPF IFADKPLIVQ YEVNFQDGID
160 170 180 190 200
CGGAYIKLLA DTDDLILENF YDKTSYIIMF GPDKCGEDYK LHFIFRHKHP
210 220 230 240 250
KTGVFEEKHA KPPDVDLKKF FTDRKTHLYT LVMNPDDTFE VLVDQTVVNK
260 270 280 290 300
GSLLEDVVPP IKPPKEIEDP NDKKPEEWDE RAKIPDPSAV KPEDWDESEP
310 320 330 340 350
AQIEDSSVVK PAGWLDDEPK FIPDPNAEKP DDWNEDTDGE WEAPQILNPA
360 370 380 390 400
CRIGCGEWKP PMIDNPKYKG VWRPPLVDNP NYQGIWSPRK IPNPDYFEDD
410 420 430 440 450
HPFLLTSFSA LGLELWSMTS DIYFDNFIIC SEKEVADHWA ADGWRWKIMI
460 470 480 490 500
ANANKPGVLK QLMAAAEGHP WLWLIYLVTA GVPIALITSF CWPRKVKKKH
510 520 530 540 550
KDTEYKKTDI CIPQTKGVLE QEEKEEKAAL EKPMDLEEEK KQNDGEMLEK
560 570 580 590 600
EEESEPEEKS EEEIEIIEGQ EESNQSNKSG SEDEMKEADE STGSGDGPIK
610
SVRKRRVRKD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RAZ4 | D6RAZ4_HUMAN | Calmegin | CLGN | 179 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 232 | V → A in BAG63520 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024400 | 160 | A → S. Corresponds to variant dbSNP:rs2567241Ensembl. | 1 | |
Natural variantiVAR_033776 | 290 | V → I. Corresponds to variant dbSNP:rs2175563Ensembl. | 1 | |
Natural variantiVAR_048590 | 352 | R → W. Corresponds to variant dbSNP:rs12513290Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055517 | 54 – 211 | Missing in isoform 2. 1 PublicationAdd BLAST | 158 | |
Alternative sequenceiVSP_055518 | 378 – 424 | Missing in isoform 2. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86322 mRNA Translation: BAA22590.1 AK093096 mRNA Translation: BAG52652.1 AK302149 mRNA Translation: BAG63520.1 CH471056 Genomic DNA Translation: EAX05099.1 CH471056 Genomic DNA Translation: EAX05100.1 CH471056 Genomic DNA Translation: EAX05101.1 BC028357 mRNA Translation: AAH28357.1 |
CCDSi | CCDS3751.1 [O14967-1] |
RefSeqi | NP_001124147.1, NM_001130675.1 [O14967-1] NP_004353.1, NM_004362.2 [O14967-1] |
Genome annotation databases
Ensembli | ENST00000325617; ENSP00000326699; ENSG00000153132 [O14967-1] ENST00000414773; ENSP00000392782; ENSG00000153132 [O14967-1] |
GeneIDi | 1047 |
KEGGi | hsa:1047 |
UCSCi | uc003iii.4, human [O14967-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86322 mRNA Translation: BAA22590.1 AK093096 mRNA Translation: BAG52652.1 AK302149 mRNA Translation: BAG63520.1 CH471056 Genomic DNA Translation: EAX05099.1 CH471056 Genomic DNA Translation: EAX05100.1 CH471056 Genomic DNA Translation: EAX05101.1 BC028357 mRNA Translation: AAH28357.1 |
CCDSi | CCDS3751.1 [O14967-1] |
RefSeqi | NP_001124147.1, NM_001130675.1 [O14967-1] NP_004353.1, NM_004362.2 [O14967-1] |
3D structure databases
SMRi | O14967 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 107477, 62 interactors |
IntActi | O14967, 20 interactors |
STRINGi | 9606.ENSP00000326699 |
Chemistry databases
ChEMBLi | CHEMBL4295653 |
PTM databases
iPTMneti | O14967 |
PhosphoSitePlusi | O14967 |
SwissPalmi | O14967 |
Genetic variation databases
BioMutai | CLGN |
Proteomic databases
EPDi | O14967 |
jPOSTi | O14967 |
MassIVEi | O14967 |
MaxQBi | O14967 |
PaxDbi | O14967 |
PeptideAtlasi | O14967 |
PRIDEi | O14967 |
ProteomicsDBi | 48342 [O14967-1] |
Protocols and materials databases
Antibodypediai | 16228, 175 antibodies |
DNASUi | 1047 |
Genome annotation databases
Ensembli | ENST00000325617; ENSP00000326699; ENSG00000153132 [O14967-1] ENST00000414773; ENSP00000392782; ENSG00000153132 [O14967-1] |
GeneIDi | 1047 |
KEGGi | hsa:1047 |
UCSCi | uc003iii.4, human [O14967-1] |
Organism-specific databases
CTDi | 1047 |
DisGeNETi | 1047 |
GeneCardsi | CLGN |
HGNCi | HGNC:2060, CLGN |
HPAi | ENSG00000153132, Tissue enhanced (heart muscle, pituitary gland, testis) |
MIMi | 601858, gene |
neXtProti | NX_O14967 |
OpenTargetsi | ENSG00000153132 |
PharmGKBi | PA26587 |
VEuPathDBi | HostDB:ENSG00000153132.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0675, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_2_0_1 |
InParanoidi | O14967 |
OMAi | EMFIDQS |
PhylomeDBi | O14967 |
TreeFami | TF300618 |
Enzyme and pathway databases
PathwayCommonsi | O14967 |
Miscellaneous databases
BioGRID-ORCSi | 1047, 8 hits in 881 CRISPR screens |
ChiTaRSi | CLGN, human |
GeneWikii | Calmegin |
GenomeRNAii | 1047 |
Pharosi | O14967, Tbio |
PROi | PR:O14967 |
RNActi | O14967, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000153132, Expressed in testis and 165 other tissues |
ExpressionAtlasi | O14967, baseline and differential |
Genevisiblei | O14967, HS |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLGN_HUMAN | |
Accessioni | O14967Primary (citable) accession number: O14967 Secondary accession number(s): B3KS90, B4DXV8, D3DNY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 195 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants