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Protein

Myosin regulatory light chain 12B

Gene

MYL12B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.1 Publication

Miscellaneous

This chain binds calcium.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi42 – 53PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMotor protein, Muscle protein, Myosin
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-3928663 EPHA-mediated growth cone collapse
R-HSA-416572 Sema4D induced cell migration and growth-cone collapse
R-HSA-445355 Smooth Muscle Contraction
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625900 RHO GTPases activate CIT
R-HSA-5627117 RHO GTPases Activate ROCKs
R-HSA-5627123 RHO GTPases activate PAKs
SIGNORiO14950

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 12B
Alternative name(s):
MLC-2A
Short name:
MLC-2
Myosin regulatory light chain 2-B, smooth muscle isoform
Myosin regulatory light chain 20 kDa
Short name:
MLC20
Myosin regulatory light chain MRLC2
SHUJUN-1
Gene namesi
Name:MYL12B
Synonyms:MRLC2, MYLC2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000118680.12
HGNCiHGNC:29827 MYL12B
neXtProtiNX_O14950

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19 – 20TS → AA: Shows a decrease in the number of actin filament bundles. 3 Publications2
Mutagenesisi19 – 20TS → DD: Shows a larger number of actin filament bundles. 3 Publications2

Organism-specific databases

DisGeNETi103910
10627
OpenTargetsiENSG00000118680
PharmGKBiPA164723274

Polymorphism and mutation databases

BioMutaiMYL12B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003493641 – 172Myosin regulatory light chain 12BAdd BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphothreonine; by MLCK and ZIPK/DAPK33 Publications1
Modified residuei20Phosphoserine; by MLCK and ZIPK/DAPK33 Publications1

Post-translational modificationi

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14950
MaxQBiO14950
PaxDbiO14950
PeptideAtlasiO14950
PRIDEiO14950
ProteomicsDBi48333
TopDownProteomicsiO14950

PTM databases

iPTMnetiO14950
PhosphoSitePlusiO14950
SwissPalmiO14950

Expressioni

Tissue specificityi

Ubiquitously expressed in various hematopoietic cells.2 Publications

Gene expression databases

BgeeiENSG00000118680 Expressed in 224 organ(s), highest expression level in colonic mucosa
GenevisibleiO14950 HS

Organism-specific databases

HPAiHPA039262
HPA045244
HPA059197

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi125182, 41 interactors
IntActiO14950, 23 interactors
MINTiO14950
STRINGi9606.ENSP00000237500

Structurei

3D structure databases

ProteinModelPortaliO14950
SMRiO14950
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 64EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini98 – 133EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini134 – 169EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000119196
HOGENOMiHOG000233018
InParanoidiO14950
KOiK12757
OMAiDEEGTGM
OrthoDBiEOG091G0QF6
PhylomeDBiO14950
TreeFamiTF314218

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR015070 EF_hand_DJBP
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF08976 EF-hand_11, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

O14950-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID
60 70 80 90 100
KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE
110 120 130 140 150
DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK
160 170
KGNFNYIEFT RILKHGAKDK DD
Length:172
Mass (Da):19,779
Last modified:September 2, 2008 - v2
Checksum:i78FF911630F3870B
GO

Sequence cautioni

The sequence AAP73808 differs from that shown. Reason: Frameshift at position 4.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72A → V in AAP73808 (Ref. 5) Curated1
Sequence conflicti89M → T in AAP73808 (Ref. 5) Curated1
Sequence conflicti104R → G in AAP73808 (Ref. 5) Curated1
Sequence conflicti138E → G in BAA23323 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046371141E → G. Corresponds to variant dbSNP:rs14720Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82058 mRNA Translation: BAB88918.1
AB046614 mRNA Translation: BAB62403.1
D50372 mRNA Translation: BAA23323.1
U26162 mRNA Translation: AAA67367.1
AY320408 mRNA Translation: AAP73808.1 Frameshift.
AK291726 mRNA Translation: BAF84415.1
CH471113 Genomic DNA Translation: EAX01675.1
CH471113 Genomic DNA Translation: EAX01676.1
CH471113 Genomic DNA Translation: EAX01677.1
BC004994 mRNA Translation: AAH04994.1
CCDSiCCDS11831.1
RefSeqiNP_001138416.1, NM_001144944.1
NP_001138417.1, NM_001144945.1
NP_001289978.1, NM_001303049.1
NP_291024.1, NM_033546.3
UniGeneiHs.190086
Hs.464472

Genome annotation databases

EnsembliENST00000237500; ENSP00000237500; ENSG00000118680
ENST00000400175; ENSP00000383037; ENSG00000118680
ENST00000581193; ENSP00000463559; ENSG00000118680
ENST00000584539; ENSP00000464464; ENSG00000118680
GeneIDi103910
10627
KEGGihsa:103910
UCSCiuc002klt.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82058 mRNA Translation: BAB88918.1
AB046614 mRNA Translation: BAB62403.1
D50372 mRNA Translation: BAA23323.1
U26162 mRNA Translation: AAA67367.1
AY320408 mRNA Translation: AAP73808.1 Frameshift.
AK291726 mRNA Translation: BAF84415.1
CH471113 Genomic DNA Translation: EAX01675.1
CH471113 Genomic DNA Translation: EAX01676.1
CH471113 Genomic DNA Translation: EAX01677.1
BC004994 mRNA Translation: AAH04994.1
CCDSiCCDS11831.1
RefSeqiNP_001138416.1, NM_001144944.1
NP_001138417.1, NM_001144945.1
NP_001289978.1, NM_001303049.1
NP_291024.1, NM_033546.3
UniGeneiHs.190086
Hs.464472

3D structure databases

ProteinModelPortaliO14950
SMRiO14950
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125182, 41 interactors
IntActiO14950, 23 interactors
MINTiO14950
STRINGi9606.ENSP00000237500

PTM databases

iPTMnetiO14950
PhosphoSitePlusiO14950
SwissPalmiO14950

Polymorphism and mutation databases

BioMutaiMYL12B

Proteomic databases

EPDiO14950
MaxQBiO14950
PaxDbiO14950
PeptideAtlasiO14950
PRIDEiO14950
ProteomicsDBi48333
TopDownProteomicsiO14950

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237500; ENSP00000237500; ENSG00000118680
ENST00000400175; ENSP00000383037; ENSG00000118680
ENST00000581193; ENSP00000463559; ENSG00000118680
ENST00000584539; ENSP00000464464; ENSG00000118680
GeneIDi103910
10627
KEGGihsa:103910
UCSCiuc002klt.5 human

Organism-specific databases

CTDi103910
10627
DisGeNETi103910
10627
EuPathDBiHostDB:ENSG00000118680.12
GeneCardsiMYL12B
H-InvDBiHIX0014302
HGNCiHGNC:29827 MYL12B
HPAiHPA039262
HPA045244
HPA059197
neXtProtiNX_O14950
OpenTargetsiENSG00000118680
PharmGKBiPA164723274
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0031 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000119196
HOGENOMiHOG000233018
InParanoidiO14950
KOiK12757
OMAiDEEGTGM
OrthoDBiEOG091G0QF6
PhylomeDBiO14950
TreeFamiTF314218

Enzyme and pathway databases

ReactomeiR-HSA-3928663 EPHA-mediated growth cone collapse
R-HSA-416572 Sema4D induced cell migration and growth-cone collapse
R-HSA-445355 Smooth Muscle Contraction
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625900 RHO GTPases activate CIT
R-HSA-5627117 RHO GTPases Activate ROCKs
R-HSA-5627123 RHO GTPases activate PAKs
SIGNORiO14950

Miscellaneous databases

ChiTaRSiMYL12B human
PROiPR:O14950

Gene expression databases

BgeeiENSG00000118680 Expressed in 224 organ(s), highest expression level in colonic mucosa
GenevisibleiO14950 HS

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR015070 EF_hand_DJBP
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF08976 EF-hand_11, 1 hit
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiML12B_HUMAN
AccessioniPrimary (citable) accession number: O14950
Secondary accession number(s): D3DUH6, Q13182, Q7Z5Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: September 12, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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