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Protein

Calpain-9

Gene

CAPN9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81Calcium 1; via carbonyl oxygen1
Metal bindingi83Calcium 1; via carbonyl oxygen1
Metal bindingi88Calcium 11
Active sitei971 Publication1
Metal bindingi167Calcium 11
Active sitei2541 Publication1
Active sitei2781 Publication1
Metal bindingi284Calcium 21
Metal bindingi291Calcium 21
Metal bindingi312Calcium 2; via carbonyl oxygen1
Metal bindingi314Calcium 21
Metal bindingi316Calcium 2; via carbonyl oxygen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi574 – 5851PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi604 – 6152PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  • calcium ion binding Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B29 2681
ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.006

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-9 (EC:3.4.22.-)
Alternative name(s):
Digestive tract-specific calpain
New calpain 4
Short name:
nCL-4
Protein CG36
Gene namesi
Name:CAPN9
Synonyms:NCL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000135773.12
HGNCiHGNC:1486 CAPN9
MIMi606401 gene
neXtProtiNX_O14815

Subcellular locationi

Pathology & Biotechi

Organism-specific databases

DisGeNETi10753
OpenTargetsiENSG00000135773
PharmGKBiPA26066

Polymorphism and mutation databases

BioMutaiCAPN9

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077221 – 690Calpain-9Add BLAST690

Proteomic databases

EPDiO14815
PaxDbiO14815
PeptideAtlasiO14815
PRIDEiO14815
ProteomicsDBi48253
48254 [O14815-2]

PTM databases

iPTMnetiO14815
PhosphoSitePlusiO14815

Expressioni

Tissue specificityi

Expressed predominantly in stomach.

Inductioni

Down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.

Gene expression databases

BgeeiENSG00000135773 Expressed in 104 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_CAPN9
ExpressionAtlasiO14815 baseline and differential
GenevisibleiO14815 HS

Organism-specific databases

HPAiCAB033483
HPA020398

Interactioni

Protein-protein interaction databases

BioGridi115976, 2 interactors
STRINGi9606.ENSP00000271971

Structurei

Secondary structure

1690
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO14815
SMRiO14815
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14815

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 337Calpain catalyticPROSITE-ProRule annotationAdd BLAST296
Domaini518 – 552EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini561 – 594EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini591 – 626EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni338 – 521Domain IIIAdd BLAST184
Regioni522 – 690Domain IVAdd BLAST169

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiO14815
KOiK08578
OMAiINLTMNI
OrthoDBiEOG091G049E
PhylomeDBiO14815
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029542 CAPN9
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF385 PTHR10183:SF385, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O14815-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPYLYRAPGP QAHPVPKDAR ITHSSGQSFE QMRQECLQRG TLFEDADFPA
60 70 80 90 100
SNSSLFYSER PQIPFVWKRP GEIVKNPEFI LGGATRTDIC QGELGDCWLL
110 120 130 140 150
AAIASLTLNQ KALARVIPQD QSFGPGYAGI FHFQFWQHSE WLDVVIDDRL
160 170 180 190 200
PTFRDRLVFL HSADHNEFWS ALLEKAYAKL NGSYEALKGG SAIEAMEDFT
210 220 230 240 250
GGVAETFQTK EAPENFYEIL EKALKRGSLL GCFIDTRSAA ESEARTPFGL
260 270 280 290 300
IKGHAYSVTG IDQVSFRGQR IELIRIRNPW GQVEWNGSWS DSSPEWRSVG
310 320 330 340 350
PAEQKRLCHT ALDDGEFWMA FKDFKAHFDK VEICNLTPDA LEEDAIHKWE
360 370 380 390 400
VTVHQGSWVR GSTAGGCRNF LDTFWTNPQI KLSLTEKDEG QEECSFLVAL
410 420 430 440 450
MQKDRRKLKR FGANVLTIGY AIYECPDKDE HLNKDFFRYH ASRARSKTFI
460 470 480 490 500
NLREVSDRFK LPPGEYILIP STFEPHQEAD FCLRIFSEKK AITRDMDGNV
510 520 530 540 550
DIDLPEPPKP TPPDQETEEE QRFRALFEQV AGEDMEVTAE ELEYVLNAVL
560 570 580 590 600
QKKKDIKFKK LSLISCKNII SLMDTSGNGK LEFDEFKVFW DKLKQWINLF
610 620 630 640 650
LRFDADKSGT MSTYELRTAL KAAGFQLSSH LLQLIVLRYA DEELQLDFDD
660 670 680 690
FLNCLVRLEN ASRVFQALST KNKEFIHLNI NEFIHLTMNI
Length:690
Mass (Da):79,097
Last modified:January 1, 1998 - v1
Checksum:i02176C04BE1E5C23
GO
Isoform 2 (identifier: O14815-2) [UniParc]FASTAAdd to basket
Also known as: CG36

The sequence of this isoform differs from the canonical sequence as follows:
     292-318: SSPEWRSVGPAEQKRLCHTALDDGEFW → R

Show »
Length:664
Mass (Da):76,168
Checksum:i8D0524E4A476159D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7ESS6E7ESS6_HUMAN
Calpain-9
CAPN9
627Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022188102A → V1 PublicationCorresponds to variant dbSNP:rs12562749Ensembl.1
Natural variantiVAR_022189122S → R1 PublicationCorresponds to variant dbSNP:rs28359608Ensembl.1
Natural variantiVAR_022190164D → N1 PublicationCorresponds to variant dbSNP:rs28359632Ensembl.1
Natural variantiVAR_022191234I → T1 PublicationCorresponds to variant dbSNP:rs28359644Ensembl.1
Natural variantiVAR_022192239A → T1 PublicationCorresponds to variant dbSNP:rs28359647Ensembl.1
Natural variantiVAR_022193277R → W1 PublicationCorresponds to variant dbSNP:rs28359655Ensembl.1
Natural variantiVAR_022194322K → Q1 PublicationCorresponds to variant dbSNP:rs1933631Ensembl.1
Natural variantiVAR_022195327H → Q1 PublicationCorresponds to variant dbSNP:rs28359684Ensembl.1
Natural variantiVAR_022196342E → K1 PublicationCorresponds to variant dbSNP:rs16852652Ensembl.1
Natural variantiVAR_022197458R → W1 PublicationCorresponds to variant dbSNP:rs28359688Ensembl.1
Natural variantiVAR_022198522R → W1 PublicationCorresponds to variant dbSNP:rs12731961Ensembl.1
Natural variantiVAR_022199611M → I1 PublicationCorresponds to variant dbSNP:rs16852683Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007553292 – 318SSPEW…DGEFW → R in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022799 mRNA Translation: AAB80762.1
AB038463 mRNA Translation: BAA92137.1
AY874864 Genomic DNA Translation: AAW49735.1
AL512328 Genomic DNA No translation available.
FO393421 Genomic DNA No translation available.
CCDSiCCDS1586.1 [O14815-1]
CCDS31053.1 [O14815-2]
RefSeqiNP_001306605.1, NM_001319676.1
NP_006606.1, NM_006615.2 [O14815-1]
NP_057536.1, NM_016452.2 [O14815-2]
UniGeneiHs.498021

Genome annotation databases

EnsembliENST00000271971; ENSP00000271971; ENSG00000135773 [O14815-1]
ENST00000354537; ENSP00000346538; ENSG00000135773 [O14815-2]
GeneIDi10753
KEGGihsa:10753
UCSCiuc001htz.2 human [O14815-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022799 mRNA Translation: AAB80762.1
AB038463 mRNA Translation: BAA92137.1
AY874864 Genomic DNA Translation: AAW49735.1
AL512328 Genomic DNA No translation available.
FO393421 Genomic DNA No translation available.
CCDSiCCDS1586.1 [O14815-1]
CCDS31053.1 [O14815-2]
RefSeqiNP_001306605.1, NM_001319676.1
NP_006606.1, NM_006615.2 [O14815-1]
NP_057536.1, NM_016452.2 [O14815-2]
UniGeneiHs.498021

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZIVX-ray2.31A28-347[»]
2P0RX-ray2.50A/B10-340[»]
ProteinModelPortaliO14815
SMRiO14815
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115976, 2 interactors
STRINGi9606.ENSP00000271971

Protein family/group databases

MEROPSiC02.006

PTM databases

iPTMnetiO14815
PhosphoSitePlusiO14815

Polymorphism and mutation databases

BioMutaiCAPN9

Proteomic databases

EPDiO14815
PaxDbiO14815
PeptideAtlasiO14815
PRIDEiO14815
ProteomicsDBi48253
48254 [O14815-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271971; ENSP00000271971; ENSG00000135773 [O14815-1]
ENST00000354537; ENSP00000346538; ENSG00000135773 [O14815-2]
GeneIDi10753
KEGGihsa:10753
UCSCiuc001htz.2 human [O14815-1]

Organism-specific databases

CTDi10753
DisGeNETi10753
EuPathDBiHostDB:ENSG00000135773.12
GeneCardsiCAPN9
HGNCiHGNC:1486 CAPN9
HPAiCAB033483
HPA020398
MIMi606401 gene
neXtProtiNX_O14815
OpenTargetsiENSG00000135773
PharmGKBiPA26066
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiO14815
KOiK08578
OMAiINLTMNI
OrthoDBiEOG091G049E
PhylomeDBiO14815
TreeFamiTF314748

Enzyme and pathway databases

BRENDAi3.4.22.B29 2681
ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Miscellaneous databases

ChiTaRSiCAPN9 human
EvolutionaryTraceiO14815
GeneWikiiCAPN9
GenomeRNAii10753
PROiPR:O14815
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135773 Expressed in 104 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_CAPN9
ExpressionAtlasiO14815 baseline and differential
GenevisibleiO14815 HS

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029542 CAPN9
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF385 PTHR10183:SF385, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAN9_HUMAN
AccessioniPrimary (citable) accession number: O14815
Secondary accession number(s): B1APS1, B1AQI0, Q9NS74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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