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Protein

DNA-directed RNA polymerase III subunit RPC1

Gene

POLR3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.By similarity2 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1By similarity1
Metal bindingi72Zinc 1By similarity1
Metal bindingi79Zinc 1By similarity1
Metal bindingi82Zinc 1By similarity1
Metal bindingi109Zinc 2By similarity1
Metal bindingi112Zinc 2By similarity1
Metal bindingi156Zinc 2By similarity1
Metal bindingi499Magnesium; catalyticBy similarity1
Metal bindingi501Magnesium; catalyticBy similarity1
Metal bindingi503Magnesium; catalyticBy similarity1

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • DNA binding Source: InterPro
  • DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB
  • positive regulation of interferon-beta production Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB
  • transcription by RNA polymerase III Source: GOC

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processAntiviral defense, Immunity, Innate immunity, Transcription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC1 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C1
Alternative name(s):
DNA-directed RNA polymerase III largest subunit
DNA-directed RNA polymerase III subunit A
RNA polymerase III 155 kDa subunit
Short name:
RPC155
RNA polymerase III subunit C160
Gene namesi
Name:POLR3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000148606.12
HGNCiHGNC:30074 POLR3A
MIMi614258 gene
neXtProtiNX_O14802

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Involvement in diseasei

Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7)4 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability.
See also OMIM:607694
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07233891P → L in HLD7. 1 Publication1
Natural variantiVAR_072339310W → C in HLD7. 1 Publication1
Natural variantiVAR_066516372D → N in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608673EnsemblClinVar.1
Natural variantiVAR_072340387A → G in HLD7. 1 Publication1
Natural variantiVAR_066517558F → L in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608668EnsemblClinVar.1
Natural variantiVAR_072341602S → R in HLD7. 1 PublicationCorresponds to variant dbSNP:rs762708292Ensembl.1
Natural variantiVAR_066518636S → Y in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608676EnsemblClinVar.1
Natural variantiVAR_066519672G → E in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608670EnsemblClinVar.1
Natural variantiVAR_066520724C → Y in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608679EnsemblClinVar.1
Natural variantiVAR_066521775N → I in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608672EnsemblClinVar.1
Natural variantiVAR_072342804I → T in HLD7. 1 Publication1
Natural variantiVAR_066522852M → V in HLD7. 2 PublicationsCorresponds to variant dbSNP:rs267608671EnsemblClinVar.1
Natural variantiVAR_067004897I → N in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608681EnsemblClinVar.1
Natural variantiVAR_0665231005R → C in HLD7. 2 PublicationsCorresponds to variant dbSNP:rs267608682EnsemblClinVar.1
Natural variantiVAR_0665241247T → TT in HLD7. 1 Publication1
Natural variantiVAR_0723431261E → K in HLD7. 1 PublicationCorresponds to variant dbSNP:rs371703979Ensembl.1

Keywords - Diseasei

Disease mutation, Leukodystrophy

Organism-specific databases

DisGeNETi11128
GeneReviewsiPOLR3A
MalaCardsiPOLR3A
MIMi607694 phenotype
OpenTargetsiENSG00000148606
Orphaneti88637 Hypomyelination - hypogonadotropic hypogonadism - hypodontia
77295 Odontoleukodystrophy
PharmGKBiPA134900426

Polymorphism and mutation databases

BioMutaiPOLR3A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739471 – 1390DNA-directed RNA polymerase III subunit RPC1Add BLAST1390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei445N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO14802
MaxQBiO14802
PaxDbiO14802
PeptideAtlasiO14802
PRIDEiO14802
ProteomicsDBi48248

PTM databases

iPTMnetiO14802
PhosphoSitePlusiO14802

Expressioni

Tissue specificityi

Expressed in the brain, in the cortex and the white matter (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000148606
CleanExiHS_POLR3A
ExpressionAtlasiO14802 baseline and differential
GenevisibleiO14802 HS

Organism-specific databases

HPAiHPA037926

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

BioGridi116301, 73 interactors
IntActiO14802, 17 interactors
STRINGi9606.ENSP00000361446

Structurei

3D structure databases

ProteinModelPortaliO14802
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni844 – 856Bridging helixBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0260 Eukaryota
COG0086 LUCA
GeneTreeiENSGT00920000149105
HOGENOMiHOG000222974
HOVERGENiHBG004339
InParanoidiO14802
KOiK03018
OMAiAVCPPYN
OrthoDBiEOG091G0FNO
PhylomeDBiO14802
TreeFamiTF103054

Family and domain databases

CDDicd02736 RNAP_III_Rpc1_C, 1 hit
cd02583 RNAP_III_RPC1_N, 1 hit
Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR035698 RNAP_III_Rpc1_C
IPR035697 RNAP_III_RPC1_N
IPR038120 Rpb1_funnel_sf
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit

Sequencei

Sequence statusi: Complete.

O14802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP
60 70 80 90 100
LLYGVLDHRM GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA
110 120 130 140 150
VIGILQMICK TCCHIMLSQE EKKQFLDYLK RPGLTYLQKR GLKKKISDKC
160 170 180 190 200
RKKNICHHCG AFNGTVKKCG LLKIIHEKYK TNKKVVDPIV SNFLQSFETA
210 220 230 240 250
IEHNKEVEPL LGRAQENLNP LVVLNLFKRI PAEDVPLLLM NPEAGKPSDL
260 270 280 290 300
ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG
310 320 330 340 350
AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG
360 370 380 390 400
RFRGNLSGKR VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN
410 420 430 440 450
INFLRKLVQN GPEVHPGANF IQQRHTQMKR FLKYGNREKM AQELKYGDIV
460 470 480 490 500
ERHLIDGDVV LFNRQPSLHK LSIMAHLARV KPHRTFRFNE CVCTPYNADF
510 520 530 540 550
DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA AIQDFLTGAY
560 570 580 590 600
LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI
610 620 630 640 650
FSVILRPSDD NPVRANLRTK GKQYCGKGED LCANDSYVTI QNSELMSGSM
660 670 680 690 700
DKGTLGSGSK NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI
710 720 730 740 750
GDVTPGQGLL KAKYELLNAG YKKCDEYIEA LNTGKLQQQP GCTAEETLEA
760 770 780 790 800
LILKELSVIR DHAGSACLRE LDKSNSPLTM ALCGSKGSFI NISQMIACVG
810 820 830 840 850
QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS GLTPTEFFFH
860 870 880 890 900
TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF
910 920 930 940 950
IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCPSEPAL SKNELILTTE
960 970 980 990 1000
SIMKKSEFLC CQDSFLQEIK KFIKGVSEKI KKTRDKYGIN DNGTTEPRVL
1010 1020 1030 1040 1050
YQLDRITPTQ VEKFLETCRD KYMRAQMEPG SAVGALCAQS IGEPGTQMTL
1060 1070 1080 1090 1100
KTFHFAGVAS MNITLGVPRI KEIINASKAI STPIITAQLD KDDDADYARL
1110 1120 1130 1140 1150
VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL RLEVNAETVR
1160 1170 1180 1190 1200
YSICTSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV
1210 1220 1230 1240 1250
VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN
1260 1270 1280 1290 1300
TYEVEKTLGI EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV
1310 1320 1330 1340 1350
LGITRFGLAK MKESVLMLAS FEKTADHLFD AAYFGQKDSV CGVSECIIMG
1360 1370 1380 1390
IPMNIGTGLF KLLHKADRDP NPPKRPLIFD TNEFHIPLVT
Length:1,390
Mass (Da):155,641
Last modified:September 23, 2008 - v2
Checksum:i56F30900848E3DB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15I → T in AAB86536 (PubMed:9331371).Curated1
Sequence conflicti262I → F in AAB86536 (PubMed:9331371).Curated1
Sequence conflicti283L → P in AAM12029 (PubMed:12384934).Curated1
Sequence conflicti1056A → G in AAB86536 (PubMed:9331371).Curated1
Sequence conflicti1275M → MV in AAB86536 (PubMed:9331371).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07233891P → L in HLD7. 1 Publication1
Natural variantiVAR_072339310W → C in HLD7. 1 Publication1
Natural variantiVAR_066516372D → N in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608673EnsemblClinVar.1
Natural variantiVAR_072340387A → G in HLD7. 1 Publication1
Natural variantiVAR_066517558F → L in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608668EnsemblClinVar.1
Natural variantiVAR_051873582R → L. Corresponds to variant dbSNP:rs34588967EnsemblClinVar.1
Natural variantiVAR_072341602S → R in HLD7. 1 PublicationCorresponds to variant dbSNP:rs762708292Ensembl.1
Natural variantiVAR_066518636S → Y in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608676EnsemblClinVar.1
Natural variantiVAR_066519672G → E in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608670EnsemblClinVar.1
Natural variantiVAR_051874713K → N. Corresponds to variant dbSNP:rs35354908Ensembl.1
Natural variantiVAR_066520724C → Y in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608679EnsemblClinVar.1
Natural variantiVAR_066521775N → I in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608672EnsemblClinVar.1
Natural variantiVAR_072342804I → T in HLD7. 1 Publication1
Natural variantiVAR_066522852M → V in HLD7. 2 PublicationsCorresponds to variant dbSNP:rs267608671EnsemblClinVar.1
Natural variantiVAR_067004897I → N in HLD7. 1 PublicationCorresponds to variant dbSNP:rs267608681EnsemblClinVar.1
Natural variantiVAR_0665231005R → C in HLD7. 2 PublicationsCorresponds to variant dbSNP:rs267608682EnsemblClinVar.1
Natural variantiVAR_0665241247T → TT in HLD7. 1 Publication1
Natural variantiVAR_0723431261E → K in HLD7. 1 PublicationCorresponds to variant dbSNP:rs371703979Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021351 mRNA Translation: AAB86536.1
AL512628 Genomic DNA No translation available.
CH471083 Genomic DNA Translation: EAW54617.1
BC041089 mRNA Translation: AAH41089.1
AY091459 mRNA Translation: AAM12029.1
CCDSiCCDS7354.1
RefSeqiNP_008986.2, NM_007055.3
UniGeneiHs.436896

Genome annotation databases

EnsembliENST00000372371; ENSP00000361446; ENSG00000148606
GeneIDi11128
KEGGihsa:11128
UCSCiuc001jzn.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRPC1_HUMAN
AccessioniPrimary (citable) accession number: O14802
Secondary accession number(s): Q8IW34, Q8TCW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 23, 2008
Last modified: June 20, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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