SH2D1B

Functionⁱ

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation (By similarity).By similarity1 Publication2 Publications

GO - Molecular functionⁱ

protein binding, bridging
Source: UniProtKB
Traceable author statementⁱ
- 16425036

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
innate immune response Source: UniProtKB-KW
leukocyte activation involved in immune response Source: UniProtKB
positive regulation of innate immune response Source: UniProtKB
positive regulation of natural killer cell mediated immunity Source: UniProtKB
regulation of immune response Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Adaptive immunity, Immunity, Innate immunity

Biological process

Adaptive immunity, Immunity, Innate immunity

Enzyme and pathway databases

Reactomeⁱ	R-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
SignaLinkⁱ	O14796

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SH2 domain-containing protein 1B Alternative name(s): EWS/FLI1-activated transcript 2 Short name: EAT-2
Gene namesⁱ	Name:SH2D1B Synonyms:EAT2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000198574.5
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30416 SH2D1B
MIMⁱ	608510 gene
neXtProtⁱ	NX_O14796

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	127	Y → F: No stimulation of granule polarization during NK-mediated cytoxicity, abolishes activation of CD244/2B4-mediated cytotoxicity, abolishes augmentation of CD244/2B4-triggered PLCG1 phosphorylation and ERK activation. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	117157
Open Targets More...OpenTargetsⁱ	ENSG00000198574
PharmGKBⁱ	PA142670926

Polymorphism and mutation databases

BioMutaⁱ	SH2D1B

BioMutaⁱ

SH2D1B

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000097724	1 – 132	SH2 domain-containing protein 1BAdd BLAST		132

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	127	Phosphotyrosine1 Publication		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	O14796
PeptideAtlas More...PeptideAtlasⁱ	O14796
PRIDEⁱ	O14796
ProteomicsDBⁱ	48245 48246 [O14796-2]

PTM databases

iPTMnetⁱ	O14796
PhosphoSitePlusⁱ	O14796

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000198574 Expressed in 97 organ(s), highest expression level in leukocyte
CleanExⁱ	HS_SH2D1B
Genevisibleⁱ	O14796 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA054791

HPAⁱ

HPA054791

Interactionⁱ

Subunit structureⁱ

Binds to the phosphorylated receptors CD84, SLAMF1, LY9 and CD244. Does not bind to non-phosphorylated SLAMF1 (PubMed:11689425). Interacts with SLAMF7 (via ITSM phosphorylated on 'Tyr-304'). Interacts with Src kinases HCK, LYN, FYN, FGR and LCK (via kinase domains) (By similarity). Interacts (phosphorylated at Tyr-127) with PLCG1.By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
	Q53HF2	3	EBI-3923013,EBI-877761
AR	P10275	3	EBI-3923013,EBI-608057
CD244	Q9BZW8	7	EBI-3923013,EBI-1580565
CD84	Q9UIB8	3	EBI-3923013,EBI-6691679
GAB1	Q13480	8	EBI-3923013,EBI-517684
KIT	P10721	8	EBI-3923013,EBI-1379503
MET	P08581	6	EBI-3923013,EBI-1039152
OLIG1	Q8TAK6	2	EBI-3923013,EBI-3867416
PLCG1	P19174	2	EBI-3923013,EBI-79387
PLCG2	P16885	2	EBI-3923013,EBI-617403
SLAMF6	Q96DU3	4	EBI-3923013,EBI-14058448
TRIM54	Q9BYV2	5	EBI-3923013,EBI-2130429

GO - Molecular functionⁱ

protein binding, bridging
Source: UniProtKB
Traceable author statementⁱ
- 16425036

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	125563, 14 interactors
IntActⁱ	O14796, 31 interactors
Molecular INTeraction database More...MINTⁱ	O14796
STRINGⁱ	9606.ENSP00000356906

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	12 – 20	Combined sources	9
Beta strandⁱ	27 – 32	Combined sources	6
Beta strandⁱ	34 – 36	Combined sources	3
Beta strandⁱ	40 – 46	Combined sources	7
Beta strandⁱ	49 – 57	Combined sources	9
Beta strandⁱ	63 – 65	Combined sources	3
Beta strandⁱ	74 – 78	Combined sources	5
Helixⁱ	79 – 87	Combined sources	9
Beta strandⁱ	88 – 95	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	O14796
SMRⁱ	O14796
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	5 – 101	SH2PROSITE-ProRule annotationAdd BLAST		97

Keywords - Domainⁱ

SH2 domain

Phylogenomic databases

eggNOGⁱ	KOG0565 Eukaryota COG5411 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046904
HOGENOMⁱ	HOG000231700
HOVERGENⁱ	HBG003702
InParanoidⁱ	O14796
KEGG Orthology (KO) More...KOⁱ	K07989
OMAⁱ	PYYHGPL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ULG
PhylomeDBⁱ	O14796
TreeFamⁱ	TF343096

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd10342 SH2_SAP1, 1 hit
Gene3Dⁱ	3.30.505.10, 1 hit
InterProⁱ	View protein in InterPro IPR035049 EAT2_SH2 IPR000980 SH2 IPR036860 SH2_dom_sf
PANTHERⁱ	PTHR45419 PTHR45419, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00017 SH2, 1 hit
PRINTSⁱ	PR00401 SH2DOMAIN
SMARTⁱ	View protein in SMART SM00252 SH2, 1 hit
SUPFAMⁱ	SSF55550 SSF55550, 1 hit
PROSITEⁱ	View protein in PROSITE PS50001 SH2, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: O14796-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLPYYHGRL TKQDCETLLL KEGVDGNFLL RDSESIPGVL CLCVSFKNIV 
        60         70         80         90        100
YTYRIFREKH GYYRIQTAEG SPKQVFPSLK ELISKFEKPN QGMVVHLLKP 
       110        120        130 
IKRTSPSLRW RGLKLELETF VNSNSDYVDV LP

Length:132

Mass (Da):15,297

Last modified:September 19, 2002 - v2

Checksum:ⁱBC9FEA398B6B27DE

GO

Isoform 2 (identifier: O14796-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
67-121: Missing.

« Hide

        10         20         30         40         50
MDLPYYHGRL TKQDCETLLL KEGVDGNFLL RDSESIPGVL CLCVSFKNIV 
        60         70 
YTYRIFREKH GYYRIQNSNS DYVDVLP

Show »

Length:77

Mass (Da):9,024

Checksum:ⁱ96423A27A9B1FB57

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_051347	36	I → T. Corresponds to variant dbSNP:rs35688243Ensembl.		1
Natural variantⁱVAR_051348	122	N → K1 PublicationCorresponds to variant dbSNP:rs34001279Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_010341	67 – 121	Missing in isoform 2. 1 PublicationAdd BLAST		55

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF256653 mRNA Translation: AAL26000.1 AF403479 mRNA Translation: AAL27357.1 AF484964 mRNA Translation: AAM28522.1 AK097162 mRNA Translation: BAC04968.1 AK314743 mRNA Translation: BAG37283.1 AL512785 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW90703.1 BC022407 mRNA Translation: AAH22407.1 BC066595 mRNA Translation: AAH66595.1 AF020264 Genomic DNA Translation: AAB70927.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30928.1 [O14796-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_444512.2, NM_053282.4 [O14796-1]
UniGeneⁱ	Hs.350581

Genome annotation databases

Ensemblⁱ	ENST00000367929; ENSP00000356906; ENSG00000198574 [O14796-1]
GeneIDⁱ	117157
KEGGⁱ	hsa:117157
UCSC genome browser More...UCSCⁱ	uc001gbz.2 human [O14796-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Protein	Similar proteins		Species	Score	Length	Source
O14796	SH2 domain containing 1B		PANTR		132	UniRef90_O14796
SH2 domain containing 1B		MACMU		132
Uncharacterized protein		MACFA		132
Uncharacterized protein		COLAP		132
Uncharacterized protein		RHIRO		132
+13

Protein	Similar proteins		Species	Score	Length	Source
O14796	SH2 domain-containing protein 1B	)	MOUSE		132	UniRef50_O35324
Uncharacterized protein		MUSPF		143
SH2 domain-containing protein 1B		Enhydra lutris kenyoni		143
SH2 domain containing 1B		CANLF		134
Uncharacterized protein		SHEEP		133
+132

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF256653 mRNA Translation: AAL26000.1 AF403479 mRNA Translation: AAL27357.1 AF484964 mRNA Translation: AAM28522.1 AK097162 mRNA Translation: BAC04968.1 AK314743 mRNA Translation: BAG37283.1 AL512785 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW90703.1 BC022407 mRNA Translation: AAH22407.1 BC066595 mRNA Translation: AAH66595.1 AF020264 Genomic DNA Translation: AAB70927.1
CCDSⁱ	CCDS30928.1 [O14796-1]
RefSeqⁱ	NP_444512.2, NM_053282.4 [O14796-1]
UniGeneⁱ	Hs.350581

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5KAZ	X-ray	1.70	A	1-104	[»]
ProteinModelPortalⁱ	O14796
SMRⁱ	O14796
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	125563, 14 interactors
IntActⁱ	O14796, 31 interactors
MINTⁱ	O14796
STRINGⁱ	9606.ENSP00000356906

PTM databases

iPTMnetⁱ	O14796
PhosphoSitePlusⁱ	O14796

Polymorphism and mutation databases

BioMutaⁱ	SH2D1B

BioMutaⁱ

SH2D1B

Proteomic databases

PaxDbⁱ	O14796
PeptideAtlasⁱ	O14796
PRIDEⁱ	O14796
ProteomicsDBⁱ	48245 48246 [O14796-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	117157
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000367929; ENSP00000356906; ENSG00000198574 [O14796-1]
GeneIDⁱ	117157
KEGGⁱ	hsa:117157
UCSCⁱ	uc001gbz.2 human [O14796-1]

Organism-specific databases

CTDⁱ	117157
DisGeNETⁱ	117157
EuPathDBⁱ	HostDB:ENSG00000198574.5
GeneCardsⁱ	SH2D1B
HGNCⁱ	HGNC:30416 SH2D1B
HPAⁱ	HPA054791
MIMⁱ	608510 gene
neXtProtⁱ	NX_O14796
OpenTargetsⁱ	ENSG00000198574
PharmGKBⁱ	PA142670926
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0565 Eukaryota COG5411 LUCA
GeneTreeⁱ	ENSGT00510000046904
HOGENOMⁱ	HOG000231700
HOVERGENⁱ	HBG003702
InParanoidⁱ	O14796
KOⁱ	K07989
OMAⁱ	PYYHGPL
OrthoDBⁱ	EOG091G0ULG
PhylomeDBⁱ	O14796
TreeFamⁱ	TF343096

Enzyme and pathway databases

Reactomeⁱ	R-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
SignaLinkⁱ	O14796

Miscellaneous databases

GeneWikiⁱ	SH2D1B
GenomeRNAiⁱ	117157
Protein Ontology More...PROⁱ	PR:O14796
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000198574 Expressed in 97 organ(s), highest expression level in leukocyte
CleanExⁱ	HS_SH2D1B
Genevisibleⁱ	O14796 HS

Family and domain databases

CDDⁱ	cd10342 SH2_SAP1, 1 hit
Gene3Dⁱ	3.30.505.10, 1 hit
InterProⁱ	View protein in InterPro IPR035049 EAT2_SH2 IPR000980 SH2 IPR036860 SH2_dom_sf
PANTHERⁱ	PTHR45419 PTHR45419, 1 hit
Pfamⁱ	View protein in Pfam PF00017 SH2, 1 hit
PRINTSⁱ	PR00401 SH2DOMAIN
SMARTⁱ	View protein in SMART SM00252 SH2, 1 hit
SUPFAMⁱ	SSF55550 SSF55550, 1 hit
PROSITEⁱ	View protein in PROSITE PS50001 SH2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SH21B_HUMAN
Accessionⁱ	O14796Primary (citable) accession number: O14796 Secondary accession number(s): B2RBN6 , Q5T0L1, Q8NI18, Q969K9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
	Last sequence update:	September 19, 2002
	Last modified:	September 12, 2018
	This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations

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UniProtKB - O14796 (SH21B_HUMAN)

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SH2 domain-containing protein 1B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ