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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 1

Gene

HS3ST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147PAPS1
Binding sitei155PAPS1
Binding sitei255PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 68PAPS5
Nucleotide bindingi270 – 274PAPS5

GO - Molecular functioni

  • [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity Source: Reactome
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:HS00082-MONOMER
ReactomeiR-HSA-2022928 HS-GAG biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 1 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
Short name:
3-OST-1
Short name:
Heparan sulfate 3-O-sulfotransferase 1
Short name:
h3-OST-1
Gene namesi
Name:HS3ST1
Synonyms:3OST, 3OST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000002587.9
HGNCiHGNC:5194 HS3ST1
MIMi603244 gene
neXtProtiNX_O14792

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9957
OpenTargetsiENSG00000002587
PharmGKBiPA29467

Chemistry databases

DrugBankiDB01812 Adenosine-3'-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiHS3ST1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000003345121 – 307Heparan sulfate glucosamine 3-O-sulfotransferase 1Add BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi192N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi242N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi256 ↔ 2651 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO14792
PaxDbiO14792
PeptideAtlasiO14792
PRIDEiO14792
ProteomicsDBi48242

PTM databases

iPTMnetiO14792
PhosphoSitePlusiO14792

Expressioni

Tissue specificityi

Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta.1 Publication

Gene expression databases

BgeeiENSG00000002587 Expressed in 190 organ(s), highest expression level in female gonad
CleanExiHS_HS3ST1
ExpressionAtlasiO14792 baseline and differential
GenevisibleiO14792 HS

Organism-specific databases

HPAiHPA002237

Interactioni

Protein-protein interaction databases

BioGridi115282, 2 interactors
STRINGi9606.ENSP00000002596

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO14792
SMRiO14792
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14792

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiO14792
KOiK01024
OMAiRVHSMNP
OrthoDBiEOG091G0CS5
PhylomeDBiO14792
TreeFamiTF350755

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O14792-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAALLLGAVL LVAQPQLVPS RPAELGQQEL LRKAGTLQDD VRDGVAPNGS
60 70 80 90 100
AQQLPQTIII GVRKGGTRAL LEMLSLHPDV AAAENEVHFF DWEEHYSHGL
110 120 130 140 150
GWYLSQMPFS WPHQLTVEKT PAYFTSPKVP ERVYSMNPSI RLLLILRDPS
160 170 180 190 200
ERVLSDYTQV FYNHMQKHKP YPSIEEFLVR DGRLNVDYKA LNRSLYHVHM
210 220 230 240 250
QNWLRFFPLR HIHIVDGDRL IRDPFPEIQK VERFLKLSPQ INASNFYFNK
260 270 280 290 300
TKGFYCLRDS GRDRCLHESK GRAHPQVDPK LLNKLHEYFH EPNKKFFELV

GRTFDWH
Length:307
Mass (Da):35,773
Last modified:January 1, 1998 - v1
Checksum:iAA1052260633EA1C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PDE3E9PDE3_HUMAN
Heparan sulfate glucosamine 3-O-sul...
HS3ST1
82Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02151522P → T1 PublicationCorresponds to variant dbSNP:rs11559238Ensembl.1
Natural variantiVAR_052529295K → R. Corresponds to variant dbSNP:rs34719057Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019386 mRNA Translation: AAB84388.1
AK096823 mRNA Translation: BAG53368.1
CH471069 Genomic DNA Translation: EAW92699.1
BC057803 mRNA Translation: AAH57803.1
CCDSiCCDS3408.1
RefSeqiNP_005105.1, NM_005114.2
XP_011512215.1, XM_011513913.2
UniGeneiHs.507348
Hs.605349

Genome annotation databases

EnsembliENST00000002596; ENSP00000002596; ENSG00000002587
GeneIDi9957
KEGGihsa:9957
UCSCiuc003gmq.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019386 mRNA Translation: AAB84388.1
AK096823 mRNA Translation: BAG53368.1
CH471069 Genomic DNA Translation: EAW92699.1
BC057803 mRNA Translation: AAH57803.1
CCDSiCCDS3408.1
RefSeqiNP_005105.1, NM_005114.2
XP_011512215.1, XM_011513913.2
UniGeneiHs.507348
Hs.605349

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZRHX-ray2.10A36-307[»]
ProteinModelPortaliO14792
SMRiO14792
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115282, 2 interactors
STRINGi9606.ENSP00000002596

Chemistry databases

DrugBankiDB01812 Adenosine-3'-5'-Diphosphate

PTM databases

iPTMnetiO14792
PhosphoSitePlusiO14792

Polymorphism and mutation databases

BioMutaiHS3ST1

Proteomic databases

EPDiO14792
PaxDbiO14792
PeptideAtlasiO14792
PRIDEiO14792
ProteomicsDBi48242

Protocols and materials databases

DNASUi9957
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000002596; ENSP00000002596; ENSG00000002587
GeneIDi9957
KEGGihsa:9957
UCSCiuc003gmq.4 human

Organism-specific databases

CTDi9957
DisGeNETi9957
EuPathDBiHostDB:ENSG00000002587.9
GeneCardsiHS3ST1
H-InvDBiHIX0004096
HGNCiHGNC:5194 HS3ST1
HPAiHPA002237
MIMi603244 gene
neXtProtiNX_O14792
OpenTargetsiENSG00000002587
PharmGKBiPA29467
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiO14792
KOiK01024
OMAiRVHSMNP
OrthoDBiEOG091G0CS5
PhylomeDBiO14792
TreeFamiTF350755

Enzyme and pathway databases

BioCyciMetaCyc:HS00082-MONOMER
ReactomeiR-HSA-2022928 HS-GAG biosynthesis

Miscellaneous databases

ChiTaRSiHS3ST1 human
EvolutionaryTraceiO14792
GeneWikiiHS3ST1
GenomeRNAii9957
PROiPR:O14792
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000002587 Expressed in 190 organ(s), highest expression level in female gonad
CleanExiHS_HS3ST1
ExpressionAtlasiO14792 baseline and differential
GenevisibleiO14792 HS

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS3S1_HUMAN
AccessioniPrimary (citable) accession number: O14792
Secondary accession number(s): B3KUA6, Q6PEY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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