UniProtKB - O14792 (HS3S1_HUMAN)
Protein
Heparan sulfate glucosamine 3-O-sulfotransferase 1
Gene
HS3ST1
Organism
Homo sapiens (Human)
Status
Functioni
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.1 Publication
Catalytic activityi
- 3'-phosphoadenylyl sulfate + α-D-glucosaminyl-[heparan sulfate](n) = 3-sulfo-α-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H+EC:2.8.2.23
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 147 | PAPS | 1 | |
Binding sitei | 155 | PAPS | 1 | |
Binding sitei | 255 | PAPS | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 64 – 68 | PAPS | 5 | |
Nucleotide bindingi | 270 – 274 | PAPS | 5 |
GO - Molecular functioni
- [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity Source: GO_Central
- sulfotransferase activity Source: ProtInc
GO - Biological processi
- glycosaminoglycan biosynthetic process Source: Reactome
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00082-MONOMER |
PathwayCommonsi | O14792 |
Reactomei | R-HSA-2022928, HS-GAG biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Heparan sulfate glucosamine 3-O-sulfotransferase 1 (EC:2.8.2.23)Alternative name(s): Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 Short name: 3-OST-1 Short name: Heparan sulfate 3-O-sulfotransferase 1 Short name: h3-OST-1 |
Gene namesi | Name:HS3ST1 Synonyms:3OST, 3OST1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5194, HS3ST1 |
MIMi | 603244, gene |
neXtProti | NX_O14792 |
VEuPathDBi | HostDB:ENSG00000002587.9 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus lumen Curated
Golgi apparatus
- Golgi lumen Source: Reactome
Keywords - Cellular componenti
Golgi apparatusPathology & Biotechi
Organism-specific databases
DisGeNETi | 9957 |
OpenTargetsi | ENSG00000002587 |
PharmGKBi | PA29467 |
Miscellaneous databases
Pharosi | O14792, Tbio |
Chemistry databases
DrugBanki | DB01812, Adenosine 3',5'-diphosphate |
Genetic variation databases
BioMutai | HS3ST1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | By similarityAdd BLAST | 20 | |
ChainiPRO_0000033451 | 21 – 307 | Heparan sulfate glucosamine 3-O-sulfotransferase 1Add BLAST | 287 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 192 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 242 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 249 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 256 ↔ 265 | 1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | O14792 |
PaxDbi | O14792 |
PeptideAtlasi | O14792 |
PRIDEi | O14792 |
ProteomicsDBi | 48242 |
PTM databases
GlyGeni | O14792, 4 sites |
iPTMneti | O14792 |
PhosphoSitePlusi | O14792 |
Expressioni
Tissue specificityi
Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta.1 Publication
Gene expression databases
Bgeei | ENSG00000002587, Expressed in female gonad and 203 other tissues |
ExpressionAtlasi | O14792, baseline and differential |
Genevisiblei | O14792, HS |
Organism-specific databases
HPAi | ENSG00000002587, Tissue enhanced (brain, gallbladder, ovary) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 115282, 2 interactors |
STRINGi | 9606.ENSP00000002596 |
Miscellaneous databases
RNActi | O14792, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O14792 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O14792 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sulfotransferase 1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3704, Eukaryota |
GeneTreei | ENSGT00940000160449 |
HOGENOMi | CLU_017703_0_0_1 |
InParanoidi | O14792 |
OMAi | QVFYNHV |
OrthoDBi | 712400at2759 |
PhylomeDBi | O14792 |
TreeFami | TF350755 |
Family and domain databases
InterProi | View protein in InterPro IPR037359, NST/OST IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
PANTHERi | PTHR10605, PTHR10605, 1 hit |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O14792-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAALLLGAVL LVAQPQLVPS RPAELGQQEL LRKAGTLQDD VRDGVAPNGS
60 70 80 90 100
AQQLPQTIII GVRKGGTRAL LEMLSLHPDV AAAENEVHFF DWEEHYSHGL
110 120 130 140 150
GWYLSQMPFS WPHQLTVEKT PAYFTSPKVP ERVYSMNPSI RLLLILRDPS
160 170 180 190 200
ERVLSDYTQV FYNHMQKHKP YPSIEEFLVR DGRLNVDYKA LNRSLYHVHM
210 220 230 240 250
QNWLRFFPLR HIHIVDGDRL IRDPFPEIQK VERFLKLSPQ INASNFYFNK
260 270 280 290 300
TKGFYCLRDS GRDRCLHESK GRAHPQVDPK LLNKLHEYFH EPNKKFFELV
GRTFDWH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PDE3 | E9PDE3_HUMAN | Heparan sulfate glucosamine 3-O-sul... | HS3ST1 | 82 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021515 | 22 | P → T1 PublicationCorresponds to variant dbSNP:rs11559238Ensembl. | 1 | |
Natural variantiVAR_052529 | 295 | K → R. Corresponds to variant dbSNP:rs34719057Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF019386 mRNA Translation: AAB84388.1 AK096823 mRNA Translation: BAG53368.1 CH471069 Genomic DNA Translation: EAW92699.1 BC057803 mRNA Translation: AAH57803.1 |
CCDSi | CCDS3408.1 |
RefSeqi | NP_005105.1, NM_005114.2 XP_011512215.1, XM_011513913.2 |
Genome annotation databases
Ensembli | ENST00000002596; ENSP00000002596; ENSG00000002587 |
GeneIDi | 9957 |
KEGGi | hsa:9957 |
UCSCi | uc003gmq.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF019386 mRNA Translation: AAB84388.1 AK096823 mRNA Translation: BAG53368.1 CH471069 Genomic DNA Translation: EAW92699.1 BC057803 mRNA Translation: AAH57803.1 |
CCDSi | CCDS3408.1 |
RefSeqi | NP_005105.1, NM_005114.2 XP_011512215.1, XM_011513913.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZRH | X-ray | 2.10 | A | 36-307 | [»] | |
SMRi | O14792 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115282, 2 interactors |
STRINGi | 9606.ENSP00000002596 |
Chemistry databases
DrugBanki | DB01812, Adenosine 3',5'-diphosphate |
PTM databases
GlyGeni | O14792, 4 sites |
iPTMneti | O14792 |
PhosphoSitePlusi | O14792 |
Genetic variation databases
BioMutai | HS3ST1 |
Proteomic databases
MassIVEi | O14792 |
PaxDbi | O14792 |
PeptideAtlasi | O14792 |
PRIDEi | O14792 |
ProteomicsDBi | 48242 |
Protocols and materials databases
Antibodypediai | 1208, 120 antibodies |
DNASUi | 9957 |
Genome annotation databases
Ensembli | ENST00000002596; ENSP00000002596; ENSG00000002587 |
GeneIDi | 9957 |
KEGGi | hsa:9957 |
UCSCi | uc003gmq.4, human |
Organism-specific databases
CTDi | 9957 |
DisGeNETi | 9957 |
GeneCardsi | HS3ST1 |
HGNCi | HGNC:5194, HS3ST1 |
HPAi | ENSG00000002587, Tissue enhanced (brain, gallbladder, ovary) |
MIMi | 603244, gene |
neXtProti | NX_O14792 |
OpenTargetsi | ENSG00000002587 |
PharmGKBi | PA29467 |
VEuPathDBi | HostDB:ENSG00000002587.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3704, Eukaryota |
GeneTreei | ENSGT00940000160449 |
HOGENOMi | CLU_017703_0_0_1 |
InParanoidi | O14792 |
OMAi | QVFYNHV |
OrthoDBi | 712400at2759 |
PhylomeDBi | O14792 |
TreeFami | TF350755 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00082-MONOMER |
PathwayCommonsi | O14792 |
Reactomei | R-HSA-2022928, HS-GAG biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 9957, 2 hits in 983 CRISPR screens |
ChiTaRSi | HS3ST1, human |
EvolutionaryTracei | O14792 |
GeneWikii | HS3ST1 |
GenomeRNAii | 9957 |
Pharosi | O14792, Tbio |
PROi | PR:O14792 |
RNActi | O14792, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000002587, Expressed in female gonad and 203 other tissues |
ExpressionAtlasi | O14792, baseline and differential |
Genevisiblei | O14792, HS |
Family and domain databases
InterProi | View protein in InterPro IPR037359, NST/OST IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
PANTHERi | PTHR10605, PTHR10605, 1 hit |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HS3S1_HUMAN | |
Accessioni | O14792Primary (citable) accession number: O14792 Secondary accession number(s): B3KUA6, Q6PEY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families