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Protein

Kinetochore protein NDC80 homolog

Gene

NDC80

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).12 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • structural constituent of cytoskeleton Source: GO_Central

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SignaLinkiO14777
SIGNORiO14777

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NDC80 homolog
Alternative name(s):
Highly expressed in cancer protein
Kinetochore protein Hec1
Short name:
HsHec1
Kinetochore-associated protein 2
Retinoblastoma-associated protein HEC
Gene namesi
Name:NDC80
Synonyms:HEC, HEC1, KNTC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000080986.12
HGNCiHGNC:16909 NDC80
MIMi607272 gene
neXtProtiNX_O14777

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234E → K: Abrogates binding to RB1. 1 Publication1

Organism-specific databases

DisGeNETi10403
OpenTargetsiENSG00000080986
PharmGKBiPA162397359

Chemistry databases

ChEMBLiCHEMBL5660

Polymorphism and mutation databases

BioMutaiNDC80

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495501 – 642Kinetochore protein NDC80 homologAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei165Phosphoserine; by NEK21 Publication1
Modified residuei242PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14777
MaxQBiO14777
PaxDbiO14777
PeptideAtlasiO14777
PRIDEiO14777
ProteomicsDBi48231

PTM databases

iPTMnetiO14777
PhosphoSitePlusiO14777

Expressioni

Developmental stagei

Expression peaks in mitosis.3 Publications

Gene expression databases

BgeeiENSG00000080986 Expressed in 159 organ(s), highest expression level in liver
CleanExiHS_NDC80
ExpressionAtlasiO14777 baseline and differential
GenevisibleiO14777 HS

Organism-specific databases

HPAiHPA066330

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5 (PubMed:27156448). Interacts with CDT1; leading to kinetochore localization of CDT1 (PubMed:22581055).12 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115675, 97 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiO14777
DIPiDIP-35100N
IntActiO14777, 81 interactors
MINTiO14777
STRINGi9606.ENSP00000261597

Structurei

Secondary structure

1642
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO14777
SMRiO14777
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14777

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 445Interaction with the N-terminus of CDCA1Add BLAST445
Regioni1 – 250Nuclear localizationAdd BLAST250
Regioni128 – 251Interaction with RB1Add BLAST124
Regioni251 – 618Interaction with NEK2 and ZWINT1 PublicationAdd BLAST368
Regioni251 – 431Interaction with SMC1AAdd BLAST181
Regioni361 – 547Interaction with PSMC2 and SMC1AAdd BLAST187
Regioni446 – 642Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplexAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili261 – 403Sequence analysisAdd BLAST143
Coiled coili458 – 642Sequence analysisAdd BLAST185

Sequence similaritiesi

Belongs to the NDC80/HEC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0995 Eukaryota
COG5185 LUCA
GeneTreeiENSGT00390000018386
HOGENOMiHOG000012981
HOVERGENiHBG081861
InParanoidiO14777
KOiK11547
OMAiKSPTQKD
OrthoDBiEOG091G046M
PhylomeDBiO14777
TreeFamiTF101177

Family and domain databases

Gene3Di1.10.418.30, 1 hit
InterProiView protein in InterPro
IPR005550 Kinetochore_Ndc80
IPR038273 Ndc80_sf
PANTHERiPTHR10643 PTHR10643, 1 hit
PfamiView protein in Pfam
PF03801 Ndc80_HEC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

O14777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS
60 70 80 90 100
ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC
110 120 130 140 150
EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE
160 170 180 190 200
VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE
210 220 230 240 250
SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL
260 270 280 290 300
QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK
310 320 330 340 350
ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT
360 370 380 390 400
RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK
410 420 430 440 450
YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL
460 470 480 490 500
VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV
510 520 530 540 550
RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL
560 570 580 590 600
SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL
610 620 630 640
EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE
Length:642
Mass (Da):73,913
Last modified:January 1, 1998 - v1
Checksum:i6A82DA4D8B77ECDC
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GYM9V9GYM9_HUMAN
Kinetochore protein NDC80 homolog
NDC80
168Annotation score:
I3L4G3I3L4G3_HUMAN
Kinetochore protein NDC80 homolog
NDC80
141Annotation score:
I3L0P0I3L0P0_HUMAN
Kinetochore protein NDC80 homolog
NDC80
32Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02743666S → A. Corresponds to variant dbSNP:rs16943490Ensembl.1
Natural variantiVAR_027437348E → D. Corresponds to variant dbSNP:rs12456560Ensembl.1
Natural variantiVAR_027438605A → P. Corresponds to variant dbSNP:rs9051Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017790 mRNA Translation: AAB80726.1
BC010171 mRNA No translation available.
CCDSiCCDS11827.1
RefSeqiNP_006092.1, NM_006101.2
UniGeneiHs.414407

Genome annotation databases

EnsembliENST00000261597; ENSP00000261597; ENSG00000080986
GeneIDi10403
KEGGihsa:10403
UCSCiuc002kli.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017790 mRNA Translation: AAB80726.1
BC010171 mRNA No translation available.
CCDSiCCDS11827.1
RefSeqiNP_006092.1, NM_006101.2
UniGeneiHs.414407

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IGPX-ray1.80A81-196[»]
2VE7X-ray2.88A/B80-286[»]
3IZ0electron microscopy-C/E80-286[»]
ProteinModelPortaliO14777
SMRiO14777
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115675, 97 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiO14777
DIPiDIP-35100N
IntActiO14777, 81 interactors
MINTiO14777
STRINGi9606.ENSP00000261597

Chemistry databases

ChEMBLiCHEMBL5660

PTM databases

iPTMnetiO14777
PhosphoSitePlusiO14777

Polymorphism and mutation databases

BioMutaiNDC80

Proteomic databases

EPDiO14777
MaxQBiO14777
PaxDbiO14777
PeptideAtlasiO14777
PRIDEiO14777
ProteomicsDBi48231

Protocols and materials databases

DNASUi10403
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261597; ENSP00000261597; ENSG00000080986
GeneIDi10403
KEGGihsa:10403
UCSCiuc002kli.3 human

Organism-specific databases

CTDi10403
DisGeNETi10403
EuPathDBiHostDB:ENSG00000080986.12
GeneCardsiNDC80
HGNCiHGNC:16909 NDC80
HPAiHPA066330
MIMi607272 gene
neXtProtiNX_O14777
OpenTargetsiENSG00000080986
PharmGKBiPA162397359
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0995 Eukaryota
COG5185 LUCA
GeneTreeiENSGT00390000018386
HOGENOMiHOG000012981
HOVERGENiHBG081861
InParanoidiO14777
KOiK11547
OMAiKSPTQKD
OrthoDBiEOG091G046M
PhylomeDBiO14777
TreeFamiTF101177

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SignaLinkiO14777
SIGNORiO14777

Miscellaneous databases

ChiTaRSiNDC80 human
EvolutionaryTraceiO14777
GeneWikiiNDC80
GenomeRNAii10403
PROiPR:O14777
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000080986 Expressed in 159 organ(s), highest expression level in liver
CleanExiHS_NDC80
ExpressionAtlasiO14777 baseline and differential
GenevisibleiO14777 HS

Family and domain databases

Gene3Di1.10.418.30, 1 hit
InterProiView protein in InterPro
IPR005550 Kinetochore_Ndc80
IPR038273 Ndc80_sf
PANTHERiPTHR10643 PTHR10643, 1 hit
PfamiView protein in Pfam
PF03801 Ndc80_HEC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDC80_HUMAN
AccessioniPrimary (citable) accession number: O14777
Secondary accession number(s): Q6PJX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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