UniProtKB - O14776 (TCRG1_HUMAN)
Protein
Transcription elongation regulator 1
Gene
TCERG1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.2 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
- proline-rich region binding Source: Ensembl
- RNA binding Source: UniProtKB
- RNA polymerase binding Source: GO_Central
- RNA polymerase II repressing transcription factor binding Source: BHF-UCL
- transcription coactivator activity Source: ProtInc
- transcription coregulator activity Source: GO_Central
- transcription corepressor activity Source: BHF-UCL
- transcription elongation regulator activity Source: ARUK-UCL
- ubiquitin-like protein conjugating enzyme binding Source: ARUK-UCL
GO - Biological processi
- mRNA processing Source: ARUK-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- negative regulation of transcription elongation from RNA polymerase II promoter Source: ARUK-UCL
- positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
- positive regulation of transcription elongation from RNA polymerase II promoter Source: ARUK-UCL
- RNA splicing Source: ARUK-UCL
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | O14776 |
SignaLinki | O14776 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation regulator 1Alternative name(s): TATA box-binding protein-associated factor 2S Transcription factor CA150 |
Gene namesi | Name:TCERG1 Synonyms:CA150, TAF2S |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000113649.11 |
HGNCi | HGNC:15630, TCERG1 |
MIMi | 605409, gene |
neXtProti | NX_O14776 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Nucleus
- nuclear speck Source: ARUK-UCL
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 148 – 150 | YYY → AAA: Reduces repression of transcription by 35%. Reduces repression of transcription by 63%; when associated with 446-AAA-448. 1 Publication | 3 | |
Mutagenesisi | 446 – 448 | YYY → AAA: Loss of interaction with SF1. Reduces repression of transcription by 35%. Reduces repression of transcription by 63%; when associated with 148-AAA-150. 1 Publication | 3 | |
Mutagenesisi | 545 – 547 | FFY → AAA: No effect. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 10915 |
OpenTargetsi | ENSG00000113649 |
PharmGKBi | PA38007 |
Miscellaneous databases
Pharosi | O14776, Tbio |
Polymorphism and mutation databases
BioMutai | TCERG1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076063 | 1 – 1098 | Transcription elongation regulator 1Add BLAST | 1098 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 28 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 30 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 41 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 48 | Asymmetric dimethylarginineCombined sources | 1 | |
Cross-linki | 503 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 507 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 608 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 638 | PhosphoserineCombined sources | 1 | |
Modified residuei | 834 | PhosphoserineCombined sources | 1 | |
Modified residuei | 933 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O14776 |
jPOSTi | O14776 |
MassIVEi | O14776 |
MaxQBi | O14776 |
PaxDbi | O14776 |
PeptideAtlasi | O14776 |
PRIDEi | O14776 |
ProteomicsDBi | 48229 [O14776-1] 48230 [O14776-2] |
PTM databases
CarbonylDBi | O14776 |
iPTMneti | O14776 |
MetOSitei | O14776 |
PhosphoSitePlusi | O14776 |
SwissPalmi | O14776 |
Expressioni
Tissue specificityi
Detected in brain neurons.1 Publication
Inductioni
Up-regulated in brain tissue from patients with Huntington disease.1 Publication
Gene expression databases
Bgeei | ENSG00000113649, Expressed in intestine and 185 other tissues |
ExpressionAtlasi | O14776, baseline and differential |
Genevisiblei | O14776, HS |
Organism-specific databases
HPAi | ENSG00000113649, Low tissue specificity |
Interactioni
Subunit structurei
Binds formin (By similarity).
Interacts (via the second WW domain) with TREX1 (via proline-rich region) (By similarity). Binds RNA polymerase II, HD and SF1.
By similarity5 PublicationsBinary interactionsi
Hide detailsO14776
With | #Exp. | IntAct |
---|---|---|
BARD1 [Q99728] | 2 | EBI-473271,EBI-473181 |
HTT [P42858] | 9 | EBI-473271,EBI-466029 |
itself | 4 | EBI-473271,EBI-473271 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- proline-rich region binding Source: Ensembl
- RNA polymerase binding Source: GO_Central
- RNA polymerase II repressing transcription factor binding Source: BHF-UCL
- ubiquitin-like protein conjugating enzyme binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 116120, 99 interactors |
CORUMi | O14776 |
DIPi | DIP-32504N |
IntActi | O14776, 32 interactors |
MINTi | O14776 |
STRINGi | 9606.ENSP00000296702 |
Miscellaneous databases
RNActi | O14776, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O14776 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O14776 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 131 – 164 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 429 – 462 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 528 – 561 | WW 3PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 659 – 712 | FF 1Add BLAST | 54 | |
Domaini | 725 – 779 | FF 2Add BLAST | 55 | |
Domaini | 791 – 846 | FF 3Add BLAST | 56 | |
Domaini | 896 – 952 | FF 4Add BLAST | 57 | |
Domaini | 954 – 1010 | FF 5Add BLAST | 57 | |
Domaini | 1012 – 1077 | FF 6Add BLAST | 66 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 184 – 244 | Sequence analysisAdd BLAST | 61 | |
Coiled coili | 606 – 655 | Sequence analysisAdd BLAST | 50 | |
Coiled coili | 844 – 906 | Sequence analysisAdd BLAST | 63 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 626 – 630 | Nuclear localization signalSequence analysis | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 32 – 132 | Pro-richAdd BLAST | 101 | |
Compositional biasi | 169 – 258 | Ala/Gln-richAdd BLAST | 90 | |
Compositional biasi | 260 – 331 | Thr-richAdd BLAST | 72 | |
Compositional biasi | 328 – 378 | Pro-richAdd BLAST | 51 | |
Compositional biasi | 454 – 515 | Glu-richAdd BLAST | 62 | |
Compositional biasi | 1083 – 1087 | Poly-Pro | 5 |
Domaini
The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE]2-5-[FWY]-[DE]2-5.
The WW domains bind Pro-rich domains.
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0155, Eukaryota |
GeneTreei | ENSGT00940000157770 |
HOGENOMi | CLU_008684_0_0_1 |
InParanoidi | O14776 |
OMAi | MMNGPIG |
OrthoDBi | 1388955at2759 |
PhylomeDBi | O14776 |
TreeFami | TF317748 |
Family and domain databases
CDDi | cd00201, WW, 3 hits |
DisProti | DP01893 |
Gene3Di | 1.10.10.440, 6 hits |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF01846, FF, 6 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00441, FF, 6 hits SM00456, WW, 3 hits |
SUPFAMi | SSF51045, SSF51045, 3 hits SSF81698, SSF81698, 5 hits |
PROSITEi | View protein in PROSITE PS51676, FF, 6 hits PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O14776-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAERGGDGGE SERFNPGELR MAQQQALRFR GPAPPPNAVM RGPPPLMRPP
60 70 80 90 100
PPFGMMRGPP PPPRPPFGRP PFDPNMPPMP PPGGIPPPMG PPHLQRPPFM
110 120 130 140 150
PPPMSSMPPP PGMMFPPGMP PVTAPGTPAL PPTEEIWVEN KTPDGKVYYY
160 170 180 190 200
NARTRESAWT KPDGVKVIQQ SELTPMLAAQ AQVQAQAQAQ AQAQAQAQAQ
210 220 230 240 250
AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQVQAQVQAQ
260 270 280 290 300
VQAQAVGAST PTTSSPAPAV STSTSSSTPS STTSTTTTAT SVAQTVSTPT
310 320 330 340 350
TQDQTPSSAV SVATPTVSVS TPAPTATPVQ TVPQPHPQTL PPAVPHSVPQ
360 370 380 390 400
PTTAIPAFPP VMVPPFRVPL PGMPIPLPGV AMMQIVSCPY VKTVATTKTG
410 420 430 440 450
VLPGMAPPIV PMIHPQVAIA ASPATLAGAT AVSEWTEYKT ADGKTYYYNN
460 470 480 490 500
RTLESTWEKP QELKEKEKLE EKIKEPIKEP SEEPLPMETE EEDPKEEPIK
510 520 530 540 550
EIKEEPKEEE MTEEEKAAQK AKPVATAPIP GTPWCVVWTG DERVFFYNPT
560 570 580 590 600
TRLSMWDRPD DLIGRADVDK IIQEPPHKKG MEELKKLRHP TPTMLSIQKW
610 620 630 640 650
QFSMSAIKEE QELMEEINED EPVKAKKRKR DDNKDIDSEK EAAMEAEIKA
660 670 680 690 700
ARERAIVPLE ARMKQFKDML LERGVSAFST WEKELHKIVF DPRYLLLNPK
710 720 730 740 750
ERKQVFDQYV KTRAEEERRE KKNKIMQAKE DFKKMMEEAK FNPRATFSEF
760 770 780 790 800
AAKHAKDSRF KAIEKMKDRE ALFNEFVAAA RKKEKEDSKT RGEKIKSDFF
810 820 830 840 850
ELLSNHHLDS QSRWSKVKDK VESDPRYKAV DSSSMREDLF KQYIEKIAKN
860 870 880 890 900
LDSEKEKELE RQARIEASLR EREREVQKAR SEQTKEIDRE REQHKREEAI
910 920 930 940 950
QNFKALLSDM VRSSDVSWSD TRRTLRKDHR WESGSLLERE EKEKLFNEHI
960 970 980 990 1000
EALTKKKREH FRQLLDETSA ITLTSTWKEV KKIIKEDPRC IKFSSSDRKK
1010 1020 1030 1040 1050
QREFEEYIRD KYITAKADFR TLLKETKFIT YRSKKLIQES DQHLKDVEKI
1060 1070 1080 1090
LQNDKRYLVL DCVPEERRKL IVAYVDDLDR RGPPPPPTAS EPTRRSTK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V220 | G3V220_HUMAN | Transcription elongation regulator ... | TCERG1 hCG_38116 | 1,032 | Annotation score: |
Sequence cautioni
The sequence BAD93147 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 324 | P → R in AAB80727 (PubMed:9315662).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_026933 | 379 – 399 | Missing in isoform 2. 2 PublicationsAdd BLAST | 21 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017789 mRNA Translation: AAB80727.1 AB209910 mRNA Translation: BAD93147.1 Different initiation. BC111727 mRNA Translation: AAI11728.1 |
CCDSi | CCDS4282.1 [O14776-1] CCDS43379.1 [O14776-2] |
PIRi | T08599 |
RefSeqi | NP_001035095.1, NM_001040006.1 [O14776-2] NP_006697.2, NM_006706.3 [O14776-1] |
Genome annotation databases
Ensembli | ENST00000296702; ENSP00000296702; ENSG00000113649 [O14776-1] ENST00000394421; ENSP00000377943; ENSG00000113649 [O14776-2] |
GeneIDi | 10915 |
KEGGi | hsa:10915 |
UCSCi | uc003lob.4, human [O14776-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017789 mRNA Translation: AAB80727.1 AB209910 mRNA Translation: BAD93147.1 Different initiation. BC111727 mRNA Translation: AAI11728.1 |
CCDSi | CCDS4282.1 [O14776-1] CCDS43379.1 [O14776-2] |
PIRi | T08599 |
RefSeqi | NP_001035095.1, NM_001040006.1 [O14776-2] NP_006697.2, NM_006706.3 [O14776-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DK7 | NMR | - | A | 520-579 | [»] | |
2DOD | NMR | - | A | 651-719 | [»] | |
2DOE | NMR | - | A | 784-853 | [»] | |
2DOF | NMR | - | A | 888-959 | [»] | |
2E71 | NMR | - | A | 717-786 | [»] | |
2KIQ | NMR | - | A | 724-782 | [»] | |
2KIS | NMR | - | A | 659-724 | [»] | |
2MW9 | NMR | - | A | 428-464 | [»] | |
2MWA | NMR | - | A | 428-464 | [»] | |
2MWB | NMR | - | A | 428-464 | [»] | |
2MWD | NMR | - | A | 433-460 | [»] | |
2MWE | NMR | - | A | 433-460 | [»] | |
2MWF | NMR | - | A | 433-464 | [»] | |
2N4R | NMR | - | A | 428-464 | [»] | |
2N4S | NMR | - | A | 428-464 | [»] | |
2N4T | NMR | - | A | 428-464 | [»] | |
2N4U | NMR | - | A | 428-464 | [»] | |
2N4V | NMR | - | A | 428-464 | [»] | |
2N4W | NMR | - | A | 428-464 | [»] | |
2NNT | NMR | - | A/B/C/D | 428-464 | [»] | |
3HFH | X-ray | 2.70 | A/B | 661-845 | [»] | |
3Q1I | X-ray | 1.40 | E | 99-107 | [»] | |
4FQG | X-ray | 2.00 | A/B | 895-1081 | [»] | |
SMRi | O14776 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116120, 99 interactors |
CORUMi | O14776 |
DIPi | DIP-32504N |
IntActi | O14776, 32 interactors |
MINTi | O14776 |
STRINGi | 9606.ENSP00000296702 |
PTM databases
CarbonylDBi | O14776 |
iPTMneti | O14776 |
MetOSitei | O14776 |
PhosphoSitePlusi | O14776 |
SwissPalmi | O14776 |
Polymorphism and mutation databases
BioMutai | TCERG1 |
Proteomic databases
EPDi | O14776 |
jPOSTi | O14776 |
MassIVEi | O14776 |
MaxQBi | O14776 |
PaxDbi | O14776 |
PeptideAtlasi | O14776 |
PRIDEi | O14776 |
ProteomicsDBi | 48229 [O14776-1] 48230 [O14776-2] |
Protocols and materials databases
Antibodypediai | 15805, 181 antibodies |
Genome annotation databases
Ensembli | ENST00000296702; ENSP00000296702; ENSG00000113649 [O14776-1] ENST00000394421; ENSP00000377943; ENSG00000113649 [O14776-2] |
GeneIDi | 10915 |
KEGGi | hsa:10915 |
UCSCi | uc003lob.4, human [O14776-1] |
Organism-specific databases
CTDi | 10915 |
DisGeNETi | 10915 |
EuPathDBi | HostDB:ENSG00000113649.11 |
GeneCardsi | TCERG1 |
HGNCi | HGNC:15630, TCERG1 |
HPAi | ENSG00000113649, Low tissue specificity |
MIMi | 605409, gene |
neXtProti | NX_O14776 |
OpenTargetsi | ENSG00000113649 |
PharmGKBi | PA38007 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0155, Eukaryota |
GeneTreei | ENSGT00940000157770 |
HOGENOMi | CLU_008684_0_0_1 |
InParanoidi | O14776 |
OMAi | MMNGPIG |
OrthoDBi | 1388955at2759 |
PhylomeDBi | O14776 |
TreeFami | TF317748 |
Enzyme and pathway databases
PathwayCommonsi | O14776 |
SignaLinki | O14776 |
Miscellaneous databases
BioGRID-ORCSi | 10915, 272 hits in 849 CRISPR screens |
ChiTaRSi | TCERG1, human |
EvolutionaryTracei | O14776 |
GeneWikii | Transcription_elongation_regulator_1 |
GenomeRNAii | 10915 |
Pharosi | O14776, Tbio |
PROi | PR:O14776 |
RNActi | O14776, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000113649, Expressed in intestine and 185 other tissues |
ExpressionAtlasi | O14776, baseline and differential |
Genevisiblei | O14776, HS |
Family and domain databases
CDDi | cd00201, WW, 3 hits |
DisProti | DP01893 |
Gene3Di | 1.10.10.440, 6 hits |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF01846, FF, 6 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00441, FF, 6 hits SM00456, WW, 3 hits |
SUPFAMi | SSF51045, SSF51045, 3 hits SSF81698, SSF81698, 5 hits |
PROSITEi | View protein in PROSITE PS51676, FF, 6 hits PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCRG1_HUMAN | |
Accessioni | O14776Primary (citable) accession number: O14776 Secondary accession number(s): Q2NKN2, Q59EA1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | July 24, 2007 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references