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Protein

Acyl-coenzyme A thioesterase 8

Gene

ACOT8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).3 Publications
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression (PubMed:9153233).1 Publication

Catalytic activityi

Choloyl-CoA + H2O = cholate + CoA.2 Publications

Activity regulationi

Inhibited by CoASH (IC50=10-15 µM). Also inhibited by cysteine-reactive agents.By similarity

Kineticsi

  1. KM=10.1 µM for decanoyl-CoA1 Publication
  1. Vmax=7.1 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Charge relay systemBy similarity1
Active sitei254Charge relay systemBy similarity1
Active sitei304Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processHost-virus interaction, Peroxisome biogenesis

Enzyme and pathway databases

BRENDAi3.1.2.2 2681
3.1.2.20 2681
ReactomeiR-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
R-HSA-389887 Beta-oxidation of pristanoyl-CoA
R-HSA-390247 Beta-oxidation of very long chain fatty acids
R-HSA-9033241 Peroxisomal protein import

Chemistry databases

SwissLipidsiSLP:000000591

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 8 (EC:3.1.2.272 Publications)
Short name:
Acyl-CoA thioesterase 8
Alternative name(s):
Choloyl-coenzyme A thioesterase
HIV-Nef-associated acyl-CoA thioesterase
PTE-2
Peroxisomal acyl-coenzyme A thioester hydrolase 1
Short name:
PTE-1
Peroxisomal long-chain acyl-CoA thioesterase 1
Thioesterase II
Short name:
hACTE-III
Short name:
hACTEIII
Short name:
hTE
Gene namesi
Name:ACOT8
Synonyms:ACTEIII, PTE1, PTE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101473.16
HGNCiHGNC:15919 ACOT8
MIMi608123 gene
neXtProtiNX_O14734

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78H → A: Reduces Acyl-CoA thioesterase activity and peroxisome proliferation. 1 Publication1

Organism-specific databases

DisGeNETi10005
OpenTargetsiENSG00000101473
PharmGKBiPA33941

Polymorphism and mutation databases

BioMutaiACOT8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021521 – 319Acyl-coenzyme A thioesterase 8Add BLAST319

Proteomic databases

EPDiO14734
MaxQBiO14734
PaxDbiO14734
PeptideAtlasiO14734
PRIDEiO14734
ProteomicsDBi48196

PTM databases

iPTMnetiO14734
PhosphoSitePlusiO14734
SwissPalmiO14734

Expressioni

Tissue specificityi

Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).2 Publications

Inductioni

Regulated by peroxisome proliferator (such as Clofibrate), via the peroxisome proliferator-activated receptors (PPARs).By similarity

Gene expression databases

BgeeiENSG00000101473 Expressed in 194 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_ACOT8
ExpressionAtlasiO14734 baseline and differential
GenevisibleiO14734 HS

Organism-specific databases

HPAiCAB010261

Interactioni

Subunit structurei

Homodimer (By similarity).By similarity
(Microbial infection) Interacts with human immunodeficiency virus (HIV-1) Nef (via middle region); this interaction enhances ACOT8 Acyl-CoA thioesterase activity and occurs in a Nef myristoylation-independent manner (PubMed:9299485). According to a second report, the interaction with HIV-1 Nef occurs in a Nef myristoylation-independent manner but does not enhance ACOT8 Acyl-CoA thioesterase activity (PubMed:9153233).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115323, 18 interactors
DIPiDIP-38179N
IntActiO14734, 13 interactors
STRINGi9606.ENSP00000217455

Structurei

3D structure databases

ProteinModelPortaliO14734
SMRiO14734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi317 – 319Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG3016 Eukaryota
COG1946 LUCA
GeneTreeiENSGT00390000004207
HOGENOMiHOG000246495
HOVERGENiHBG019167
InParanoidiO14734
KOiK11992
OMAiWLLYAMD
OrthoDBiEOG091G0BLB
PhylomeDBiO14734
TreeFamiTF315124

Family and domain databases

InterProiView protein in InterPro
IPR003703 Acyl_CoA_thio
IPR029069 HotDog_dom_sf
PANTHERiPTHR11066 PTHR11066, 1 hit
SUPFAMiSSF54637 SSF54637, 2 hits
TIGRFAMsiTIGR00189 tesB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

O14734-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSPQAPEDG QGCGDRGDPP GDLRSVLVTT VLNLEPLDED LFRGRHYWVP
60 70 80 90 100
AKRLFGGQIV GQALVAAAKS VSEDVHVHSL HCYFVRAGDP KLPVLYQVER
110 120 130 140 150
TRTGSSFSVR SVKAVQHGKP IFICQASFQQ AQPSPMQHQF SMPTVPPPEE
160 170 180 190 200
LLDCETLIDQ YLRDPNLQKR YPLALNRIAA QEVPIEIKPV NPSPLSQLQR
210 220 230 240 250
MEPKQMFWVR ARGYIGEGDM KMHCCVAAYI SDYAFLGTAL LPHQWQHKVH
260 270 280 290 300
FMVSLDHSMW FHAPFRADHW MLYECESPWA GGSRGLVHGR LWRQDGVLAV
310
TCAQEGVIRV KPQVSESKL
Length:319
Mass (Da):35,914
Last modified:January 1, 1998 - v1
Checksum:i8345C6E5EABF3326
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PRD4E9PRD4_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
171Annotation score:
E9PJN0E9PJN0_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
226Annotation score:
E9PMC4E9PMC4_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
94Annotation score:
H7C5A7H7C5A7_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
209Annotation score:
E9PIS4E9PIS4_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
132Annotation score:
Q9BR14Q9BR14_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
92Annotation score:
H0Y698H0Y698_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
74Annotation score:
F6VBM3F6VBM3_HUMAN
Acyl-coenzyme A thioesterase 8
ACOT8
107Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291 – 293LWR → VWS in CAA60024 (PubMed:9153233).Curated3
Sequence conflicti319L → R in CAA60024 (PubMed:9153233).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014404 mRNA Translation: AAB71665.1
X86032 mRNA Translation: CAA60024.1
AF124264 mRNA Translation: AAD27616.1
AL008726 Genomic DNA No translation available.
BC117155 mRNA Translation: AAI17156.1
BC117157 mRNA Translation: AAI17158.1
CCDSiCCDS13378.1
PIRiJC5644
RefSeqiNP_005460.2, NM_005469.3
UniGeneiHs.444776

Genome annotation databases

EnsembliENST00000217455; ENSP00000217455; ENSG00000101473
GeneIDi10005
KEGGihsa:10005
UCSCiuc002xqa.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014404 mRNA Translation: AAB71665.1
X86032 mRNA Translation: CAA60024.1
AF124264 mRNA Translation: AAD27616.1
AL008726 Genomic DNA No translation available.
BC117155 mRNA Translation: AAI17156.1
BC117157 mRNA Translation: AAI17158.1
CCDSiCCDS13378.1
PIRiJC5644
RefSeqiNP_005460.2, NM_005469.3
UniGeneiHs.444776

3D structure databases

ProteinModelPortaliO14734
SMRiO14734
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115323, 18 interactors
DIPiDIP-38179N
IntActiO14734, 13 interactors
STRINGi9606.ENSP00000217455

Chemistry databases

SwissLipidsiSLP:000000591

PTM databases

iPTMnetiO14734
PhosphoSitePlusiO14734
SwissPalmiO14734

Polymorphism and mutation databases

BioMutaiACOT8

Proteomic databases

EPDiO14734
MaxQBiO14734
PaxDbiO14734
PeptideAtlasiO14734
PRIDEiO14734
ProteomicsDBi48196

Protocols and materials databases

DNASUi10005
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217455; ENSP00000217455; ENSG00000101473
GeneIDi10005
KEGGihsa:10005
UCSCiuc002xqa.3 human

Organism-specific databases

CTDi10005
DisGeNETi10005
EuPathDBiHostDB:ENSG00000101473.16
GeneCardsiACOT8
HGNCiHGNC:15919 ACOT8
HPAiCAB010261
MIMi608123 gene
neXtProtiNX_O14734
OpenTargetsiENSG00000101473
PharmGKBiPA33941
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3016 Eukaryota
COG1946 LUCA
GeneTreeiENSGT00390000004207
HOGENOMiHOG000246495
HOVERGENiHBG019167
InParanoidiO14734
KOiK11992
OMAiWLLYAMD
OrthoDBiEOG091G0BLB
PhylomeDBiO14734
TreeFamiTF315124

Enzyme and pathway databases

BRENDAi3.1.2.2 2681
3.1.2.20 2681
ReactomeiR-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
R-HSA-389887 Beta-oxidation of pristanoyl-CoA
R-HSA-390247 Beta-oxidation of very long chain fatty acids
R-HSA-9033241 Peroxisomal protein import

Miscellaneous databases

ChiTaRSiACOT8 human
GeneWikiiACOT8
GenomeRNAii10005
PROiPR:O14734
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101473 Expressed in 194 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_ACOT8
ExpressionAtlasiO14734 baseline and differential
GenevisibleiO14734 HS

Family and domain databases

InterProiView protein in InterPro
IPR003703 Acyl_CoA_thio
IPR029069 HotDog_dom_sf
PANTHERiPTHR11066 PTHR11066, 1 hit
SUPFAMiSSF54637 SSF54637, 2 hits
TIGRFAMsiTIGR00189 tesB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACOT8_HUMAN
AccessioniPrimary (citable) accession number: O14734
Secondary accession number(s): O15261, Q17RX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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