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Protein

Serine/threonine-protein kinase RIO3

Gene

RIOK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843).4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei290ATPBy similarity1
Active sitei406Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 265ATPBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • caspase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAntiviral defense, Immunity, Innate immunity, Ribosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO3 (EC:2.7.11.1)
Alternative name(s):
RIO kinase 3
sudD homolog
Gene namesi
Name:RIOK3
Synonyms:SUDD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000101782.14
HGNCiHGNC:11451 RIOK3
MIMi603579 gene
neXtProtiNX_O14730

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi290K → A: Decreases autophosphorylation (in vitro); abolishes inhibition of TNF-alpha-induced NF-kappaB activation; no effect on interaction with IRF3; fails to activate IFN-beta promoter and IRF3 phosphorylation. 2 Publications1

Organism-specific databases

DisGeNETi8780
OpenTargetsiENSG00000101782
PharmGKBiPA36248

Chemistry databases

ChEMBLiCHEMBL5659

Polymorphism and mutation databases

BioMutaiRIOK3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135291 – 519Serine/threonine-protein kinase RIO3Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei112PhosphoserineCombined sources1
Modified residuei122PhosphotyrosineCombined sources1
Modified residuei125PhosphoserineBy similarity1
Modified residuei127PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated (in vitro).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14730
MaxQBiO14730
PaxDbiO14730
PeptideAtlasiO14730
PRIDEiO14730
ProteomicsDBi48188
48189 [O14730-2]

PTM databases

iPTMnetiO14730
PhosphoSitePlusiO14730

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000101782 Expressed in 230 organ(s), highest expression level in amniotic fluid
CleanExiHS_RIOK3
ExpressionAtlasiO14730 baseline and differential
GenevisibleiO14730 HS

Organism-specific databases

HPAiHPA001620
HPA024184

Interactioni

Subunit structurei

Interacts with CASP10 (PubMed:19557502). Interacts with IRF3; RIOK3 probybly mediates the interaction of TBK1 with IRF3 (PubMed:24807708). Associated with 40S pre-ribosomal particles.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114309, 28 interactors
IntActiO14730, 17 interactors
STRINGi9606.ENSP00000341874

Chemistry databases

BindingDBiO14730

Structurei

3D structure databases

ProteinModelPortaliO14730
SMRiO14730
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 519Protein kinaseAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2269 Eukaryota
COG1718 LUCA
GeneTreeiENSGT00390000004370
HOGENOMiHOG000007623
HOVERGENiHBG057475
InParanoidiO14730
KOiK08872
OMAiDTRHDPY
OrthoDBiEOG091G064C
PhylomeDBiO14730
TreeFamiTF105831

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR017406 Ser/Thr_kinase_Rio3
PIRSFiPIRSF038146 Ser/Thr_PK_RIO3, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O14730-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE
60 70 80 90 100
EEAAVFPEVA VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE
110 120 130 140 150
KKFNGDSKVS ISFENYRKVH PYEDSDSSED EVDWQDTRDD PYRPAKPVPT
160 170 180 190 200
PKKGFIGKGK DITTKHDEVV CGRKNTARME NFAPEFQVGD GIGMDLKLSN
210 220 230 240 250
HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL MYKMVNSGML
260 270 280 290 300
ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK
310 320 330 340 350
NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV
360 370 380 390 400
LLKKHILVMS FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC
410 420 430 440 450
TLVHADLSEY NMLWHAGKVW LIDVSQSVEP THPHGLEFLF RDCRNVSQFF
460 470 480 490 500
QKGGVKEALS ERELFNAVSG LNITADNEAD FLAEIEALEK MNEDHVQKNG
510
RKAASFLKDD GDPPLLYDE
Length:519
Mass (Da):59,093
Last modified:February 20, 2007 - v2
Checksum:i3C3392BAC4ECB912
GO
Isoform 2 (identifier: O14730-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-451: Missing.

Note: No experimental confirmation available.
Show »
Length:516
Mass (Da):58,671
Checksum:i2CA9BC88DD00CFE5
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4E1Q4B4E1Q4_HUMAN
Serine/threonine-protein kinase RIO...
RIOK3
503Annotation score:
J3QQL5J3QQL5_HUMAN
Serine/threonine-protein kinase RIO...
RIOK3
135Annotation score:
J3QLP4J3QLP4_HUMAN
Serine/threonine-protein kinase RIO...
RIOK3
89Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti325I → H in AAC26080 (PubMed:9602165).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042355336L → V1 PublicationCorresponds to variant dbSNP:rs35401850Ensembl.1
Natural variantiVAR_042356441R → Q1 PublicationCorresponds to variant dbSNP:rs33969048Ensembl.1
Natural variantiVAR_042357447S → L1 PublicationCorresponds to variant dbSNP:rs56282762Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012164449 – 451Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013591 mRNA Translation: AAC26080.1
AC026634 Genomic DNA No translation available.
BC039729 mRNA Translation: AAH39729.1
CCDSiCCDS11877.1 [O14730-1]
RefSeqiNP_003822.2, NM_003831.4 [O14730-1]
UniGeneiHs.445511
Hs.728452

Genome annotation databases

EnsembliENST00000339486; ENSP00000341874; ENSG00000101782 [O14730-1]
ENST00000577501; ENSP00000462548; ENSG00000101782 [O14730-2]
GeneIDi8780
KEGGihsa:8780
UCSCiuc002kui.5 human [O14730-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013591 mRNA Translation: AAC26080.1
AC026634 Genomic DNA No translation available.
BC039729 mRNA Translation: AAH39729.1
CCDSiCCDS11877.1 [O14730-1]
RefSeqiNP_003822.2, NM_003831.4 [O14730-1]
UniGeneiHs.445511
Hs.728452

3D structure databases

ProteinModelPortaliO14730
SMRiO14730
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114309, 28 interactors
IntActiO14730, 17 interactors
STRINGi9606.ENSP00000341874

Chemistry databases

BindingDBiO14730
ChEMBLiCHEMBL5659

PTM databases

iPTMnetiO14730
PhosphoSitePlusiO14730

Polymorphism and mutation databases

BioMutaiRIOK3

Proteomic databases

EPDiO14730
MaxQBiO14730
PaxDbiO14730
PeptideAtlasiO14730
PRIDEiO14730
ProteomicsDBi48188
48189 [O14730-2]

Protocols and materials databases

DNASUi8780
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339486; ENSP00000341874; ENSG00000101782 [O14730-1]
ENST00000577501; ENSP00000462548; ENSG00000101782 [O14730-2]
GeneIDi8780
KEGGihsa:8780
UCSCiuc002kui.5 human [O14730-1]

Organism-specific databases

CTDi8780
DisGeNETi8780
EuPathDBiHostDB:ENSG00000101782.14
GeneCardsiRIOK3
HGNCiHGNC:11451 RIOK3
HPAiHPA001620
HPA024184
MIMi603579 gene
neXtProtiNX_O14730
OpenTargetsiENSG00000101782
PharmGKBiPA36248
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2269 Eukaryota
COG1718 LUCA
GeneTreeiENSGT00390000004370
HOGENOMiHOG000007623
HOVERGENiHBG057475
InParanoidiO14730
KOiK08872
OMAiDTRHDPY
OrthoDBiEOG091G064C
PhylomeDBiO14730
TreeFamiTF105831

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiRIOK3 human
GenomeRNAii8780
PROiPR:O14730
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101782 Expressed in 230 organ(s), highest expression level in amniotic fluid
CleanExiHS_RIOK3
ExpressionAtlasiO14730 baseline and differential
GenevisibleiO14730 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR017406 Ser/Thr_kinase_Rio3
PIRSFiPIRSF038146 Ser/Thr_PK_RIO3, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIOK3_HUMAN
AccessioniPrimary (citable) accession number: O14730
Secondary accession number(s): Q8IXN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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