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Protein

Apoptotic protease-activating factor 1

Gene

APAF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.2 Publications

Miscellaneous

Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 161ATPSequence analysis8

GO - Molecular functioni

  • ADP binding Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • cysteine-type endopeptidase activator activity involved in apoptotic process Source: GO_Central
  • heat shock protein binding Source: Ensembl
  • identical protein binding Source: Ensembl
  • nucleotide binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-111458 Formation of apoptosome
R-HSA-111459 Activation of caspases through apoptosome-mediated cleavage
R-HSA-6798695 Neutrophil degranulation
R-HSA-6803207 TP53 Regulates Transcription of Caspase Activators and Caspases
R-HSA-8953750 Transcriptional Regulation by E2F6
SABIO-RKiO14727
SignaLinkiO14727
SIGNORiO14727

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1Curated
Short name:
APAF-1
Gene namesi
Name:APAF1Imported
Synonyms:KIAA0413
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000120868.13
HGNCiHGNC:576 APAF1
MIMi602233 gene
neXtProtiNX_O14727

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi160K → R: No association with APAF-1. No binding to pro-caspase-9. 1 Publication1
Mutagenesisi368M → L: Activation of pro-caspase-9 independent of cytochrome c. Increased ability to induce apoptosis. 1 Publication1

Organism-specific databases

DisGeNETi317
OpenTargetsiENSG00000120868
PharmGKBiPA24868

Chemistry databases

ChEMBLiCHEMBL1795093
DrugBankiDB00171 Adenosine triphosphate

Polymorphism and mutation databases

BioMutaiAPAF1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508441 – 1248Apoptotic protease-activating factor 1Add BLAST1248

Proteomic databases

EPDiO14727
MaxQBiO14727
PaxDbiO14727
PeptideAtlasiO14727
PRIDEiO14727
ProteomicsDBi48182
48183 [O14727-2]
48184 [O14727-3]
48185 [O14727-4]
48186 [O14727-5]
48187 [O14727-6]

PTM databases

iPTMnetiO14727
PhosphoSitePlusiO14727

Miscellaneous databases

PMAP-CutDBiO14727

Expressioni

Tissue specificityi

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Inductioni

By E2F and p53/TP53 in apoptotic neurons.1 Publication

Gene expression databases

BgeeiENSG00000120868 Expressed in 196 organ(s), highest expression level in caudate nucleus
ExpressionAtlasiO14727 baseline and differential
GenevisibleiO14727 HS

Organism-specific databases

HPAiCAB069399
HPA031373

Interactioni

Subunit structurei

Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106814, 30 interactors
CORUMiO14727
DIPiDIP-27624N
IntActiO14727, 7 interactors
MINTiO14727
STRINGi9606.ENSP00000448165

Chemistry databases

BindingDBiO14727

Structurei

Secondary structure

11248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO14727
SMRiO14727
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14727

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90CARDPROSITE-ProRule annotationAdd BLAST90
Domaini104 – 415NB-ARCAdd BLAST312
Repeati613 – 652WD 1-1Add BLAST40
Repeati655 – 694WD 1-2Add BLAST40
Repeati697 – 738WD 1-3Add BLAST42
Repeati741 – 780WD 1-4Add BLAST40
Repeati796 – 836WD 1-5Add BLAST41
Repeati838 – 877WD 1-6Add BLAST40
Repeati880 – 910WD 1-7Add BLAST31
Repeati922 – 958WD 2-1Add BLAST37
Repeati959 – 998WD 2-2Add BLAST40
Repeati1001 – 1040WD 2-3Add BLAST40
Repeati1042 – 1080WD 2-4Add BLAST39
Repeati1083 – 1122WD 2-5Add BLAST40
Repeati1125 – 1164WD 2-6Add BLAST40
Repeati1175 – 1212WD 2-7Add BLAST38
Repeati1213 – 1248WD 2-8Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni910 – 921Interpropeller linkerBy similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi95 – 98Poly-Ser4

Domaini

The CARD domain mediates interaction with APIP.
The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization (By similarity).By similarity

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
KOG4658 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOVERGENiHBG018730
InParanoidiO14727
KOiK02084
OMAiSRIGHKK
OrthoDBiEOG091G036U
PhylomeDBiO14727
TreeFamiTF323866

Family and domain databases

CDDicd08323 CARD_APAF1, 1 hit
Gene3Di1.10.10.10, 1 hit
2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR017251 Apaf-1
IPR037963 APAF1_CARD_dom
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
IPR020472 G-protein_beta_WD-40_rep
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00619 CARD, 1 hit
PF00931 NB-ARC, 1 hit
PF00400 WD40, 9 hits
PIRSFiPIRSF037646 Apop_pept_activating-1, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 13 hits
SUPFAMiSSF47986 SSF47986, 1 hit
SSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 9 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O14727-1) [UniParc]FASTAAdd to basket
Also known as: Apaf-1XL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ
60 70 80 90 100
QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK
110 120 130 140 150
DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV
160 170 180 190 200
TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ
210 220 230 240 250
NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV
260 270 280 290 300
LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
310 320 330 340 350
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF
360 370 380 390 400
KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL
410 420 430 440 450
CILWDMETEE VEDILQEFVN KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC
460 470 480 490 500
SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC MYWYNFLAYH MASAKMHKEL
510 520 530 540 550
CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV SENFQEFLSL
560 570 580 590 600
NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
610 620 630 640 650
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL
660 670 680 690 700
LEIKAHEDEV LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE
710 720 730 740 750
QVNCCHFTNS SHHLLLATGS SDCFLKLWDL NQKECRNTMF GHTNSVNHCR
760 770 780 790 800
FSPDDKLLAS CSADGTLKLW DATSANERKS INVKQFFLNL EDPQEDMEVI
810 820 830 840 850
VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH STIQYCDFSP
860 870 880 890 900
QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
910 920 930 940 950
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN
960 970 980 990 1000
GRTGQIDYLT EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF
1010 1020 1030 1040 1050
QHKKTVWHIQ FTADEKTLIS SSDDAEIQVW NWQLDKCIFL RGHQETVKDF
1060 1070 1080 1090 1100
RLLKNSRLLS WSFDGTVKVW NIITGNKEKD FVCHQGTVLS CDISHDATKF
1110 1120 1130 1140 1150
SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST LLATGDDNGE
1160 1170 1180 1190 1200
IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
1210 1220 1230 1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE
Length:1,248
Mass (Da):141,840
Last modified:January 23, 2002 - v2
Checksum:i0750D05817AC9B3B
GO
Isoform 2 (identifier: O14727-2) [UniParc]FASTAAdd to basket
Also known as: Apaf-1L

The sequence of this isoform differs from the canonical sequence as follows:
     99-109: Missing.

Show »
Length:1,237
Mass (Da):140,745
Checksum:i0D3EF9AB2A66FFF5
GO
Isoform 3 (identifier: O14727-3) [UniParc]FASTAAdd to basket
Also known as: Apaf-1S

The sequence of this isoform differs from the canonical sequence as follows:
     99-109: Missing.
     824-866: Missing.

Show »
Length:1,194
Mass (Da):135,980
Checksum:iA675EA102DDAAFB7
GO
Isoform 4 (identifier: O14727-4) [UniParc]FASTAAdd to basket
Also known as: Apaf-1M

The sequence of this isoform differs from the canonical sequence as follows:
     824-866: Missing.

Show »
Length:1,205
Mass (Da):137,076
Checksum:iDA57B4E35E79D73A
GO
Isoform 5 (identifier: O14727-5) [UniParc]FASTAAdd to basket
Also known as: Apaf-1XS

The sequence of this isoform differs from the canonical sequence as follows:
     575-575: E → ETLGFESKK
     824-866: Missing.
     1113-1154: Missing.

Show »
Length:1,171
Mass (Da):133,356
Checksum:iCA9B14019EAB35BF
GO
Isoform 6 (identifier: O14727-6) [UniParc]FASTAAdd to basket
Also known as: Apaf-1-ALT

The sequence of this isoform differs from the canonical sequence as follows:
     319-338: GSPLVVSLIGALLRDFPNRW → VVERCHWGILTDLLHKWNQS
     339-1248: Missing.

Show »
Length:338
Mass (Da):37,976
Checksum:i29D933A65707ED92
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JLV4C9JLV4_HUMAN
Apoptotic protease-activating facto...
APAF1
1,163Annotation score:
F8VNZ0F8VNZ0_HUMAN
Apoptotic protease-activating facto...
APAF1
130Annotation score:

Sequence cautioni

The sequence AAK28401 differs from that shown. Reason: Frameshift at position 108.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134N → S (Ref. 11) Curated1
Sequence conflicti145G → C in CAB55587 (PubMed:10441496).Curated1
Sequence conflicti161S → F in CAB55586 (PubMed:10441496).Curated1
Sequence conflicti370I → T in CAB55581 (PubMed:10441496).Curated1
Sequence conflicti383Y → H in CAB55586 (PubMed:10441496).Curated1
Sequence conflicti544F → L in CAB55584 (PubMed:10441496).Curated1
Sequence conflicti580A → T in CAB55580 (PubMed:10441496).Curated1
Sequence conflicti608R → C in CAB55585 (PubMed:10441496).Curated1
Sequence conflicti620H → R in CAB55587 (PubMed:10441496).Curated1
Sequence conflicti639L → F in CAB55583 (PubMed:10441496).Curated1
Sequence conflicti708T → A in CAB55579 (PubMed:10441496).Curated1
Sequence conflicti742H → R in CAB55584 (PubMed:10441496).Curated1
Sequence conflicti746V → A in CAB55586 (PubMed:10441496).Curated1
Sequence conflicti757L → P in CAB56462 (PubMed:10441496).Curated1
Sequence conflicti795E → G in CAB55581 (PubMed:10441496).Curated1
Sequence conflicti798E → G in CAB55587 (PubMed:10441496).Curated1
Sequence conflicti825D → A in CAB55585 (PubMed:10441496).Curated1
Sequence conflicti871S → L in CAB55587 (PubMed:10441496).Curated1
Sequence conflicti876A → T in CAB55581 (PubMed:10441496).Curated1
Sequence conflicti949I → V in CAB55585 (PubMed:10441496).Curated1
Sequence conflicti1008H → R in CAB55582 (PubMed:10441496).Curated1
Sequence conflicti1056S → P in CAB55582 (PubMed:10441496).Curated1
Sequence conflicti1241L → I in BAA24843 (PubMed:9455477).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00675999 – 109Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST11
Alternative sequenceiVSP_008965319 – 338GSPLV…FPNRW → VVERCHWGILTDLLHKWNQS in isoform 6. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_008966339 – 1248Missing in isoform 6. 1 PublicationAdd BLAST910
Alternative sequenceiVSP_006760575E → ETLGFESKK in isoform 5. 1 Publication1
Alternative sequenceiVSP_006761824 – 866Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST43
Alternative sequenceiVSP_0067621113 – 1154Missing in isoform 5. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013263 mRNA Translation: AAC51678.1
AJ243003 mRNA Translation: CAB55579.1
AJ243004 mRNA Translation: CAB55580.1
AJ243005 mRNA Translation: CAB55581.1
AJ243006 mRNA Translation: CAB55582.1
AJ243007 mRNA Translation: CAB55583.1
AJ243008 mRNA Translation: CAB55584.1
AJ243009 mRNA Translation: CAB55585.1
AJ243010 mRNA Translation: CAB55586.1
AJ243011 mRNA Translation: CAB55587.1
AJ243048 mRNA Translation: CAB55588.1
AJ243107 mRNA Translation: CAB56462.1
AF134397 mRNA Translation: AAD38344.1
AF149794 mRNA Translation: AAD34016.1
AB007873 mRNA Translation: BAA24843.2
AB103079 mRNA Translation: BAC77343.1
CH471054 Genomic DNA Translation: EAW97606.1
BC136531 mRNA Translation: AAI36532.1
BC136532 mRNA Translation: AAI36533.1
AJ133643 Genomic DNA Translation: CAB65085.1
AJ133644 Genomic DNA Translation: CAB65086.1
AJ133645 Genomic DNA Translation: CAB65087.1
AF248734 mRNA Translation: AAK28401.1 Frameshift.
CCDSiCCDS55862.1 [O14727-2]
CCDS55863.1 [O14727-3]
CCDS9069.1 [O14727-1]
CCDS9070.1 [O14727-4]
CCDS9071.1 [O14727-6]
PIRiT03818
RefSeqiNP_001151.1, NM_001160.2 [O14727-3]
NP_037361.1, NM_013229.2 [O14727-2]
NP_863651.1, NM_181861.1 [O14727-1]
NP_863658.1, NM_181868.1 [O14727-4]
NP_863659.1, NM_181869.1 [O14727-6]
UniGeneiHs.552567

Genome annotation databases

EnsembliENST00000333991; ENSP00000334558; ENSG00000120868 [O14727-6]
ENST00000357310; ENSP00000349862; ENSG00000120868 [O14727-4]
ENST00000359972; ENSP00000353059; ENSG00000120868 [O14727-3]
ENST00000547045; ENSP00000449791; ENSG00000120868 [O14727-4]
ENST00000550527; ENSP00000448449; ENSG00000120868 [O14727-2]
ENST00000551964; ENSP00000448165; ENSG00000120868 [O14727-1]
ENST00000552268; ENSP00000448826; ENSG00000120868 [O14727-6]
GeneIDi317
KEGGihsa:317
UCSCiuc001tfz.4 human [O14727-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013263 mRNA Translation: AAC51678.1
AJ243003 mRNA Translation: CAB55579.1
AJ243004 mRNA Translation: CAB55580.1
AJ243005 mRNA Translation: CAB55581.1
AJ243006 mRNA Translation: CAB55582.1
AJ243007 mRNA Translation: CAB55583.1
AJ243008 mRNA Translation: CAB55584.1
AJ243009 mRNA Translation: CAB55585.1
AJ243010 mRNA Translation: CAB55586.1
AJ243011 mRNA Translation: CAB55587.1
AJ243048 mRNA Translation: CAB55588.1
AJ243107 mRNA Translation: CAB56462.1
AF134397 mRNA Translation: AAD38344.1
AF149794 mRNA Translation: AAD34016.1
AB007873 mRNA Translation: BAA24843.2
AB103079 mRNA Translation: BAC77343.1
CH471054 Genomic DNA Translation: EAW97606.1
BC136531 mRNA Translation: AAI36532.1
BC136532 mRNA Translation: AAI36533.1
AJ133643 Genomic DNA Translation: CAB65085.1
AJ133644 Genomic DNA Translation: CAB65086.1
AJ133645 Genomic DNA Translation: CAB65087.1
AF248734 mRNA Translation: AAK28401.1 Frameshift.
CCDSiCCDS55862.1 [O14727-2]
CCDS55863.1 [O14727-3]
CCDS9069.1 [O14727-1]
CCDS9070.1 [O14727-4]
CCDS9071.1 [O14727-6]
PIRiT03818
RefSeqiNP_001151.1, NM_001160.2 [O14727-3]
NP_037361.1, NM_013229.2 [O14727-2]
NP_863651.1, NM_181861.1 [O14727-1]
NP_863658.1, NM_181868.1 [O14727-4]
NP_863659.1, NM_181869.1 [O14727-6]
UniGeneiHs.552567

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C15NMR-A1-97[»]
1CWWNMR-A1-97[»]
1CY5X-ray1.30A1-97[»]
1Z6TX-ray2.21A/B/C/D1-591[»]
2P1HX-ray1.59A1-92[»]
2YGSX-ray1.60A1-92[»]
3J2Telectron microscopy9.5A/B/C/D/E/F/G1-1248[»]
3JBTelectron microscopy3.80A/C/E/G/I/K/M1-1248[»]
3YGSX-ray2.50C1-95[»]
4RHWX-ray2.10A/B/C/D1-97[»]
5JUYelectron microscopy4.10A/B/C/D/E/F/G1-1248[»]
5WVCX-ray2.99A/C/E1-95[»]
5WVEelectron microscopy4.40A/C/E/G/I/K/M1-1248[»]
O/P/Q/R/W/X1-102[»]
ProteinModelPortaliO14727
SMRiO14727
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106814, 30 interactors
CORUMiO14727
DIPiDIP-27624N
IntActiO14727, 7 interactors
MINTiO14727
STRINGi9606.ENSP00000448165

Chemistry databases

BindingDBiO14727
ChEMBLiCHEMBL1795093
DrugBankiDB00171 Adenosine triphosphate

PTM databases

iPTMnetiO14727
PhosphoSitePlusiO14727

Polymorphism and mutation databases

BioMutaiAPAF1

Proteomic databases

EPDiO14727
MaxQBiO14727
PaxDbiO14727
PeptideAtlasiO14727
PRIDEiO14727
ProteomicsDBi48182
48183 [O14727-2]
48184 [O14727-3]
48185 [O14727-4]
48186 [O14727-5]
48187 [O14727-6]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333991; ENSP00000334558; ENSG00000120868 [O14727-6]
ENST00000357310; ENSP00000349862; ENSG00000120868 [O14727-4]
ENST00000359972; ENSP00000353059; ENSG00000120868 [O14727-3]
ENST00000547045; ENSP00000449791; ENSG00000120868 [O14727-4]
ENST00000550527; ENSP00000448449; ENSG00000120868 [O14727-2]
ENST00000551964; ENSP00000448165; ENSG00000120868 [O14727-1]
ENST00000552268; ENSP00000448826; ENSG00000120868 [O14727-6]
GeneIDi317
KEGGihsa:317
UCSCiuc001tfz.4 human [O14727-1]

Organism-specific databases

CTDi317
DisGeNETi317
EuPathDBiHostDB:ENSG00000120868.13
GeneCardsiAPAF1
HGNCiHGNC:576 APAF1
HPAiCAB069399
HPA031373
MIMi602233 gene
neXtProtiNX_O14727
OpenTargetsiENSG00000120868
PharmGKBiPA24868
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
KOG4658 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOVERGENiHBG018730
InParanoidiO14727
KOiK02084
OMAiSRIGHKK
OrthoDBiEOG091G036U
PhylomeDBiO14727
TreeFamiTF323866

Enzyme and pathway databases

ReactomeiR-HSA-111458 Formation of apoptosome
R-HSA-111459 Activation of caspases through apoptosome-mediated cleavage
R-HSA-6798695 Neutrophil degranulation
R-HSA-6803207 TP53 Regulates Transcription of Caspase Activators and Caspases
R-HSA-8953750 Transcriptional Regulation by E2F6
SABIO-RKiO14727
SignaLinkiO14727
SIGNORiO14727

Miscellaneous databases

EvolutionaryTraceiO14727
GeneWikiiAPAF1
GenomeRNAii317
PMAP-CutDBiO14727
PROiPR:O14727
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120868 Expressed in 196 organ(s), highest expression level in caudate nucleus
ExpressionAtlasiO14727 baseline and differential
GenevisibleiO14727 HS

Family and domain databases

CDDicd08323 CARD_APAF1, 1 hit
Gene3Di1.10.10.10, 1 hit
2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR017251 Apaf-1
IPR037963 APAF1_CARD_dom
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
IPR020472 G-protein_beta_WD-40_rep
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00619 CARD, 1 hit
PF00931 NB-ARC, 1 hit
PF00400 WD40, 9 hits
PIRSFiPIRSF037646 Apop_pept_activating-1, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 13 hits
SUPFAMiSSF47986 SSF47986, 1 hit
SSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 9 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAPAF_HUMAN
AccessioniPrimary (citable) accession number: O14727
Secondary accession number(s): B2RMX8
, O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9, Q9UGP0, Q9UJ58, Q9UJ59, Q9UJ60, Q9UJ61, Q9UJ62, Q9UJ63, Q9UJ64, Q9UJ65, Q9UJ66, Q9UJ67, Q9UNC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: November 7, 2018
This is version 212 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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