Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Gene

CTDSP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per monomer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1074-aspartylphosphate intermediateBy similarity1
Metal bindingi107Magnesium1 Publication1
Active sitei109Proton donorBy similarity1
Metal bindingi109Magnesium; via carbonyl oxygen1 Publication1
Sitei163Transition state stabilizerBy similarity1
Sitei201Transition state stabilizerBy similarity1
Metal bindingi218Magnesium1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: GO_Central
  • RNA polymerase II CTD heptapeptide repeat phosphatase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-HSA-381038 XBP1(S) activates chaperone genes
SignaLinkiO14595
SIGNORiO14595

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 (EC:3.1.3.16)
Alternative name(s):
Nuclear LIM interactor-interacting factor 2
Short name:
NLI-interacting factor 2
Protein OS-4
Small C-terminal domain phosphatase 2
Small CTD phosphatase 2
Short name:
SCP2
Gene namesi
Name:CTDSP2
Synonyms:NIF2, OS4, SCP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000175215.9
HGNCiHGNC:17077 CTDSP2
MIMi608711 gene
neXtProtiNX_O14595

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10106
OpenTargetsiENSG00000175215
PharmGKBiPA128394568

Polymorphism and mutation databases

BioMutaiCTDSP2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125741 – 271Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14595
PaxDbiO14595
PeptideAtlasiO14595
PRIDEiO14595
ProteomicsDBi48104

PTM databases

DEPODiO14595
iPTMnetiO14595
PhosphoSitePlusiO14595
SwissPalmiO14595

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues. Highest expression in pancreas and lowest in liver.1 Publication

Inductioni

In primary sarcomas.

Gene expression databases

BgeeiENSG00000175215 Expressed in 242 organ(s), highest expression level in urinary bladder
CleanExiHS_CTDSP2
HS_SCP2
ExpressionAtlasiO14595 baseline and differential
GenevisibleiO14595 HS

Organism-specific databases

HPAiHPA052607

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with REST.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115412, 39 interactors
DIPiDIP-61245N
IntActiO14595, 11 interactors
MINTiO14595
STRINGi9606.ENSP00000381148

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO14595
SMRiO14595
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14595

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 255FCP1 homologyPROSITE-ProRule annotationAdd BLAST159

Phylogenomic databases

eggNOGiKOG1605 Eukaryota
COG5190 LUCA
GeneTreeiENSGT00390000017194
HOGENOMiHOG000236379
HOVERGENiHBG053298
InParanoidiO14595
KOiK15731
OMAiANTIAKX
PhylomeDBiO14595
TreeFamiTF313556

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR011948 Dullard_phosphatase
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR040078 RNA_Pol_CTD_Phosphatase
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SFLDiSFLDG01124 C0.1:_RNA_Pol_CTD_Phosphatase_, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02251 HIF-SF_euk, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O14595-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEHGSIITQA RREDALVLTK QGLVSKSSPK KPRGRNIFKA LFCCFRAQHV
60 70 80 90 100
GQSSSSTELA AYKEEANTIA KSDLLQCLQY QFYQIPGTCL LPEVTEEDQG
110 120 130 140 150
RICVVIDLDE TLVHSSFKPI NNADFIVPIE IEGTTHQVYV LKRPYVDEFL
160 170 180 190 200
RRMGELFECV LFTASLAKYA DPVTDLLDRC GVFRARLFRE SCVFHQGCYV
210 220 230 240 250
KDLSRLGRDL RKTLILDNSP ASYIFHPENA VPVQSWFDDM ADTELLNLIP
260 270
IFEELSGAED VYTSLGQLRA P
Length:271
Mass (Da):30,664
Last modified:November 9, 2004 - v2
Checksum:iE4FA4A1657F2B03F
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YI12H0YI12_HUMAN
Carboxy-terminal domain RNA polymer...
CTDSP2
182Annotation score:
F8W184F8W184_HUMAN
Carboxy-terminal domain RNA polymer...
CTDSP2
119Annotation score:
F8W1I1F8W1I1_HUMAN
Carboxy-terminal domain RNA polymer...
CTDSP2
98Annotation score:
H0YI29H0YI29_HUMAN
Carboxy-terminal domain RNA polymer...
CTDSP2
125Annotation score:
F8VYL0F8VYL0_HUMAN
Carboxy-terminal domain RNA polymer...
CTDSP2
102Annotation score:

Sequence cautioni

The sequence AAB71816 differs from that shown. Reason: Frameshift at position 267.Curated
The sequence AAP34399 differs from that shown. Reason: Frameshift at position 267.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9Q → H in AAD09331 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000152 mRNA Translation: AAB71816.1 Frameshift.
AY279531 mRNA Translation: AAP34399.1 Frameshift.
AF022231 mRNA Translation: AAD09331.1
AK291289 mRNA Translation: BAF83978.1
CH471054 Genomic DNA Translation: EAW97083.1
BC065920 mRNA Translation: AAH65920.1
CCDSiCCDS41801.1
RefSeqiNP_005721.3, NM_005730.3
UniGeneiHs.524530

Genome annotation databases

EnsembliENST00000398073; ENSP00000381148; ENSG00000175215
GeneIDi10106
KEGGihsa:10106
UCSCiuc001sqm.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000152 mRNA Translation: AAB71816.1 Frameshift.
AY279531 mRNA Translation: AAP34399.1 Frameshift.
AF022231 mRNA Translation: AAD09331.1
AK291289 mRNA Translation: BAF83978.1
CH471054 Genomic DNA Translation: EAW97083.1
BC065920 mRNA Translation: AAH65920.1
CCDSiCCDS41801.1
RefSeqiNP_005721.3, NM_005730.3
UniGeneiHs.524530

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q5EX-ray2.51A/B/C/D/E/F/G/H87-271[»]
ProteinModelPortaliO14595
SMRiO14595
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115412, 39 interactors
DIPiDIP-61245N
IntActiO14595, 11 interactors
MINTiO14595
STRINGi9606.ENSP00000381148

PTM databases

DEPODiO14595
iPTMnetiO14595
PhosphoSitePlusiO14595
SwissPalmiO14595

Polymorphism and mutation databases

BioMutaiCTDSP2

Proteomic databases

MaxQBiO14595
PaxDbiO14595
PeptideAtlasiO14595
PRIDEiO14595
ProteomicsDBi48104

Protocols and materials databases

DNASUi10106
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398073; ENSP00000381148; ENSG00000175215
GeneIDi10106
KEGGihsa:10106
UCSCiuc001sqm.4 human

Organism-specific databases

CTDi10106
DisGeNETi10106
EuPathDBiHostDB:ENSG00000175215.9
GeneCardsiCTDSP2
HGNCiHGNC:17077 CTDSP2
HPAiHPA052607
MIMi608711 gene
neXtProtiNX_O14595
OpenTargetsiENSG00000175215
PharmGKBiPA128394568
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1605 Eukaryota
COG5190 LUCA
GeneTreeiENSGT00390000017194
HOGENOMiHOG000236379
HOVERGENiHBG053298
InParanoidiO14595
KOiK15731
OMAiANTIAKX
PhylomeDBiO14595
TreeFamiTF313556

Enzyme and pathway databases

ReactomeiR-HSA-381038 XBP1(S) activates chaperone genes
SignaLinkiO14595
SIGNORiO14595

Miscellaneous databases

ChiTaRSiCTDSP2 human
EvolutionaryTraceiO14595
GeneWikiiCTDSP2
GenomeRNAii10106
PROiPR:O14595
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175215 Expressed in 242 organ(s), highest expression level in urinary bladder
CleanExiHS_CTDSP2
HS_SCP2
ExpressionAtlasiO14595 baseline and differential
GenevisibleiO14595 HS

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR011948 Dullard_phosphatase
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR040078 RNA_Pol_CTD_Phosphatase
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SFLDiSFLDG01124 C0.1:_RNA_Pol_CTD_Phosphatase_, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02251 HIF-SF_euk, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTDS2_HUMAN
AccessioniPrimary (citable) accession number: O14595
Secondary accession number(s): A8K5H4
, Q53ZR2, Q6NZY3, Q9UEX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: November 9, 2004
Last modified: November 7, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again