UniProtKB - O14524 (NEMP1_HUMAN)
Protein
Nuclear envelope integral membrane protein 1
Gene
NEMP1
Organism
Homo sapiens (Human)
Status
Functioni
GO - Biological processi
- nuclear membrane organization Source: FlyBase
Enzyme and pathway databases
PathwayCommonsi | O14524 |
Protein family/group databases
TCDBi | 9.B.368.1.1, the tmem194a (tmem194a) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear envelope integral membrane protein 1 |
Gene namesi | Name:NEMP1 Synonyms:KIAA0286, TMEM194, TMEM194A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29001, NEMP1 |
MIMi | 616496, gene |
neXtProti | NX_O14524 |
VEuPathDBi | HostDB:ENSG00000166881.9 |
Subcellular locationi
Nucleus
- Nucleus inner membrane By similarity; Multi-pass membrane protein Sequence analysis; Nucleoplasmic side By similarity
- Nucleus envelope By similarity
Note: Colocalizes with lamins and RAN-GTP at the nuclear envelope.By similarity
Nucleus
- nuclear envelope Source: UniProtKB
- nuclear inner membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 161 – 181 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 186 – 206 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 216 – 236 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 245 – 265 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 289 – 309 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 23306 |
OpenTargetsi | ENSG00000166881 |
PharmGKBi | PA162406290 |
Miscellaneous databases
Pharosi | O14524, Tdark |
Genetic variation databases
BioMutai | NEMP1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 43 | Sequence analysisAdd BLAST | 43 | |
ChainiPRO_0000050744 | 44 – 444 | Nuclear envelope integral membrane protein 1Add BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 125 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 368 | PhosphoserineCombined sources | 1 | |
Modified residuei | 424 | PhosphoserineCombined sources | 1 | |
Modified residuei | 425 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylation may regulate its interaction with RAN-GTP.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | O14524 |
jPOSTi | O14524 |
MassIVEi | O14524 |
MaxQBi | O14524 |
PaxDbi | O14524 |
PeptideAtlasi | O14524 |
PRIDEi | O14524 |
ProteomicsDBi | 48069 [O14524-1] 48070 [O14524-2] |
PTM databases
GlyConnecti | 1577, 1 N-Linked glycan (1 site) |
GlyGeni | O14524, 1 site |
iPTMneti | O14524 |
PhosphoSitePlusi | O14524 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166881, Expressed in female gonad and 194 other tissues |
ExpressionAtlasi | O14524, baseline and differential |
Genevisiblei | O14524, HS |
Organism-specific databases
HPAi | ENSG00000166881, Low tissue specificity |
Interactioni
Subunit structurei
Homooligomer.
Interacts with RAN-GTP.
By similarityBinary interactionsi
Hide detailsIsoform 2 [O14524-2]
Protein-protein interaction databases
BioGRIDi | 116898, 72 interactors |
IntActi | O14524, 56 interactors |
MINTi | O14524 |
STRINGi | 9606.ENSP00000300128 |
Miscellaneous databases
RNActi | O14524, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 186 – 297 | A; required for its colocalization with lamins at the nuclear envelopeBy similarityAdd BLAST | 112 | |
Regioni | 336 – 444 | Required for nuclear localizationBy similarityAdd BLAST | 109 | |
Regioni | 336 – 405 | B; required for interaction with RAN-GTPBy similarityAdd BLAST | 70 |
Domaini
The transmembrane domains are required and sufficient for its oligomerization.By similarity
Sequence similaritiesi
Belongs to the NEMP family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3817, Eukaryota |
GeneTreei | ENSGT00390000002174 |
HOGENOMi | CLU_025225_0_0_1 |
InParanoidi | O14524 |
OMAi | INILSWT |
OrthoDBi | 536946at2759 |
PhylomeDBi | O14524 |
TreeFami | TF314831 |
Family and domain databases
InterProi | View protein in InterPro IPR019358, NEMP_fam |
PANTHERi | PTHR13598, PTHR13598, 1 hit |
Pfami | View protein in Pfam PF10225, NEMP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O14524-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGGMKVAVS PAVGPGPWGS GVGGGGTVRL LLILSGCLVY GTAETDVNVV
60 70 80 90 100
MLQESQVCEK RASQQFCYTN VLIPKWHDIW TRIQIRVNSS RLVRVTQVEN
110 120 130 140 150
EEKLKELEQF SIWNFFSSFL KEKLNDTYVN VGLYSTKTCL KVEIIEKDTK
160 170 180 190 200
YSVIVIRRFD PKLFLVFLLG LMLFFCGDLL SRSQIFYYST GMTVGIVASL
210 220 230 240 250
LIIIFILSKF MPKKSPIYVI LVGGWSFSLY LIQLVFKNLQ EIWRCYWQYL
260 270 280 290 300
LSYVLTVGFM SFAVCYKYGP LENERSINLL TWTLQLMGLC FMYSGIQIPH
310 320 330 340 350
IALAIIIIAL CTKNLEHPIQ WLYITCRKVC KGAEKPVPPR LLTEEEYRIQ
360 370 380 390 400
GEVETRKALE ELREFCNSPD CSAWKTVSRI QSPKRFADFV EGSSHLTPNE
410 420 430 440
VSVHEQEYGL GSIIAQDEIY EEASSEEEDS YSRCPAITQN NFLT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V5K2 | G3V5K2_HUMAN | Nuclear envelope integral membrane ... | NEMP1 | 110 | Annotation score: |
Sequence cautioni
The sequence AAH71597 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA22955 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 442 | F → L in CAH18250 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057817 | 217 | I → V. Corresponds to variant dbSNP:rs17546579Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011896 | 110 – 182 | Missing in isoform 2. 3 PublicationsAdd BLAST | 73 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006624 mRNA Translation: BAA22955.2 Different initiation. CR749406 mRNA Translation: CAH18250.1 AK294566 mRNA Translation: BAG57761.1 BC071597 mRNA Translation: AAH71597.1 Different initiation. BC117436 mRNA Translation: AAI17437.1 BC126300 mRNA Translation: AAI26301.1 |
CCDSi | CCDS31841.1 [O14524-2] CCDS44927.1 [O14524-1] |
RefSeqi | NP_001124435.1, NM_001130963.1 [O14524-1] NP_056072.2, NM_015257.2 [O14524-2] |
Genome annotation databases
Ensembli | ENST00000300128; ENSP00000300128; ENSG00000166881 [O14524-1] ENST00000379391; ENSP00000368701; ENSG00000166881 [O14524-2] |
GeneIDi | 23306 |
KEGGi | hsa:23306 |
UCSCi | uc001smx.4, human [O14524-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006624 mRNA Translation: BAA22955.2 Different initiation. CR749406 mRNA Translation: CAH18250.1 AK294566 mRNA Translation: BAG57761.1 BC071597 mRNA Translation: AAH71597.1 Different initiation. BC117436 mRNA Translation: AAI17437.1 BC126300 mRNA Translation: AAI26301.1 |
CCDSi | CCDS31841.1 [O14524-2] CCDS44927.1 [O14524-1] |
RefSeqi | NP_001124435.1, NM_001130963.1 [O14524-1] NP_056072.2, NM_015257.2 [O14524-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 116898, 72 interactors |
IntActi | O14524, 56 interactors |
MINTi | O14524 |
STRINGi | 9606.ENSP00000300128 |
Protein family/group databases
TCDBi | 9.B.368.1.1, the tmem194a (tmem194a) family |
PTM databases
GlyConnecti | 1577, 1 N-Linked glycan (1 site) |
GlyGeni | O14524, 1 site |
iPTMneti | O14524 |
PhosphoSitePlusi | O14524 |
Genetic variation databases
BioMutai | NEMP1 |
Proteomic databases
EPDi | O14524 |
jPOSTi | O14524 |
MassIVEi | O14524 |
MaxQBi | O14524 |
PaxDbi | O14524 |
PeptideAtlasi | O14524 |
PRIDEi | O14524 |
ProteomicsDBi | 48069 [O14524-1] 48070 [O14524-2] |
Protocols and materials databases
Antibodypediai | 16059, 26 antibodies |
DNASUi | 23306 |
Genome annotation databases
Ensembli | ENST00000300128; ENSP00000300128; ENSG00000166881 [O14524-1] ENST00000379391; ENSP00000368701; ENSG00000166881 [O14524-2] |
GeneIDi | 23306 |
KEGGi | hsa:23306 |
UCSCi | uc001smx.4, human [O14524-1] |
Organism-specific databases
CTDi | 23306 |
DisGeNETi | 23306 |
GeneCardsi | NEMP1 |
HGNCi | HGNC:29001, NEMP1 |
HPAi | ENSG00000166881, Low tissue specificity |
MIMi | 616496, gene |
neXtProti | NX_O14524 |
OpenTargetsi | ENSG00000166881 |
PharmGKBi | PA162406290 |
VEuPathDBi | HostDB:ENSG00000166881.9 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3817, Eukaryota |
GeneTreei | ENSGT00390000002174 |
HOGENOMi | CLU_025225_0_0_1 |
InParanoidi | O14524 |
OMAi | INILSWT |
OrthoDBi | 536946at2759 |
PhylomeDBi | O14524 |
TreeFami | TF314831 |
Enzyme and pathway databases
PathwayCommonsi | O14524 |
Miscellaneous databases
BioGRID-ORCSi | 23306, 7 hits in 877 CRISPR screens |
ChiTaRSi | NEMP1, human |
GenomeRNAii | 23306 |
Pharosi | O14524, Tdark |
PROi | PR:O14524 |
RNActi | O14524, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166881, Expressed in female gonad and 194 other tissues |
ExpressionAtlasi | O14524, baseline and differential |
Genevisiblei | O14524, HS |
Family and domain databases
InterProi | View protein in InterPro IPR019358, NEMP_fam |
PANTHERi | PTHR13598, PTHR13598, 1 hit |
Pfami | View protein in Pfam PF10225, NEMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEMP1_HUMAN | |
Accessioni | O14524Primary (citable) accession number: O14524 Secondary accession number(s): Q17R72, Q68DH0, Q6IQ25 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants