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Protein

Phospholipid phosphatase 3

Gene

PLPP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions.

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

Activity regulationi

Inhibited by sphingosine, zinc ions and propanolol. Not inhibited by N-ethylmaleimide treatment.

GO - Molecular functioni

  • lipid phosphatase activity Source: GO_Central
  • phosphatase activity Source: GO_Central
  • phosphatidate phosphatase activity Source: GO_Central
  • phosphoprotein phosphatase activity Source: ProtInc
  • sphingosine-1-phosphate phosphatase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-HSA-1660661 Sphingolipid de novo biosynthesis
SignaLinkiO14495

Chemistry databases

SwissLipidsiSLP:000001642

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid phosphatase 3Imported (EC:3.1.3.4)
Alternative name(s):
Lipid phosphate phosphohydrolase 3
PAP2-beta
Phosphatidate phosphohydrolase type 2b
Phosphatidic acid phosphatase 2b
Short name:
PAP-2b
Short name:
PAP2b
Vascular endothelial growth factor and type I collagen-inducible protein
Short name:
VCIP
Gene namesi
Name:PLPP3Imported
Synonyms:LPP3, PPAP2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162407.8
HGNCiHGNC:9229 PLPP3
MIMi607125 gene
neXtProtiNX_O14495

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 85LumenalSequence analysisAdd BLAST31
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Topological domaini107 – 122CytoplasmicSequence analysisAdd BLAST16
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 193LumenalSequence analysisAdd BLAST50
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215 – 227CytoplasmicSequence analysisAdd BLAST13
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 257LumenalSequence analysis9
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279 – 311CytoplasmicSequence analysisAdd BLAST33

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8613
OpenTargetsiENSG00000162407
PharmGKBiPA33553

Polymorphism and mutation databases

BioMutaiPPAP2B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002209121 – 311Phospholipid phosphatase 3Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated. Contains high-mannose oligosaccharides.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO14495
MaxQBiO14495
PaxDbiO14495
PeptideAtlasiO14495
PRIDEiO14495
ProteomicsDBi48039

PTM databases

DEPODiO14495
iPTMnetiO14495
PhosphoSitePlusiO14495

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in heart and placenta.

Inductioni

By EGF, VEGF, FGF2 and phorbol myristate acetate (PMA).1 Publication

Gene expression databases

BgeeiENSG00000162407 Expressed in 239 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_PPAP2B
GenevisibleiO14495 HS

Organism-specific databases

HPAiHPA028892
HPA072751

Interactioni

Subunit structurei

Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTNND1O607169EBI-766232,EBI-701927

Protein-protein interaction databases

BioGridi114171, 9 interactors
CORUMiO14495
IntActiO14495, 4 interactors
MINTiO14495
STRINGi9606.ENSP00000360296

Structurei

3D structure databases

ProteinModelPortaliO14495
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3030 Eukaryota
COG0671 LUCA
GeneTreeiENSGT00620000087654
HOGENOMiHOG000041307
HOVERGENiHBG002048
InParanoidiO14495
KOiK01080
OMAiQNYKCRG
OrthoDBiEOG091G0K5H
PhylomeDBiO14495
TreeFamiTF316040

Family and domain databases

InterProiView protein in InterPro
IPR028675 LPP3
IPR036938 P_Acid_Pase_2/haloperoxi_sf
IPR000326 P_Acid_Pase_2/haloperoxidase
PANTHERiPTHR10165:SF79 PTHR10165:SF79, 1 hit
PfamiView protein in Pfam
PF01569 PAP2, 1 hit
SMARTiView protein in SMART
SM00014 acidPPc, 1 hit
SUPFAMiSSF48317 SSF48317, 1 hit

Sequencei

Sequence statusi: Complete.

O14495-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQNYKYDKAI VPESKNGGSP ALNNNPRRSG SKRVLLICLD LFCLFMAGLP
60 70 80 90 100
FLIIETSTIK PYHRGFYCND ESIKYPLKTG ETINDAVLCA VGIVIAILAI
110 120 130 140 150
ITGEFYRIYY LKKSRSTIQN PYVAALYKQV GCFLFGCAIS QSFTDIAKVS
160 170 180 190 200
IGRLRPHFLS VCNPDFSQIN CSEGYIQNYR CRGDDSKVQE ARKSFFSGHA
210 220 230 240 250
SFSMYTMLYL VLYLQARFTW RGARLLRPLL QFTLIMMAFY TGLSRVSDHK
260 270 280 290 300
HHPSDVLAGF AQGALVACCI VFFVSDLFKT KTTLSLPAPA IRKEILSPVD
310
IIDRNNHHNM M
Length:311
Mass (Da):35,116
Last modified:January 1, 1998 - v1
Checksum:iCB3F60189044DA31
GO

Sequence cautioni

The sequence AAB50222 differs from that shown. Reason: Frameshift at position 225.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32K → M in AAV38396 (Ref. 7) Curated1
Sequence conflicti282T → M in AAH09196 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000889 mRNA Translation: BAA22594.1
AF017786 mRNA Translation: AAC63433.1
AF480883 mRNA Translation: AAO84481.1
AF043329 mRNA Translation: AAD02271.1
U79294 mRNA Translation: AAB50222.1 Frameshift.
AK312439 mRNA Translation: BAG35348.1
BT019589 mRNA Translation: AAV38396.1
CH471059 Genomic DNA Translation: EAX06651.1
CH471059 Genomic DNA Translation: EAX06652.1
CH471059 Genomic DNA Translation: EAX06653.1
BC009196 mRNA Translation: AAH09196.1
CCDSiCCDS604.1
RefSeqiNP_003704.3, NM_003713.4
UniGeneiHs.405156
Hs.619002

Genome annotation databases

EnsembliENST00000371250; ENSP00000360296; ENSG00000162407
GeneIDi8613
KEGGihsa:8613
UCSCiuc001cyj.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000889 mRNA Translation: BAA22594.1
AF017786 mRNA Translation: AAC63433.1
AF480883 mRNA Translation: AAO84481.1
AF043329 mRNA Translation: AAD02271.1
U79294 mRNA Translation: AAB50222.1 Frameshift.
AK312439 mRNA Translation: BAG35348.1
BT019589 mRNA Translation: AAV38396.1
CH471059 Genomic DNA Translation: EAX06651.1
CH471059 Genomic DNA Translation: EAX06652.1
CH471059 Genomic DNA Translation: EAX06653.1
BC009196 mRNA Translation: AAH09196.1
CCDSiCCDS604.1
RefSeqiNP_003704.3, NM_003713.4
UniGeneiHs.405156
Hs.619002

3D structure databases

ProteinModelPortaliO14495
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114171, 9 interactors
CORUMiO14495
IntActiO14495, 4 interactors
MINTiO14495
STRINGi9606.ENSP00000360296

Chemistry databases

SwissLipidsiSLP:000001642

PTM databases

DEPODiO14495
iPTMnetiO14495
PhosphoSitePlusiO14495

Polymorphism and mutation databases

BioMutaiPPAP2B

Proteomic databases

EPDiO14495
MaxQBiO14495
PaxDbiO14495
PeptideAtlasiO14495
PRIDEiO14495
ProteomicsDBi48039

Protocols and materials databases

DNASUi8613
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371250; ENSP00000360296; ENSG00000162407
GeneIDi8613
KEGGihsa:8613
UCSCiuc001cyj.3 human

Organism-specific databases

CTDi8613
DisGeNETi8613
EuPathDBiHostDB:ENSG00000162407.8
GeneCardsiPLPP3
HGNCiHGNC:9229 PLPP3
HPAiHPA028892
HPA072751
MIMi607125 gene
neXtProtiNX_O14495
OpenTargetsiENSG00000162407
PharmGKBiPA33553
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3030 Eukaryota
COG0671 LUCA
GeneTreeiENSGT00620000087654
HOGENOMiHOG000041307
HOVERGENiHBG002048
InParanoidiO14495
KOiK01080
OMAiQNYKCRG
OrthoDBiEOG091G0K5H
PhylomeDBiO14495
TreeFamiTF316040

Enzyme and pathway databases

ReactomeiR-HSA-1660661 Sphingolipid de novo biosynthesis
SignaLinkiO14495

Miscellaneous databases

ChiTaRSiPLPP3 human
GeneWikiiPPAP2B
GenomeRNAii8613
PROiPR:O14495
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162407 Expressed in 239 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_PPAP2B
GenevisibleiO14495 HS

Family and domain databases

InterProiView protein in InterPro
IPR028675 LPP3
IPR036938 P_Acid_Pase_2/haloperoxi_sf
IPR000326 P_Acid_Pase_2/haloperoxidase
PANTHERiPTHR10165:SF79 PTHR10165:SF79, 1 hit
PfamiView protein in Pfam
PF01569 PAP2, 1 hit
SMARTiView protein in SMART
SM00014 acidPPc, 1 hit
SUPFAMiSSF48317 SSF48317, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLPP3_HUMAN
AccessioniPrimary (citable) accession number: O14495
Secondary accession number(s): B2R651
, D3DQ52, Q5U0F7, Q96GW0, Q99782
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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