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Protein

Biotin--protein ligase

Gene

bpl1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase (By similarity).By similarity

Catalytic activityi

ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-196780 Biotin transport and metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase (EC:6.3.4.-)
Alternative name(s):
Biotin apo-protein ligase
Including the following 4 domains:
Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
Alternative name(s):
Holocarboxylase synthetase
Short name:
HCS
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:bpl1
ORF Names:SPBC30D10.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC30D10.07c
PomBaseiSPBC30D10.07c bpl1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003159701 – 631Biotin--protein ligaseAdd BLAST631

Proteomic databases

MaxQBiO14353
PaxDbiO14353
PRIDEiO14353

PTM databases

SwissPalmiO14353

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4896.SPBC30D10.07c.1

Structurei

3D structure databases

ProteinModelPortaliO14353
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini341 – 553BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST213

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated

Phylogenomic databases

HOGENOMiHOG000214585
InParanoidiO14353
KOiK01942
OMAiFFPGTCR
OrthoDBiEOG092C16UD
PhylomeDBiO14353

Family and domain databases

CDDicd16442 BPL, 1 hit
Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR019197 Biotin-prot_ligase_N
IPR004408 Biotin_CoA_COase_ligase
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
IPR029062 Class_I_gatase-like
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
PF09825 BPL_N, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00121 birA_ligase, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

O14353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLIYNGNG ASKICLLRTF QSLLPFVVPL YAMRFVDAST LEKEPWPAST
60 70 80 90 100
ALLVMPGGRD MGYCSSFNET IYRKITDFVK RGGAYLGLCA GGYFGSAVVD
110 120 130 140 150
FRMPDSDLNV VGKRKLQFFP GTCAGPTFPG FTYDSEDGAR RASIIVDGMQ
160 170 180 190 200
SSPVHTHIYF NGGGSFLETE NYSNVKVVAR YQETDFEKSA AIIYVKVGKG
210 220 230 240 250
NVVLTGIHPE FSAEGSPILD KRDEKTRLEL LSYILKLLGL KVPKDTSKCG
260 270 280 290 300
QPTLTDQYLF PNNVETKRFI EKALTNKVKN QDEDTLYTFQ FSDISSEIPE
310 320 330 340 350
HQLANLDISA DLSDSDNEIV KIWYGDEEKI CKKAKPSFDL ELYAKLINGC
360 370 380 390 400
RFGLPIIVAP VIRSTQTLLD KNYRFLDSTN TGFTVLGNYQ TAGRGRGQNM
410 420 430 440 450
WVSPYGTLAF SFIINVDAKN FSTTPIALFQ YLMALAVVRG IREYAPGYEN
460 470 480 490 500
IPAFIKWPND VYVRVDKGGI NFQGKQYMKL SGIIVTSNYR KNVLHLVVGC
510 520 530 540 550
GINVSNLGPT VSLNTLVDEW NKNSDNPRLE KFSFEKLLAS VLNQFDRYHR
560 570 580 590 600
LLLEEGFSLI LPEYYQYWLH SNQTVNLASG GKAIIQGITS DFGFLLAQLL
610 620 630
NENNEPTTKV VHLQPDGNSF DLMRNLITRK T
Length:631
Mass (Da):70,632
Last modified:January 1, 1998 - v1
Checksum:i37E08571359F5112
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAB10802.1
PIRiT40189
RefSeqiNP_596278.1, NM_001022199.2

Genome annotation databases

EnsemblFungiiSPBC30D10.07c.1; SPBC30D10.07c.1:pep; SPBC30D10.07c
GeneIDi2540476
KEGGispo:SPBC30D10.07c

Similar proteinsi

Entry informationi

Entry nameiBPL1_SCHPO
AccessioniPrimary (citable) accession number: O14353
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 1, 1998
Last modified: June 20, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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