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UniProtKB - O14215 (PRI1_SCHPO)

Entry version 128 (16 Jan 2019)
Sequence version 1 (01 Jan 1998)
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Protein

DNA primase small subunit

Gene

pri1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Miscellaneous

The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei66Sequence analysis1
Active sitei131Sequence analysis1
Active sitei133Sequence analysis1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • DNA primase activity Source: PomBase
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SPO-174411 Polymerase switching on the C-strand of the telomere
R-SPO-174430 Telomere C-strand synthesis initiation
R-SPO-68952 DNA replication initiation
R-SPO-68962 Activation of the pre-replicative complex
R-SPO-69091 Polymerase switching
R-SPO-69166 Removal of the Flap Intermediate
R-SPO-69183 Processive synthesis on the lagging strand

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA primase small subunit (EC:2.7.7.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pri1
Synonyms:spp1
ORF Names:SPAC6B12.10c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri284812 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002485 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome I

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		FungiDB:SPAC6B12.10c

Schizosaccharomyces pombe database

More...PomBasei		SPAC6B12.10c

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000467351 – 454DNA primase small subunitAdd BLAST454

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		O14215

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O14215

PRoteomics IDEntifications database

More...PRIDEi		O14215

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a small subunit and a large subunit.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
pri2O747612EBI-849063,EBI-849075

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		279746, 6 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-2092 DNA polymerase alpha:primase complex

Protein interaction database and analysis system

More...IntActi		O14215, 4 interactors

STRING: functional protein association networks

More...STRINGi		4896.SPAC6B12.10c.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...ProteinModelPortali		O14215

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O14215

Database of comparative protein structure models

More...ModBasei		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi143 – 153Zinc knuckle motifAdd BLAST11

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the eukaryotic-type primase small subunit family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000196041

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O14215

KEGG Orthology (KO)

More...KOi		K02684

Identification of Orthologs from Complete Genome Data

More...OMAi		VYYRRLF

Database for complete collections of gene phylogenies

More...PhylomeDBi		O14215

Family and domain databases

Conserved Domains Database

More...CDDi		cd04860 AE_Prim_S, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR002755 DNA_primase_S
IPR014052 DNA_primase_ssu_euk/arc

The PANTHER Classification System

More...PANTHERi		PTHR10536 PTHR10536, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01896 DNA_primase_S, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00335 primase_sml, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O14215-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVQIDELDD KDLDEIIANG TLDGAKQGAV DSETMIQYYR HLFPWKYLFQ 
        60         70         80         90        100
WLNHGPVVTN DFAHREFAFT LPNDAYIRYL SFSNWEELKK EALNLCPSRF 
       110        120        130        140        150
EVGPVYSANP RDRKTIRKST FHPLKKELVF DIDMTDYDDV RTCCSKTNIC 
       160        170        180        190        200
EKCWPFITIA VQVLDICFHE DFGFKHILWV YSGRRGIHAW ICDEIACSLD 
       210        220        230        240        250
DRSRRMIASY LQVVVGNPQG GVRLINNLKR PLHPHLTRSL NILKSAFVKI 
       260        270        280        290        300
VLEDQDPWAS KEGAENLLKL LPDKDLASAL RKKWEVDPER SSKNKWSDID 
       310        320        330        340        350
TVLASGSIAS ISPSVIAIAK QDIVLTYLYP RLDVEVSRHL NHLLKSPFCV 
       360        370        380        390        400
HPGTSRVCVP IDIERMDSFN PLKVPTVNDL LQELDKNSQN DNGHGPTMET 
       410        420        430        440        450
NTTENQKDNA RGQSNKGHGF STSLNPYTLY FKSFSSQLFK ETVGNKRKHE 

NLEF
Length:454
Mass (Da):52,009
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i71526FFAF7C2BBFA
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11078.1

Protein sequence database of the Protein Information Resource

More...PIRi		T39017

NCBI Reference Sequences

More...RefSeqi		NP_593765.1, NM_001019195.2

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		SPAC6B12.10c.1; SPAC6B12.10c.1:pep; SPAC6B12.10c

Database of genes from NCBI RefSeq genomes

More...GeneIDi		2543323

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		spo:SPAC6B12.10c

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11078.1
PIRiT39017
RefSeqiNP_593765.1, NM_001019195.2

3D structure databases

ProteinModelPortaliO14215
SMRiO14215
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279746, 6 interactors
ComplexPortaliCPX-2092 DNA polymerase alpha:primase complex
IntActiO14215, 4 interactors
STRINGi4896.SPAC6B12.10c.1

Proteomic databases

MaxQBiO14215
PaxDbiO14215
PRIDEiO14215

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6B12.10c.1; SPAC6B12.10c.1:pep; SPAC6B12.10c
GeneIDi2543323
KEGGispo:SPAC6B12.10c

Organism-specific databases

EuPathDBiFungiDB:SPAC6B12.10c
PomBaseiSPAC6B12.10c

Phylogenomic databases

HOGENOMiHOG000196041
InParanoidiO14215
KOiK02684
OMAiVYYRRLF
PhylomeDBiO14215

Enzyme and pathway databases

ReactomeiR-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SPO-174411 Polymerase switching on the C-strand of the telomere
R-SPO-174430 Telomere C-strand synthesis initiation
R-SPO-68952 DNA replication initiation
R-SPO-68962 Activation of the pre-replicative complex
R-SPO-69091 Polymerase switching
R-SPO-69166 Removal of the Flap Intermediate
R-SPO-69183 Processive synthesis on the lagging strand

Miscellaneous databases

Protein Ontology

More...PROi		PR:O14215

Family and domain databases

CDDicd04860 AE_Prim_S, 1 hit
InterProiView protein in InterPro
IPR002755 DNA_primase_S
IPR014052 DNA_primase_ssu_euk/arc
PANTHERiPTHR10536 PTHR10536, 1 hit
PfamiView protein in Pfam
PF01896 DNA_primase_S, 1 hit
TIGRFAMsiTIGR00335 primase_sml, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRI1_SCHPO
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O14215
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: January 16, 2019
This is version 128 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
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