UniProtKB - O14194 (GGT2_SCHPO)
Glutathione hydrolase proenzyme 2
ggt2
Functioni
Catalyzes the transfer of the gamma-glutamyl moiety of glutathione (GSH) and other gamma-glutamyl compounds to amino acids and peptides. Major GSH-degrading enzyme, catalyzing the hydrolytic release of L-glutamate from GSH. Plays a role in the turnover of the vacuolar GSH, serving as an alternative nitrogen source during nitrogen starvation (By similarity).
By similarity1 PublicationCatalytic activityi
- an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide]1 PublicationEC:2.3.2.21 Publication
- EC:3.4.19.13
- EC:3.4.19.13
: glutathione metabolism Pathwayi
This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 147 | GlutamateBy similarity | 1 | |
Active sitei | 420 | NucleophileBy similarity | 1 | |
Binding sitei | 438 | GlutamateBy similarity | 1 | |
Binding sitei | 440 | GlutamateBy similarity | 1 | |
Binding sitei | 459 | GlutamateBy similarity | 1 | |
Binding sitei | 462 | GlutamateBy similarity | 1 |
GO - Molecular functioni
- glutathione hydrolase activity Source: PomBase
- hypoglycin A gamma-glutamyl transpeptidase activity Source: UniProtKB-EC
- leukotriene C4 gamma-glutamyl transferase activity Source: UniProtKB-EC
- peptidyltransferase activity Source: GO_Central
GO - Biological processi
- cellular detoxification Source: PomBase
- glutathione catabolic process Source: PomBase
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Protease, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.2.2, 5613 |
Reactomei | R-SPO-174403, Glutathione synthesis and recycling R-SPO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-SPO-5423646, Aflatoxin activation and detoxification |
UniPathwayi | UPA00204 |
Protein family/group databases
MEROPSi | T03.011 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ggt2 ORF Names:SPAC56E4.06c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC56E4.06c, ggt2 |
VEuPathDBi | FungiDB:SPAC56E4.06c |
Subcellular locationi
Vacuole
- Vacuole membrane Curated; Single-pass type II membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Vacuole
- fungal-type vacuole Source: PomBase
- vacuolar membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 42 | CytoplasmicSequence analysisAdd BLAST | 42 | |
Transmembranei | 43 – 63 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 64 – 611 | LumenalSequence analysisAdd BLAST | 548 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247900 | 1 – 419 | Glutathione hydrolase 2 heavy chainBy similarityAdd BLAST | 419 | |
ChainiPRO_0000247901 | 420 – 611 | Glutathione hydrolase 2 light chainBy similarityAdd BLAST | 192 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 138 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 297 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 396 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | O14194 |
PaxDbi | O14194 |
PTM databases
iPTMneti | O14194 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 279542, 8 interactors |
STRINGi | 4896.SPAC56E4.06c.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 490 – 491 | Glutamate bindingBy similarity | 2 | |
Regioni | 512 – 513 | Glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2410, Eukaryota |
HOGENOMi | CLU_014813_4_0_1 |
InParanoidi | O14194 |
OMAi | MAHQRHG |
PhylomeDBi | O14194 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSPTDTTPLL YSWDDQSRHQ DPDWHKLRNY HGAWYRRISR RRFSQFIFAF
60 70 80 90 100
GLMTLFVLVY SISSNLHTPT QFTGHKVRGR RGAVASEVPV CSDIGVSMLA
110 120 130 140 150
DGGNAVDAAI ASTFCIGVVN FFSSGIGGGG FMLIKHPNET AQSLTFREIA
160 170 180 190 200
PGNVSKHMFD KNPMLAQVGP LSIAIPGELA GLYEAWKSHG LLDWSKLLEP
210 220 230 240 250
NVKLAREGFP VTRAMERVLK LPEMAHLLKD PIWQPILMPN GKVLRAGDKM
260 270 280 290 300
FRPAYAKTLE IIANKGIEPF YRGELTNSMV KFIQDNGGIV TVEDFGNYST
310 320 330 340 350
VFADALHTSY RGHDVYTCTL PTSGPALIEG LNILDGYPLN TPSLAFPKRL
360 370 380 390 400
HLEVEAMKWL SAGRTQFGDP DFLPLDHLDV VSKLLSKEFA SQIRNNISLS
410 420 430 440 450
KTYPWEHYNP SYDLPISHGT THVSTVDSNN LAVSITSTVN LLFGSQLMDP
460 470 480 490 500
VTGVVFNDQM DDFSIPGASN AFNLSPSPWN FIEPFKRPQS SSAPTILTDI
510 520 530 540 550
NGDFEMALGA SGGSRIVTAV LDSIIKRIDM DYDIESMVAS ARPHHQLLPD
560 570 580 590 600
ILILESGFSK SVATRMKKYG HKVWRLKQHD TPLSQIQAVT RHHSEYYGMS
610
DPRKYGQAAA Y
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY359853 Genomic DNA Translation: AAQ57121.1 CU329670 Genomic DNA Translation: CAB16397.1 |
PIRi | T38908 |
RefSeqi | NP_593273.1, NM_001018670.2 |
Genome annotation databases
EnsemblFungii | SPAC56E4.06c.1; SPAC56E4.06c.1:pep; SPAC56E4.06c |
GeneIDi | 2543110 |
KEGGi | spo:SPAC56E4.06c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY359853 Genomic DNA Translation: AAQ57121.1 CU329670 Genomic DNA Translation: CAB16397.1 |
PIRi | T38908 |
RefSeqi | NP_593273.1, NM_001018670.2 |
3D structure databases
SMRi | O14194 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 279542, 8 interactors |
STRINGi | 4896.SPAC56E4.06c.1 |
Protein family/group databases
MEROPSi | T03.011 |
PTM databases
iPTMneti | O14194 |
Proteomic databases
MaxQBi | O14194 |
PaxDbi | O14194 |
Genome annotation databases
EnsemblFungii | SPAC56E4.06c.1; SPAC56E4.06c.1:pep; SPAC56E4.06c |
GeneIDi | 2543110 |
KEGGi | spo:SPAC56E4.06c |
Organism-specific databases
PomBasei | SPAC56E4.06c, ggt2 |
VEuPathDBi | FungiDB:SPAC56E4.06c |
Phylogenomic databases
eggNOGi | KOG2410, Eukaryota |
HOGENOMi | CLU_014813_4_0_1 |
InParanoidi | O14194 |
OMAi | MAHQRHG |
PhylomeDBi | O14194 |
Enzyme and pathway databases
UniPathwayi | UPA00204 |
BRENDAi | 2.3.2.2, 5613 |
Reactomei | R-SPO-174403, Glutathione synthesis and recycling R-SPO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-SPO-5423646, Aflatoxin activation and detoxification |
Miscellaneous databases
PROi | PR:O14194 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGT2_SCHPO | |
Accessioni | O14194Primary (citable) accession number: O14194 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families