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Protein

Serine--tRNA ligase, cytoplasmic

Gene

SPAC29A4.15

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity).By similarity

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, mitochondrial (dia4), Serine--tRNA ligase, cytoplasmic (SPAC29A4.15)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei294SerineBy similarity1
Binding sitei396SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3
Nucleotide bindingi358 – 361ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
ORF Names:SPAC29A4.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC29A4.15
PomBaseiSPAC29A4.15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001221981 – 450Serine--tRNA ligase, cytoplasmicAdd BLAST450

Proteomic databases

MaxQBiO14018
PaxDbiO14018
PRIDEiO14018

PTM databases

iPTMnetiO14018

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer (By similarity).By similarity

Protein-protein interaction databases

BioGridi278639, 4 interactors
STRINGi4896.SPAC29A4.15.1

Structurei

3D structure databases

ProteinModelPortaliO14018
SMRiO14018
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 240Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000035937
InParanoidiO14018
KOiK01875
OMAiSPCFRRE
OrthoDBiEOG092C513S
PhylomeDBiO14018

Family and domain databases

CDDicd00770 SerRS_core, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR002317 Ser-tRNA-ligase_type_1
IPR015866 Ser-tRNA-synth_1_N
IPR033729 SerRS_core
IPR010978 tRNA-bd_arm
PANTHERiPTHR11778 PTHR11778, 1 hit
PfamiView protein in Pfam
PF02403 Seryl_tRNA_N, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PIRSFiPIRSF001529 Ser-tRNA-synth_IIa, 1 hit
PRINTSiPR00981 TRNASYNTHSER
SUPFAMiSSF46589 SSF46589, 1 hit
TIGRFAMsiTIGR00414 serS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O14018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINLFQVE KGGNPEIIRE SQRKRGADVG VVDKVIEMYK EWVSLRFELD
60 70 80 90 100
NTNKSINRVQ KEIGLKMKAK EDASELLEEK NSLTERKKNL IEQETAKNKE
110 120 130 140 150
MLNVVSSIGN IVHDSVPVSM DEDNNEIIRK WAPEGVTVEK KNCLSHHEVL
160 170 180 190 200
TRLDGYDPER GVKVSGHRGY FLRQYGVFFN LALIQYGLDF LEKRGYIALQ
210 220 230 240 250
APTMLNKDVM AKTAQLEQFD EELYKVIDGD EERYLIATSE QPISAYHSGE
260 270 280 290 300
WFEKPSEQLP LKYAGYSTCY RREAGSHGRD AWGIFRVHAF EKIEQFVLTD
310 320 330 340 350
PEKSWEAFTE MINHAEDFYK SLELPYRIVA IVSGALNNAA AKKYDLEAWF
360 370 380 390 400
PFQGEYKELV SCSNCTDYQS RNLEIRCGVK KMGDREKKYV HCLNSTLCAT
410 420 430 440 450
ERALCCILEN YQTPDGVNVP KVLQPYMGGK TFLPFTKELP KNSTSKKGKN
Length:450
Mass (Da):51,643
Last modified:January 1, 1998 - v1
Checksum:i052DE1D4DF7EA646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB10149.1
PIRiT38474
RefSeqiNP_594867.1, NM_001020296.2

Genome annotation databases

EnsemblFungiiSPAC29A4.15.1; SPAC29A4.15.1:pep; SPAC29A4.15
GeneIDi2542163
KEGGispo:SPAC29A4.15

Similar proteinsi

Entry informationi

Entry nameiSYSC_SCHPO
AccessioniPrimary (citable) accession number: O14018
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 20, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

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