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Protein

Dihydroxyacetone kinase 1

Gene

dak1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycerol dehydrogenase 1 (gld1)
  2. Dihydroxyacetone kinase 2 (dak2), Dihydroxyacetone kinase 1 (dak1)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateBy similarity1
Binding sitei109SubstrateBy similarity1
Active sitei221Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi413 – 416ATPBy similarity4
Nucleotide bindingi458 – 459ATPBy similarity2
Nucleotide bindingi512 – 513ATPBy similarity2
Nucleotide bindingi565 – 567ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerone kinase activity Source: PomBase
  • triokinase activity Source: UniProtKB-EC

GO - Biological processi

  • glycerol catabolic process Source: PomBase
  • glycerol metabolic process Source: PomBase

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycerol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.29 5613
ReactomeiR-SPO-70350 Fructose catabolism
UniPathwayi
UPA00617;UER00669

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 1 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 1
Alternative name(s):
Glycerone kinase 1
Triokinase 1
Triose kinase 1
Gene namesi
Name:dak1
ORF Names:SPAC22A12.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22A12.11
PomBaseiSPAC22A12.11 dak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215201 – 580Dihydroxyacetone kinase 1Add BLAST580

Proteomic databases

MaxQBiO13902
PaxDbiO13902
PRIDEiO13902

PTM databases

iPTMnetiO13902

Interactioni

Protein-protein interaction databases

BioGridi278300, 13 interactors
STRINGi4896.SPAC22A12.11.1

Structurei

3D structure databases

ProteinModelPortaliO13902
SMRiO13902
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 349DhaKPROSITE-ProRule annotationAdd BLAST342
Domaini384 – 580DhaLPROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 56Substrate bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi161 – 166Poly-Ala6

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000234158
InParanoidiO13902
KOiK00863
OMAiEPAHVGF
OrthoDBiEOG092C1OGC
PhylomeDBiO13902

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit

Sequencei

Sequence statusi: Complete.

O13902-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDKHFINDPE VLVLDGLKSL ADMNKTLTVH EEGKFIYFHD YNKKNVSVIS
60 70 80 90 100
GGGAGHEPTH SSFVGKGMLT AAVSGSIFAS PSSKQIYTGI KQVESEAGTL
110 120 130 140 150
VICKNYTGDI LHFGMALEKQ RTAGKKAELI AVADDVSVGR KKSGKVGRRG
160 170 180 190 200
LSGTVLVHKI AGAAAARGLP LEAVTTIAKA AIDNLVSIGA SLAHVHVPGH
210 220 230 240 250
EPIAKEDEMK HDEMELGMGI HNEPGCKRIS PIPSIDDLIA QMLKQMLDQS
260 270 280 290 300
DKDRAYVKIE GDDEVVLLMN NLGGLSMLEF SAISHKVKEA LAKEYKINPV
310 320 330 340 350
RIFAGPFTTS LNGLGFGITL LRTTDRVKVE GEEYSLVDLI DQPVEAIGWP
360 370 380 390 400
LCQPSDLKSK NKIGNVSIEE GQKDVKSPVT VDKEKVRQAI VNSMENLIKA
410 420 430 440 450
EPKITKFDTM AGDGDCGTTL KRGAEGVLKF VKSDKFSDDP IRIVRDIADV
460 470 480 490 500
IEDNMDGTSG ALYAIFFHGF AKGMKDTLEK SKDISSKTWA AGLKVALDTL
510 520 530 540 550
FKYTPARPGD STMCDALVPF VETFVKTNDL NAAVEEARKG ADATADMQAK
560 570 580
LGRAVYVGDD VKVPDAGALG VVAIVEGFTK
Length:580
Mass (Da):62,316
Last modified:January 1, 1998 - v1
Checksum:i8AC984318610E08D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010078 Genomic DNA Translation: BAA24186.1
CU329670 Genomic DNA Translation: CAB16581.1
PIRiT43310
RefSeqiNP_593241.1, NM_001018638.2

Genome annotation databases

EnsemblFungiiSPAC22A12.11.1; SPAC22A12.11.1:pep; SPAC22A12.11
GeneIDi2541809
KEGGispo:SPAC22A12.11

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010078 Genomic DNA Translation: BAA24186.1
CU329670 Genomic DNA Translation: CAB16581.1
PIRiT43310
RefSeqiNP_593241.1, NM_001018638.2

3D structure databases

ProteinModelPortaliO13902
SMRiO13902
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278300, 13 interactors
STRINGi4896.SPAC22A12.11.1

PTM databases

iPTMnetiO13902

Proteomic databases

MaxQBiO13902
PaxDbiO13902
PRIDEiO13902

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22A12.11.1; SPAC22A12.11.1:pep; SPAC22A12.11
GeneIDi2541809
KEGGispo:SPAC22A12.11

Organism-specific databases

EuPathDBiFungiDB:SPAC22A12.11
PomBaseiSPAC22A12.11 dak1

Phylogenomic databases

HOGENOMiHOG000234158
InParanoidiO13902
KOiK00863
OMAiEPAHVGF
OrthoDBiEOG092C1OGC
PhylomeDBiO13902

Enzyme and pathway databases

UniPathwayi
UPA00617;UER00669

BRENDAi2.7.1.29 5613
ReactomeiR-SPO-70350 Fructose catabolism

Miscellaneous databases

PROiPR:O13902

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDAK1_SCHPO
AccessioniPrimary (citable) accession number: O13902
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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