UniProtKB - O13826 (RFP1_SCHPO)
Protein
E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1
Gene
rfp1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Involved in maintaining genome stability where it acts in the cellular response to DNA damage. Has a role in meiosis.4 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 189 – 238 | RING-typePROSITE-ProRule annotationAdd BLAST | 50 |
GO - Molecular functioni
- DNA binding Source: PomBase
- SUMO-ubiquitin ligase activity Source: PomBase
- zinc ion binding Source: PomBase
GO - Biological processi
- DNA repair Source: PomBase
- meiotic cell cycle Source: UniProtKB-KW
- negative regulation of protein sumoylation Source: PomBase
- protein ubiquitination Source: UniProtKB-UniPathway
- ubiquitin-dependent protein catabolic process Source: PomBase
Keywordsi
Molecular function | Transferase |
Biological process | Meiosis, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1 (EC:2.3.2.27)Alternative name(s): Meiotically up-regulated gene 140 protein RING finger protein 1 RING-type E3 ubiquitin transferase rfp1Curated |
Gene namesi | Name:rfp1 Synonyms:mug140 ORF Names:SPAC19A8.10 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC19A8.10, rfp1 |
VEuPathDBi | FungiDB:SPAC19A8.10 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Nucleus
- nuclear chromatin Source: PomBase
- nucleus Source: PomBase
- SUMO-targeted ubiquitin ligase complex Source: PomBase
Other locations
- site of double-strand break Source: PomBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056333 | 1 – 254 | E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1Add BLAST | 254 |
Proteomic databases
PaxDbi | O13826 |
Interactioni
Subunit structurei
Part of an E3 ubiquitin complex including rfp1, rfp2 and slx8.
Interacts with slx8, chk1, nse5, pmt3 and rad60.
3 PublicationsBinary interactionsi
Hide detailsO13826
With | #Exp. | IntAct |
---|---|---|
chk1 [P34208] | 3 | EBI-3647269,EBI-768535 |
pmt3 [O13351] | 5 | EBI-3647269,EBI-966336 |
slx8 [P87176] | 5 | EBI-3647269,EBI-7588105 |
Protein-protein interaction databases
BioGRIDi | 278999, 18 interactors |
IntActi | O13826, 10 interactors |
MINTi | O13826 |
STRINGi | 4896.SPAC19A8.10.1 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 54 – 57 | Poly-Arg | 4 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 189 – 238 | RING-typePROSITE-ProRule annotationAdd BLAST | 50 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502SDJU, Eukaryota |
HOGENOMi | CLU_043321_1_0_1 |
InParanoidi | O13826 |
OMAi | PRCHEEL |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038886, E3_SLX5/Rfp1 IPR009078, Ferritin-like_SF IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR28042, PTHR28042, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O13826-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQFNGSNGID ESSVIDLTRS PSPPVETSIS STNIIDLDAI PDDSFPSSPV
60 70 80 90 100
LSPRRRRMNR RRNERSRNFP SNHLSYLEDM IYLGPQVSTR RSSSRRDLMG
110 120 130 140 150
MIARTFPEFS SVNSLSPSLF QLIVNRMRFD AIHPEWTNGS DDEYFSNHFE
160 170 180 190 200
ESYDDFTSSL ENIKQSYKPP GPPKSGFTRS FNNDTLMVCP RCQEPLGTSK
210 220 230 240 250
SKEKSALWAT KCGHVYCGSC AKVLKTSKRS QSKCLVNDCG RYLNTKNAMW
ELFY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB11646.1 |
PIRi | T37951 |
RefSeqi | NP_593782.1, NM_001019211.2 |
Genome annotation databases
EnsemblFungii | SPAC19A8.10.1; SPAC19A8.10.1:pep; SPAC19A8.10 |
GeneIDi | 2542542 |
KEGGi | spo:SPAC19A8.10 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB11646.1 |
PIRi | T37951 |
RefSeqi | NP_593782.1, NM_001019211.2 |
3D structure databases
SMRi | O13826 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 278999, 18 interactors |
IntActi | O13826, 10 interactors |
MINTi | O13826 |
STRINGi | 4896.SPAC19A8.10.1 |
Proteomic databases
PaxDbi | O13826 |
Genome annotation databases
EnsemblFungii | SPAC19A8.10.1; SPAC19A8.10.1:pep; SPAC19A8.10 |
GeneIDi | 2542542 |
KEGGi | spo:SPAC19A8.10 |
Organism-specific databases
PomBasei | SPAC19A8.10, rfp1 |
VEuPathDBi | FungiDB:SPAC19A8.10 |
Phylogenomic databases
eggNOGi | ENOG502SDJU, Eukaryota |
HOGENOMi | CLU_043321_1_0_1 |
InParanoidi | O13826 |
OMAi | PRCHEEL |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:O13826 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038886, E3_SLX5/Rfp1 IPR009078, Ferritin-like_SF IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR28042, PTHR28042, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFP1_SCHPO | |
Accessioni | O13826Primary (citable) accession number: O13826 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names