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UniProtKB - O13395 (CHS6_USTMA)
Protein
Chitin synthase 6
Gene
CHS6
Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Functioni
Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.
1 PublicationCatalytic activityi
- [(1→4)-N-acetyl-β-D-glucosaminyl](n) + UDP-N-acetyl-α-D-glucosamine = [(1→4)-N-acetyl-β-D-glucosaminyl](n+1) + H+ + UDPEC:2.4.1.16
GO - Molecular functioni
- chitin synthase activity Source: GO_Central
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitin synthase 6 (EC:2.4.1.16)Alternative name(s): Chitin-UDP acetyl-glucosaminyl transferase 6 Class-V chitin synthase 6 |
Gene namesi | Name:CHS6 ORF Names:UMAG_10367 |
Organismi | Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) |
Taxonomic identifieri | 237631 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_10367 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex.2 Publications
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell periphery Source: GO_Central
- cell septum Source: GO_Central
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 108 – 128 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 374 – 394 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 762 – 782 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 795 – 815 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 822 – 842 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193724 | 1 – 1180 | Chitin synthase 6Add BLAST | 1180 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 737 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | O13395 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1119 – 1174 | DEK C-terminalSequence analysisAdd BLAST | 56 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | O13395 |
OrthoDBi | 134286at2759 |
Family and domain databases
Gene3Di | 3.10.120.10, 2 hits |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR014876, DEK_C IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 2 hits PF08766, DEK_C, 1 hit |
SMARTi | View protein in SMART SM01117, Cyt-b5, 2 hits |
SUPFAMi | SSF53448, SSF53448, 1 hit SSF55856, SSF55856, 1 hit |
i Sequence
Sequence statusi: Complete.
O13395-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTKDAHDPF ATPLETITHD PLAETAGLKA DYDKRAYGEA NPALMGTLEK
60 70 80 90 100
DDMANRPIDA VEEVPTTSIR KWWVRLTWFT TWWIPSFVLS KCGGMKRPDV
110 120 130 140 150
QMAWREKFTI CAIIFWLCAI ILFYIIAFGR LLCPDYDKAW NLSQLSQHAG
160 170 180 190 200
ENDYYAAVRG TVYDFSKFYK GDHSDITNLQ TSSDLMLQLA GQDLTGYFPV
210 220 230 240 250
PLSVGCQSLV SDTSLALMPT ANNTPLISQA IHTSGPLQGD TSSKLHDINW
260 270 280 290 300
YPDRFLPFVK KLRKGYYVYS KKDLANQGQW RNWAVIHGKV YDLSNYLNTV
310 320 330 340 350
STYQTNPAYS FLDSSIVSLF KSQAGSDITA DFEDAMSTFN QTYRGATQAC
360 370 380 390 400
LDNVFYVGRT DFRDTARCEV QNYLLLAFSV LLVTTVLAKF IAALQLGTKR
410 420 430 440 450
SPEQQDKFVI CQVPCYTEGE EELRKTIDSL AGLEYDDKRK LLFLICDGMI
460 470 480 490 500
VGSGNDRSTP RIVLDILGVD PKIDPEPLMF KSVAEGSKQL NYAKVYSGLY
510 520 530 540 550
EFEGHVVPYI VVVKVGRPSE RSRPGNRGKR DSQILLMRYL NRVHFDAPMF
560 570 580 590 600
PLELEIYHQM KNVIGIDPAF YEYILMVDAD TRVEADGLNR LVANCADDSS
610 620 630 640 650
IIAICGETTL DNAEGSWWTM IQVYEYYISH HLSKAFESLF GSVTCLPGCF
660 670 680 690 700
SLYRIRSSDK GRPLFISNRI IDDYSENRVD TLHKKNLLHL GEDRYLTTLV
710 720 730 740 750
LKNFPSFRTK FVPDAKALTS APDRFGVLLS QRRRWINSTV HNLAELVLMP
760 770 780 790 800
ELCGFCLFSM RFIVFIDLLG TVILPATAVY LVYLIVTVAT KSAPIPYISI
810 820 830 840 850
AMIAAVYGLQ AILFLLKRQW QYIGWLVIYI LAYPVFSFFL PIYSFWHMDD
860 870 880 890 900
FSWGNTRIVV GEKGNKKIVA GTDDEPYDDT MIPLKRFSEY QREVWEEEAA
910 920 930 940 950
APSMRSGMTG ASGPFGNSQA ILHSGPPSVY RAGGSAYAGS VAGSDYGAGL
960 970 980 990 1000
GDYYQNTNVL QKPAHSRQTS AAALSQMGGS QAASMMFGTG TPSVYGMAGM
1010 1020 1030 1040 1050
GSMYGMPGSS ASMYGLPNPM MNTTASMYGL PPMLANPLGA NHSPAHSDIG
1060 1070 1080 1090 1100
VSMPVSQQNT GGSHIWAQPP EAATVAANSG RGSGMQARPV STLSALNATN
1110 1120 1130 1140 1150
PFGVTAVARA LAVNEASDPT DEEIKSAVQT YLANQPSLMN VTKRSVREAL
1160 1170 1180
VAAFPNAELS YKKSMINKAI DDTLSGGAQA
Sequence cautioni
The sequence AAB84285 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 196 | G → R in AAB84285 (PubMed:9454647).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030554 Genomic DNA Translation: AAB84285.1 Frameshift. CM003146 Genomic DNA Translation: KIS69073.1 |
PIRi | T42022 |
RefSeqi | XP_011389509.1, XM_011391207.1 |
Genome annotation databases
EnsemblFungii | KIS69073; KIS69073; UMAG_10367 |
GeneIDi | 23566410 |
KEGGi | uma:UMAG_10367 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030554 Genomic DNA Translation: AAB84285.1 Frameshift. CM003146 Genomic DNA Translation: KIS69073.1 |
PIRi | T42022 |
RefSeqi | XP_011389509.1, XM_011391207.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 5270.UM03052P0 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
Proteomic databases
PRIDEi | O13395 |
Genome annotation databases
EnsemblFungii | KIS69073; KIS69073; UMAG_10367 |
GeneIDi | 23566410 |
KEGGi | uma:UMAG_10367 |
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_10367 |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | O13395 |
OrthoDBi | 134286at2759 |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Miscellaneous databases
PHI-basei | PHI:1116 PHI:389 |
Family and domain databases
Gene3Di | 3.10.120.10, 2 hits |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR014876, DEK_C IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 2 hits PF08766, DEK_C, 1 hit |
SMARTi | View protein in SMART SM01117, Cyt-b5, 2 hits |
SUPFAMi | SSF53448, SSF53448, 1 hit SSF55856, SSF55856, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS6_USTMA | |
Accessioni | O13395Primary (citable) accession number: O13395 Secondary accession number(s): A0A0D1CR95, Q4PA11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 23, 2022 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families