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UniProtKB - O13394 (CHS5_USTMA)
Protein
Chitin synthase 5
Gene
CHS5
Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Functioni
Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.
2 PublicationsCatalytic activityi
- [(1→4)-N-acetyl-β-D-glucosaminyl](n) + UDP-N-acetyl-α-D-glucosamine = [(1→4)-N-acetyl-β-D-glucosaminyl](n+1) + H+ + UDPEC:2.4.1.16
GO - Molecular functioni
- chitin synthase activity Source: GO_Central
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitin synthase 5 (EC:2.4.1.16)Alternative name(s): Chitin-UDP acetyl-glucosaminyl transferase 5 Class-IV chitin synthase 5 |
Gene namesi | Name:CHS5 ORF Names:UMAG_10277 |
Organismi | Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) |
Taxonomic identifieri | 237631 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_10277 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Other locations
- Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place (By similarity). Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell periphery Source: GO_Central
- cell septum Source: GO_Central
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 326 | CytoplasmicCuratedAdd BLAST | 326 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 348 – 364 | ExtracellularCuratedAdd BLAST | 17 | |
Transmembranei | 365 – 385 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 386 – 624 | CytoplasmicCuratedAdd BLAST | 239 | |
Transmembranei | 625 – 645 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 646 – 1176 | ExtracellularCuratedAdd BLAST | 531 | |
Transmembranei | 1177 – 1197 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1198 – 1202 | CytoplasmicCurated | 5 | |
Transmembranei | 1203 – 1223 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1224 – 1227 | ExtracellularCurated | 4 | |
Transmembranei | 1228 – 1248 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1249 – 1609 | CytoplasmicCuratedAdd BLAST | 361 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193723 | 1 – 1609 | Chitin synthase 5Add BLAST | 1609 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 654 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 1015 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 1144 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
GlycoproteinStructurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 188 | DisorderedSequence analysisAdd BLAST | 188 | |
Regioni | 248 – 280 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 294 – 320 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 729 – 767 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 1354 – 1381 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 1399 – 1609 | DisorderedSequence analysisAdd BLAST | 211 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 20 – 55 | Pro residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 59 – 80 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 97 – 134 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 145 – 168 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 170 – 188 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 303 – 317 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 743 – 765 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 1354 – 1377 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 1435 – 1449 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1504 – 1518 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1526 – 1555 | Polar residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 1574 – 1593 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 1594 – 1609 | Pro residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | O13394 |
OMAi | FPLAHTM |
OrthoDBi | 134286at2759 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
O13394-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPFESLPDA ARPAQGTRPN RSDGPPPLPP LTIPGSTGRP QNPIFSPPPN
60 70 80 90 100
LHHILPPGYL PQQQQQQQQQ QQQQQQRSQQ PFFSPPPPDA MSPPLGSHQA
110 120 130 140 150
YLNSTSSQPT QRLPGISFQE PQRMPVQRSG PSRDSVKSYG DDKRSINDPN
160 170 180 190 200
SSSTALTQVN SLDPESGFSA HNDTFRRKKS LVRPDRERMD PSHRQWYYRN
210 220 230 240 250
HAAHMDVMAA SGGRVGYMPS TTGHLPQHGA APHGSGMAGI VGPGGGLSGL
260 270 280 290 300
GVTGPTNVPP GGLGRAPPLR RGKSLLGRDE DQVETGINVL KRGVSLRRKQ
310 320 330 340 350
SKSGNKPSKE VPRDLGESKT SRIAPGPVGG WMIYCYILTI CCPGPFLRIF
360 370 380 390 400
GIRTPEQQRA WREKMGLIGI ITLIMAAVGF LTFGFTQTVC GQQPDRYTLG
410 420 430 440 450
TIDVGSMTFN GYDYSFDGFI HPQVGPFGAD TIYNRTNPIY SEPWSSGGQD
460 470 480 490 500
GSLLFQKIGA ACTGIISNRA GGAQPERYFD CTLVRQDGKG GYANSTMPMC
510 520 530 540 550
HTGSIVDQFN DGAQPRNSVL KKRGQVSLQW NNVTDPARNL AVYRGSVLDL
560 570 580 590 600
NRLNNLTTGL SYPELYDTLK RRNDSWAGRD VTSAVMRQRL EREFQCLEQI
610 620 630 640 650
TRVGFIDSET IGCVASKVEL YLSLVFIIGV VAIKFFMAVM FGWFISWRLG
660 670 680 690 700
NYANETYEQR MKRAAEIEQW SDDIYRPAPA GYRPNARKHK SFLPAKSRFS
710 720 730 740 750
VADPLSLKSG SRAPMPLSEK RMTRASRLGV ASPLGGSPPG SPSVAGGRSS
760 770 780 790 800
ASLAPAHSRR SSFSGSPAEG AMGVCPFPLH NTIPQPGPDY RPFGFQLAHS
810 820 830 840 850
ICLVTAYSES FEGLRTTLDS LATTDYPNSH KLLLVIADGI VKGAGSDIST
860 870 880 890 900
PDICLSMMKD LVIPAEEVEG NSYVAIADGY KRHNMCKIYA GFYDYDDETV
910 920 930 940 950
ERSKQQRVPM ILVAKCGTPL EADSAKPGNR GKRDSQVLLM AFMQKVMFDE
960 970 980 990 1000
RMTAFEYEFF NSIWRVTGVS PDNYEIVLCV DADTKVFPDS LSRMVACMVE
1010 1020 1030 1040 1050
DPEIMGLCGE TKIANKSETW VTMIQVFEYY ISHHQTKAFE ACFGGVTCLP
1060 1070 1080 1090 1100
GCFSAYRIKA PKGPHGYWVP ILANPDIVEH YSENVVDTLH KKNLLLLGED
1110 1120 1130 1140 1150
RYLTTLMLKT FPKRKMMFVP QAVCKTIVPD TFRILLSQRR RWINSTVHNL
1160 1170 1180 1190 1200
FELVMVNDLC GTFCFSMRFV VFMELTGTLV LPAAIAFTLY VVVQAFLPNV
1210 1220 1230 1240 1250
PTPTIPLILL ALILGLPGIL IVVTSRKIAY VGWMLIYLLS LPIWNFVLPL
1260 1270 1280 1290 1300
YAYWHMDDFS WGATRVVQGE NKKDNHGDAD GKFDPSHIVM KRWAEFERER
1310 1320 1330 1340 1350
RWKSGTHSRD STYDVVQRTG SPERAGSTRY SVVSSDTFHS SPFGQHDQFG
1360 1370 1380 1390 1400
RALPNAMSSS SASQFGPDVS EVSHSKSPSG ARARLDAVPL LELPAPLATD
1410 1420 1430 1440 1450
AKHRSGASPT GTVVVPRPRA TSPAPLPHNS GHPALGSVSA FSPTQHSAGR
1460 1470 1480 1490 1500
LPTLPGAATY EAYPHTDAAD EERRPMIGST SSSPDPEPRR YIGPDAGVRH
1510 1520 1530 1540 1550
GNVSTEQRYP TVSESAYPMQ AYTAEPETDG SASPTPAQQG FNAANSNQQT
1560 1570 1580 1590 1600
RPLTRGFSLV DDGPVASAQG VRQVQRGARR SQMPNSAASP PPANRTGNLP
PGAAPPSFD
Sequence cautioni
The sequence AAB84284 differs from that shown. Reason: Frameshift.Curated
The sequence AAB84284 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | I → S in AAB84284 (PubMed:9454647).Curated | 1 | |
Sequence conflicti | 75 – 76 | Missing in AAB84284 (PubMed:9454647).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030553 Genomic DNA Translation: AAB84284.1 Sequence problems. CM003145 Genomic DNA Translation: KIS69221.1 |
PIRi | T42020 |
RefSeqi | XP_011389165.1, XM_011390863.1 |
Genome annotation databases
EnsemblFungii | KIS69221; KIS69221; UMAG_10277 |
GeneIDi | 23566330 |
KEGGi | uma:UMAG_10277 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF030553 Genomic DNA Translation: AAB84284.1 Sequence problems. CM003145 Genomic DNA Translation: KIS69221.1 |
PIRi | T42020 |
RefSeqi | XP_011389165.1, XM_011390863.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 5270.UM02569P0 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
Genome annotation databases
EnsemblFungii | KIS69221; KIS69221; UMAG_10277 |
GeneIDi | 23566330 |
KEGGi | uma:UMAG_10277 |
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_10277 |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | O13394 |
OMAi | FPLAHTM |
OrthoDBi | 134286at2759 |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Miscellaneous databases
PHI-basei | PHI:98 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS5_USTMA | |
Accessioni | O13394Primary (citable) accession number: O13394 Secondary accession number(s): A0A0D1DZZ4, Q4PBE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | September 16, 2015 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families