DNA replication fork-blocking protein FOB1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• metal ion binding Source: UniProtKB-KW
• rDNA spacer replication fork barrier binding Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.6

    "The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork."
    Kobayashi T.
    Mol. Cell. Biol. 23:9178-9188(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, MUTAGENESIS OF HIS-159; HIS-164; CYS-193; CYS-196 AND ASP-291.

GO - Biological processⁱ

• chromatin organization Source: SGD <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae."
    Zaman S., Choudhury M., Jiang J.C., Srivastava P., Mohanty B.K., Danielson C., Humphrey S.J., Jazwinski S.M., Bastia D.
    Mol. Cell. Biol. 36:1451-1463(2016) [PubMed] [Europe PMC] [Abstract]
• chromatin silencing at rDNA Source: SGDInferred from direct assayⁱ
  • "Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing."
    Huang J., Moazed D.
    Genes Dev. 17:2162-2176(2003) [PubMed] [Europe PMC] [Abstract]
• chromosome segregation Source: SGDInferred from genetic interactionⁱ
  • "Transcription of ribosomal genes can cause nondisjunction."
    Machin F., Torres-Rosell J., De Piccoli G., Carballo J.A., Cha R.S., Jarmuz A., Aragon L.
    J. Cell Biol. 173:893-903(2006) [PubMed] [Europe PMC] [Abstract]
• DNA recombination Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.1

    "A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities."
    Kobayashi T., Horiuchi T.
    Genes Cells 1:465-474(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
• dsDNA loop formation Source: SGDInferred from mutant phenotypeⁱ
  • "Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae."
    Zaman S., Choudhury M., Jiang J.C., Srivastava P., Mohanty B.K., Danielson C., Humphrey S.J., Jazwinski S.M., Bastia D.
    Mol. Cell. Biol. 36:1451-1463(2016) [PubMed] [Europe PMC] [Abstract]
• extrachromosomal circular DNA accumulation involved in cell aging Source: SGDInferred from mutant phenotypeⁱ
  • Ref.5

    "Elimination of replication block protein Fob1 extends the life span of yeast mother cells."
    Defossez P.A., Prusty R., Kaeberlein M., Lin S.J., Ferrigno P., Silver P.A., Keil R.L., Guarente L.
    Mol. Cell 3:447-455(1999) [PubMed] [Europe PMC] [Abstract]

    Cited for: SUBCELLULAR LOCATION.
• maintenance of rDNA Source: SGDInferred from genetic interactionⁱ
  • "Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast."
    Ide S., Saka K., Kobayashi T.
    PLoS Genet. 9:e1003410-e1003410(2013) [PubMed] [Europe PMC] [Abstract]
• negative regulation of DNA replication Source: SGDInferred from direct assayⁱ
  • Ref.6

    "The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork."
    Kobayashi T.
    Mol. Cell. Biol. 23:9178-9188(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, MUTAGENESIS OF HIS-159; HIS-164; CYS-193; CYS-196 AND ASP-291.
• positive regulation of DNA recombination Source: SGDInferred from mutant phenotypeⁱ
  • "Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity."
    Peng J., He M.H., Duan Y.M., Liu Y.T., Zhou J.Q.
    PLoS Genet. 11:e1005071-e1005071(2015) [PubMed] [Europe PMC] [Abstract]
• protein localization to nucleolar rDNA repeats Source: SGDInferred from mutant phenotypeⁱ
  • "The cis element and factors required for condensin recruitment to chromosomes."
    Johzuka K., Horiuchi T.
    Mol. Cell 34:26-35(2009) [PubMed] [Europe PMC] [Abstract]
• rDNA condensation Source: SGDInferred from mutant phenotypeⁱ
  • "Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit."
    Waples W.G., Chahwan C., Ciechonska M., Lavoie B.D.
    Mol. Biol. Cell 20:245-255(2009) [PubMed] [Europe PMC] [Abstract]
• regulation of cell aging Source: SGDInferred from mutant phenotypeⁱ
  • "Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity."
    Peng J., He M.H., Duan Y.M., Liu Y.T., Zhou J.Q.
    PLoS Genet. 11:e1005071-e1005071(2015) [PubMed] [Europe PMC] [Abstract]
• replication fork arrest at rDNA repeats Source: SGDInferred from mutant phenotypeⁱ
  • "Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae."
    Zaman S., Choudhury M., Jiang J.C., Srivastava P., Mohanty B.K., Danielson C., Humphrey S.J., Jazwinski S.M., Bastia D.
    Mol. Cell. Biol. 36:1451-1463(2016) [PubMed] [Europe PMC] [Abstract]
• replicative cell aging Source: SGDInferred from mutant phenotypeⁱ
  • "Genes determining yeast replicative life span in a long-lived genetic background."
    Kaeberlein M., Kirkland K.T., Fields S., Kennedy B.K.
    Mech. Ageing Dev. 126:491-504(2005) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Zinc finger

Keywords - Domainⁱ

Phylogenomic databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS 
        60         70         80         90        100
MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT 
       110        120        130        140        150
ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH 
       160        170        180        190        200
VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG 
       210        220        230        240        250
KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR 
       260        270        280        290        300
SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE 
       310        320        330        340        350
EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG 
       360        370        380        390        400
PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM 
       410        420        430        440        450
AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD 
       460        470        480        490        500
LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP 
       510        520        530        540        550
MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ 
       560 
QGNGDQTRDF GTSMEL                                      

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome IV
   Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names