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UniProtKB - O13329 (FOB1_YEAST)
Protein
DNA replication fork-blocking protein FOB1
Gene
FOB1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for replication fork blocking activity at the replication fork barrier (RFB) site in rDNA and for recombination hot-spot (HOT1) activity, regulating the recombination rate and the number of rDNA copies. Binds directly to two separated sequences in the RFB.
3 PublicationsMiscellaneous
Present with 1510 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 159 – 196 | C2H2-type1 PublicationAdd BLAST | 38 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- rDNA spacer replication fork barrier binding Source: SGD
GO - Biological processi
- chromatin organization Source: SGD
- chromosome segregation Source: SGD
- DNA recombination Source: SGD
- extrachromosomal circular DNA accumulation involved in cell aging Source: SGD
- maintenance of DNA repeat elements Source: SGD
- maintenance of rDNA Source: SGD
- negative regulation of DNA replication Source: SGD
- positive regulation of DNA recombination Source: SGD
- protein localization to nucleolar rDNA repeats Source: SGD
- rDNA condensation Source: SGD
- rDNA heterochromatin assembly Source: SGD
- regulation of cell aging Source: SGD
- replication fork arrest at rDNA repeats Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA recombination, DNA replication |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication fork-blocking protein FOB11 Publication |
Gene namesi | Ordered Locus Names:YDR110WImported ORF Names:YD9727.06 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002517, FOB1 |
VEuPathDBi | FungiDB:YDR110W |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Nucleus
- nucleolus Source: SGD
Other locations
- rDNA heterochromatin Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 159 | H → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication | 1 | |
Mutagenesisi | 164 | H → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication | 1 | |
Mutagenesisi | 193 | C → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication | 1 | |
Mutagenesisi | 196 | C → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication | 1 | |
Mutagenesisi | 291 | D → A: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087323 | 1 – 566 | DNA replication fork-blocking protein FOB1Add BLAST | 566 |
Proteomic databases
MaxQBi | O13329 |
PaxDbi | O13329 |
PRIDEi | O13329 |
PTM databases
iPTMneti | O13329 |
Interactioni
Subunit structurei
Interacts with NSI1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 32168, 112 interactors |
DIPi | DIP-870N |
IntActi | O13329, 35 interactors |
MINTi | O13329 |
STRINGi | 4932.YDR110W |
Miscellaneous databases
RNActi | O13329, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 466 – 566 | DisorderedSequence analysisAdd BLAST | 101 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 43 | Basic and acidic residuesSequence analysisAdd BLAST | 43 | |
Compositional biasi | 466 – 492 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 517 – 566 | Polar residuesSequence analysisAdd BLAST | 50 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 159 – 196 | C2H2-type1 PublicationAdd BLAST | 38 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502R9VR, Eukaryota |
HOGENOMi | CLU_030376_1_0_1 |
InParanoidi | O13329 |
OMAi | FSHFANI |
i Sequence
Sequence statusi: Complete.
O13329-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS
60 70 80 90 100
MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT
110 120 130 140 150
ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH
160 170 180 190 200
VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG
210 220 230 240 250
KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR
260 270 280 290 300
SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE
310 320 330 340 350
EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG
360 370 380 390 400
PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM
410 420 430 440 450
AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD
460 470 480 490 500
LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP
510 520 530 540 550
MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ
560
QGNGDQTRDF GTSMEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | T → A in AAB66897 (PubMed:9078378).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013245 Genomic DNA Translation: AAB66897.1 Z48758 Genomic DNA Translation: CAA88664.1 BK006938 Genomic DNA Translation: DAA11956.1 |
PIRi | S52676 |
RefSeqi | NP_010395.1, NM_001180418.1 |
Genome annotation databases
EnsemblFungii | YDR110W_mRNA; YDR110W; YDR110W |
GeneIDi | 851688 |
KEGGi | sce:YDR110W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013245 Genomic DNA Translation: AAB66897.1 Z48758 Genomic DNA Translation: CAA88664.1 BK006938 Genomic DNA Translation: DAA11956.1 |
PIRi | S52676 |
RefSeqi | NP_010395.1, NM_001180418.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 32168, 112 interactors |
DIPi | DIP-870N |
IntActi | O13329, 35 interactors |
MINTi | O13329 |
STRINGi | 4932.YDR110W |
PTM databases
iPTMneti | O13329 |
Proteomic databases
MaxQBi | O13329 |
PaxDbi | O13329 |
PRIDEi | O13329 |
Genome annotation databases
EnsemblFungii | YDR110W_mRNA; YDR110W; YDR110W |
GeneIDi | 851688 |
KEGGi | sce:YDR110W |
Organism-specific databases
SGDi | S000002517, FOB1 |
VEuPathDBi | FungiDB:YDR110W |
Phylogenomic databases
eggNOGi | ENOG502R9VR, Eukaryota |
HOGENOMi | CLU_030376_1_0_1 |
InParanoidi | O13329 |
OMAi | FSHFANI |
Miscellaneous databases
PROi | PR:O13329 |
RNActi | O13329, protein |
Family and domain databases
MobiDBi | Search... |
Entry informationi
Entry namei | FOB1_YEAST | |
Accessioni | O13329Primary (citable) accession number: O13329 Secondary accession number(s): D6VS96, Q04587 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | October 5, 2010 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names