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Protein

DNA replication fork-blocking protein FOB1

Gene

FOB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for replication fork blocking activity at the replication fork barrier (RFB) site in rDNA and for recombination hot-spot (HOT1) activity, regulating the recombination rate and the number of rDNA copies. Binds directly to two separated sequences in the RFB.3 Publications

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 196C2H2-type1 PublicationAdd BLAST38

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • rDNA spacer replication fork barrier binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA recombination, DNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29712-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication fork-blocking protein FOB11 Publication
Gene namesi
Name:FOB11 Publication
Synonyms:HRM11 Publication
Ordered Locus Names:YDR110WImported
ORF Names:YD9727.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR110W
SGDiS000002517 FOB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159H → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication1
Mutagenesisi164H → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication1
Mutagenesisi193C → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication1
Mutagenesisi196C → A: Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate. 1 Publication1
Mutagenesisi291D → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000873231 – 566DNA replication fork-blocking protein FOB1Add BLAST566

Proteomic databases

MaxQBiO13329
PaxDbiO13329
PRIDEiO13329

PTM databases

iPTMnetiO13329

Interactioni

Subunit structurei

Interacts with NSI1.1 Publication

Protein-protein interaction databases

BioGridi32168, 104 interactors
DIPiDIP-870N
IntActiO13329, 35 interactors
MINTiO13329
STRINGi4932.YDR110W

Structurei

3D structure databases

ProteinModelPortaliO13329
SMRiO13329
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 196C2H2-type1 PublicationAdd BLAST38

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiO13329
KOiK12577
OMAiLDRQDMF
OrthoDBiEOG092C4G5Z

Sequencei

Sequence statusi: Complete.

O13329-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS
60 70 80 90 100
MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT
110 120 130 140 150
ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH
160 170 180 190 200
VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG
210 220 230 240 250
KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR
260 270 280 290 300
SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE
310 320 330 340 350
EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG
360 370 380 390 400
PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM
410 420 430 440 450
AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD
460 470 480 490 500
LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP
510 520 530 540 550
MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ
560
QGNGDQTRDF GTSMEL
Length:566
Mass (Da):65,318
Last modified:October 5, 2010 - v2
Checksum:i2DC064120C5E7331
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103T → A in AAB66897 (PubMed:9078378).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013245 Genomic DNA Translation: AAB66897.1
Z48758 Genomic DNA Translation: CAA88664.1
BK006938 Genomic DNA Translation: DAA11956.1
PIRiS52676
RefSeqiNP_010395.1, NM_001180418.1

Genome annotation databases

EnsemblFungiiYDR110W; YDR110W; YDR110W
GeneIDi851688
KEGGisce:YDR110W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013245 Genomic DNA Translation: AAB66897.1
Z48758 Genomic DNA Translation: CAA88664.1
BK006938 Genomic DNA Translation: DAA11956.1
PIRiS52676
RefSeqiNP_010395.1, NM_001180418.1

3D structure databases

ProteinModelPortaliO13329
SMRiO13329
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32168, 104 interactors
DIPiDIP-870N
IntActiO13329, 35 interactors
MINTiO13329
STRINGi4932.YDR110W

PTM databases

iPTMnetiO13329

Proteomic databases

MaxQBiO13329
PaxDbiO13329
PRIDEiO13329

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR110W; YDR110W; YDR110W
GeneIDi851688
KEGGisce:YDR110W

Organism-specific databases

EuPathDBiFungiDB:YDR110W
SGDiS000002517 FOB1

Phylogenomic databases

InParanoidiO13329
KOiK12577
OMAiLDRQDMF
OrthoDBiEOG092C4G5Z

Enzyme and pathway databases

BioCyciYEAST:G3O-29712-MONOMER

Miscellaneous databases

PROiPR:O13329

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiFOB1_YEAST
AccessioniPrimary (citable) accession number: O13329
Secondary accession number(s): D6VS96, Q04587
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 5, 2010
Last modified: October 10, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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