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UniProtKB - O13306 (ERG1_CANGA)
Protein
Squalene monooxygenase
Gene
ERG1
Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Functioni
Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.
By similarityCatalytic activityi
- O2 + reduced [NADPH—hemoprotein reductase] + squalene = (S)-2,3-epoxysqualene + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.17By similarity
Cofactori
FADBy similarity
: lanosterol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.By similarity This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 49 | FADBy similarity | 1 | |
Sitei | 83 | Important for enzyme activityBy similarity | 1 | |
Binding sitei | 151 | FADBy similarity | 1 | |
Binding sitei | 167 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 328 | FADBy similarity | 1 | |
Binding sitei | 341 | FAD; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 21 – 22 | FADBy similarity | 2 | |
Nucleotide bindingi | 41 – 42 | FADBy similarity | 2 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- squalene monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- ergosterol biosynthetic process Source: EnsemblFungi
- ergosterol metabolic process Source: CGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00752 |
Names & Taxonomyi
Protein namesi | Recommended name: Squalene monooxygenase (EC:1.14.14.17By similarity)Alternative name(s): Squalene epoxidase Short name: SE |
Gene namesi | Name:ERG1 Ordered Locus Names:CAGL0D05940g |
Organismi | Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) |
Taxonomic identifieri | 284593 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › Nakaseomyces/Candida clade › |
Proteomesi |
|
Organism-specific databases
CGDi | CAL0128393, ERG1 |
VEuPathDBi | FungiDB:CAGL0D05940g |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Multi-pass membrane protein By similarity
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- lipid droplet Source: EnsemblFungi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 464 – 484 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209849 | 1 – 489 | Squalene monooxygenaseAdd BLAST | 489 |
Family & Domainsi
Sequence similaritiesi
Belongs to the squalene monooxygenase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1298, Eukaryota |
HOGENOMi | CLU_026390_0_0_1 |
InParanoidi | O13306 |
OMAi | KSKFWGL |
Family and domain databases
Gene3Di | 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
O13306-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLTNADETV TYDALIVGAG VIGPCVATAL ARKGKKVLIV EREWSQPDRI
60 70 80 90 100
VGELMQPGGL RALRSLGMIQ SINNIDAYPV TGYTVFYNGE HVDIPYPYKA
110 120 130 140 150
DLKPVEKLPG LVRDGNDKVL EDATVHKKDF EDDERERGVG LVHGRFLNNL
160 170 180 190 200
RNICAAEPNV TRLQGNVVEI LKDKKNEVVG AKVDVDSRGK VDFKAHLTFV
210 220 230 240 250
CDGIFSRFRR ELHPDHVPTV NSSFVGMSLY HAHMPHDMHG HVILGDKHMP
260 270 280 290 300
ILVYQISPEE TRILCAYNAP KVPTDLKSWM TKDVQPYIPK TLRPSFDDAL
310 320 330 340 350
AQGKFKPMAN SWLPARQNDV TGLCVIGDAL NMRHPLTGGG MTVGLNDVVL
360 370 380 390 400
LIKKIGDLDF SDREKVLDEL LDYHFERKNY DAVVNVLSIS LYSLFAADSK
410 420 430 440 450
NLKALQKGCF KYFQRGGDCV NLPVAFLAGV LPKPLLLTRV FFAVAFYTIY
460 470 480
LNMEERGFLG LPMALLEGIM ILITAIKVFT PFLVRELIG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 – 141 | GL → AF in AAB69189 (PubMed:9333053).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR380950 Genomic DNA Translation: CAG58578.1 AF006033 Genomic DNA Translation: AAB69189.1 |
RefSeqi | XP_445667.1, XM_445667.1 |
Genome annotation databases
EnsemblFungii | CAG58578; CAG58578; CAGL0D05940g |
GeneIDi | 2887028 |
KEGGi | cgr:CAGL0D05940g |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR380950 Genomic DNA Translation: CAG58578.1 AF006033 Genomic DNA Translation: AAB69189.1 |
RefSeqi | XP_445667.1, XM_445667.1 |
3D structure databases
SMRi | O13306 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 5478.XP_445667.1 |
Genome annotation databases
EnsemblFungii | CAG58578; CAG58578; CAGL0D05940g |
GeneIDi | 2887028 |
KEGGi | cgr:CAGL0D05940g |
Organism-specific databases
CGDi | CAL0128393, ERG1 |
VEuPathDBi | FungiDB:CAGL0D05940g |
Phylogenomic databases
eggNOGi | KOG1298, Eukaryota |
HOGENOMi | CLU_026390_0_0_1 |
InParanoidi | O13306 |
OMAi | KSKFWGL |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00752 |
Family and domain databases
Gene3Di | 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERG1_CANGA | |
Accessioni | O13306Primary (citable) accession number: O13306 Secondary accession number(s): Q6FVS7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 31, 2004 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families