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Protein

mRNA-capping enzyme subunit beta

Gene

CET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.1 Publication

Miscellaneous

Present with 3900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.

Cofactori

a divalent metal cationNote: Divalent metal ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei223N6-GMP-lysine intermediate1
Sitei280Essential for dimer formation1

GO - Molecular functioni

  • polynucleotide 5'-phosphatase activity Source: SGD

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: SGD
  • polynucleotide 5' dephosphorylation Source: GO_Central
  • polynucleotide dephosphorylation Source: SGD
  • positive regulation of protein localization to nucleus Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processmRNA capping, mRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:G3O-34116-MONOMER
YEAST:G3O-34116-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzyme subunit beta (EC:3.1.3.33)
Alternative name(s):
Polynucleotide 5'-triphosphatase
mRNA 5'-triphosphatase
Short name:
TPase
Gene namesi
Name:CET1
Ordered Locus Names:YPL228W
ORF Names:P1433
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006149 CET1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280D → A: Significant growth defects. 1 Publication1
Mutagenesisi520I → A: No growth. 1 Publication1
Mutagenesisi523F → A: Temperature sensitive growth phenotype. 1 Publication1
Mutagenesisi524L → A: Temperature sensitive growth phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002101192 – 549mRNA-capping enzyme subunit betaAdd BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei124PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO13297
PaxDbiO13297
PRIDEiO13297

PTM databases

iPTMnetiO13297

Interactioni

Subunit structurei

The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CEG1Q011599EBI-4473,EBI-10503

Protein-protein interaction databases

BioGridi35957, 508 interactors
ComplexPortaliCPX-580 mRNA cap methyltransferase complex
DIPiDIP-2299N
IntActiO13297, 5 interactors
MINTiO13297
STRINGi4932.YPL228W

Structurei

Secondary structure

1549
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO13297
SMRiO13297
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO13297

Family & Domainsi

Sequence similaritiesi

Belongs to the fungal TPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000067072
HOGENOMiHOG000111552
InParanoidiO13297
KOiK01098
OMAiQFIEKRH
OrthoDBiEOG092C2R8I

Family and domain databases

CDDicd07470 CYTH-like_mRNA_RTPase, 1 hit
Gene3Di3.20.100.10, 1 hit
InterProiView protein in InterPro
IPR033469 CYTH-like_dom_sf
IPR004206 mRNA_triPase_Cet1
IPR037009 mRNA_triPase_Cet1_sf
PfamiView protein in Pfam
PF02940 mRNA_triPase, 1 hit
SUPFAMiSSF55154 SSF55154, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13297-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSYTDNPPQT KRALSLDDLV NHDENEKVKL QKLSEAANGS RPFAENLESD
60 70 80 90 100
INQTETGQAA PIDNYKESTG HGSHSQKPKS RKSSNDDEET DTDDEMGASG
110 120 130 140 150
EINFDSEMDF DYDKQHRNLL SNGSPPMNDG SDANAKLEKP SDDSIHQNSK
160 170 180 190 200
SDEEQRIPKQ GNEGNIASNY ITQVPLQKQK QTEKKIAGNA VGSVVKKEEE
210 220 230 240 250
ANAAVDNIFE EKATLQSKKN NIKRDLEVLN EISASSKPSK YRNVPIWAQK
260 270 280 290 300
WKPTIKALQS INVKDLKIDP SFLNIIPDDD LTKSVQDWVY ATIYSIAPEL
310 320 330 340 350
RSFIELEMKF GVIIDAKGPD RVNPPVSSQC VFTELDAHLT PNIDASLFKE
360 370 380 390 400
LSKYIRGISE VTENTGKFSI IESQTRDSVY RVGLSTQRPR FLRMSTDIKT
410 420 430 440 450
GRVGQFIEKR HVAQLLLYSP KDSYDVKISL NLELPVPDND PPEKYKSQSP
460 470 480 490 500
ISERTKDRVS YIHNDSCTRI DITKVENHNQ NSKSRQSETT HEVELEINTP
510 520 530 540
ALLNAFDNIT NDSKEYASLI RTFLNNGTII RRKLSSLSYE IFEGSKKVM
Length:549
Mass (Da):61,850
Last modified:October 5, 2010 - v2
Checksum:i2B946CC53D66F824
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242R → K in BAA23522 (PubMed:9345280).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008799 Genomic DNA Translation: BAA23522.1
Z73584 Genomic DNA Translation: CAA97944.1
Z73583 Genomic DNA Translation: CAA97943.1
X94561 Genomic DNA Translation: CAA64259.1
BK006949 Genomic DNA Translation: DAA11208.1
PIRiS61706
RefSeqiNP_015096.1, NM_001184042.1

Genome annotation databases

EnsemblFungiiYPL228W; YPL228W; YPL228W
GeneIDi855873
KEGGisce:YPL228W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008799 Genomic DNA Translation: BAA23522.1
Z73584 Genomic DNA Translation: CAA97944.1
Z73583 Genomic DNA Translation: CAA97943.1
X94561 Genomic DNA Translation: CAA64259.1
BK006949 Genomic DNA Translation: DAA11208.1
PIRiS61706
RefSeqiNP_015096.1, NM_001184042.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8HX-ray2.00A/B/C241-549[»]
1D8IX-ray2.05A/B/C241-549[»]
3KYHX-ray3.00A/B241-549[»]
ProteinModelPortaliO13297
SMRiO13297
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35957, 508 interactors
ComplexPortaliCPX-580 mRNA cap methyltransferase complex
DIPiDIP-2299N
IntActiO13297, 5 interactors
MINTiO13297
STRINGi4932.YPL228W

PTM databases

iPTMnetiO13297

Proteomic databases

MaxQBiO13297
PaxDbiO13297
PRIDEiO13297

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL228W; YPL228W; YPL228W
GeneIDi855873
KEGGisce:YPL228W

Organism-specific databases

SGDiS000006149 CET1

Phylogenomic databases

GeneTreeiENSGT00530000067072
HOGENOMiHOG000111552
InParanoidiO13297
KOiK01098
OMAiQFIEKRH
OrthoDBiEOG092C2R8I

Enzyme and pathway databases

BioCyciMetaCyc:G3O-34116-MONOMER
YEAST:G3O-34116-MONOMER

Miscellaneous databases

EvolutionaryTraceiO13297
PROiPR:O13297

Family and domain databases

CDDicd07470 CYTH-like_mRNA_RTPase, 1 hit
Gene3Di3.20.100.10, 1 hit
InterProiView protein in InterPro
IPR033469 CYTH-like_dom_sf
IPR004206 mRNA_triPase_Cet1
IPR037009 mRNA_triPase_Cet1_sf
PfamiView protein in Pfam
PF02940 mRNA_triPase, 1 hit
SUPFAMiSSF55154 SSF55154, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCET1_YEAST
AccessioniPrimary (citable) accession number: O13297
Secondary accession number(s): D6W3E2, Q12197
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 5, 2010
Last modified: November 7, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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