Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ephrin type-A receptor 3

Gene

epha3

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially efna5. Upon activation by efna5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation probably through activation by efna1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei658ATPPROSITE-ProRule annotation1
Active sitei751Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi633 – 638ATPPROSITE-ProRule annotation6
Nucleotide bindingi705 – 711ATPPROSITE-ProRule annotation7
Nucleotide bindingi755 – 756ATPPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Receptor, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1 928

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-A receptor 3 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 1
Tyrosine-protein kinase receptor ZEK1
Gene namesi
Name:epha3
Synonyms:ek1, zek1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-990415-58 ek1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 545ExtracellularSequence analysisAdd BLAST525
Transmembranei546 – 566HelicalSequence analysisAdd BLAST21
Topological domaini567 – 981CytoplasmicSequence analysisAdd BLAST415

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000001680621 – 981Ephrin type-A receptor 3Add BLAST961

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi340N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi435N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi485N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei601Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei607Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei706Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei784Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei927Phosphotyrosine; by autocatalysisSequence analysis1

Post-translational modificationi

Autophosphorylates upon activation by efna5.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO13146

Expressioni

Tissue specificityi

Widely expressed in the developing zebrafish nervous system.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096552

Structurei

3D structure databases

ProteinModelPortaliO13146
SMRiO13146
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 210Eph LBDPROSITE-ProRule annotationAdd BLAST182
Domaini328 – 441Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST114
Domaini442 – 533Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST92
Domaini626 – 887Protein kinasePROSITE-ProRule annotationAdd BLAST262
Domaini910 – 974SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi979 – 981PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi192 – 325Cys-richAdd BLAST134

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196 Eukaryota
COG0515 LUCA
HOGENOMiHOG000233856
HOVERGENiHBG062180
InParanoidiO13146
KOiK05105
PhylomeDBiO13146

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR009030 Growth_fac_rcpt_cys_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF00041 fn3, 2 hits
PF07714 Pkinase_Tyr, 1 hit
PF00536 SAM_1, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00454 SAM, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
SSF57184 SSF57184, 1 hit
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFRIYSFL APFHILVLCQ ALRNYPDNEV TLLDSMSAPG DLGWEAYPSE
60 70 80 90 100
GWEEISVMDE RNIPMRTYQV CNVMEANQNN WLRTGLIQRE GAQRVYVEIK
110 120 130 140 150
FTLRDCNSLP GVPGTCKETF NVYYHESNNA VAAPLRHIRE SQYIKIDTIA
160 170 180 190 200
ADESFTQTDV GDRVMKLNTE VRDISGLSKR GLYLAFQDLG ACIALVSVRV
210 220 230 240 250
FYKRCPLAVL NLARFPDTVT GGDSALVEVR GTCVEDAEEL EGPRMFCSAD
260 270 280 290 300
GGWLVPIGRC VCRPGFEEVD GHCQPCRSGF YKASAMDAYC VKCPPHSYSH
310 320 330 340 350
QDKASECVCE RGFYRAESDP RSMACTRPPS APGNPISMVN ETAVTLEWSP
360 370 380 390 400
PRDSGGRGDV SYSVHCRKCS GETGASERCV PCGSGAHFNP RQFGLTHPRV
410 420 430 440 450
LVTELQPHTN YTFSVEALNG VSDLSPSPRQ LVSVNVTTSQ TVSVILKERK
460 470 480 490 500
GTDSVTLAWQ GPEPVDGTVV EYEVTYYEKN QQDQNYTVLK TKSNSMTVDG
510 520 530 540 550
LKPGTTYIFR VRARTDGGYG NYKGEIELET SHEDMLAVGD PNQQTILAIS
560 570 580 590 600
VAGGAVLLVL LVACFIVSGR RCGYIKAKQD PEEEKMQFQH GRVKLPETRT
610 620 630 640 650
YIHPHTYEDP NQAVRDFAKE IEVSNIRIER VIGAGEFGEV CSGRLRLPSK
660 670 680 690 700
REIQVAIKSL KAGYSEHQRR DFLSEASIMG QFDHPNIIRL EGVVTRCKPV
710 720 730 740 750
MIVTEYMENG SLDTFLKKHD GQFTVIQLLG MLRGIAAGMQ YLSEMNYVHR
760 770 780 790 800
DLAARNILVN RNLVCKVSDF GLSRVLEDDP EAAYTTRGGK IPIRWTAPEA
810 820 830 840 850
ITYRKFTSAS DVWSYGIVMW EVISYGERPY WEMSNQDVIK AVDEGYRLPA
860 870 880 890 900
PMDCPVVLHQ LMLDCWEKNR SDRPKFGQIV NTLDRLIRNP SSLKQLANSA
910 920 930 940 950
VWEDPVTPEA AVNTVEDWLD LIKMGQYKEH FSSAGYVTLD SVLYVSSSEL
960 970 980
DKMGVELAGH QKKILSSIQC LQAHHGTQVQ V
Length:981
Mass (Da):109,655
Last modified:July 1, 1997 - v1
Checksum:iF0B3F5218965E2C6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti141S → N. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89295 mRNA Translation: AAC60220.1
RefSeqiNP_571170.1, NM_131095.1
UniGeneiDr.75760

Genome annotation databases

GeneIDi30311
KEGGidre:30311

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEPHA3_DANRE
AccessioniPrimary (citable) accession number: O13146
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: November 22, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health