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UniProtKB - O12947 (ICMT_XENLA)
Protein
Protein-S-isoprenylcysteine O-methyltransferase
Gene
icmt
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
1 PublicationCatalytic activityi
- [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1001 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 194 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 209 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 251 | SubstrateBy similarity | 1 | |
Binding sitei | 255 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.1001 Publication)Alternative name(s): Farnesyl cysteine carboxyl methyltransferase Short name: FCMT Isoprenylcysteine carboxylmethyltransferase Prenylated protein carboxyl methyltransferase Short name: PPMT Prenylcysteine carboxyl methyltransferase Short name: pcCMT |
Gene namesi | Name:icmt |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-6068654, icmt.S |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 29 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 30 – 45 | LumenalSequence analysisAdd BLAST | 16 | |
Transmembranei | 46 – 63 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 64 – 73 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 74 – 91 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 92 – 96 | LumenalSequence analysis | 5 | |
Transmembranei | 97 – 116 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 117 – 135 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 136 – 153 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 154 – 158 | LumenalSequence analysis | 5 | |
Transmembranei | 159 – 178 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 179 – 216 | CytoplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 217 – 232 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 233 | LumenalSequence analysis | 1 | |
Transmembranei | 234 – 248 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 249 – 288 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209897 | 1 – 288 | Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST | 288 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 201 – 204 | S-adenosyl-L-methionine bindingBy similarity | 4 | |
Regioni | 214 – 217 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
OMAi | FFYWGLG |
OrthoDBi | 1479599at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
i Sequence
Sequence statusi: Complete.
O12947-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGARLLQEG RVSIVSFTLG ASVISLPLLT SSFTEQTLLA AAPGRIALVF
60 70 80 90 100
FIAALNGLLL LLYKAQLYQV AIRASFLGFA FGCGLLLSIT QSPWKPFGWY
110 120 130 140 150
VCSLSFFHYS EYLVTAMNNP RSLSIDSFLL NHSLEYTLAA LSSWVEFTIE
160 170 180 190 200
TTIYPDLKQI TWLSVIGLIM VLFGEVLRKC AMLTAGSNFN HIVQNEKSDS
210 220 230 240 250
HTLVTSGVYS WFRHPSYVGW FYWSIGTQVL LCNPLCLVGY TLASWRFFSE
260 270 280
RIEEEEFSLI HFFGENYLEY KKKVPTGLPF IKGVKMEP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87750 mRNA Translation: BAA19000.1 |
RefSeqi | NP_001081219.1, NM_001087750.1 |
Genome annotation databases
GeneIDi | 397717 |
KEGGi | xla:397717 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87750 mRNA Translation: BAA19000.1 |
RefSeqi | NP_001081219.1, NM_001087750.1 |
3D structure databases
SMRi | O12947 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 397717 |
KEGGi | xla:397717 |
Organism-specific databases
CTDi | 397717 |
Xenbasei | XB-GENE-6068654, icmt.S |
Phylogenomic databases
OMAi | FFYWGLG |
OrthoDBi | 1479599at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ICMT_XENLA | |
Accessioni | O12947Primary (citable) accession number: O12947 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | July 1, 1997 | |
Last modified: | September 29, 2021 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |