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Protein

Glutamate--cysteine ligase regulatory subunit

Gene

Gclm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (Gclm), Glutamate--cysteine ligase catalytic subunit (Gclc)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • enzyme regulator activity Source: GO_Central
  • glutamate-cysteine ligase activity Source: MGI
  • glutamate-cysteine ligase catalytic subunit binding Source: MGI
  • protein heterodimerization activity Source: MGI

GO - Biological processi

Keywordsi

Biological processGlutathione biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-174403 Glutathione synthesis and recycling
UniPathwayi
UPA00142;UER00209

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase regulatory subunit
Alternative name(s):
GCS light chain
Gamma-ECS regulatory subunit
Gamma-glutamylcysteine synthetase regulatory subunit
Glutamate--cysteine ligase modifier subunit
Gene namesi
Name:Gclm
Synonyms:Glclr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104995 Gclm

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925741 – 274Glutamate--cysteine ligase regulatory subunitAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineCombined sources1
Modified residuei263N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO09172
PaxDbiO09172
PeptideAtlasiO09172
PRIDEiO09172

PTM databases

iPTMnetiO09172
PhosphoSitePlusiO09172
SwissPalmiO09172

Expressioni

Gene expression databases

BgeeiENSMUSG00000028124 Expressed in 288 organ(s), highest expression level in blood
CleanExiMM_GCLM
ExpressionAtlasiO09172 baseline and differential
GenevisibleiO09172 MM

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199941, 1 interactor
IntActiO09172, 1 interactor
STRINGi10090.ENSMUSP00000029769

Structurei

3D structure databases

ProteinModelPortaliO09172
SMRiO09172
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3023 Eukaryota
COG0656 LUCA
GeneTreeiENSGT00510000047658
HOGENOMiHOG000007111
HOVERGENiHBG005923
InParanoidiO09172
KOiK11205
OMAiEYLQPYW
OrthoDBiEOG091G0DYU
PhylomeDBiO09172
TreeFamiTF105986

Family and domain databases

Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR032963 Gclm
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR13295 PTHR13295, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
SUPFAMiSSF51430 SSF51430, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O09172-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLVREFPDVL ECTMSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRSAV DMACSVLGVA QLDSVIMASP PIEDGVNLSL EHLQPYWEEL
160 170 180 190 200
ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIEAQ DWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RRGS
Length:274
Mass (Da):30,535
Last modified:July 1, 1997 - v1
Checksum:iE1D591CAB71CEA11
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BJA3H3BJA3_MOUSE
Glutamate--cysteine ligase regulato...
Gclm
199Annotation score:
F6VNW5F6VNW5_MOUSE
Glutamate--cysteine ligase regulato...
Gclm
153Annotation score:
A0A0G2JDI4A0A0G2JDI4_MOUSE
Glutamate--cysteine ligase regulato...
Gclm
98Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95053 mRNA Translation: AAB96893.1
AF149060
, AF149054, AF149055, AF149056, AF149057, AF149058, AF149059 Genomic DNA Translation: AAG15424.1
AK028236 mRNA Translation: BAC25831.1
CCDSiCCDS17808.1
RefSeqiNP_032155.1, NM_008129.4
UniGeneiMm.292676

Genome annotation databases

EnsembliENSMUST00000029769; ENSMUSP00000029769; ENSMUSG00000028124
ENSMUST00000178826; ENSMUSP00000136987; ENSMUSG00000028124
GeneIDi14630
KEGGimmu:14630
UCSCiuc008reo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95053 mRNA Translation: AAB96893.1
AF149060
, AF149054, AF149055, AF149056, AF149057, AF149058, AF149059 Genomic DNA Translation: AAG15424.1
AK028236 mRNA Translation: BAC25831.1
CCDSiCCDS17808.1
RefSeqiNP_032155.1, NM_008129.4
UniGeneiMm.292676

3D structure databases

ProteinModelPortaliO09172
SMRiO09172
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199941, 1 interactor
IntActiO09172, 1 interactor
STRINGi10090.ENSMUSP00000029769

PTM databases

iPTMnetiO09172
PhosphoSitePlusiO09172
SwissPalmiO09172

Proteomic databases

EPDiO09172
PaxDbiO09172
PeptideAtlasiO09172
PRIDEiO09172

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029769; ENSMUSP00000029769; ENSMUSG00000028124
ENSMUST00000178826; ENSMUSP00000136987; ENSMUSG00000028124
GeneIDi14630
KEGGimmu:14630
UCSCiuc008reo.2 mouse

Organism-specific databases

CTDi2730
MGIiMGI:104995 Gclm

Phylogenomic databases

eggNOGiKOG3023 Eukaryota
COG0656 LUCA
GeneTreeiENSGT00510000047658
HOGENOMiHOG000007111
HOVERGENiHBG005923
InParanoidiO09172
KOiK11205
OMAiEYLQPYW
OrthoDBiEOG091G0DYU
PhylomeDBiO09172
TreeFamiTF105986

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00209

ReactomeiR-MMU-174403 Glutathione synthesis and recycling

Miscellaneous databases

ChiTaRSiGclm mouse
PROiPR:O09172
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028124 Expressed in 288 organ(s), highest expression level in blood
CleanExiMM_GCLM
ExpressionAtlasiO09172 baseline and differential
GenevisibleiO09172 MM

Family and domain databases

Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR032963 Gclm
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR13295 PTHR13295, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
SUPFAMiSSF51430 SSF51430, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSH0_MOUSE
AccessioniPrimary (citable) accession number: O09172
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: July 1, 1997
Last modified: October 10, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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