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Protein

NFATC2-interacting protein

Gene

Nfatc2ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression. Down-regulates formation of poly-SUMO chains by UBE2I/UBC9.2 Publications

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
NFATC2-interacting protein
Alternative name(s):
45 kDa NF-AT-interacting protein
Short name:
45 kDa NFAT-interacting protein
Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein
Gene namesi
Name:Nfatc2ip
Synonyms:Nip45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1329015 Nfatc2ip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at the expected Mendelian ratio and appear healthy and viable. No alteration in thymic T-cell populations, T-cell proliferation, or peripheral lymphocyte development. Inefficient type-2 antiparasitic immune response to the intestinal nematode Trichinella spiralis due to impaired IL4 and IL13 cytokine production by Th2 cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002810091 – 412NFATC2-interacting proteinAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei79PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei118PhosphoserineBy similarity1
Cross-linki120Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei191PhosphoserineCombined sources1
Modified residuei197PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei309PhosphothreonineBy similarity1
Modified residuei311PhosphothreonineBy similarity1
Modified residuei362PhosphoserineCombined sources1
Modified residuei383PhosphoserineBy similarity1

Post-translational modificationi

Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.1 Publication

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO09130
MaxQBiO09130
PaxDbiO09130
PeptideAtlasiO09130
PRIDEiO09130

PTM databases

iPTMnetiO09130
PhosphoSitePlusiO09130

Expressioni

Tissue specificityi

Highest level detected in spleen, thymus and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000030722 Expressed in 246 organ(s), highest expression level in blastocyst
ExpressionAtlasiO09130 baseline and differential
GenevisibleiO09130 MM

Interactioni

Subunit structurei

Interacts with NFATC2, TRAF1, TRAF2 and PRMT1. Interacts with UBE2I/UBC9.4 Publications

Protein-protein interaction databases

BioGridi201740, 2 interactors
CORUMiO09130
IntActiO09130, 1 interactor
MINTiO09130
STRINGi10090.ENSMUSP00000075094

Structurei

Secondary structure

1412
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO09130
SMRiO09130
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO09130

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini341 – 412Ubiquitin-likePROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 227Sequence analysisAdd BLAST60

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1769 Eukaryota
COG5227 LUCA
GeneTreeiENSGT00390000007119
HOGENOMiHOG000285948
HOVERGENiHBG082020
InParanoidiO09130
OMAiLHLIPDH
OrthoDBiEOG091G0KS7
PhylomeDBiO09130
TreeFamiTF328600

Family and domain databases

InterProiView protein in InterPro
IPR022617 Rad60/SUMO-like_dom
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF11976 Rad60-SLD, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O09130-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEPLRGRGP RSRGGRGARR ARGARGRCPR ARQSPARLIP DTVLVDLVSD
60 70 80 90 100
SDEEVLEVAD PVEVPVARLP APAKPEQDSD SDSEGAAEGP AGAPRTLVRR
110 120 130 140 150
RRRRLLDPGE APVVPVYSGK VQSSLNLIPD NSSLLKLCPS EPEDEADLTN
160 170 180 190 200
SGSSPSEDDA LPSGSPWRKK LRKKCEKEEK KMEEFPDQDI SPLPQPSSRN
210 220 230 240 250
KSRKHTEALQ KLREVNKRLQ DLRSCLSPKQ HQSPALQSTD DEVVLVEGPV
260 270 280 290 300
LPQSSRLFTL KIRCRADLVR LPVRMSEPLQ NVVDHMANHL GVSPNRILLL
310 320 330 340 350
FGESELSPTA TPSTLKLGVA DIIDCVVLAS SSEATETSQE LRLRVQGKEK
360 370 380 390 400
HQMLEISLSP DSPLKVLMSH YEEAMGLSGH KLSFFFDGTK LSGKELPADL
410
GLESGDLIEV WG
Length:412
Mass (Da):45,121
Last modified:July 1, 1997 - v1
Checksum:iDD58FE5C7055C186
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RNY9A0A0U1RNY9_MOUSE
NFATC2-interacting protein
Nfatc2ip
110Annotation score:

Sequence cautioni

The sequence BAB24331 differs from that shown. Reason: Frameshift at position 21.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → G in BAC26788 (PubMed:16141072).Curated1
Sequence conflicti200N → D in BAC26788 (PubMed:16141072).Curated1
Sequence conflicti390 – 391KL → NS in BAB24331 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76759 mRNA Translation: AAC52963.1
AK005947 mRNA Translation: BAB24331.1 Frameshift.
AK030107 mRNA Translation: BAC26788.1
BC113761 mRNA Translation: AAI13762.1
CCDSiCCDS21827.1
RefSeqiNP_035030.2, NM_010900.3
UniGeneiMm.1389

Genome annotation databases

EnsembliENSMUST00000075671; ENSMUSP00000075094; ENSMUSG00000030722
GeneIDi18020
KEGGimmu:18020
UCSCiuc009jra.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76759 mRNA Translation: AAC52963.1
AK005947 mRNA Translation: BAB24331.1 Frameshift.
AK030107 mRNA Translation: BAC26788.1
BC113761 mRNA Translation: AAI13762.1
CCDSiCCDS21827.1
RefSeqiNP_035030.2, NM_010900.3
UniGeneiMm.1389

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A4RX-ray1.00A/B339-412[»]
3A4SX-ray2.70C/D339-412[»]
ProteinModelPortaliO09130
SMRiO09130
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201740, 2 interactors
CORUMiO09130
IntActiO09130, 1 interactor
MINTiO09130
STRINGi10090.ENSMUSP00000075094

PTM databases

iPTMnetiO09130
PhosphoSitePlusiO09130

Proteomic databases

EPDiO09130
MaxQBiO09130
PaxDbiO09130
PeptideAtlasiO09130
PRIDEiO09130

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075671; ENSMUSP00000075094; ENSMUSG00000030722
GeneIDi18020
KEGGimmu:18020
UCSCiuc009jra.1 mouse

Organism-specific databases

CTDi84901
MGIiMGI:1329015 Nfatc2ip

Phylogenomic databases

eggNOGiKOG1769 Eukaryota
COG5227 LUCA
GeneTreeiENSGT00390000007119
HOGENOMiHOG000285948
HOVERGENiHBG082020
InParanoidiO09130
OMAiLHLIPDH
OrthoDBiEOG091G0KS7
PhylomeDBiO09130
TreeFamiTF328600

Miscellaneous databases

EvolutionaryTraceiO09130
PROiPR:O09130
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030722 Expressed in 246 organ(s), highest expression level in blastocyst
ExpressionAtlasiO09130 baseline and differential
GenevisibleiO09130 MM

Family and domain databases

InterProiView protein in InterPro
IPR022617 Rad60/SUMO-like_dom
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF11976 Rad60-SLD, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNF2IP_MOUSE
AccessioniPrimary (citable) accession number: O09130
Secondary accession number(s): Q8CDG2, Q9CVY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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