UniProtKB - O09118 (NET1_MOUSE)
Protein
Netrin-1
Gene
Ntn1
Organism
Mus musculus (Mouse)
Status
Functioni
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).By similarity3 Publications
GO - Molecular functioni
- cytokine activity Source: UniProtKB
GO - Biological processi
- animal organ morphogenesis Source: GO_Central
- anterior/posterior axon guidance Source: MGI
- apoptotic process Source: UniProtKB-KW
- axon guidance Source: MGI
- axonogenesis Source: MGI
- B cell mediated immunity Source: UniProtKB
- B cell proliferation Source: UniProtKB
- Cdc42 protein signal transduction Source: UniProtKB
- cell-cell adhesion Source: MGI
- chemorepulsion of axon Source: UniProtKB
- dendrite development Source: GO_Central
- inner ear morphogenesis Source: MGI
- mammary gland development Source: MGI
- mammary gland duct morphogenesis Source: MGI
- motor neuron axon guidance Source: GO_Central
- negative regulation of axon extension Source: MGI
- neuron migration Source: MGI
- nuclear migration Source: MGI
- positive regulation of axon extension Source: MGI
- positive regulation of cell motility Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- Ras protein signal transduction Source: UniProtKB
- regulation of cell migration Source: MGI
- regulation of synapse assembly Source: SynGO
- substrate-dependent cell migration, cell extension Source: UniProtKB
- T cell mediated immunity Source: UniProtKB
- tissue development Source: GO_Central
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-373752, Netrin-1 signaling R-MMU-418885, DCC mediated attractive signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Netrin-1 |
Gene namesi | Name:Ntn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105088, Ntn1 |
Subcellular locationi
Extracellular region or secreted
- extracellular region Source: UniProtKB
Other locations
- basement membrane Source: MGI
- cell periphery Source: MGI
- collagen-containing extracellular matrix Source: BHF-UCL
- cytoplasm Source: MGI
- extracellular matrix Source: MGI
Keywords - Cellular componenti
Cytoplasm, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000017083 | 25 – 604 | Netrin-1Add BLAST | 580 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 95 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 116 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 119 ↔ 152 | By similarity | ||
Glycosylationi | 131 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 285 ↔ 294 | By similarity | ||
Disulfide bondi | 287 ↔ 304 | By similarity | ||
Disulfide bondi | 306 ↔ 315 | By similarity | ||
Disulfide bondi | 318 ↔ 338 | By similarity | ||
Disulfide bondi | 341 ↔ 350 | By similarity | ||
Disulfide bondi | 343 ↔ 368 | By similarity | ||
Disulfide bondi | 371 ↔ 380 | By similarity | ||
Disulfide bondi | 383 ↔ 401 | By similarity | ||
Disulfide bondi | 404 ↔ 416 | By similarity | ||
Disulfide bondi | 406 ↔ 423 | By similarity | ||
Glycosylationi | 417 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 425 ↔ 434 | By similarity | ||
Disulfide bondi | 437 ↔ 451 | By similarity | ||
Disulfide bondi | 472 ↔ 544 | By similarity | ||
Disulfide bondi | 491 ↔ 601 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | O09118 |
PaxDbi | O09118 |
PeptideAtlasi | O09118 |
PRIDEi | O09118 |
ProteomicsDBi | 287480 |
PTM databases
GlyConnecti | 2531, 1 N-Linked glycan (1 site) |
GlyGeni | O09118, 4 sites |
PhosphoSitePlusi | O09118 |
Expressioni
Tissue specificityi
In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020902, Expressed in aorta and 298 other tissues |
ExpressionAtlasi | O09118, baseline and differential |
Genevisiblei | O09118, MM |
Interactioni
Subunit structurei
Binary interactionsi
O09118
With | #Exp. | IntAct |
---|---|---|
Unc5b - isoform 1 [Q8K1S3-1] | 4 | EBI-1798844,EBI-11658250 |
GO - Molecular functioni
- cytokine activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 201866, 7 interactors |
IntActi | O09118, 4 interactors |
STRINGi | 10090.ENSMUSP00000104314 |
Miscellaneous databases
RNActi | O09118, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O09118 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 47 – 284 | Laminin N-terminalPROSITE-ProRule annotationAdd BLAST | 238 | |
Domaini | 285 – 340 | Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 341 – 403 | Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 404 – 453 | Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 472 – 601 | NTRPROSITE-ProRule annotationAdd BLAST | 130 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 530 – 532 | Cell attachment siteSequence analysis | 3 |
Keywords - Domaini
Laminin EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG3512, Eukaryota |
GeneTreei | ENSGT00940000153882 |
HOGENOMi | CLU_018213_2_0_1 |
InParanoidi | O09118 |
OMAi | WGRATAN |
OrthoDBi | 858946at2759 |
TreeFami | TF352481 |
Family and domain databases
Gene3Di | 2.40.50.120, 1 hit 2.60.120.1490, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR002049, Laminin_EGF IPR008211, Laminin_N IPR038684, Laminin_N_sf IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00053, Laminin_EGF, 3 hits PF00055, Laminin_N, 1 hit PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00180, EGF_Lam, 3 hits SM00136, LamNT, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01248, EGF_LAM_1, 3 hits PS50027, EGF_LAM_2, 3 hits PS51117, LAMININ_NTER, 1 hit PS50189, NTR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O09118-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR
60 70 80 90 100
CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK
110 120 130 140 150
KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ
160 170 180 190 200
FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE
210 220 230 240 250
AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA
260 270 280 290 300
FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD
310 320 330 340 350
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC
360 370 380 390 400
RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA
410 420 430 440 450
CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP
460 470 480 490 500
CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH
510 520 530 540 550
ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK
560 570 580 590 600
PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK
CKKA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB0QZL0 | B0QZL0_MOUSE | Netrin-1 | Ntn1 | 159 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | Missing in AAC52971 (PubMed:8978605).Curated | 1 | |
Sequence conflicti | 88 | L → V in AAC52971 (PubMed:8978605).Curated | 1 | |
Sequence conflicti | 88 | L → V in AAD28602 (PubMed:10381568).Curated | 1 | |
Sequence conflicti | 466 | V → A in AAD28602 (PubMed:10381568).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65418 mRNA Translation: AAC52971.1 AF128865 mRNA Translation: AAD28602.1 AL606831 Genomic DNA No translation available. AL662894 Genomic DNA No translation available. AL669842 Genomic DNA No translation available. CH466601 Genomic DNA Translation: EDL10446.1 BC141294 mRNA Translation: AAI41295.1 U23505 mRNA Translation: AAA87938.1 |
CCDSi | CCDS24864.1 |
RefSeqi | NP_032770.2, NM_008744.2 |
Genome annotation databases
Ensembli | ENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902 ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902 |
GeneIDi | 18208 |
KEGGi | mmu:18208 |
UCSCi | uc007jnm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65418 mRNA Translation: AAC52971.1 AF128865 mRNA Translation: AAD28602.1 AL606831 Genomic DNA No translation available. AL662894 Genomic DNA No translation available. AL669842 Genomic DNA No translation available. CH466601 Genomic DNA Translation: EDL10446.1 BC141294 mRNA Translation: AAI41295.1 U23505 mRNA Translation: AAA87938.1 |
CCDSi | CCDS24864.1 |
RefSeqi | NP_032770.2, NM_008744.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OVE | X-ray | 2.64 | A | 23-457 | [»] | |
SMRi | O09118 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201866, 7 interactors |
IntActi | O09118, 4 interactors |
STRINGi | 10090.ENSMUSP00000104314 |
PTM databases
GlyConnecti | 2531, 1 N-Linked glycan (1 site) |
GlyGeni | O09118, 4 sites |
PhosphoSitePlusi | O09118 |
Proteomic databases
MaxQBi | O09118 |
PaxDbi | O09118 |
PeptideAtlasi | O09118 |
PRIDEi | O09118 |
ProteomicsDBi | 287480 |
Protocols and materials databases
Antibodypediai | 24762, 350 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902 ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902 |
GeneIDi | 18208 |
KEGGi | mmu:18208 |
UCSCi | uc007jnm.1, mouse |
Organism-specific databases
CTDi | 9423 |
MGIi | MGI:105088, Ntn1 |
Phylogenomic databases
eggNOGi | KOG3512, Eukaryota |
GeneTreei | ENSGT00940000153882 |
HOGENOMi | CLU_018213_2_0_1 |
InParanoidi | O09118 |
OMAi | WGRATAN |
OrthoDBi | 858946at2759 |
TreeFami | TF352481 |
Enzyme and pathway databases
Reactomei | R-MMU-373752, Netrin-1 signaling R-MMU-418885, DCC mediated attractive signaling |
Miscellaneous databases
BioGRID-ORCSi | 18208, 0 hits in 52 CRISPR screens |
ChiTaRSi | Ntn1, mouse |
PROi | PR:O09118 |
RNActi | O09118, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020902, Expressed in aorta and 298 other tissues |
ExpressionAtlasi | O09118, baseline and differential |
Genevisiblei | O09118, MM |
Family and domain databases
Gene3Di | 2.40.50.120, 1 hit 2.60.120.1490, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR002049, Laminin_EGF IPR008211, Laminin_N IPR038684, Laminin_N_sf IPR001134, Netrin_domain IPR018933, Netrin_module_non-TIMP IPR008993, TIMP-like_OB-fold |
Pfami | View protein in Pfam PF00053, Laminin_EGF, 3 hits PF00055, Laminin_N, 1 hit PF01759, NTR, 1 hit |
SMARTi | View protein in SMART SM00643, C345C, 1 hit SM00180, EGF_Lam, 3 hits SM00136, LamNT, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF50242, SSF50242, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01248, EGF_LAM_1, 3 hits PS50027, EGF_LAM_2, 3 hits PS51117, LAMININ_NTER, 1 hit PS50189, NTR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NET1_MOUSE | |
Accessioni | O09118Primary (citable) accession number: O09118 Secondary accession number(s): B1ARR0, Q60832, Q9QY50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references