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Protein

Netrin-1

Gene

Ntn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).By similarity2 Publications

GO - Biological processi

  • anterior/posterior axon guidance Source: MGI
  • apoptotic process Source: UniProtKB-KW
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • Cdc42 protein signal transduction Source: UniProtKB
  • cell-cell adhesion Source: MGI
  • inner ear morphogenesis Source: MGI
  • mammary gland development Source: MGI
  • mammary gland duct morphogenesis Source: MGI
  • negative regulation of axon extension Source: MGI
  • neuron migration Source: MGI
  • nuclear migration Source: MGI
  • positive regulation of axon extension Source: MGI
  • positive regulation of cell motility Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • Ras protein signal transduction Source: UniProtKB
  • regulation of cell migration Source: MGI
  • substrate-dependent cell migration, cell extension Source: UniProtKB

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-373752 Netrin-1 signaling
R-MMU-418885 DCC mediated attractive signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-1
Gene namesi
Name:Ntn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105088 Ntn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001708325 – 604Netrin-1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi119 ↔ 152By similarity
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi285 ↔ 294By similarity
Disulfide bondi287 ↔ 304By similarity
Disulfide bondi306 ↔ 315By similarity
Disulfide bondi318 ↔ 338By similarity
Disulfide bondi341 ↔ 350By similarity
Disulfide bondi343 ↔ 368By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi383 ↔ 401By similarity
Disulfide bondi404 ↔ 416By similarity
Disulfide bondi406 ↔ 423By similarity
Glycosylationi417N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi425 ↔ 434By similarity
Disulfide bondi437 ↔ 451By similarity
Disulfide bondi472 ↔ 544By similarity
Disulfide bondi491 ↔ 601By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO09118
PaxDbiO09118
PeptideAtlasiO09118
PRIDEiO09118

PTM databases

PhosphoSitePlusiO09118

Expressioni

Tissue specificityi

In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000020902
CleanExiMM_NTN1
ExpressionAtlasiO09118 baseline and differential
GenevisibleiO09118 MM

Interactioni

Subunit structurei

Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D (By similarity). Binds to its receptor; DSCAM.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Unc5bQ8K1S3-14EBI-1798844,EBI-11658250

Protein-protein interaction databases

IntActiO09118, 4 interactors
STRINGi10090.ENSMUSP00000021284

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 42Combined sources3
Turni58 – 61Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi75 – 81Combined sources7
Beta strandi83 – 85Combined sources3
Beta strandi88 – 94Combined sources7
Turni99 – 101Combined sources3
Helixi105 – 108Combined sources4
Beta strandi132 – 153Combined sources22
Beta strandi157 – 166Combined sources10
Beta strandi172 – 177Combined sources6
Helixi181 – 185Combined sources5
Beta strandi197 – 199Combined sources3
Helixi206 – 208Combined sources3
Beta strandi213 – 215Combined sources3
Beta strandi217 – 221Combined sources5
Turni222 – 225Combined sources4
Helixi227 – 234Combined sources8
Helixi236 – 241Combined sources6
Beta strandi243 – 253Combined sources11
Beta strandi275 – 285Combined sources11
Beta strandi294 – 296Combined sources3
Beta strandi302 – 304Combined sources3
Turni312 – 315Combined sources4
Beta strandi348 – 352Combined sources5
Helixi354 – 359Combined sources6
Beta strandi366 – 370Combined sources5
Beta strandi373 – 376Combined sources4
Turni377 – 380Combined sources4
Beta strandi387 – 389Combined sources3
Beta strandi401 – 403Combined sources3
Turni408 – 410Combined sources3
Turni418 – 420Combined sources3
Turni431 – 434Combined sources4
Beta strandi441 – 443Combined sources3
Beta strandi451 – 453Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OVEX-ray2.64A23-457[»]
ProteinModelPortaliO09118
SMRiO09118
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 284Laminin N-terminalPROSITE-ProRule annotationAdd BLAST238
Domaini285 – 340Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST56
Domaini341 – 403Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST63
Domaini404 – 453Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST50
Domaini472 – 601NTRPROSITE-ProRule annotationAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi530 – 532Cell attachment siteSequence analysis3

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512 Eukaryota
ENOG410XS7U LUCA
GeneTreeiENSGT00890000139348
HOGENOMiHOG000286017
HOVERGENiHBG006464
InParanoidiO09118
KOiK06843
OMAiDQFLWVR
OrthoDBiEOG091G005L
TreeFamiTF352481

Family and domain databases

Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
IPR001134 Netrin_domain
IPR018933 Netrin_module_non-TIMP
IPR008993 TIMP-like_OB-fold
PfamiView protein in Pfam
PF00053 Laminin_EGF, 3 hits
PF00055 Laminin_N, 1 hit
PF01759 NTR, 1 hit
SMARTiView protein in SMART
SM00643 C345C, 1 hit
SM00180 EGF_Lam, 3 hits
SM00136 LamNT, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF50242 SSF50242, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01248 EGF_LAM_1, 3 hits
PS50027 EGF_LAM_2, 3 hits
PS51117 LAMININ_NTER, 1 hit
PS50189 NTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR
60 70 80 90 100
CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNSSDPK
110 120 130 140 150
KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ
160 170 180 190 200
FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE
210 220 230 240 250
AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA
260 270 280 290 300
FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD
310 320 330 340 350
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC
360 370 380 390 400
RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA
410 420 430 440 450
CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP
460 470 480 490 500
CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH
510 520 530 540 550
ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK
560 570 580 590 600
PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK

CKKA
Length:604
Mass (Da):67,810
Last modified:July 27, 2011 - v3
Checksum:i135120DB6F0482F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24Missing in AAC52971 (PubMed:8978605).Curated1
Sequence conflicti88L → V in AAC52971 (PubMed:8978605).Curated1
Sequence conflicti88L → V in AAD28602 (PubMed:10381568).Curated1
Sequence conflicti466V → A in AAD28602 (PubMed:10381568).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65418 mRNA Translation: AAC52971.1
AF128865 mRNA Translation: AAD28602.1
AL669842, AL606831, AL662894 Genomic DNA Translation: CAI24775.1
AL606831, AL662894, AL669842 Genomic DNA Translation: CAI25691.1
AL662894, AL606831, AL669842 Genomic DNA Translation: CAI25793.1
CH466601 Genomic DNA Translation: EDL10446.1
BC141294 mRNA Translation: AAI41295.1
U23505 mRNA Translation: AAA87938.1
CCDSiCCDS24864.1
RefSeqiNP_032770.2, NM_008744.2
UniGeneiMm.39095

Genome annotation databases

EnsembliENSMUST00000021284; ENSMUSP00000021284; ENSMUSG00000020902
ENSMUST00000108674; ENSMUSP00000104314; ENSMUSG00000020902
GeneIDi18208
KEGGimmu:18208
UCSCiuc007jnm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNET1_MOUSE
AccessioniPrimary (citable) accession number: O09118
Secondary accession number(s): B1ARR0, Q60832, Q9QY50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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