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Protein

Prostaglandin-H2 D-isomerase

Gene

Ptgds

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.8 Publications

Catalytic activityi

(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.2 Publications

Kineticsi

  1. KM=0.8 µM for prostaglandin H21 Publication
  1. Vmax=5.9 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Nucleophile1

GO - Molecular functioni

  • fatty acid binding Source: MGI
  • prostaglandin-D synthase activity Source: UniProtKB
  • retinoid binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism, Transport

Enzyme and pathway databases

BRENDAi5.3.99.2 3474
ReactomeiR-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin-H2 D-isomerase (EC:5.3.99.2)
Alternative name(s):
Glutathione-independent PGD synthase
Lipocalin-type prostaglandin-D synthase
Prostaglandin-D2 synthase
Short name:
L-PGDS
Short name:
PGD2 synthase
Short name:
PGDS2
Gene namesi
Name:Ptgds
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99261 Ptgds

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45S → A: Reduces enzyme activity by about half. Reduces enzyme activity tenfold; when associated with A-67 and A-81. 1 Publication1
Mutagenesisi65C → A: Loss of enzyme activity. No effect on ligand binding. 1 Publication1
Mutagenesisi67T → A: Reduces enzyme activity by about half. Reduces enzyme activity tenfold; when associated with A-45 and A-81. 1 Publication1
Mutagenesisi81S → A: Slightly reduced enzyme activity. half. Reduces enzyme activity tenfold; when associated with A-45 and A-67. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4334

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000001794725 – 189Prostaglandin-H2 D-isomeraseAdd BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25Pyrrolidone carboxylic acidBy similarity1
Glycosylationi51N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi78N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi89 ↔ 1861 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiO09114
PeptideAtlasiO09114
PRIDEiO09114

PTM databases

PhosphoSitePlusiO09114

Expressioni

Tissue specificityi

Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. In the male reproductive system, it is expressed in the testis, efferent ducts and epididymis, and is secreted into the seminal fluid. In the eye, it is expressed in the pigmented epithelium of the retina and the nonpigmented epithelium of the ciliary body, and secreted into the aqueous humor. Low levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including the ear, heart and lung.4 Publications

Developmental stagei

Initially detected at 14.5 dpc in the mesenchymal cells of the brain. Later in development, observed in the choroid plexus and within single cells in the brain.

Inductioni

By IL-1 beta and thyroid hormone. Probably induced by dexamethasone, dihydrotestosterone, progesterone, retinoic acid and retinal. Repressed by the Notch-Hes signaling pathway.1 Publication

Gene expression databases

BgeeiENSMUSG00000015090 Expressed in 185 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_PTGDS
GenevisibleiO09114 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015234

Chemistry databases

BindingDBiO09114

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO09114
SMRiO09114
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO09114

Family & Domainsi

Domaini

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.3 Publications

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II11 Eukaryota
ENOG4111YRI LUCA
GeneTreeiENSGT00920000149140
HOGENOMiHOG000231660
HOVERGENiHBG106490
InParanoidiO09114
KOiK01830
OMAiKGPGQDF
OrthoDBiEOG091G0NAJ
PhylomeDBiO09114
TreeFamiTF336103

Family and domain databases

Gene3Di2.40.128.20, 1 hit
InterProiView protein in InterPro
IPR012674 Calycin
IPR002345 Lipocalin
IPR022272 Lipocalin_CS
IPR000566 Lipocln_cytosolic_FA-bd_dom
IPR002972 PstgldnD_synth
PANTHERiPTHR11430 PTHR11430, 1 hit
PTHR11430:SF86 PTHR11430:SF86, 1 hit
PfamiView protein in Pfam
PF00061 Lipocalin, 1 hit
SUPFAMiSSF50814 SSF50814, 1 hit
PROSITEiView protein in PROSITE
PS00213 LIPOCALIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O09114-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAALRMLWMG LVLLGLLGFP QTPAQGHDTV QPNFQQDKFL GRWYSAGLAS
60 70 80 90 100
NSSWFREKKA VLYMCKTVVA PSTEGGLNLT STFLRKNQCE TKIMVLQPAG
110 120 130 140 150
APGHYTYSSP HSGSIHSVSV VEANYDEYAL LFSRGTKGPG QDFRMATLYS
160 170 180
RTQTLKDELK EKFTTFSKAQ GLTEEDIVFL PQPDKCIQE
Length:189
Mass (Da):21,066
Last modified:July 1, 1997 - v1
Checksum:iC2C0B3E3B5928643
GO
Isoform 2 (identifier: O09114-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: Missing.

Note: No experimental confirmation available.
Show »
Length:126
Mass (Da):13,941
Checksum:i7F9FEDCFBEFF99A6
GO

Sequence cautioni

The sequence BAA21769 differs from that shown. Reason: Frameshift at position 47.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0410291 – 63Missing in isoform 2. 1 PublicationAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89222 mRNA Translation: CAA61506.1
Y10138 Genomic DNA Translation: CAA71226.1
AB006361 mRNA Translation: BAA21769.1 Frameshift.
D83329 Genomic DNA Translation: BAA74461.1
AK131859 mRNA Translation: BAE20833.1
CH466542 Genomic DNA Translation: EDL08250.1
CH466542 Genomic DNA Translation: EDL08251.1
BC038083 mRNA Translation: AAH38083.1
BC043015 mRNA Translation: AAH43015.1
CCDSiCCDS38074.1 [O09114-1]
PIRiS57748
RefSeqiNP_032989.2, NM_008963.2 [O09114-1]
XP_006497849.1, XM_006497786.3 [O09114-1]
XP_006497850.1, XM_006497787.3 [O09114-1]
UniGeneiMm.1008

Genome annotation databases

EnsembliENSMUST00000015234; ENSMUSP00000015234; ENSMUSG00000015090 [O09114-1]
ENSMUST00000114251; ENSMUSP00000109889; ENSMUSG00000015090 [O09114-1]
ENSMUST00000114259; ENSMUSP00000109897; ENSMUSG00000015090 [O09114-1]
GeneIDi19215
KEGGimmu:19215
UCSCiuc008isf.1 mouse [O09114-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89222 mRNA Translation: CAA61506.1
Y10138 Genomic DNA Translation: CAA71226.1
AB006361 mRNA Translation: BAA21769.1 Frameshift.
D83329 Genomic DNA Translation: BAA74461.1
AK131859 mRNA Translation: BAE20833.1
CH466542 Genomic DNA Translation: EDL08250.1
CH466542 Genomic DNA Translation: EDL08251.1
BC038083 mRNA Translation: AAH38083.1
BC043015 mRNA Translation: AAH43015.1
CCDSiCCDS38074.1 [O09114-1]
PIRiS57748
RefSeqiNP_032989.2, NM_008963.2 [O09114-1]
XP_006497849.1, XM_006497786.3 [O09114-1]
XP_006497850.1, XM_006497787.3 [O09114-1]
UniGeneiMm.1008

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CZTX-ray2.00A25-189[»]
2CZUX-ray2.10A/B25-189[»]
2E4JNMR-A25-189[»]
2KTDNMR-A25-189[»]
2RQ0NMR-A25-189[»]
ProteinModelPortaliO09114
SMRiO09114
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015234

Chemistry databases

BindingDBiO09114
ChEMBLiCHEMBL4334

PTM databases

PhosphoSitePlusiO09114

Proteomic databases

PaxDbiO09114
PeptideAtlasiO09114
PRIDEiO09114

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015234; ENSMUSP00000015234; ENSMUSG00000015090 [O09114-1]
ENSMUST00000114251; ENSMUSP00000109889; ENSMUSG00000015090 [O09114-1]
ENSMUST00000114259; ENSMUSP00000109897; ENSMUSG00000015090 [O09114-1]
GeneIDi19215
KEGGimmu:19215
UCSCiuc008isf.1 mouse [O09114-1]

Organism-specific databases

CTDi5730
MGIiMGI:99261 Ptgds

Phylogenomic databases

eggNOGiENOG410II11 Eukaryota
ENOG4111YRI LUCA
GeneTreeiENSGT00920000149140
HOGENOMiHOG000231660
HOVERGENiHBG106490
InParanoidiO09114
KOiK01830
OMAiKGPGQDF
OrthoDBiEOG091G0NAJ
PhylomeDBiO09114
TreeFamiTF336103

Enzyme and pathway databases

BRENDAi5.3.99.2 3474
ReactomeiR-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Miscellaneous databases

ChiTaRSiPtgds mouse
EvolutionaryTraceiO09114
PROiPR:O09114
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015090 Expressed in 185 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_PTGDS
GenevisibleiO09114 MM

Family and domain databases

Gene3Di2.40.128.20, 1 hit
InterProiView protein in InterPro
IPR012674 Calycin
IPR002345 Lipocalin
IPR022272 Lipocalin_CS
IPR000566 Lipocln_cytosolic_FA-bd_dom
IPR002972 PstgldnD_synth
PANTHERiPTHR11430 PTHR11430, 1 hit
PTHR11430:SF86 PTHR11430:SF86, 1 hit
PfamiView protein in Pfam
PF00061 Lipocalin, 1 hit
SUPFAMiSSF50814 SSF50814, 1 hit
PROSITEiView protein in PROSITE
PS00213 LIPOCALIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTGDS_MOUSE
AccessioniPrimary (citable) accession number: O09114
Secondary accession number(s): O09157
, O35091, Q3V2G5, Q62169
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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