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Protein

Dual specificity protein phosphatase 8

Gene

Dusp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation (PubMed:7561881). Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues (Probable).Curated1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • inactivation of MAPK activity Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-112409 RAF-independent MAPK1/3 activation
R-MMU-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 8 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Neuronal tyrosine threonine phosphatase 1
Gene namesi
Name:Dusp8
Synonyms:Nttp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106626 Dusp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948111 – 663Dual specificity protein phosphatase 8Add BLAST663

Proteomic databases

MaxQBiO09112
PaxDbiO09112
PRIDEiO09112

PTM databases

iPTMnetiO09112
PhosphoSitePlusiO09112

Expressioni

Tissue specificityi

Expressed predominantly in brain and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000037887 Expressed in 217 organ(s), highest expression level in submandibular gland
CleanExiMM_DUSP8
ExpressionAtlasiO09112 baseline and differential
GenevisibleiO09112 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049414

Structurei

3D structure databases

ProteinModelPortaliO09112
SMRiO09112
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 138RhodanesePROSITE-ProRule annotationAdd BLAST116
Domaini162 – 432Tyrosine-protein phosphataseAdd BLAST271

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi311 – 552Pro-richAdd BLAST242
Compositional biasi452 – 459Poly-Arg8
Compositional biasi555 – 558Poly-Ser4
Compositional biasi559 – 576Poly-GlyAdd BLAST18
Compositional biasi577 – 600Poly-SerAdd BLAST24

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOVERGENiHBG005541
InParanoidiO09112
KOiK04459
OMAiDARTGWP
OrthoDBiEOG091G0249
TreeFamiTF105122

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O09112-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS CHVLSSVNIC
60 70 80 90 100
CSKLVKRRLQ QGKVTIAELI QPATRSQVDA TEPQDVVVYD QSTRDASVLA
110 120 130 140 150
ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PATLPSMSLS
160 170 180 190 200
QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP
210 220 230 240 250
DFICESRFMR IPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI
260 270 280 290 300
SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
310 320 330 340 350
KLLAALQTDG PHLGTPEPLM GPAAGIPLPR LPPSTSESAA TGSEAATAAR
360 370 380 390 400
EGSPSAGGDA PIPSTAPATS ALQQGLRGLH LSSDRLQDTN RLKRSFSLDI
410 420 430 440 450
KSAYAPSRRP DFPGPPDPGE APKLCKLDSP SGGTLGLPSP SPDSPDSVPE
460 470 480 490 500
CRPRPRRRRP PASSPARSPA HGLGLNFGDT ARQTPRHGLS ALSAPGLPGP
510 520 530 540 550
GQPAGPGGWV PPLDSPGTPS PDGPWCFSPE GAQGPGAVFS AFGRVSAGAP
560 570 580 590 600
GPGNSSSSGG GGGGGGGGGG GGGGGGSSSS NSSSSSSSSS SSSSSSSSSS
610 620 630 640 650
DLRRRDVRTG WPEEPAADAQ FKRRSCQMEF EEGMVEGRAR GEELAALGKQ
660
TSFSGSVEVI EVS
Length:663
Mass (Da):68,847
Last modified:July 1, 1997 - v1
Checksum:i416F429A12C1FA7C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZC52B7ZC52_MOUSE
Dual-specificity protein phosphatas...
Dusp8
231Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95518 mRNA Translation: CAA64772.1
CCDSiCCDS22024.1
RefSeqiNP_032774.1, NM_008748.3
XP_006508572.1, XM_006508509.3
XP_011240291.1, XM_011241989.2
UniGeneiMm.39725

Genome annotation databases

EnsembliENSMUST00000039926; ENSMUSP00000049414; ENSMUSG00000037887
GeneIDi18218
KEGGimmu:18218
UCSCiuc009kmo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95518 mRNA Translation: CAA64772.1
CCDSiCCDS22024.1
RefSeqiNP_032774.1, NM_008748.3
XP_006508572.1, XM_006508509.3
XP_011240291.1, XM_011241989.2
UniGeneiMm.39725

3D structure databases

ProteinModelPortaliO09112
SMRiO09112
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049414

PTM databases

iPTMnetiO09112
PhosphoSitePlusiO09112

Proteomic databases

MaxQBiO09112
PaxDbiO09112
PRIDEiO09112

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039926; ENSMUSP00000049414; ENSMUSG00000037887
GeneIDi18218
KEGGimmu:18218
UCSCiuc009kmo.2 mouse

Organism-specific databases

CTDi1850
MGIiMGI:106626 Dusp8

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOVERGENiHBG005541
InParanoidiO09112
KOiK04459
OMAiDARTGWP
OrthoDBiEOG091G0249
TreeFamiTF105122

Enzyme and pathway databases

ReactomeiR-MMU-112409 RAF-independent MAPK1/3 activation
R-MMU-5675221 Negative regulation of MAPK pathway

Miscellaneous databases

PROiPR:O09112
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037887 Expressed in 217 organ(s), highest expression level in submandibular gland
CleanExiMM_DUSP8
ExpressionAtlasiO09112 baseline and differential
GenevisibleiO09112 MM

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDUS8_MOUSE
AccessioniPrimary (citable) accession number: O09112
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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