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Entry version 185 (12 Aug 2020)
Sequence version 3 (27 Jul 2011)
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Protein

Werner syndrome ATP-dependent helicase homolog

Gene

Wrn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (By similarity).By similarity2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication, Mn2+1 Publication, Zn2+1 PublicationNote: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Zinc ions stimulate the exonuclease activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi76Magnesium 1; catalyticCombined sources1 Publication1
Metal bindingi76Magnesium 2; catalyticCombined sources1 Publication1
Metal bindingi78Magnesium 1; catalyticCombined sources1 Publication1
Metal bindingi210Magnesium 1; catalyticCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi535 – 542ATPPROSITE-ProRule annotation8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Exonuclease, Helicase, Hydrolase, Multifunctional enzyme, Nuclease
Biological processDNA damage, DNA repair
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-174414, Processive synthesis on the C-strand of the telomere
R-MMU-174437, Removal of the Flap Intermediate from the C-strand
R-MMU-5685938, HDR through Single Strand Annealing (SSA)
R-MMU-5685942, HDR through Homologous Recombination (HRR)
R-MMU-5693554, Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA)
R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579, Homologous DNA Pairing and Strand Exchange
R-MMU-5693607, Processing of DNA double-strand break ends
R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation
R-MMU-69473, G2/M DNA damage checkpoint

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Werner syndrome ATP-dependent helicase homolog (EC:3.6.4.121 Publication)
Alternative name(s):
Exonuclease WRN (EC:3.1.-.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Wrn
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 8

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:109635, Wrn

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi185K → A: Loss of exonuclease activity. 1 Publication1
Mutagenesisi190R → A: Strongly reduced exonuclease activity. 1 Publication1
Mutagenesisi206Y → F: Loss of exonuclease activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002050461 – 1401Werner syndrome ATP-dependent helicase homologAdd BLAST1401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki148Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki235Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei419PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei444PhosphoserineBy similarity1
Modified residuei1098PhosphoserineBy similarity1
Modified residuei1364PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by PRKDC.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
O09053

MaxQB - The MaxQuant DataBase

More...
MaxQBi
O09053

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O09053

PeptideAtlas

More...
PeptideAtlasi
O09053

PRoteomics IDEntifications database

More...
PRIDEi
O09053

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O09053

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
O09053

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed ubiquitously in most organs at a low level, highly expressed in testis, ovary and spleen.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000031583, Expressed in secondary oocyte and 266 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
O09053, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
O09053, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA.

Interacts via its N-terminal domain with WRNIP1.

Interacts with PCNA; EXO1 and SUPV3L1 (By similarity).

Interacts with PML (By similarity).

By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei139Interaction with DNABy similarity1
Sitei1002Interaction with DNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
204584, 7 interactors

Database of interacting proteins

More...
DIPi
DIP-27642N

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000033990

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
O09053, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O09053

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
O09053

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini51 – 2233'-5' exonucleaseAdd BLAST173
Domaini522 – 688Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167
Domaini713 – 866Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154
Domaini1115 – 1194HRDCPROSITE-ProRule annotationAdd BLAST80

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 271Interaction with WRNIP11 PublicationAdd BLAST271
Regioni952 – 958Interaction with DNABy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi632 – 635DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1387 – 1390Poly-Ser4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0351, Eukaryota
KOG4373, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000159168

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_001103_11_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O09053

KEGG Orthology (KO)

More...
KOi
K10900

Database of Orthologous Groups

More...
OrthoDBi
445763at2759

TreeFam database of animal gene trees

More...
TreeFami
TF312852

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.10, 1 hit
3.30.420.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002562, 3'-5'_exonuclease_dom
IPR011545, DEAD/DEAH_box_helicase_dom
IPR004589, DNA_helicase_ATP-dep_RecQ
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR029491, Helicase_HTH
IPR010997, HRDC-like_sf
IPR002121, HRDC_dom
IPR027417, P-loop_NTPase
IPR032284, RecQ_Zn-bd
IPR012337, RNaseH-like_sf
IPR036397, RNaseH_sf
IPR018982, RQC_domain
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00270, DEAD, 1 hit
PF01612, DNA_pol_A_exo1, 1 hit
PF00271, Helicase_C, 1 hit
PF00570, HRDC, 1 hit
PF14493, HTH_40, 1 hit
PF16124, RecQ_Zn_bind, 1 hit
PF09382, RQC, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00474, 35EXOc, 1 hit
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit
SM00341, HRDC, 1 hit
SM00956, RQC, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF46785, SSF46785, 1 hit
SSF47819, SSF47819, 1 hit
SSF52540, SSF52540, 1 hit
SSF53098, SSF53098, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00614, recQ_fam, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit
PS50967, HRDC, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O09053-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METTSLQRKF PEWMSMQSQR CATEEKACVQ KSVLEDNLPF LEFPGSIVYS
60 70 80 90 100
YEASDCSFLS EDISMRLSDG DVVGFDMEWP PIYKPGKRSR VAVIQLCVSE
110 120 130 140 150
SKCYLFHISS MSVFPQGLKM LLENKSIKKA GVGIEGDQWK LLRDFDVKLE
160 170 180 190 200
SFVELTDVAN EKLKCAETWS LNGLVKHVLG KQLLKDKSIR CSNWSNFPLT
210 220 230 240 250
EDQKLYAATD AYAGLIIYQK LGNLGDTAQV FALNKAEENL PLEMKKQLNS
260 270 280 290 300
ISEEMRDLAN RFPVTCRNLE TLQRVPVILK SISENLCSLR KVICGPTNTE
310 320 330 340 350
TRLKPGSSFN LLSSEDSAAA GEKEKQIGKH STFAKIKEEP WDPELDSLVK
360 370 380 390 400
QEEVDVFRNQ VKQEKGESEN EIEDNLLRED MERTCVIPSI SENELQDLEQ
410 420 430 440 450
QAKEEKYNDV SHQLSEHLSP NDDENDSSYI IESDEDLEME MLKSLENLNS
460 470 480 490 500
DVVEPTHSTW LEMGTNGRLP PEEEDGHGNE AIKEEQEEED HLLPEPNAKQ
510 520 530 540 550
INCLKTYFGH SSFKPVQWKV IHSVLEERRD NVVVMATGYG KSLCFQYPPV
560 570 580 590 600
YTGKIGIVIS PLISLMEDQV LQLELSNVPA CLLGSAQSKN ILGDVKLGKY
610 620 630 640 650
RVIYITPEFC SGNLDLLQQL DSSIGITLIA VDEAHCISEW GHDFRSSFRM
660 670 680 690 700
LGSLKTALPL VPVIALSATA SSSIREDIIS CLNLKDPQIT CTGFDRPNLY
710 720 730 740 750
LEVGRKTGNI LQDLKPFLVR KASSAWEFEG PTIIYCPSRK MTEQVTAELG
760 770 780 790 800
KLNLACRTYH AGMKISERKD VHHRFLRDEI QCVVATVAFG MGINKADIRK
810 820 830 840 850
VIHYGAPKEM ESYYQEIGRA GRDGLQSSCH LLWAPADFNT SRNLLIEIHD
860 870 880 890 900
EKFRLYKLKM MVKMEKYLHS SQCRRRIILS HFEDKCLQKA SLDIMGTEKC
910 920 930 940 950
CDNCRPRLNH CLTANNSEDA SQDFGPQAFQ LLSAVDILQE KFGIGIPILF
960 970 980 990 1000
LRGSNSQRLP DKYRGHRLFG AGKEQAESWW KTLSHHLIAE GFLVEVPKEN
1010 1020 1030 1040 1050
KYIKTCSLTK KGRKWLGEAS SQSPPSLLLQ ANEEMFPRKV LLPSSNPVSP
1060 1070 1080 1090 1100
ETTQHSSNQN PAGLTTKQSN LERTHSYKVP EKVSSGTNIP KKSAVMPSPG
1110 1120 1130 1140 1150
TSSSPLEPAI SAQELDARTG LYARLVEARQ KHANKMDVPP AILATNKVLL
1160 1170 1180 1190 1200
DMAKMRPTTV ENMKQIDGVS EGKAALLAPL LEVIKHFCQV TSVQTDLLSS
1210 1220 1230 1240 1250
AKPHKEQEKS QEMEKKDCSL PQSVAVTYTL FQEKKMPLHS IAENRLLPLT
1260 1270 1280 1290 1300
AAGMHLAQAV KAGYPLDMER AGLTPETWKI IMDVIRNPPI NSDMYKVKLI
1310 1320 1330 1340 1350
RMLVPENLDT YLIHMAIEIL QSGSDSRTQP PCDSSRKRRF PSSAESCESC
1360 1370 1380 1390 1400
KESKEAVTET KASSSESKRK LPEWFAKGNV PSADTGSSSS MAKTKKKGLF

S
Length:1,401
Mass (Da):157,204
Last modified:July 27, 2011 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i66DF2252B17C24C3
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GR54A0A1B0GR54_MOUSE
DNA helicase
Wrn
1,158Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti101S → N in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti101S → N in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti101S → N in AAC72359 (Ref. 2) Curated1
Sequence conflicti101S → N in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti228A → V in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti228A → V in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti228A → V in AAC72359 (Ref. 2) Curated1
Sequence conflicti228A → V in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti250S → L in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti250S → L in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti250S → L in AAC72359 (Ref. 2) Curated1
Sequence conflicti250S → L in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti452V → M in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti452V → M in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti452V → M in AAC72359 (Ref. 2) Curated1
Sequence conflicti452V → M in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti459T → K in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti459T → K in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti459T → K in AAC72359 (Ref. 2) Curated1
Sequence conflicti459T → K in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti468R → C in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti468R → C in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti468R → C in AAC72359 (Ref. 2) Curated1
Sequence conflicti468R → C in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti619Q → K in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti619Q → K in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti619Q → K in AAC72359 (Ref. 2) Curated1
Sequence conflicti619Q → K in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti800K → Q in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti800K → Q in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti844L → H in AAC72359 (Ref. 2) Curated1
Sequence conflicti955 – 988NSQRL…SHHLI → VSVSVIAPGTVSDSAFHCVA MALAFFRWLTSNPC in AAC72359 (Ref. 2) CuratedAdd BLAST34
Sequence conflicti1021S → L in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1021S → L in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1021S → L in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti1145T → A in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1145T → A in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1181L → V in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1181L → V in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1182E → G in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1182E → G in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1182E → G in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti1252A → V in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1252A → V in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1252A → V in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti1308L → I in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1308L → I in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1308L → I in AAF64490 (PubMed:10757812).Curated1
Sequence conflicti1356A → V in BAA20269 (PubMed:9143515).Curated1
Sequence conflicti1356A → V in BAA20270 (PubMed:9143515).Curated1
Sequence conflicti1356A → V in AAF64490 (PubMed:10757812).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D86527 mRNA Translation: BAA20270.1
D86526 mRNA Translation: BAA20269.1
AF091215 mRNA Translation: AAC78077.1
AF091216 Genomic DNA Translation: AAC72359.1
AF241636 mRNA Translation: AAF64490.1
AC153789 Genomic DNA No translation available.
AC115809 Genomic DNA No translation available.
BC050921 mRNA Translation: AAH50921.1
BC060700 mRNA Translation: AAH60700.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS22229.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T17452
T30247

NCBI Reference Sequences

More...
RefSeqi
NP_001116294.1, NM_001122822.1
NP_035851.3, NM_011721.4
XP_006509154.1, XM_006509091.3
XP_017168151.1, XM_017312662.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000033990; ENSMUSP00000033990; ENSMUSG00000031583
ENSMUST00000033991; ENSMUSP00000033991; ENSMUSG00000031583

Database of genes from NCBI RefSeq genomes

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GeneIDi
22427

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
mmu:22427

UCSC genome browser

More...
UCSCi
uc009ljw.1, mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86527 mRNA Translation: BAA20270.1
D86526 mRNA Translation: BAA20269.1
AF091215 mRNA Translation: AAC78077.1
AF091216 Genomic DNA Translation: AAC72359.1
AF241636 mRNA Translation: AAF64490.1
AC153789 Genomic DNA No translation available.
AC115809 Genomic DNA No translation available.
BC050921 mRNA Translation: AAH50921.1
BC060700 mRNA Translation: AAH60700.1
CCDSiCCDS22229.1
PIRiT17452
T30247
RefSeqiNP_001116294.1, NM_001122822.1
NP_035851.3, NM_011721.4
XP_006509154.1, XM_006509091.3
XP_017168151.1, XM_017312662.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E6LX-ray2.20A31-238[»]
2E6MX-ray2.00A31-238[»]
SMRiO09053
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi204584, 7 interactors
DIPiDIP-27642N
STRINGi10090.ENSMUSP00000033990

PTM databases

iPTMnetiO09053
PhosphoSitePlusiO09053

Proteomic databases

EPDiO09053
MaxQBiO09053
PaxDbiO09053
PeptideAtlasiO09053
PRIDEiO09053

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
10619, 286 antibodies

Genome annotation databases

EnsembliENSMUST00000033990; ENSMUSP00000033990; ENSMUSG00000031583
ENSMUST00000033991; ENSMUSP00000033991; ENSMUSG00000031583
GeneIDi22427
KEGGimmu:22427
UCSCiuc009ljw.1, mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
7486
MGIiMGI:109635, Wrn

Phylogenomic databases

eggNOGiKOG0351, Eukaryota
KOG4373, Eukaryota
GeneTreeiENSGT00940000159168
HOGENOMiCLU_001103_11_1_1
InParanoidiO09053
KOiK10900
OrthoDBi445763at2759
TreeFamiTF312852

Enzyme and pathway databases

ReactomeiR-MMU-174414, Processive synthesis on the C-strand of the telomere
R-MMU-174437, Removal of the Flap Intermediate from the C-strand
R-MMU-5685938, HDR through Single Strand Annealing (SSA)
R-MMU-5685942, HDR through Homologous Recombination (HRR)
R-MMU-5693554, Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA)
R-MMU-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579, Homologous DNA Pairing and Strand Exchange
R-MMU-5693607, Processing of DNA double-strand break ends
R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation
R-MMU-69473, G2/M DNA damage checkpoint

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
22427, 3 hits in 20 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Wrn, mouse
EvolutionaryTraceiO09053

Protein Ontology

More...
PROi
PR:O09053
RNActiO09053, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000031583, Expressed in secondary oocyte and 266 other tissues
ExpressionAtlasiO09053, baseline and differential
GenevisibleiO09053, MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR002562, 3'-5'_exonuclease_dom
IPR011545, DEAD/DEAH_box_helicase_dom
IPR004589, DNA_helicase_ATP-dep_RecQ
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR029491, Helicase_HTH
IPR010997, HRDC-like_sf
IPR002121, HRDC_dom
IPR027417, P-loop_NTPase
IPR032284, RecQ_Zn-bd
IPR012337, RNaseH-like_sf
IPR036397, RNaseH_sf
IPR018982, RQC_domain
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf
PfamiView protein in Pfam
PF00270, DEAD, 1 hit
PF01612, DNA_pol_A_exo1, 1 hit
PF00271, Helicase_C, 1 hit
PF00570, HRDC, 1 hit
PF14493, HTH_40, 1 hit
PF16124, RecQ_Zn_bind, 1 hit
PF09382, RQC, 1 hit
SMARTiView protein in SMART
SM00474, 35EXOc, 1 hit
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit
SM00341, HRDC, 1 hit
SM00956, RQC, 1 hit
SUPFAMiSSF46785, SSF46785, 1 hit
SSF47819, SSF47819, 1 hit
SSF52540, SSF52540, 1 hit
SSF53098, SSF53098, 1 hit
TIGRFAMsiTIGR00614, recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit
PS50967, HRDC, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiWRN_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O09053
Secondary accession number(s): O09050
, Q80YP9, Q9JKD4, Q9Z241, Q9Z242
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: August 12, 2020
This is version 185 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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