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Protein

Regenerating islet-derived protein 3-gamma

Gene

Reg3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.7 Publications

GO - Molecular functioni

  • oligosaccharide binding Source: UniProtKB
  • peptidoglycan binding Source: UniProtKB
  • transmembrane signaling receptor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial
Biological processAcute phase, Inflammatory response
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-6803157 Antimicrobial peptides

Protein family/group databases

MEROPSiI63.002
TCDBi1.C.111.1.1 the regiii (regiii) family

Names & Taxonomyi

Protein namesi
Recommended name:
Regenerating islet-derived protein 3-gamma
Short name:
REG-3-gamma
Alternative name(s):
Pancreatitis-associated protein 3
Regenerating islet-derived protein III-gamma
Short name:
Reg III-gamma
Cleaved into the following 2 chains:
Gene namesi
Name:Reg3g
Synonyms:Pap3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:109406 Reg3g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at normal Mendelian ratios. They appear healthy and show no signs of enteropathy, but exhibit a marked increase in the number of mucosa-associated bacteria, predominantly Gram-positive, relative to cohoused wild-type littermates in distal small intestine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000001743527 – 174Regenerating islet-derived protein 3-gamma 16.5 kDa formAdd BLAST148
PropeptideiPRO_000042275327 – 371 PublicationAdd BLAST11
ChainiPRO_000042275438 – 174Regenerating islet-derived protein 3-gamma 15 kDa formAdd BLAST137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 51PROSITE-ProRule annotation
Disulfide bondi68 ↔ 170PROSITE-ProRule annotation
Disulfide bondi145 ↔ 162PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO09049
PeptideAtlasiO09049
PRIDEiO09049

Expressioni

Tissue specificityi

Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin injury increases its epidermal expression. Also expressed in the pancreas.7 Publications

Developmental stagei

In mid-small intestine, very low levels at birth. Expression levels rise dramatically during the weaning period (P17-P22) and remain high into adulthood in conventionally raised but not germfree animals.1 Publication

Inductioni

Up-regulated in Paneth cells by intestinal microbiota (at protein level). MyD88-mediated signals are essential for its induction in intestinal epithelial cells. Induction in the lung is dependent on IL6ST-induced STAT3 signaling. IL17A induces its expression in primary keratinocytes and skin wounds.4 Publications

Gene expression databases

BgeeiENSMUSG00000030017 Expressed in 66 organ(s), highest expression level in ileum
CleanExiMM_REG3G
ExpressionAtlasiO09049 baseline and differential
GenevisibleiO09049 MM

Interactioni

Protein-protein interaction databases

IntActiO09049, 2 interactors
MINTiO09049
STRINGi10090.ENSMUSP00000032089

Structurei

3D structure databases

ProteinModelPortaliO09049
SMRiO09049
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 171C-type lectinPROSITE-ProRule annotationAdd BLAST125

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi114 – 116EPNBy similarity3

Domaini

The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JQFS Eukaryota
ENOG4111DBG LUCA
GeneTreeiENSGT00700000104249
HOGENOMiHOG000010281
HOVERGENiHBG004151
InParanoidiO09049
OMAiQKRPSGK
OrthoDBiEOG091G0MDY
PhylomeDBiO09049

Family and domain databases

Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O09049-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLPRITITIM SWMLLSCLML LSQVQGEVAK KDAPSSRSSC PKGSRAYGSY
60 70 80 90 100
CYALFSVSKN WYDADMACQK RPSGHLVSVL SGAEASFLSS MIKSSGNSGQ
110 120 130 140 150
YVWIGLHDPT LGYEPNRGGW EWSNADVMNY INWETNPSSS SGNHCGTLSR
160 170
ASGFLKWREN YCNLELPYVC KFKA
Length:174
Mass (Da):19,307
Last modified:July 1, 1997 - v1
Checksum:i5575E9E56A4D8CEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63361 mRNA Translation: BAA18930.1
D63362 Genomic DNA Translation: BAA18931.1
BC061139 mRNA Translation: AAH61139.1
CCDSiCCDS20258.1
RefSeqiNP_035390.1, NM_011260.2
UniGeneiMm.252385

Genome annotation databases

EnsembliENSMUST00000032089; ENSMUSP00000032089; ENSMUSG00000030017
GeneIDi19695
KEGGimmu:19695
UCSCiuc009ckb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63361 mRNA Translation: BAA18930.1
D63362 Genomic DNA Translation: BAA18931.1
BC061139 mRNA Translation: AAH61139.1
CCDSiCCDS20258.1
RefSeqiNP_035390.1, NM_011260.2
UniGeneiMm.252385

3D structure databases

ProteinModelPortaliO09049
SMRiO09049
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO09049, 2 interactors
MINTiO09049
STRINGi10090.ENSMUSP00000032089

Protein family/group databases

MEROPSiI63.002
TCDBi1.C.111.1.1 the regiii (regiii) family

Proteomic databases

PaxDbiO09049
PeptideAtlasiO09049
PRIDEiO09049

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032089; ENSMUSP00000032089; ENSMUSG00000030017
GeneIDi19695
KEGGimmu:19695
UCSCiuc009ckb.1 mouse

Organism-specific databases

CTDi130120
MGIiMGI:109406 Reg3g

Phylogenomic databases

eggNOGiENOG410JQFS Eukaryota
ENOG4111DBG LUCA
GeneTreeiENSGT00700000104249
HOGENOMiHOG000010281
HOVERGENiHBG004151
InParanoidiO09049
OMAiQKRPSGK
OrthoDBiEOG091G0MDY
PhylomeDBiO09049

Enzyme and pathway databases

ReactomeiR-MMU-6803157 Antimicrobial peptides

Miscellaneous databases

ChiTaRSiReg3g mouse
PROiPR:O09049
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030017 Expressed in 66 organ(s), highest expression level in ileum
CleanExiMM_REG3G
ExpressionAtlasiO09049 baseline and differential
GenevisibleiO09049 MM

Family and domain databases

Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiREG3G_MOUSE
AccessioniPrimary (citable) accession number: O09049
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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