UniProtKB - O09044 (SNP23_MOUSE)
Synaptosomal-associated protein 23
Snap23
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 185 – 186 | (Microbial infection) Cleavage; by C.botulinum neurotoxin type E (BoNT/E)1 Publication | 2 | |
Sitei | 202 – 203 | (Microbial infection) Cleavage; by C.botulinum neurotoxin type A (BoNT/A, botA)1 Publication | 2 |
GO - Molecular functioni
- SNAP receptor activity Source: MGI
- syntaxin binding Source: MGI
GO - Biological processi
- exocytic insertion of neurotransmitter receptor to postsynaptic membrane Source: MGI
- exocytosis Source: MGI
- histamine secretion by mast cell Source: MGI
- membrane fusion Source: MGI
- protein-containing complex assembly Source: MGI
- protein transport Source: MGI
- regulation of exocytosis Source: MGI
- synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
- synaptic vesicle priming Source: GO_Central
- vesicle fusion Source: GO_Central
- vesicle-mediated transport Source: MGI
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-199992, trans-Golgi Network Vesicle Budding R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptosomal-associated protein 23Short name: SNAP-23 Alternative name(s): Syndet Vesicle-membrane fusion protein SNAP-23 |
Gene namesi | Name:Snap23 Synonyms:Sndt |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109356, Snap23 |
Subcellular locationi
Plasma membrane
- Cell membrane ; Peripheral membrane protein
- Cell membrane By similarity; Lipid-anchor By similarity
Other locations
Note: Mainly localized to the plasma membrane.
Lysosome
- azurophil granule Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- adherens junction Source: MGI
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- neuron projection Source: UniProtKB-SubCell
- presynapse Source: GOC
- SNARE complex Source: MGI
- specific granule Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 119 | P → A: Inhibits interaction with ZDHHC13 and ZDHHC17. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213599 | 1 – 210 | Synaptosomal-associated protein 23Add BLAST | 210 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 5 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Lipidationi | 79 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 80 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 83 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 85 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 87 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 160 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | O09044 |
jPOSTi | O09044 |
MaxQBi | O09044 |
PaxDbi | O09044 |
PRIDEi | O09044 |
PTM databases
iPTMneti | O09044 |
PhosphoSitePlusi | O09044 |
SwissPalmi | O09044 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000027287, Expressed in blood and 297 other tissues |
ExpressionAtlasi | O09044, baseline and differential |
Genevisiblei | O09044, MM |
Interactioni
Subunit structurei
Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3 (By similarity).
Found in a complex with VAMP8 and STX1A (By similarity).
Found in a complex with VAMP8 and STX4 in pancreas (PubMed:15363411).
Interacts simultaneously with SNAPIN and SYN4 (By similarity).
Interacts with STX1A (PubMed:9507000).
Interacts with STX12 (PubMed:9507000).
Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (PubMed:26198635).
Interacts with ZDHHC17 (via ANK repeats) (PubMed:26198635).
By similarity3 PublicationsBinary interactionsi
Hide detailsO09044
With | #Exp. | IntAct |
---|---|---|
Stx4 [P70452] | 2 | EBI-1812522,EBI-645716 |
Vamp2 [P63044] | 3 | EBI-1812522,EBI-521920 |
Stx4 [Q08850] from Rattus norvegicus. | 4 | EBI-1812522,EBI-918243 |
GO - Molecular functioni
- syntaxin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203367, 15 interactors |
CORUMi | O09044 |
DIPi | DIP-41401N |
IntActi | O09044, 10 interactors |
MINTi | O09044 |
STRINGi | 10090.ENSMUSP00000112138 |
Miscellaneous databases
RNActi | O09044, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 76 | t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 145 – 207 | t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 23 – 76 | By similarityAdd BLAST | 54 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG3065, Eukaryota |
GeneTreei | ENSGT00950000182843 |
HOGENOMi | CLU_096939_0_0_1 |
InParanoidi | O09044 |
OMAi | QINRTHV |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O09044 |
Family and domain databases
InterProi | View protein in InterPro IPR000928, SNAP-25_dom IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF00835, SNAP-25, 1 hit |
SMARTi | View protein in SMART SM00397, t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192, T_SNARE, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDNLSPEEVQ LRAHQVTDES LESTRRILGL AIESQDAGIK TITMLDEQGE
60 70 80 90 100
QLNRIEEGMD QINKDMREAE KTLTELNKCC GLCICPCNRT KNFESGKNYK
110 120 130 140 150
ATWGDGGDNS PSNVVSKQPS RITNGQPQQT TGAASGGYIK RITNDAREDE
160 170 180 190 200
MEENLTQVGS ILGNLKNMAL DMGNEIDAQN QQIQKITEKA DTNKNRIDIA
210
NTRAKKLIDS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ9D3L3 | Q9D3L3_MOUSE | Synaptosomal-associated protein | Snap23 | 221 | Annotation score: | ||
B0R030 | B0R030_MOUSE | Synaptosomal-associated protein | Snap23 | 161 | Annotation score: | ||
B0R029 | B0R029_MOUSE | Synaptosomal-associated protein 23 | Snap23 | 85 | Annotation score: | ||
E9Q8A1 | E9Q8A1_MOUSE | Synaptosomal-associated protein 23 | Snap23 | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | P → S in AAB62932 (Ref. 3) Curated | 1 | |
Sequence conflicti | 204 | A → P in AAB62932 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000822 mRNA Translation: BAA20345.1 U73143 mRNA Translation: AAB53597.1 AF007169 mRNA Translation: AAB62932.1 AF213257 , AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA Translation: AAF23503.1 AK019162 mRNA Translation: BAB31577.1 BC070456 mRNA Translation: AAH70456.1 |
CCDSi | CCDS16622.1 |
PIRi | JC5512 |
RefSeqi | NP_001171263.1, NM_001177792.1 NP_001171264.1, NM_001177793.1 NP_033248.1, NM_009222.3 XP_006499120.1, XM_006499057.3 |
Genome annotation databases
Ensembli | ENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287 ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287 |
GeneIDi | 20619 |
KEGGi | mmu:20619 |
UCSCi | uc008lwg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000822 mRNA Translation: BAA20345.1 U73143 mRNA Translation: AAB53597.1 AF007169 mRNA Translation: AAB62932.1 AF213257 , AF213251, AF213252, AF213253, AF213254, AF213255, AF213256 Genomic DNA Translation: AAF23503.1 AK019162 mRNA Translation: BAB31577.1 BC070456 mRNA Translation: AAH70456.1 |
CCDSi | CCDS16622.1 |
PIRi | JC5512 |
RefSeqi | NP_001171263.1, NM_001177792.1 NP_001171264.1, NM_001177793.1 NP_033248.1, NM_009222.3 XP_006499120.1, XM_006499057.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 203367, 15 interactors |
CORUMi | O09044 |
DIPi | DIP-41401N |
IntActi | O09044, 10 interactors |
MINTi | O09044 |
STRINGi | 10090.ENSMUSP00000112138 |
PTM databases
iPTMneti | O09044 |
PhosphoSitePlusi | O09044 |
SwissPalmi | O09044 |
Proteomic databases
EPDi | O09044 |
jPOSTi | O09044 |
MaxQBi | O09044 |
PaxDbi | O09044 |
PRIDEi | O09044 |
Protocols and materials databases
Antibodypediai | 712, 412 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028743; ENSMUSP00000028743; ENSMUSG00000027287 ENSMUST00000110711; ENSMUSP00000106339; ENSMUSG00000027287 |
GeneIDi | 20619 |
KEGGi | mmu:20619 |
UCSCi | uc008lwg.1, mouse |
Organism-specific databases
CTDi | 8773 |
MGIi | MGI:109356, Snap23 |
Phylogenomic databases
eggNOGi | KOG3065, Eukaryota |
GeneTreei | ENSGT00950000182843 |
HOGENOMi | CLU_096939_0_0_1 |
InParanoidi | O09044 |
OMAi | QINRTHV |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O09044 |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-199992, trans-Golgi Network Vesicle Budding R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 20619, 7 hits in 18 CRISPR screens |
ChiTaRSi | Snap23, mouse |
PROi | PR:O09044 |
RNActi | O09044, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027287, Expressed in blood and 297 other tissues |
ExpressionAtlasi | O09044, baseline and differential |
Genevisiblei | O09044, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000928, SNAP-25_dom IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF00835, SNAP-25, 1 hit |
SMARTi | View protein in SMART SM00397, t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192, T_SNARE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SNP23_MOUSE | |
Accessioni | O09044Primary (citable) accession number: O09044 Secondary accession number(s): O35620 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families