UniProtKB - O09043 (NAPSA_MOUSE)
Protein
Napsin-A
Gene
Napsa
Organism
Mus musculus (Mouse)
Status
Functioni
May be involved in processing of pneumocyte surfactant precursors.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | PROSITE-ProRule annotation | 1 | |
Active sitei | 278 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
- endopeptidase activity Source: UniProtKB
- peptidase activity Source: MGI
GO - Biological processi
- membrane protein proteolysis Source: UniProtKB
- surfactant homeostasis Source: MGI
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Enzyme and pathway databases
BRENDAi | 3.4.23.B1, 3474 |
Reactomei | R-MMU-5683826, Surfactant metabolism |
Protein family/group databases
MEROPSi | A01.046 |
Names & Taxonomyi
Protein namesi | Recommended name: Napsin-A (EC:3.4.23.-)Alternative name(s): KDAP-1 Kidney-derived aspartic protease-like protein Short name: KAP |
Gene namesi | Name:Napsa Synonyms:Kdap, Nap |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109365, Napsa |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular space Source: MGI
Lysosome
- lysosome Source: UniProtKB
Other locations
- alveolar lamellar body Source: UniProtKB
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
PropeptideiPRO_0000025998 | 17 – ? | Activation peptideSequence analysis | ||
ChainiPRO_0000025999 | ? – 419 | Napsin-A |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 85 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 104 ↔ 111 | By similarity | ||
Glycosylationi | 128 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 149 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 269 ↔ 273 | By similarity | ||
Disulfide bondi | 312 ↔ 349 | By similarity | ||
Glycosylationi | 331 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
EPDi | O09043 |
jPOSTi | O09043 |
PaxDbi | O09043 |
PeptideAtlasi | O09043 |
PRIDEi | O09043 |
PTM databases
GlyGeni | O09043, 4 sites |
iPTMneti | O09043 |
PhosphoSitePlusi | O09043 |
Expressioni
Tissue specificityi
Expressed at the highest levels in the kidney, at a moderate level in the lung, and at low levels in the spleen and adipose tissue.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000002204, Expressed in metanephros and 139 other tissues |
Genevisiblei | O09043, MM |
Interactioni
Protein-protein interaction databases
IntActi | O09043, 3 interactors |
MINTi | O09043 |
STRINGi | 10090.ENSMUSP00000002274 |
Miscellaneous databases
RNActi | O09043, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 394 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 322 |
Sequence similaritiesi
Belongs to the peptidase A1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000160179 |
HOGENOMi | CLU_013253_3_3_1 |
InParanoidi | O09043 |
OMAi | MNVQYYG |
OrthoDBi | 1619495at2759 |
PhylomeDBi | O09043 |
TreeFami | TF314990 |
Family and domain databases
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR13683, PTHR13683, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O09043-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPLLLLLLC LLLGNLEPEE AKLIRVPLQR IHLGHRILNP LNGWEQLAEL
60 70 80 90 100
SRTSTSGGNP SFVPLSKFMN TQYFGTIGLG TPPQNFTVVF DTGSSNLWVP
110 120 130 140 150
STRCHFFSLA CWFHHRFNPK ASSSFRPNGT KFAIQYGTGR LSGILSQDNL
160 170 180 190 200
TIGGIHDAFV TFGEALWEPS LIFALAHFDG ILGLGFPTLA VGGVQPPLDA
210 220 230 240 250
MVEQGLLEKP VFSFYLNRDS EGSDGGELVL GGSDPAHYVP PLTFIPVTIP
260 270 280 290 300
AYWQVHMESV KVGTGLSLCA QGCSAILDTG TSLITGPSEE IRALNKAIGG
310 320 330 340 350
YPFLNGQYFI QCSKTPTLPP VSFHLGGVWF NLTGQDYVIK ILQSDVGLCL
360 370 380 390 400
LGFQALDIPK PAGPLWILGD VFLGPYVAVF DRGDKNVGPR VGLARAQSRS
410
TDRAERRTTQ AQFFKRRPG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIE6 | A0A140LIE6_MOUSE | Napsin-A | Napsa | 64 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88899 mRNA Translation: BAA19004.1 AJ250718, AJ250719, AJ250720 Genomic DNA Translation: CAB82907.1 BC014813 mRNA Translation: AAH14813.1 |
CCDSi | CCDS21212.1 |
RefSeqi | NP_032463.1, NM_008437.1 |
Genome annotation databases
Ensembli | ENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204 |
GeneIDi | 16541 |
KEGGi | mmu:16541 |
UCSCi | uc009gqe.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88899 mRNA Translation: BAA19004.1 AJ250718, AJ250719, AJ250720 Genomic DNA Translation: CAB82907.1 BC014813 mRNA Translation: AAH14813.1 |
CCDSi | CCDS21212.1 |
RefSeqi | NP_032463.1, NM_008437.1 |
3D structure databases
SMRi | O09043 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O09043, 3 interactors |
MINTi | O09043 |
STRINGi | 10090.ENSMUSP00000002274 |
Protein family/group databases
MEROPSi | A01.046 |
PTM databases
GlyGeni | O09043, 4 sites |
iPTMneti | O09043 |
PhosphoSitePlusi | O09043 |
Proteomic databases
EPDi | O09043 |
jPOSTi | O09043 |
PaxDbi | O09043 |
PeptideAtlasi | O09043 |
PRIDEi | O09043 |
Protocols and materials databases
Antibodypediai | 3755, 768 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000002274; ENSMUSP00000002274; ENSMUSG00000002204 |
GeneIDi | 16541 |
KEGGi | mmu:16541 |
UCSCi | uc009gqe.1, mouse |
Organism-specific databases
CTDi | 9476 |
MGIi | MGI:109365, Napsa |
Phylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000160179 |
HOGENOMi | CLU_013253_3_3_1 |
InParanoidi | O09043 |
OMAi | MNVQYYG |
OrthoDBi | 1619495at2759 |
PhylomeDBi | O09043 |
TreeFami | TF314990 |
Enzyme and pathway databases
BRENDAi | 3.4.23.B1, 3474 |
Reactomei | R-MMU-5683826, Surfactant metabolism |
Miscellaneous databases
BioGRID-ORCSi | 16541, 1 hit in 17 CRISPR screens |
ChiTaRSi | Napsa, mouse |
PROi | PR:O09043 |
RNActi | O09043, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002204, Expressed in metanephros and 139 other tissues |
Genevisiblei | O09043, MM |
Family and domain databases
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR13683, PTHR13683, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NAPSA_MOUSE | |
Accessioni | O09043Primary (citable) accession number: O09043 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries