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Protein

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene

Lfng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (PubMed:28089369). Decreases the binding of JAG1 to NOTCH2 but not that of DLL1 (By similarity). Essential mediator of somite segmentation and patterning. During somite boundary formation, it restricts Notch activity in the presomitic mesoderm to a boundary-forming territory in the posterior half of the prospective somite. In this region, Notch function activates a set of genes that are involved in boundary formation and in anterior-posterior somite identity (PubMed:10330372). Ectopically expressed in the thymus, Lfgn inhibits Notch signaling which results in inhibition of T-cell commitment and promotes B-cell development in lymphoid progenitors (PubMed:11520458). May play a role in boundary formation of the enamel knot (PubMed:12167404).By similarity4 Publications

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei128SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Metal bindingi201ManganeseBy similarity1
Active sitei289By similarity1
Metal bindingi313ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.222 3474

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Lfng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1095413 Lfng

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 378LumenalSequence analysisAdd BLAST349

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191771 – 378Beta-1,3-N-acetylglucosaminyltransferase lunatic fringeAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi167 ↔ 178By similarity
Disulfide bondi196 ↔ 259By similarity
Disulfide bondi363 ↔ 372By similarity

Post-translational modificationi

A soluble form may be derived from the membrane form by proteolytic processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei85 – 86Cleavage; by furin-like proteaseSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO09010
PaxDbiO09010
PRIDEiO09010

PTM databases

iPTMnetiO09010
PhosphoSitePlusiO09010

Expressioni

Tissue specificityi

Detected at 12.5 dpc in all tissues examined with the highest level observed in adult brain and spleen. Detected in the dental epithelium.

Developmental stagei

Developmental protein. During segmentation it shows a cyclic transcription pattern which is under the control of Notch. Expressed in the caudal region of the presomitic mesoderm with each cycle corresponding to the formation time of one somite. In the dental epithelium it is detected at stage E13.5. The pattern of expression corresponds exactly to the formation of the enamel knot between late bud and early cap stages.4 Publications

Gene expression databases

BgeeiENSMUSG00000029570 Expressed in 251 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiO09010 baseline and differential
GenevisibleiO09010 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031555

Structurei

3D structure databases

ProteinModelPortaliO09010
SMRiO09010
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiO09010
KOiK05948
OMAiPHTQDVY
OrthoDBiEOG091G08XO
PhylomeDBiO09010
TreeFamiTF324207

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit

Sequencei

Sequence statusi: Complete.

O09010-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLQRCGRRLL LALVGALLAC LLVLTADPPP TPMPAERGRR ALRSLAGSSG
60 70 80 90 100
GAPASGSRAA VDPGVLTREV HSLSEYFSLL TRARRDADPP PGVASRQGDG
110 120 130 140 150
HPRPPAEVLS PRDVFIAVKT TRKFHRARLD LLFETWISRH KEMTFIFTDG
160 170 180 190 200
EDEALAKLTG NVVLTNCSSA HSRQALSCKM AVEYDRFIES GKKWFCHVDD
210 220 230 240 250
DNYVNLRALL RLLASYPHTQ DVYIGKPSLD RPIQATERIS EHKVRPVHFW
260 270 280 290 300
FATGGAGFCI SRGLALKMGP WASGGHFMST AERIRLPDDC TIGYIVEALL
310 320 330 340 350
GVPLIRSGLF HSHLENLQQV PTTELHEQVT LSYGMFENKR NAVHIKGPFS
360 370
VEADPSRFRS VHCHLYPDTP WCPRSAIF
Length:378
Mass (Da):41,952
Last modified:July 1, 1997 - v1
Checksum:iFD0A02597BF9AFED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti343V → M in AK004642 (PubMed:16141072).Curated1
Sequence conflicti361V → I in AK004642 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94351 mRNA Translation: AAC53262.1
AF015768 mRNA Translation: AAB71668.1
AY124581 Genomic DNA Translation: AAM93541.1
AK004642 mRNA No translation available.
AK153283 mRNA Translation: BAE31866.1
CCDSiCCDS19821.1
RefSeqiNP_032520.1, NM_008494.3
UniGeneiMm.12834

Genome annotation databases

EnsembliENSMUST00000031555; ENSMUSP00000031555; ENSMUSG00000029570
GeneIDi16848
KEGGimmu:16848
UCSCiuc009ahu.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

lunatic fringe gene homolog (lfng)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94351 mRNA Translation: AAC53262.1
AF015768 mRNA Translation: AAB71668.1
AY124581 Genomic DNA Translation: AAM93541.1
AK004642 mRNA No translation available.
AK153283 mRNA Translation: BAE31866.1
CCDSiCCDS19821.1
RefSeqiNP_032520.1, NM_008494.3
UniGeneiMm.12834

3D structure databases

ProteinModelPortaliO09010
SMRiO09010
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031555

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

PTM databases

iPTMnetiO09010
PhosphoSitePlusiO09010

Proteomic databases

MaxQBiO09010
PaxDbiO09010
PRIDEiO09010

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031555; ENSMUSP00000031555; ENSMUSG00000029570
GeneIDi16848
KEGGimmu:16848
UCSCiuc009ahu.1 mouse

Organism-specific databases

CTDi3955
MGIiMGI:1095413 Lfng

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiO09010
KOiK05948
OMAiPHTQDVY
OrthoDBiEOG091G08XO
PhylomeDBiO09010
TreeFamiTF324207

Enzyme and pathway databases

BRENDAi2.4.1.222 3474

Miscellaneous databases

PROiPR:O09010
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029570 Expressed in 251 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiO09010 baseline and differential
GenevisibleiO09010 MM

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLFNG_MOUSE
AccessioniPrimary (citable) accession number: O09010
Secondary accession number(s): Q3U659, Q8K3F1, Q9DC10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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