UniProtKB - O09009 (RFNG_MOUSE)
Protein
Beta-1,3-N-acetylglucosaminyltransferase radical fringe
Gene
Rfng
Organism
Mus musculus (Mouse)
Status
Functioni
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1 (PubMed:28089369). May be involved in limb formation and in neurogenesis (By similarity).By similarity1 Publication
Catalytic activityi
- Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor. EC:2.4.1.222
Cofactori
Mn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | SubstrateBy similarity | 1 | |
Binding sitei | 148 | SubstrateBy similarity | 1 | |
Metal bindingi | 149 | ManganeseBy similarity | 1 | |
Active sitei | 238 | By similarity | 1 | |
Metal bindingi | 262 | ManganeseBy similarity | 1 |
GO - Molecular functioni
- acetylglucosaminyltransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity Source: GO_Central
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- negative regulation of Notch signaling pathway Source: MGI
- nervous system development Source: UniProtKB-KW
- pattern specification process Source: InterPro
- positive regulation of Notch signaling pathway Source: MGI
- positive regulation of protein binding Source: MGI
- protein O-linked fucosylation Source: UniProtKB
- regulation of Notch signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Glycosyltransferase, Transferase |
Biological process | Differentiation, Neurogenesis |
Ligand | Manganese, Metal-binding |
Protein family/group databases
CAZyi | GT31 Glycosyltransferase Family 31 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1,3-N-acetylglucosaminyltransferase radical fringe (EC:2.4.1.222)Alternative name(s): O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
Gene namesi | Name:Rfng |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894275 Rfng |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- integral component of Golgi membrane Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 30 – 332 | LumenalSequence analysisAdd BLAST | 303 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219186 | 1 – 332 | Beta-1,3-N-acetylglucosaminyltransferase radical fringeAdd BLAST | 332 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 115 ↔ 126 | By similarity | ||
Disulfide bondi | 144 ↔ 208 | By similarity | ||
Disulfide bondi | 312 ↔ 321 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | O09009 |
PaxDbi | O09009 |
PRIDEi | O09009 |
PTM databases
iPTMneti | O09009 |
PhosphoSitePlusi | O09009 |
Expressioni
Tissue specificityi
Detected in all the examined tissues (12.5 dpc). High expression found in adult brain.
Gene expression databases
Bgeei | ENSMUSG00000025158 Expressed in 288 organ(s), highest expression level in CA1 field of hippocampus |
Genevisiblei | O09009 MM |
Structurei
3D structure databases
ProteinModelPortali | O09009 |
SMRi | O09009 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 31 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410II9W Eukaryota ENOG410XS8Y LUCA |
GeneTreei | ENSGT00940000160801 |
HOGENOMi | HOG000046678 |
HOVERGENi | HBG007986 |
InParanoidi | O09009 |
KOi | K05948 |
OMAi | TTRKNHG |
OrthoDBi | 826272at2759 |
PhylomeDBi | O09009 |
TreeFami | TF324207 |
Family and domain databases
InterProi | View protein in InterPro IPR017374 Fringe IPR003378 Fringe-like |
Pfami | View protein in Pfam PF02434 Fringe, 1 hit |
PIRSFi | PIRSF038073 B-acetylgalactosaminyltfrase, 1 hit |
i Sequence
Sequence statusi: Complete.
O09009-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRARRVLCR ACLALAAVLA VLLLLPLPLP LPLPRAPAPD PDRVPTRSLT
60 70 80 90 100
LEGDRLQPDD VFIAVKTTRK NHGPRLRLLL RTWISRAPRQ TFIFTDGDDP
110 120 130 140 150
ELQMLAGGRM INTNCSAVRT RQALCCKMSV EYDKFLESGR KWFCHVDDDN
160 170 180 190 200
YVNPKSLLHL LSTFSSNQDI YLGRPSLDHP IEATERVQGG GTSNTVKFWF
210 220 230 240 250
ATGGAGFCLS RGLALKMSPW ASLGSFMSTA ERVRLPDDCT VGYIVEGLLG
260 270 280 290 300
ARLLHSPLFH SHLENLQRLP SGAILQQVTL SYGGPENPHN VVNVAGSFNI
310 320 330
QQDPTRFQSV HCLLYPDTHW CPMKNRVEGA FQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U94350 mRNA Translation: AAC53261.1 AF015770 mRNA Translation: AAB71670.1 AL663090 Genomic DNA No translation available. BC066023 mRNA Translation: AAH66023.1 |
CCDSi | CCDS25757.1 |
RefSeqi | NP_033079.1, NM_009053.2 |
UniGenei | Mm.871 |
Genome annotation databases
Ensembli | ENSMUST00000026156; ENSMUSP00000026156; ENSMUSG00000025158 |
GeneIDi | 19719 |
KEGGi | mmu:19719 |
UCSCi | uc007mum.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U94350 mRNA Translation: AAC53261.1 AF015770 mRNA Translation: AAB71670.1 AL663090 Genomic DNA No translation available. BC066023 mRNA Translation: AAH66023.1 |
CCDSi | CCDS25757.1 |
RefSeqi | NP_033079.1, NM_009053.2 |
UniGenei | Mm.871 |
3D structure databases
ProteinModelPortali | O09009 |
SMRi | O09009 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000026156 |
Protein family/group databases
CAZyi | GT31 Glycosyltransferase Family 31 |
PTM databases
iPTMneti | O09009 |
PhosphoSitePlusi | O09009 |
Proteomic databases
EPDi | O09009 |
PaxDbi | O09009 |
PRIDEi | O09009 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000026156; ENSMUSP00000026156; ENSMUSG00000025158 |
GeneIDi | 19719 |
KEGGi | mmu:19719 |
UCSCi | uc007mum.1 mouse |
Organism-specific databases
CTDi | 5986 |
MGIi | MGI:894275 Rfng |
Phylogenomic databases
eggNOGi | ENOG410II9W Eukaryota ENOG410XS8Y LUCA |
GeneTreei | ENSGT00940000160801 |
HOGENOMi | HOG000046678 |
HOVERGENi | HBG007986 |
InParanoidi | O09009 |
KOi | K05948 |
OMAi | TTRKNHG |
OrthoDBi | 826272at2759 |
PhylomeDBi | O09009 |
TreeFami | TF324207 |
Miscellaneous databases
PROi | PR:O09009 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025158 Expressed in 288 organ(s), highest expression level in CA1 field of hippocampus |
Genevisiblei | O09009 MM |
Family and domain databases
InterProi | View protein in InterPro IPR017374 Fringe IPR003378 Fringe-like |
Pfami | View protein in Pfam PF02434 Fringe, 1 hit |
PIRSFi | PIRSF038073 B-acetylgalactosaminyltfrase, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | RFNG_MOUSE | |
Accessioni | O09009Primary (citable) accession number: O09009 Secondary accession number(s): B1ATU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2003 |
Last sequence update: | July 1, 1997 | |
Last modified: | January 16, 2019 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot