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Protein

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Gene

Mfng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1.1 Publication

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70Substrate1
Binding sitei143Substrate1
Metal bindingi144Manganese1
Active sitei2321 Publication1
Metal bindingi256Manganese1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.222 3474

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase manic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:Mfng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1095404 Mfng

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini28 – 321LumenalSequence analysisAdd BLAST294

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191831 – 321Beta-1,3-N-acetylglucosaminyltransferase manic fringeAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi110 ↔ 1211 Publication
Disulfide bondi139 ↔ 2021 Publication
Glycosylationi185N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi306 ↔ 3151 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO09008
PRIDEiO09008

PTM databases

PhosphoSitePlusiO09008

Expressioni

Gene expression databases

BgeeiENSMUSG00000018169 Expressed in 222 organ(s), highest expression level in cerebral cortex
ExpressionAtlasiO09008 baseline and differential
GenevisibleiO09008 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018313

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO09008
SMRiO09008
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO09008

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiO09008
KOiK05948
OMAiNRPIRAS
OrthoDBiEOG091G08XO
PhylomeDBiO09008
TreeFamiTF324207

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

O09008-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHCRLFRGMA GALFTLLCVG LLSLRYHSSL SQRMIQGALR LNQRNPGPLE
60 70 80 90 100
LQLGDIFIAV KTTWAFHRSR LDLLLDTWVS RIRQQTFIFT DSPDERLQER
110 120 130 140 150
LGPHLVVTNC SAEHSHPALS CKMAAEFDAF LVSGLRWFCH VDDDNYVNPK
160 170 180 190 200
ALLQLLKTFP QDRDVYVGKP SLNRPIHASE LQSKNRTKLV RFWFATGGAG
210 220 230 240 250
FCINRQLALK MVPWASGSHF VDTSALIRLP DDCTVGYIIE CKLGGRLQPS
260 270 280 290 300
PLFHSHLETL QLLGAAQLPE QVTLSYGVFE GKLNVIKLPG PFSHEEDPSR
310 320
FRSLHCLLYP DTPWCPLLAA P
Length:321
Mass (Da):36,200
Last modified:July 1, 1997 - v1
Checksum:i76EBCEC2314C50CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94349 mRNA Translation: AAC53260.1
AF015769 mRNA Translation: AAB71669.1
BC010983 mRNA Translation: AAH10983.1
CCDSiCCDS27622.1
RefSeqiNP_032621.1, NM_008595.2
UniGeneiMm.149235

Genome annotation databases

EnsembliENSMUST00000018313; ENSMUSP00000018313; ENSMUSG00000018169
GeneIDi17305
KEGGimmu:17305
UCSCiuc007wri.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

manic fringe homolog (mfng)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94349 mRNA Translation: AAC53260.1
AF015769 mRNA Translation: AAB71669.1
BC010983 mRNA Translation: AAH10983.1
CCDSiCCDS27622.1
RefSeqiNP_032621.1, NM_008595.2
UniGeneiMm.149235

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J0AX-ray1.80A45-321[»]
2J0BX-ray2.10A45-321[»]
ProteinModelPortaliO09008
SMRiO09008
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018313

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

PTM databases

PhosphoSitePlusiO09008

Proteomic databases

PaxDbiO09008
PRIDEiO09008

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018313; ENSMUSP00000018313; ENSMUSG00000018169
GeneIDi17305
KEGGimmu:17305
UCSCiuc007wri.1 mouse

Organism-specific databases

CTDi4242
MGIiMGI:1095404 Mfng

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiO09008
KOiK05948
OMAiNRPIRAS
OrthoDBiEOG091G08XO
PhylomeDBiO09008
TreeFamiTF324207

Enzyme and pathway databases

BRENDAi2.4.1.222 3474

Miscellaneous databases

EvolutionaryTraceiO09008
PROiPR:O09008
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018169 Expressed in 222 organ(s), highest expression level in cerebral cortex
ExpressionAtlasiO09008 baseline and differential
GenevisibleiO09008 MM

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMFNG_MOUSE
AccessioniPrimary (citable) accession number: O09008
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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