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Protein

Nuclear receptor coactivator 3

Gene

Ncoa3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity).By similarity1 Publication

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.

Activity regulationi

Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Acyltransferase, Transferase
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 3 (EC:2.3.1.48)
Short name:
NCoA-3
Alternative name(s):
Amplified in breast cancer-1 protein homolog
Short name:
AIB-1
CBP-interacting protein
Short name:
p/CIP
Short name:
pCIP
Receptor-associated coactivator 3
Short name:
RAC-3
Steroid receptor coactivator protein 3
Short name:
SRC-3
Thyroid hormone receptor activator molecule 1
Short name:
ACTR
Short name:
TRAM-1
Gene namesi
Name:Ncoa3
Synonyms:Aib1, Pcip, Rac3, Tram1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1276535 Ncoa3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects result in diverse phenotype of postnatal growth retardation, such as dwarfism, delayed puberty, abnormal reproductive function and mammary gland growth retardation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000944072 – 1398Nuclear receptor coactivator 3Add BLAST1397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei215PhosphoserineCombined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Modified residuei594Phosphoserine; by CK1By similarity1
Modified residuei609N6-acetyllysineCombined sources1
Modified residuei612N6-acetyllysineCombined sources1
Modified residuei613N6-acetyllysineCombined sources1
Modified residuei680N6-acetyllysineBy similarity1
Modified residuei687PhosphoserineBy similarity1
Modified residuei720PhosphoserineCombined sources1
Modified residuei847PhosphoserineCombined sources1
Modified residuei850PhosphoserineCombined sources1
Modified residuei1040PhosphoserineBy similarity1
Modified residuei1178Asymmetric dimethylarginineCombined sources1
Modified residuei1184Asymmetric dimethylarginineCombined sources1
Modified residuei1195Asymmetric dimethylarginineCombined sources1
Modified residuei1304PhosphoserineBy similarity1

Post-translational modificationi

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function (By similarity).By similarity
Methylated by CARM1.1 Publication
Phosphorylated by IKK complex. Regulated its function (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO09000
PaxDbiO09000
PeptideAtlasiO09000
PRIDEiO09000

PTM databases

iPTMnetiO09000
PhosphoSitePlusiO09000

Expressioni

Tissue specificityi

Not expressed in all steroid sensitive tissues. Highly expressed in the female reproductive system, in both oocyte and smooth muscle cells of the oviduct, but not expressed in the uterine endometrium. Highly expressed in mammary glands. Expressed moderately in smooth muscle cells of both blood vessels and intestines, and weakly expressed in hepatocytes. In brain, highly expressed in neurons of the hyppocampus, and in mitral cell and granule layers of the olfactory bulb. Expressed moderately in the internal layer of cerebellum. Not expressed in the spinal chord, cardiac muscle, skeletal muscle, thymus and pancreas.1 Publication

Gene expression databases

CleanExiMM_NCOA3
MM_RAC3

Interactioni

Subunit structurei

Interacts with the histone acetyltransferase protein CREBBP. These two proteins are present in a complex containing NCOA2, IKKA, IKKB and IKBKG. Interacts with PCAF and NR3C1 (By similarity). Interacts with CARM1. Interacts with CASP8AP2. Interacts with ATAD2 and this interaction is enhanced by estradiol (By similarity). Interacts with PSMB9. Binds to CSNK1D (By similarity). Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with NPAS2 (By similarity). Interacts with NR4A3 (via AF-1 domain) (PubMed:12709428). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (PubMed:23019124).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-44921N
IntActiO09000, 15 interactors
STRINGi10090.ENSMUSP00000085416

Structurei

3D structure databases

ProteinModelPortaliO09000
SMRiO09000
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 83bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini111 – 181PASPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni949 – 1113Interaction with CREBBPBy similarityAdd BLAST165
Regioni1104 – 1278AcetyltransferaseAdd BLAST175

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi678 – 682LXXLL motif 15
Motifi730 – 734LXXLL motif 25
Motifi1041 – 1045LXXLL motif 35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi429 – 664Ser-richAdd BLAST236
Compositional biasi965 – 987Poly-GlnAdd BLAST23

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain) (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3561 Eukaryota
ENOG410XRJI LUCA
HOGENOMiHOG000230947
HOVERGENiHBG052583
InParanoidiO09000

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.10.287.1070, 1 hit
4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR010011 DUF1518
IPR032565 DUF4927
IPR036638 HLH_DNA-bd_sf
IPR028818 NCOA3
IPR009110 Nuc_rcpt_coact
IPR014920 Nuc_rcpt_coact_Ncoa-typ
IPR037077 Nuc_rcpt_coact_Ncoa_int_sf
IPR017426 Nuclear_rcpt_coactivator
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR014935 SRC/p160_LXXLL
PANTHERiPTHR10684 PTHR10684, 1 hit
PTHR10684:SF3 PTHR10684:SF3, 1 hit
PfamiView protein in Pfam
PF07469 DUF1518, 1 hit
PF16279 DUF4927, 1 hit
PF08815 Nuc_rec_co-act, 1 hit
PF00989 PAS, 1 hit
PF08832 SRC-1, 1 hit
PIRSFiPIRSF038181 Nuclear_receptor_coactivator, 1 hit
SMARTiView protein in SMART
SM01151 DUF1518, 1 hit
SM00353 HLH, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
SSF69125 SSF69125, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O09000-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGLGESSLD PLAAESRKRK LPCDAPGQGL VYSGEKWRRE QESKYIEELA
60 70 80 90 100
ELISANLSDI DNFNVKPDKC AILKETVRQI RQIKEQGKTI SSDDDVQKAD
110 120 130 140 150
VSSTGQGVID KDSLGPLLLQ ALDGFLFVVN RDGNIVFVSE NVTQYLQYKQ
160 170 180 190 200
EDLVNTSVYS ILHEPRRKDF LNTYQNPQLM EFLGLMRTRD KKAPYILIVR
210 220 230 240 250
MLMKTHDILE DVNASPETRQ RYETMQCFAL SQPRAMLEEG EDLQCCMICV
260 270 280 290 300
ARRVTAPFPS SPESFITRHD LSGKVVNIDT NSLRSSMRPG FEDIIRRCIQ
310 320 330 340 350
RFFSLNDGQS WSQKRHYQEA YVHGHAETPV YRFSLADGTI VSAQTKSKLF
360 370 380 390 400
RNPVTNDRHG FISTHFLQRE QNGYRPNPIP QDKGIRPPAA GCGVSMSPNQ
410 420 430 440 450
NVQMMGSRTY GVPDPSNTGQ MGGARYGASS SVASLTPGQS LQSPSSYQNS
460 470 480 490 500
SYGLSMSSPP HGSPGLGPNQ QNIMISPRNR GSPKMASHQF SPAAGAHSPM
510 520 530 540 550
GPSGNTGSHS FSSSSLSALQ AISEGVGTSL LSTLSSPGPK LDNSPNMNIS
560 570 580 590 600
QPSKVSGQDS KSPLGLYCEQ NPVESSVCQS NSRDPQVKKE SKESSGEVSE
610 620 630 640 650
TPRGPLESKG HKKLLQLLTC SSDDRGHSSL TNSPLDPNCK DSSVSVTSPS
660 670 680 690 700
GVSSSTSGTV SSTSNVHGSL LQEKHRILHK LLQNGNSPAE VAKITAEATG
710 720 730 740 750
KDTSSTASCG EGTTRQEQLS PKKKENNALL RYLLDRDDPS DVLAKELQPQ
760 770 780 790 800
ADSGDSKLSQ CSCSTNPSSG QEKDPKIKTE TNDEVSGDLD NLDAILGDLT
810 820 830 840 850
SSDFYNNPTN GGHPGAKQQM FAGPSSLGLR SPQPVQSVRP PYNRAVSLDS
860 870 880 890 900
PVSVGSGPPV KNVSAFPGLP KQPILAGNPR MMDSQENYGA NMGPNRNVPV
910 920 930 940 950
NPTSSPGDWG LANSRASRME PLASSPLGRT GADYSATLPR PAMGGSVPTL
960 970 980 990 1000
PLRSNRLPGA RPSLQQQQQQ QQQQQQQQQQ QQQQQQQMLQ MRTGEIPMGM
1010 1020 1030 1040 1050
GVNPYSPAVQ SNQPGSWPEG MLSMEQGPHG SQNRPLLRNS LDDLLGPPSN
1060 1070 1080 1090 1100
AEGQSDERAL LDQLHTFLSN TDATGLEEID RALGIPELVN QGQALESKQD
1110 1120 1130 1140 1150
VFQGQEAAVM MDQKAALYGQ TYPAQGPPLQ GGFNLQGQSP SFNSMMGQIS
1160 1170 1180 1190 1200
QQGSFPLQGM HPRAGLVRPR TNTPKQLRMQ LQQRLQGQQF LNQSRQALEM
1210 1220 1230 1240 1250
KMENPAGTAV MRPMMPQAFF NAQMAAQQKR ELMSHHLQQQ RMAMMMSQPQ
1260 1270 1280 1290 1300
PQAFSPPPNV TASPSMDGVL AGSAMPQAPP QQFPYPANYG MGQPPEPAFG
1310 1320 1330 1340 1350
RGSSPPSAMM SSRMGPSQNA MVQHPQPTPM YQPSDMKGWP SGNLARNGSF
1360 1370 1380 1390
PQQQFAPQGN PAAYNMVHMN SSGGHLGQMA MTPMPMSGMP MGPDQKYC
Length:1,398
Mass (Da):151,574
Last modified:August 1, 1998 - v2
Checksum:iEF44E92735816C24
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q05BA5Q05BA5_MOUSE
Nuclear receptor coactivator
Ncoa3
1,403Annotation score:
A2A468A2A468_MOUSE
Nuclear receptor coactivator
Ncoa3 mCG_14623
1,402Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000581 mRNA Translation: AAC05020.1
AK021229 mRNA No translation available.
UniGeneiMm.469663

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000581 mRNA Translation: AAC05020.1
AK021229 mRNA No translation available.
UniGeneiMm.469663

3D structure databases

ProteinModelPortaliO09000
SMRiO09000
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-44921N
IntActiO09000, 15 interactors
STRINGi10090.ENSMUSP00000085416

PTM databases

iPTMnetiO09000
PhosphoSitePlusiO09000

Proteomic databases

EPDiO09000
PaxDbiO09000
PeptideAtlasiO09000
PRIDEiO09000

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1276535 Ncoa3

Phylogenomic databases

eggNOGiKOG3561 Eukaryota
ENOG410XRJI LUCA
HOGENOMiHOG000230947
HOVERGENiHBG052583
InParanoidiO09000

Miscellaneous databases

ChiTaRSiNcoa3 mouse
PROiPR:O09000
SOURCEiSearch...

Gene expression databases

CleanExiMM_NCOA3
MM_RAC3

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.10.287.1070, 1 hit
4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR010011 DUF1518
IPR032565 DUF4927
IPR036638 HLH_DNA-bd_sf
IPR028818 NCOA3
IPR009110 Nuc_rcpt_coact
IPR014920 Nuc_rcpt_coact_Ncoa-typ
IPR037077 Nuc_rcpt_coact_Ncoa_int_sf
IPR017426 Nuclear_rcpt_coactivator
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR014935 SRC/p160_LXXLL
PANTHERiPTHR10684 PTHR10684, 1 hit
PTHR10684:SF3 PTHR10684:SF3, 1 hit
PfamiView protein in Pfam
PF07469 DUF1518, 1 hit
PF16279 DUF4927, 1 hit
PF08815 Nuc_rec_co-act, 1 hit
PF00989 PAS, 1 hit
PF08832 SRC-1, 1 hit
PIRSFiPIRSF038181 Nuclear_receptor_coactivator, 1 hit
SMARTiView protein in SMART
SM01151 DUF1518, 1 hit
SM00353 HLH, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
SSF69125 SSF69125, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCOA3_MOUSE
AccessioniPrimary (citable) accession number: O09000
Secondary accession number(s): Q9CRD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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