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Protein

Pleckstrin homology-like domain family A member 2

Gene

Phlda2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids.1 Publication

Miscellaneous

The Phlda2 locus is imprinted. Loss of imprinting results in overexpression, leading to placental growth retardation phenotypes.

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology-like domain family A member 2
Alternative name(s):
Imprinted in placenta and liver protein
Protein 50C15
Gene namesi
Name:Phlda2
Synonyms:Ipl, Tssc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1202307 Phlda2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable, and show overgrowth of placentas with expansion of the spongiotrophoblast. These larger placentas do not confer a fetal growth advantage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27R → L: Impairs the ability to rescue growth in cdc25ts mutant yeasts. 1 Publication1
Mutagenesisi38K → A: Impairs the ability to rescue growth in cdc25ts mutant yeasts. 1 Publication1
Mutagenesisi39R → A: Impairs the ability to rescue growth in cdc25ts mutant yeasts. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539011 – 144Pleckstrin homology-like domain family A member 2Add BLAST144

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08969
PeptideAtlasiO08969
PRIDEiO08969

PTM databases

PhosphoSitePlusiO08969

Expressioni

Tissue specificityi

Specifically expressed at high levels in extraembryonic tissues in the developing conceptus (at protein level). Expressed in placenta and yolc sac. Expressed at low levels in fetal liver and kidney.1 Publication

Developmental stagei

Expressed at high levels in the very early conceptus limited to polar trophectoderm. Strongly expressed in the ectoplacental cone shortly after implantation at 5.5 dpc, and the high expression remains restricted to the extraembryonic tissues at later stages. By 10.5 dpc expression is restricted to the labyrinthine trophoblast and the endodermal component of the visceral yolk sac. Between 12.5 and 14.4 dpc expression in placenta decreases, due to a number of expressing cells. On day 12 of gestation, the abundant expressing cells are trophoblast at the chorionic plate, and clustered type II trophoblast deeper in the labyrinth. Less expressed by terminally differentiated trophoblast. As early as 14.5 dpc, becomes confined to a monolayer of cells at the chorionic plate and to rare cells in septa that protrude into the labyrinth (at protein level).2 Publications

Inductioni

Maternal Phlda2 allele is activated, while paternal Phlda2 is repressed due to genomic imprinting.1 Publication

Gene expression databases

BgeeiENSMUSG00000010760 Expressed in 112 organ(s), highest expression level in embryo
ExpressionAtlasiO08969 baseline and differential
GenevisibleiO08969 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010904

Structurei

3D structure databases

ProteinModelPortaliO08969
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 111PHAdd BLAST94

Domaini

The PH domain binds phosphoinositides with a broad specificity. It may compete with the PH domain of some other proteins, thereby interfering with their binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3) (By similarity).By similarity

Sequence similaritiesi

Belongs to the PHLDA2 family.Curated

Phylogenomic databases

eggNOGiENOG410IZC3 Eukaryota
ENOG4111IXF LUCA
GeneTreeiENSGT00440000039564
HOGENOMiHOG000115528
HOVERGENiHBG052730
InParanoidiO08969
OMAiKKVDCVE
OrthoDBiEOG091G0RST
PhylomeDBiO08969
TreeFamiTF332320

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
SMARTiView protein in SMART
SM00233 PH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O08969-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASKIVMSSK TVKTSDEILC EGELEKRSDS LFQVWKKKRC VLTADRLRLF
60 70 80 90 100
SGKTSPAKEL FFHSILKVDC VEHTSKYVYF TIVTNYYKEI DFRCTVESCW
110 120 130 140
NAAITMALID FQNRRALQDF PRYRYQRSES EMPSEPGEQS ALGP
Length:144
Mass (Da):16,727
Last modified:July 1, 1997 - v1
Checksum:i9BD64B97BA22C1A9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140LIM7A0A140LIM7_MOUSE
Pleckstrin homology-like domain fam...
Phlda2
105Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002708 Genomic DNA Translation: AAB86681.1
Y15443 mRNA Translation: CAA75634.1
AK131929 mRNA Translation: BAE20878.1
BC019141 mRNA Translation: AAH19141.1
CCDSiCCDS22042.1
RefSeqiNP_033460.1, NM_009434.2
XP_006508660.1, XM_006508597.2
UniGeneiMm.334344

Genome annotation databases

EnsembliENSMUST00000010904; ENSMUSP00000010904; ENSMUSG00000010760
GeneIDi22113
KEGGimmu:22113
UCSCiuc009kpj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002708 Genomic DNA Translation: AAB86681.1
Y15443 mRNA Translation: CAA75634.1
AK131929 mRNA Translation: BAE20878.1
BC019141 mRNA Translation: AAH19141.1
CCDSiCCDS22042.1
RefSeqiNP_033460.1, NM_009434.2
XP_006508660.1, XM_006508597.2
UniGeneiMm.334344

3D structure databases

ProteinModelPortaliO08969
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010904

PTM databases

PhosphoSitePlusiO08969

Proteomic databases

PaxDbiO08969
PeptideAtlasiO08969
PRIDEiO08969

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010904; ENSMUSP00000010904; ENSMUSG00000010760
GeneIDi22113
KEGGimmu:22113
UCSCiuc009kpj.1 mouse

Organism-specific databases

CTDi7262
MGIiMGI:1202307 Phlda2

Phylogenomic databases

eggNOGiENOG410IZC3 Eukaryota
ENOG4111IXF LUCA
GeneTreeiENSGT00440000039564
HOGENOMiHOG000115528
HOVERGENiHBG052730
InParanoidiO08969
OMAiKKVDCVE
OrthoDBiEOG091G0RST
PhylomeDBiO08969
TreeFamiTF332320

Miscellaneous databases

PROiPR:O08969
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010760 Expressed in 112 organ(s), highest expression level in embryo
ExpressionAtlasiO08969 baseline and differential
GenevisibleiO08969 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
SMARTiView protein in SMART
SM00233 PH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPHLA2_MOUSE
AccessioniPrimary (citable) accession number: O08969
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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