UniProtKB - O08954 (SNAI2_RAT)
Zinc finger protein SNAI2
Snai2
Functioni
Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 128 – 150 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 159 – 181 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 185 – 207 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 213 – 235 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 241 – 264 | C2H2-type 5; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- chromatin binding Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: RGD
- E-box binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- cartilage morphogenesis Source: RGD
- cell migration Source: RGD
- cell migration involved in endocardial cushion formation Source: RGD
- cellular response to epidermal growth factor stimulus Source: RGD
- cellular response to fibroblast growth factor stimulus Source: RGD
- cellular response to ionizing radiation Source: RGD
- desmosome disassembly Source: RGD
- epithelial to mesenchymal transition Source: RGD
- epithelial to mesenchymal transition involved in endocardial cushion formation Source: RGD
- epithelium development Source: RGD
- negative regulation of anoikis Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of cell adhesion involved in substrate-bound cell migration Source: RGD
- negative regulation of cell adhesion mediated by integrin Source: RGD
- negative regulation of chondrocyte differentiation Source: RGD
- negative regulation of DNA damage response, signal transduction by p53 class mediator Source: RGD
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: RGD
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: RGD
- negative regulation of keratinocyte proliferation Source: RGD
- negative regulation of stem cell proliferation Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of vitamin D biosynthetic process Source: RGD
- negative regulation of vitamin D receptor signaling pathway Source: RGD
- neural crest cell development Source: RGD
- Notch signaling pathway Source: RGD
- osteoblast differentiation Source: RGD
- pigmentation Source: RGD
- positive regulation of cell migration Source: RGD
- positive regulation of fat cell differentiation Source: RGD
- positive regulation of histone acetylation Source: RGD
- regulation of bicellular tight junction assembly Source: RGD
- regulation of branching involved in salivary gland morphogenesis Source: RGD
- regulation of chemokine production Source: RGD
- regulation of osteoblast differentiation Source: RGD
- regulation of transcription, DNA-templated Source: GO_Central
- response to radiation Source: RGD
- roof of mouth development Source: RGD
- sensory perception of sound Source: RGD
- substrate-dependent cell migration Source: RGD
- white fat cell differentiation Source: RGD
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein SNAI2Alternative name(s): Neural crest transcription factor Slug Protein snail homolog 2 |
Gene namesi | Name:Snai2 Synonyms:Slug, Slugh |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3722, Snai2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions (By similarity).By similarity
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047034 | 1 – 268 | Zinc finger protein SNAI2Add BLAST | 268 |
Post-translational modificationi
Proteomic databases
PaxDbi | O08954 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000047699, Expressed in liver and 19 other tissues |
Genevisiblei | O08954, RN |
Interactioni
Subunit structurei
Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity).
Interacts (via zinc fingers) with KPNA2, KPNB1 and TNPO1. May interact (via zinc fingers) with IPO7 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064195 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | SNAG domainBy similarityAdd BLAST | 20 | |
Regioni | 84 – 116 | DisorderedSequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 84 – 101 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 102 – 116 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 128 – 150 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 159 – 181 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 185 – 207 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 213 – 235 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 241 – 264 | C2H2-type 5; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154681 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | O08954 |
OMAi | SGCCIAH |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O08954 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 5 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLCESYPMP VIPKPEILTS
60 70 80 90 100
GAYSPITVWT SAVPFHSPLP SGLSPLTGYS SSLGRVSPLP SSDTSSKDHS
110 120 130 140 150
GSESPISDEE ERLQPKLSDP HAIEAEKFQC NLCNKTYSTF SGLAKHKQLH
160 170 180 190 200
CDAQARKSFS CKYCDKEYVS LGALKMHIRT HTLPCVCKIC GKAFSRPWLL
210 220 230 240 250
QGHIRTHTGE KPFSCPHCNR AFADRSNLRA HLQTHSDVKK YQCKNCSKTF
260
SRMSLLHKHE ESGCCVAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF497973 mRNA Translation: AAM19227.1 U97061 mRNA Translation: AAB58706.1 |
RefSeqi | NP_037167.1, NM_013035.1 |
Genome annotation databases
Ensembli | ENSRNOT00000073049; ENSRNOP00000064195; ENSRNOG00000047699 |
GeneIDi | 25554 |
KEGGi | rno:25554 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF497973 mRNA Translation: AAM19227.1 U97061 mRNA Translation: AAB58706.1 |
RefSeqi | NP_037167.1, NM_013035.1 |
3D structure databases
SMRi | O08954 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064195 |
Proteomic databases
PaxDbi | O08954 |
Genome annotation databases
Ensembli | ENSRNOT00000073049; ENSRNOP00000064195; ENSRNOG00000047699 |
GeneIDi | 25554 |
KEGGi | rno:25554 |
Organism-specific databases
CTDi | 6591 |
RGDi | 3722, Snai2 |
Phylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154681 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | O08954 |
OMAi | SGCCIAH |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O08954 |
Miscellaneous databases
PROi | PR:O08954 |
Gene expression databases
Bgeei | ENSRNOG00000047699, Expressed in liver and 19 other tissues |
Genevisiblei | O08954, RN |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 5 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SNAI2_RAT | |
Accessioni | O08954Primary (citable) accession number: O08954 Secondary accession number(s): Q8R482 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 6, 2005 | |
Last modified: | September 29, 2021 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families