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Protein

Fatty-acid amide hydrolase 1

Gene

Faah

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (By similarity).By similarity

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142Charge relay systemBy similarity1
Binding sitei191Substrate; via carbonyl oxygenBy similarity1
Active sitei217Charge relay systemBy similarity1
Binding sitei217SubstrateBy similarity1
Active sitei241Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.1.4 3474
3.5.1.99 3474
ReactomeiR-MMU-2142753 Arachidonic acid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty-acid amide hydrolase 1 (EC:3.5.1.99)
Alternative name(s):
Anandamide amidohydrolase 1
Oleamide hydrolase 1
Gene namesi
Name:Faah
Synonyms:Faah1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:109609 Faah

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 403CytoplasmicBy similarityAdd BLAST374
Intramembranei404 – 433By similarityAdd BLAST30
Topological domaini434 – 579CytoplasmicBy similarityAdd BLAST146

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3455

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001052651 – 579Fatty-acid amide hydrolase 1Add BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei241PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO08914
MaxQBiO08914
PaxDbiO08914
PeptideAtlasiO08914
PRIDEiO08914

PTM databases

iPTMnetiO08914
PhosphoSitePlusiO08914
SwissPalmiO08914

Expressioni

Gene expression databases

BgeeiENSMUSG00000034171 Expressed in 217 organ(s), highest expression level in colon
CleanExiMM_FAAH
GenevisibleiO08914 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041543

Chemistry databases

BindingDBiO08914

Structurei

3D structure databases

ProteinModelPortaliO08914
SMRiO08914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 241Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1212 Eukaryota
COG0154 LUCA
GeneTreeiENSGT00550000074673
HOGENOMiHOG000016500
HOVERGENiHBG005632
InParanoidiO08914
KOiK15528
OMAiMPGQEGV
OrthoDBiEOG091G05JU
PhylomeDBiO08914
TreeFamiTF314455

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR030560 FAAH
PANTHERiPTHR11895 PTHR11895, 1 hit
PTHR11895:SF91 PTHR11895:SF91, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

O08914-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLSEVWTAL SGLSGVCLAC SLLSAAVVLR WTRSQTARGA VTRARQKQRA
60 70 80 90 100
GLETMDKAVQ RFRLQNPDLD SEALLALPLL QLVQKLQSGE LSPEAVLFTY
110 120 130 140 150
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH
160 170 180 190 200
ASTLGLSLNE GVTSESDCVV VQVLKLQGAV PFVHTNVPQS MLSYDCSNPL
210 220 230 240 250
FGQTMNPWKP SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG
260 270 280 290 300
ICGLKPTGNR LSKSGLKSCV YGQTAVQLSV GPMARDVDSL ALCMKALLCE
310 320 330 340 350
DLFRLDSTIP PLPFREEIYR SSRPLRVGYY ETDNYTMPTP AMRRAVMETK
360 370 380 390 400
QSLEAAGHTL VPFLPNNIPY ALEVLSAGGL FSDGGCSFLQ NFKGDFVDPC
410 420 430 440 450
LGDLVLVLKL PRWFKKLLSF LLKPLFPRLA AFLNSMCPRS AEKLWELQHE
460 470 480 490 500
IEMYRQSVIA QWKAMNLDVV LTPMLGPALD LNTPGRATGA ISYTVLYNCL
510 520 530 540 550
DFPAGVVPVT TVTAEDDAQM EHYKGYFGDM WDNILKKGMK KGIGLPVAVQ
560 570
CVALPWQEEL CLRFMREVER LMTPEKRPS
Length:579
Mass (Da):63,221
Last modified:July 1, 1997 - v1
Checksum:i11B2DF6CBC110015
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72E → Q in AAH06863 (PubMed:15489334).Curated1
Sequence conflicti230S → P in AK004985 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82536 mRNA Translation: AAB58506.1
AF098009
, AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA Translation: AAD11788.1
AK004985 mRNA No translation available.
BC006863 mRNA Translation: AAH06863.1
BC052321 mRNA Translation: AAH52321.1
CCDSiCCDS18501.1
RefSeqiNP_034303.3, NM_010173.4
UniGeneiMm.256025

Genome annotation databases

EnsembliENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171
GeneIDi14073
KEGGimmu:14073
UCSCiuc008ufs.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82536 mRNA Translation: AAB58506.1
AF098009
, AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA Translation: AAD11788.1
AK004985 mRNA No translation available.
BC006863 mRNA Translation: AAH06863.1
BC052321 mRNA Translation: AAH52321.1
CCDSiCCDS18501.1
RefSeqiNP_034303.3, NM_010173.4
UniGeneiMm.256025

3D structure databases

ProteinModelPortaliO08914
SMRiO08914
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041543

Chemistry databases

BindingDBiO08914
ChEMBLiCHEMBL3455

PTM databases

iPTMnetiO08914
PhosphoSitePlusiO08914
SwissPalmiO08914

Proteomic databases

EPDiO08914
MaxQBiO08914
PaxDbiO08914
PeptideAtlasiO08914
PRIDEiO08914

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171
GeneIDi14073
KEGGimmu:14073
UCSCiuc008ufs.2 mouse

Organism-specific databases

CTDi2166
MGIiMGI:109609 Faah

Phylogenomic databases

eggNOGiKOG1212 Eukaryota
COG0154 LUCA
GeneTreeiENSGT00550000074673
HOGENOMiHOG000016500
HOVERGENiHBG005632
InParanoidiO08914
KOiK15528
OMAiMPGQEGV
OrthoDBiEOG091G05JU
PhylomeDBiO08914
TreeFamiTF314455

Enzyme and pathway databases

BRENDAi3.5.1.4 3474
3.5.1.99 3474
ReactomeiR-MMU-2142753 Arachidonic acid metabolism

Miscellaneous databases

PROiPR:O08914
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034171 Expressed in 217 organ(s), highest expression level in colon
CleanExiMM_FAAH
GenevisibleiO08914 MM

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR030560 FAAH
PANTHERiPTHR11895 PTHR11895, 1 hit
PTHR11895:SF91 PTHR11895:SF91, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFAAH1_MOUSE
AccessioniPrimary (citable) accession number: O08914
Secondary accession number(s): Q922S0, Q9DBF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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