UniProtKB - O08914 (FAAH1_MOUSE)
Protein
Fatty-acid amide hydrolase 1
Gene
Faah
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the hydrolysis of endogenous amidated lipids like the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides such as the taurine-conjugated fatty acids (a structural class of central nervous system (CNS) metabolites), to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:15533037, PubMed:32271712). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (PubMed:32271712). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (By similarity).By similarity2 Publications
Catalytic activityi
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + ethanolamine2 PublicationsEC:3.5.1.992 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:3.5.1.99By similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + H+ + methanolBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the backward1 Publication direction.
- H2O + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Inhibited the trifluoromethyl compound PF-3845.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 142 | Charge relay systemBy similarity | 1 | |
Binding sitei | 191 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 217 | Charge relay systemBy similarity | 1 | |
Binding sitei | 217 | SubstrateBy similarity | 1 | |
Active sitei | 241 | Acyl-ester intermediateBy similarity | 1 |
GO - Molecular functioni
- acylglycerol lipase activity Source: MGI
- amidase activity Source: MGI
- anandamide amidohydrolase activity Source: UniProtKB
- fatty acid amide hydrolase activity Source: UniProtKB
- hydrolase activity, acting on ester bonds Source: MGI
- identical protein binding Source: MGI
- lipid binding Source: MGI
- oleamide hydrolase activity Source: UniProtKB-EC
- phospholipid binding Source: MGI
GO - Biological processi
- fatty acid catabolic process Source: UniProtKB
- monoacylglycerol catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 3.5.1.4, 3474 3.5.1.99, 3474 |
Reactomei | R-MMU-2142753, Arachidonic acid metabolism |
Chemistry databases
SwissLipidsi | SLP:000001980 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Faah Synonyms:Faah1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109609, Faah |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein By similarity
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass membrane protein By similarity
Note: Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- organelle membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 9 – 29 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 403 | CytoplasmicBy similarityAdd BLAST | 374 | |
Intramembranei | 404 – 433 | By similarityAdd BLAST | 30 | |
Topological domaini | 434 – 579 | CytoplasmicBy similarityAdd BLAST | 146 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Genetic ablation of FAAH bidirectionally dysregulates a subset of intracellular, but not circulating, N-fatty acyl amino acids.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3455 |
DrugCentrali | O08914 |
GuidetoPHARMACOLOGYi | 1400 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105265 | 1 – 579 | Fatty-acid amide hydrolase 1Add BLAST | 579 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 241 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O08914 |
jPOSTi | O08914 |
MaxQBi | O08914 |
PaxDbi | O08914 |
PeptideAtlasi | O08914 |
PRIDEi | O08914 |
PTM databases
iPTMneti | O08914 |
PhosphoSitePlusi | O08914 |
SwissPalmi | O08914 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034171, Expressed in cardiac ventricle and 241 other tissues |
Genevisiblei | O08914, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041543 |
Chemistry databases
BindingDBi | O08914 |
Miscellaneous databases
RNActi | O08914, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 238 – 241 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the amidase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1212, Eukaryota |
GeneTreei | ENSGT00940000161237 |
HOGENOMi | CLU_009600_9_3_1 |
InParanoidi | O08914 |
OMAi | MQPWKYD |
OrthoDBi | 852596at2759 |
PhylomeDBi | O08914 |
TreeFami | TF314455 |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR030560, FAAH |
PANTHERi | PTHR45847:SF3, PTHR45847:SF3, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
i Sequence
Sequence statusi: Complete.
O08914-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLSEVWTAL SGLSGVCLAC SLLSAAVVLR WTRSQTARGA VTRARQKQRA
60 70 80 90 100
GLETMDKAVQ RFRLQNPDLD SEALLALPLL QLVQKLQSGE LSPEAVLFTY
110 120 130 140 150
LGKAWEVNKG TNCVTSYLTD CETQLSQAPR QGLLYGVPVS LKECFSYKGH
160 170 180 190 200
ASTLGLSLNE GVTSESDCVV VQVLKLQGAV PFVHTNVPQS MLSYDCSNPL
210 220 230 240 250
FGQTMNPWKP SKSPGGSSGG EGALIGSGGS PLGLGTDIGG SIRFPSAFCG
260 270 280 290 300
ICGLKPTGNR LSKSGLKSCV YGQTAVQLSV GPMARDVDSL ALCMKALLCE
310 320 330 340 350
DLFRLDSTIP PLPFREEIYR SSRPLRVGYY ETDNYTMPTP AMRRAVMETK
360 370 380 390 400
QSLEAAGHTL VPFLPNNIPY ALEVLSAGGL FSDGGCSFLQ NFKGDFVDPC
410 420 430 440 450
LGDLVLVLKL PRWFKKLLSF LLKPLFPRLA AFLNSMCPRS AEKLWELQHE
460 470 480 490 500
IEMYRQSVIA QWKAMNLDVV LTPMLGPALD LNTPGRATGA ISYTVLYNCL
510 520 530 540 550
DFPAGVVPVT TVTAEDDAQM EHYKGYFGDM WDNILKKGMK KGIGLPVAVQ
560 570
CVALPWQEEL CLRFMREVER LMTPEKRPS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | E → Q in AAH06863 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 230 | S → P in AK004985 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82536 mRNA Translation: AAB58506.1 AF098009 , AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA Translation: AAD11788.1 AK004985 mRNA No translation available. BC006863 mRNA Translation: AAH06863.1 BC052321 mRNA Translation: AAH52321.1 |
CCDSi | CCDS18501.1 |
RefSeqi | NP_034303.3, NM_010173.4 |
Genome annotation databases
Ensembli | ENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171 |
GeneIDi | 14073 |
KEGGi | mmu:14073 |
UCSCi | uc008ufs.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82536 mRNA Translation: AAB58506.1 AF098009 , AF097997, AF097998, AF097999, AF098000, AF098001, AF098002, AF098003, AF098004, AF098005, AF098006, AF098007, AF098008 Genomic DNA Translation: AAD11788.1 AK004985 mRNA No translation available. BC006863 mRNA Translation: AAH06863.1 BC052321 mRNA Translation: AAH52321.1 |
CCDSi | CCDS18501.1 |
RefSeqi | NP_034303.3, NM_010173.4 |
3D structure databases
SMRi | O08914 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000041543 |
Chemistry databases
BindingDBi | O08914 |
ChEMBLi | CHEMBL3455 |
DrugCentrali | O08914 |
GuidetoPHARMACOLOGYi | 1400 |
SwissLipidsi | SLP:000001980 |
PTM databases
iPTMneti | O08914 |
PhosphoSitePlusi | O08914 |
SwissPalmi | O08914 |
Proteomic databases
EPDi | O08914 |
jPOSTi | O08914 |
MaxQBi | O08914 |
PaxDbi | O08914 |
PeptideAtlasi | O08914 |
PRIDEi | O08914 |
Protocols and materials databases
Antibodypediai | 1478, 375 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049095; ENSMUSP00000041543; ENSMUSG00000034171 |
GeneIDi | 14073 |
KEGGi | mmu:14073 |
UCSCi | uc008ufs.2, mouse |
Organism-specific databases
CTDi | 2166 |
MGIi | MGI:109609, Faah |
Phylogenomic databases
eggNOGi | KOG1212, Eukaryota |
GeneTreei | ENSGT00940000161237 |
HOGENOMi | CLU_009600_9_3_1 |
InParanoidi | O08914 |
OMAi | MQPWKYD |
OrthoDBi | 852596at2759 |
PhylomeDBi | O08914 |
TreeFami | TF314455 |
Enzyme and pathway databases
BRENDAi | 3.5.1.4, 3474 3.5.1.99, 3474 |
Reactomei | R-MMU-2142753, Arachidonic acid metabolism |
Miscellaneous databases
BioGRID-ORCSi | 14073, 1 hit in 17 CRISPR screens |
ChiTaRSi | Faah, mouse |
PROi | PR:O08914 |
RNActi | O08914, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034171, Expressed in cardiac ventricle and 241 other tissues |
Genevisiblei | O08914, MM |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR030560, FAAH |
PANTHERi | PTHR45847:SF3, PTHR45847:SF3, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FAAH1_MOUSE | |
Accessioni | O08914Primary (citable) accession number: O08914 Secondary accession number(s): Q922S0, Q9DBF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families