UniProtKB - O08878 (GPER1_RAT)
G-protein coupled estrogen receptor 1
Gper1
Functioni
GO - Molecular functioni
- chromatin binding Source: RGD
- drug binding Source: RGD
- estrogen receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: UniProtKB-KW
- hormone binding Source: RGD
- steroid binding Source: RGD
- steroid hormone binding Source: UniProtKB
- steroid hormone receptor activity Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- apoptotic chromosome condensation Source: UniProtKB
- cell cycle Source: UniProtKB-KW
- cellular response to estradiol stimulus Source: UniProtKB
- cellular response to glucose stimulus Source: UniProtKB
- cellular response to mineralocorticoid stimulus Source: UniProtKB
- cellular response to peptide hormone stimulus Source: UniProtKB
- cellular response to tumor necrosis factor Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: RGD
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- intracellular steroid hormone receptor signaling pathway Source: RGD
- modulation of chemical synaptic transmission Source: RGD
- negative regulation of cell cycle arrest Source: UniProtKB
- negative regulation of cell cycle process Source: RGD
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of DNA metabolic process Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: RGD
- negative regulation of fat cell differentiation Source: UniProtKB
- negative regulation of gene expression Source: UniProtKB
- negative regulation of inflammatory response Source: UniProtKB
- negative regulation of leukocyte activation Source: UniProtKB
- negative regulation of lipid biosynthetic process Source: UniProtKB
- negative regulation of protein kinase B signaling Source: RGD
- negative regulation of vascular associated smooth muscle cell proliferation Source: RGD
- neuronal action potential Source: UniProtKB
- nuclear fragmentation involved in apoptotic nuclear change Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of blood vessel diameter Source: UniProtKB
- positive regulation of cardiac vascular smooth muscle cell differentiation Source: RGD
- positive regulation of cell migration Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: UniProtKB
- positive regulation of endothelial cell apoptotic process Source: UniProtKB
- positive regulation of epidermal growth factor receptor signaling pathway Source: RGD
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of gene expression Source: UniProtKB
- positive regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- positive regulation of inositol trisphosphate biosynthetic process Source: UniProtKB
- positive regulation of insulin secretion Source: UniProtKB
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of neurogenesis Source: UniProtKB
- positive regulation of neurotransmitter secretion Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase signaling Source: RGD
- positive regulation of protein localization to plasma membrane Source: UniProtKB
- positive regulation of protein phosphorylation Source: UniProtKB
- positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of uterine smooth muscle contraction Source: UniProtKB
- regulation of cytosolic calcium ion concentration Source: UniProtKB
- steroid hormone mediated signaling pathway Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Apoptosis, Cell cycle, Differentiation, Immunity, Inflammatory response, Innate immunity, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: G-protein coupled estrogen receptor 1Alternative name(s): Chemoattractant receptor-like 2 G protein-coupled estrogen receptor 1 G-protein coupled receptor 301 Publication G-protein coupled receptor 41 Membrane estrogen receptor Short name: mER |
Gene namesi | Name:Gper1 Synonyms:Cmkrl2, Gper, Gpr301 Publication, Gpr41 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619845, Gper1 |
Subcellular locationi
Endosome
- Early endosome By similarity
- Recycling endosome By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion membrane 1 Publication; Multi-pass membrane protein
Cytoskeleton
- cytoskeleton 1 Publication
Plasma membrane
Golgi apparatus
- trans-Golgi network By similarity
- Golgi apparatus membrane 2 Publications; Multi-pass membrane protein
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein By similarity
Other locations
- perinuclear region By similarity
- Cytoplasm 1 Publication
- Cytoplasmic vesicle membrane By similarity; Multi-pass membrane protein By similarity
- dendrite
- axon 1 Publication
- postsynaptic density 2 Publications
Note: Endocytosed in an agonist- and arrestin-independent manner. Colocalized with RAMP3 and clathrin-coated pits at the plasma membrane. Colocalized with transferrin receptor at the plasma membrane and perinuclear region. Accumulated and colocalized with RAB11 proteins in recycling endosomes and trans-Golgi network (TGN), but does neither recycle back to the cell surface nor traffics to late endosome or lysosome. Colocalized with calnexin in the endoplasmic reticulum. Traffics to intracellular sites via cytokeratin intermediate filaments like KRT7 and KRT8 after constitutive endocytosis in epithelial cells. Colocalized with EGFR in the nucleus of agonist-induced cancer-associated fibroblasts (CAF) (By similarity). Colocalized with BSN to the active zone of presynaptic density. Colocalized with DLG4/PSD95 and neurabin-2 PPP1R9B in neuronal synaptosomes.By similarity
Cytoskeleton
- keratin filament Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Endosome
- early endosome Source: UniProtKB
- recycling endosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
- trans-Golgi network Source: UniProtKB
Mitochondrion
- mitochondrial membrane Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- dendritic spine membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- presynaptic membrane Source: UniProtKB
Other locations
- axon Source: UniProtKB
- axon terminus Source: UniProtKB
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle membrane Source: UniProtKB
- dendrite Source: UniProtKB
- dendritic shaft Source: UniProtKB
- dendritic spine head Source: UniProtKB
- hippocampal mossy fiber to CA3 synapse Source: RGD
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: UniProtKB
- postsynaptic density Source: UniProtKB
- presynaptic active zone Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 62 | ExtracellularSequence analysisAdd BLAST | 62 | |
Transmembranei | 63 – 84 | Helical; Name=1Sequence analysisAdd BLAST | 22 | |
Topological domaini | 85 – 96 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 97 – 120 | Helical; Name=2Sequence analysisAdd BLAST | 24 | |
Topological domaini | 121 – 132 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 133 – 153 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 154 – 175 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 176 – 194 | Helical; Name=4Sequence analysisAdd BLAST | 19 | |
Topological domaini | 195 – 220 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 221 – 236 | Helical; Name=5Sequence analysisAdd BLAST | 16 | |
Topological domaini | 237 – 259 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 260 – 280 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 281 – 306 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 307 – 327 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 328 – 375 | CytoplasmicSequence analysisAdd BLAST | 48 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069312 | 1 – 375 | G-protein coupled estrogen receptor 1Add BLAST | 375 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 130 ↔ 207 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, Ubl conjugationProteomic databases
PaxDbi | O08878 |
PTM databases
GlyGeni | O08878, 2 sites |
PhosphoSitePlusi | O08878 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer (Probable). Heterodimer; heterodimerizes with other G-protein-coupled receptor (GPCRs) like CRHR1, HTR1A and PAQR8.
Interacts with RAMP3.
Interacts with KRT7 and KRT8.
Interacts with EGFR; the interaction increases after agonist-induced stimulation in cancer-associated fibroblasts (CAF).
Interacts with EGFR and ESR1 (By similarity).
Interacts (via C-terminus tail motif) with DLG4 (via N-terminus tandem pair of PDZ domains); the interaction is direct and induces the increase of GPER1 protein levels residing at the plasma membrane surface in a estradiol-independent manner.
By similarityCurated1 PublicationProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000001732 |
Chemistry databases
BindingDBi | O08878 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QU56, Eukaryota |
InParanoidi | O08878 |
OrthoDBi | 736624at2759 |
PhylomeDBi | O08878 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAATTPAQDV GVEIYLGPVW PAPSNSTPLA LNLSLALRED APGNLTGDLS
60 70 80 90 100
EHQQYVIALF LSCLYTIFLF PIGFVGNILI LVVNISFREK MTIPDLYFIN
110 120 130 140 150
LAAADLILVA DSLIEVFNLD EQYYDIAVLC TFMSLFLQIN MYSSVFFLTW
160 170 180 190 200
MSFDRYLALA KAMRCGLFRT KHHARLSCGL IWMASVSATL VPFTAVHLRH
210 220 230 240 250
TEEACFCFAD VREVQWLEVT LGFIVPFAII GLCYSLIVRA LIRAHRHRGL
260 270 280 290 300
RPRRQKALRM IFAVVLVFFI CWLPENVFIS VHLLQWAQPG DTPCKQSFRH
310 320 330 340 350
AYPLTGHIVN LAAFSNSCLS PLIYSFLGET FRDKLRLYVA QKTSLPALNR
360 370
FCHATLKAVI PDSTEQSDVK FSSAV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V654 | G3V654_RAT | G-protein-coupled estrogen receptor... | Gper1 Gper, rCG_42789 | 375 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92802 mRNA Translation: AAC53208.1 |
PIRi | JC5509 |
RefSeqi | NP_598257.1, NM_133573.1 |
Genome annotation databases
GeneIDi | 171104 |
KEGGi | rno:171104 |
UCSCi | RGD:619845, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92802 mRNA Translation: AAC53208.1 |
PIRi | JC5509 |
RefSeqi | NP_598257.1, NM_133573.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000001732 |
Chemistry databases
BindingDBi | O08878 |
ChEMBLi | CHEMBL3309103 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | O08878, 2 sites |
PhosphoSitePlusi | O08878 |
Proteomic databases
PaxDbi | O08878 |
Genome annotation databases
GeneIDi | 171104 |
KEGGi | rno:171104 |
UCSCi | RGD:619845, rat |
Organism-specific databases
CTDi | 2852 |
RGDi | 619845, Gper1 |
Phylogenomic databases
eggNOGi | ENOG502QU56, Eukaryota |
InParanoidi | O08878 |
OrthoDBi | 736624at2759 |
PhylomeDBi | O08878 |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:O08878 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPER1_RAT | |
Accessioni | O08878Primary (citable) accession number: O08878 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries