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Protein

Baculoviral IAP repeat-containing protein 3

Gene

Birc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Activity regulationi

USP19 regulates the stability of BIRC3/c-IAP2 by preventing its ubiquitination.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi290ZincPROSITE-ProRule annotation1
Metal bindingi293ZincPROSITE-ProRule annotation1
Metal bindingi310ZincPROSITE-ProRule annotation1
Metal bindingi317ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri553 – 588RING-typePROSITE-ProRule annotationAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiI32.003

Names & Taxonomyi

Protein namesi
Recommended name:
Baculoviral IAP repeat-containing protein 3 (EC:2.3.2.271 Publication)
Alternative name(s):
Cellular inhibitor of apoptosis 2
Short name:
C-IAP21 Publication
Inhibitor of apoptosis protein 11 Publication
Short name:
mIAP11 Publication
RING-type E3 ubiquitin transferase BIRC3Curated
Gene namesi
Name:Birc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1197007 Birc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223501 – 600Baculoviral IAP repeat-containing protein 3Add BLAST600

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138PhosphoserineBy similarity1

Post-translational modificationi

Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO08863
MaxQBiO08863
PaxDbiO08863
PRIDEiO08863

PTM databases

iPTMnetiO08863
PhosphoSitePlusiO08863

Expressioni

Gene expression databases

CleanExiMM_BIRC2
MM_BIRC3

Interactioni

Subunit structurei

Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). Interaction with TRAF2 is required for ubiquitination of IKBKE, degradation of NFKBIA and activation of NF-kappa-B. Interacts with RIP1, RIP2, RIP3, RIP4 and USP19 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Traf2P394297EBI-642236,EBI-520016

Protein-protein interaction databases

BioGridi198147, 15 interactors
CORUMiO08863
DIPiDIP-43742N
IntActiO08863, 6 interactors
MINTiO08863
STRINGi10090.ENSMUSP00000013949

Structurei

3D structure databases

ProteinModelPortaliO08863
SMRiO08863
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 94BIR 1Add BLAST68
Repeati167 – 233BIR 2Add BLAST67
Repeati253 – 320BIR 3Add BLAST68
Domaini436 – 525CARDPROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the IAP family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri553 – 588RING-typePROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1101 Eukaryota
ENOG410YPNM LUCA
HOGENOMiHOG000232059
HOVERGENiHBG004848
InParanoidiO08863
KOiK16060

Family and domain databases

CDDicd00022 BIR, 3 hits
InterProiView protein in InterPro
IPR001370 BIR_rpt
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
IPR001841 Znf_RING
PfamiView protein in Pfam
PF00653 BIR, 3 hits
PF00619 CARD, 1 hit
SMARTiView protein in SMART
SM00238 BIR, 3 hits
SM00114 CARD, 1 hit
SM00184 RING, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS01282 BIR_REPEAT_1, 3 hits
PS50143 BIR_REPEAT_2, 3 hits
PS50209 CARD, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.iShow all

O08863-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVQDSAFLAK LMKSADTFEL KYDFSCELYR LSTYSAFPRG VPVSERSLAR
60 70 80 90 100
AGFYYTGAND KVKCFCCGLM LDNWKQGDSP MEKHRKLYPS CNFVQTLNPA
110 120 130 140 150
NSLEASPRPS LPSTAMSTMP LSFASSENTG YFSGSYSSFP SDPVNFRANQ
160 170 180 190 200
DCPALSTSPY HFAMNTEKAR LLTYETWPLS FLSPAKLAKA GFYYIGPGDR
210 220 230 240 250
VACFACDGKL SNWERKDDAM SEHQRHFPSC PFLKDLGQSA SRYTVSNLSM
260 270 280 290 300
QTHAARIRTF SNWPSSALVH SQELASAGFY YTGHSDDVKC FCCDGGLRCW
310 320 330 340 350
ESGDDPWVEH AKWFPRCEYL LRIKGQEFVS QVQAGYPHLL EQLLSTSDSP
360 370 380 390 400
EDENADAAIV HFGPGESSED VVMMSTPVVK AALEMGFSRS LVRQTVQWQI
410 420 430 440 450
LATGENYRTV SDLVIGLLDA EDEMREEQME QAAEEEESDD LALIRKNKMV
460 470 480 490 500
LFQHLTCVTP MLYCLLSARA ITEQECNAVK QKPHTLQAST LIDTVLAKGN
510 520 530 540 550
TAATSFRNSL REIDPALYRD IFVQQDIRSL PTDDIAALPM EEQLRKLQEE
560 570 580 590 600
RMCKVCMDRE VSIVFIPCGH LVVCKDCAPS LRKCPICRGT IKGTVRTFLS
Length:600
Mass (Da):67,228
Last modified:July 27, 2011 - v2
Checksum:i2EC9C7B567721382
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2CGA5A2CGA5_MOUSE
Baculoviral IAP repeat-containing p...
Birc3 mCG_9893
602Annotation score:
F7BQL8F7BQL8_MOUSE
Baculoviral IAP repeat-containing p...
Birc3
138Annotation score:
A2CGA3A2CGA3_MOUSE
Baculoviral IAP repeat-containing p...
Birc3
376Annotation score:
E0CZ20E0CZ20_MOUSE
Baculoviral IAP repeat-containing p...
Birc3
168Annotation score:
E0CZ68E0CZ68_MOUSE
Baculoviral IAP repeat-containing p...
Birc3
175Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti398W → R in AAC53531 (PubMed:9441758).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88908 mRNA Translation: AAC53531.1
CT030639 Genomic DNA No translation available.
RefSeqiNP_031490.2, NM_007464.3
UniGeneiMm.2026

Genome annotation databases

GeneIDi11796
KEGGimmu:11796

Similar proteinsi

Entry informationi

Entry nameiBIRC3_MOUSE
AccessioniPrimary (citable) accession number: O08863
Secondary accession number(s): E9QLX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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