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Protein

60 kDa SS-A/Ro ribonucleoprotein

Gene

Trove2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. May stabilize some of these RNAs and protect them from degradation.1 Publication
May play roles in cilia formation and/or maintenance.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi378Divalent metal cationBy similarity1
Metal bindingi380Divalent metal cationBy similarity1
Metal bindingi445Divalent metal cationBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • misfolded RNA binding Source: MGI
  • RNA binding Source: MGI
  • U2 snRNA binding Source: MGI

GO - Biological processi

  • cilium assembly Source: MGI
  • immune system development Source: MGI
  • response to UV Source: MGI
  • smoothened signaling pathway Source: MGI

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processCilium biogenesis/degradation
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa SS-A/Ro ribonucleoprotein
Short name:
60 kDa Ro protein
Short name:
60 kDa ribonucleoprotein Ro
Short name:
RoRNP
Alternative name(s):
TROVE domain family member 2
Gene namesi
Name:Trove2
Synonyms:Ssa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106652 Trove2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cilia absent or reduced, virtually no cilia of the normal 5 µM mean length.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741701 – 53860 kDa SS-A/Ro ribonucleoproteinAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO08848
PaxDbiO08848
PeptideAtlasiO08848
PRIDEiO08848

PTM databases

iPTMnetiO08848
PhosphoSitePlusiO08848

Expressioni

Tissue specificityi

Highest in brain, followed by lung, muscle, kidney and heart. Lower levels are found in testis, liver and spleen.

Gene expression databases

BgeeiENSMUSG00000018199 Expressed in 288 organ(s), highest expression level in frontal cortex
CleanExiMM_TROVE2
ExpressionAtlasiO08848 baseline and differential
GenevisibleiO08848 MM

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Found in a complex with PUF60 and Y5 RNA. Interacts with RIP11 (By similarity).By similarity

Protein-protein interaction databases

BioGridi203507, 2 interactors
IntActiO08848, 3 interactors
MINTiO08848
STRINGi10090.ENSMUSP00000125623

Structurei

3D structure databases

ProteinModelPortaliO08848
SMRiO08848
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 369TROVEPROSITE-ProRule annotationAdd BLAST354

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 284RNA-bindingBy similarityAdd BLAST165
Regioni361 – 538VWFA-like domainBy similarityAdd BLAST178

Domaini

The horseshoe-shaped TROVE domain is built with 7 helical HEAT-like repeats, and is closed by the VWFA-like domain giving rise to a ring-shaped monomer. Single-stranded RNA is bound in the positively charged central cavity (By similarity).By similarity
The MIDAS-like motif in the VWFA-like domain binds divalent metal cations.By similarity

Sequence similaritiesi

Belongs to the Ro 60 kDa family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4465 Eukaryota
ENOG410YCBF LUCA
GeneTreeiENSGT00390000006200
HOGENOMiHOG000075167
HOVERGENiHBG013234
InParanoidiO08848
KOiK11089
OMAiAKQEPML
OrthoDBiEOG091G04MA
PhylomeDBiO08848
TreeFamiTF105990

Family and domain databases

Gene3Di3.40.50.410, 2 hits
InterProiView protein in InterPro
IPR008858 TROVE_dom
IPR037214 TROVE_dom_sf
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF05731 TROVE, 1 hit
SUPFAMiSSF140864 SSF140864, 1 hit
SSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50988 TROVE, 1 hit

Sequencei

Sequence statusi: Complete.

O08848-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEGSANQLQP LSETQVVNSE GGCVWQVTDM NRLRRFLCFG SEGGTYYIKE
60 70 80 90 100
QKLGLENAEA LIRLIEDGRG CEVIQEIKSF SQEGRTAKQE PLLFALAVCS
110 120 130 140 150
QCADINTKQA AFKAVPEVCR IPTHLFTFIQ FKKDLKESMK CGMWGRALRK
160 170 180 190 200
AVADWYNEKG GMAVALVVTK YKQRNGWSHK DLLRLSHLKP SSEGLAIVTK
210 220 230 240 250
YITKGWKEVH EEYKEKALSV EAEKLLKYLE AVEKVKRTKD DLEVIHLIEE
260 270 280 290 300
HQLVREHLLT NHLKSKEVWK ALLQEMPLTA LLRNLGKMTA NSVLEPGNSE
310 320 330 340 350
VSLICEKLSN EKLLKKARIH PFHVLIALET YRAGHGLRGK LKWIPDKDIL
360 370 380 390 400
QALDAAFYTT FKTVEPTGKR FLLAVDVSAS MNQRALGSVL NASTVAAAMC
410 420 430 440 450
MVVTRTEKES SVVAFACDMV PFPVTTDMTL QQVLTAMNKV PAGNTDCSLP
460 470 480 490 500
MIWAQKTDTA ADVFVVFTDN ETFAGQVHPA VALREYRKKM DIPAKLIVCG
510 520 530
MTSNGFTIAD PDDRGMLDMC GFDTAALDVI RNFTLDVI
Length:538
Mass (Da):60,124
Last modified:July 1, 1997 - v1
Checksum:i7850DA35D1726BDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32 – 33RL → V in AAF19049 (PubMed:11196703).Curated2
Sequence conflicti458D → G in AAF19049 (PubMed:11196703).Curated1
Sequence conflicti465V → I in AAF19049 (PubMed:11196703).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66843 mRNA Translation: AAC53142.1
AF065398 Genomic DNA Translation: AAF19049.1
AF042139 Genomic DNA Translation: AAC15667.1
CCDSiCCDS15345.1
RefSeqiNP_038863.1, NM_013835.2
XP_017174940.1, XM_017319451.1
UniGeneiMm.40370

Genome annotation databases

EnsembliENSMUST00000159879; ENSMUSP00000125623; ENSMUSG00000018199
GeneIDi20822
KEGGimmu:20822
UCSCiuc007cxd.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66843 mRNA Translation: AAC53142.1
AF065398 Genomic DNA Translation: AAF19049.1
AF042139 Genomic DNA Translation: AAC15667.1
CCDSiCCDS15345.1
RefSeqiNP_038863.1, NM_013835.2
XP_017174940.1, XM_017319451.1
UniGeneiMm.40370

3D structure databases

ProteinModelPortaliO08848
SMRiO08848
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203507, 2 interactors
IntActiO08848, 3 interactors
MINTiO08848
STRINGi10090.ENSMUSP00000125623

PTM databases

iPTMnetiO08848
PhosphoSitePlusiO08848

Proteomic databases

EPDiO08848
PaxDbiO08848
PeptideAtlasiO08848
PRIDEiO08848

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000159879; ENSMUSP00000125623; ENSMUSG00000018199
GeneIDi20822
KEGGimmu:20822
UCSCiuc007cxd.1 mouse

Organism-specific databases

CTDi6738
MGIiMGI:106652 Trove2

Phylogenomic databases

eggNOGiKOG4465 Eukaryota
ENOG410YCBF LUCA
GeneTreeiENSGT00390000006200
HOGENOMiHOG000075167
HOVERGENiHBG013234
InParanoidiO08848
KOiK11089
OMAiAKQEPML
OrthoDBiEOG091G04MA
PhylomeDBiO08848
TreeFamiTF105990

Miscellaneous databases

PROiPR:O08848
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018199 Expressed in 288 organ(s), highest expression level in frontal cortex
CleanExiMM_TROVE2
ExpressionAtlasiO08848 baseline and differential
GenevisibleiO08848 MM

Family and domain databases

Gene3Di3.40.50.410, 2 hits
InterProiView protein in InterPro
IPR008858 TROVE_dom
IPR037214 TROVE_dom_sf
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF05731 TROVE, 1 hit
SUPFAMiSSF140864 SSF140864, 1 hit
SSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50988 TROVE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRO60_MOUSE
AccessioniPrimary (citable) accession number: O08848
Secondary accession number(s): Q9QYD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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