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UniProtKB - O08838 (AMPH_RAT)
Protein
Amphiphysin
Gene
Amph
Organism
Rattus norvegicus (Rat)
Status
Functioni
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).
By similarityGO - Molecular functioni
- phospholipid binding Source: RGD
- protein-containing complex binding Source: RGD
- protein C-terminus binding Source: RGD
GO - Biological processi
- learning Source: RGD
- positive regulation of endocytosis Source: RGD
- positive regulation of GTPase activity Source: RGD
- synaptic vesicle endocytosis Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Amphiphysin |
Gene namesi | Name:Amph Synonyms:Amph1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620274, Amph |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- synaptic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Plasma Membrane
- leading edge membrane Source: RGD
- plasma membrane Source: GO_Central
Other locations
- axon terminus Source: RGD
- extrinsic component of synaptic vesicle membrane Source: SynGO
- glutamatergic synapse Source: RGD
- photoreceptor ribbon synapse Source: RGD
- presynapse Source: RGD
- presynaptic endocytic zone Source: RGD
- synaptic vesicle Source: RGD
- terminal bouton Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192949 | 1 – 683 | AmphiphysinAdd BLAST | 683 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 252 | PhosphoserineBy similarity | 1 | |
Modified residuei | 260 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 262 | PhosphoserineBy similarity | 1 | |
Modified residuei | 268 | PhosphoserineBy similarity | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 | |
Modified residuei | 276 | PhosphoserineBy similarity | 1 | |
Modified residuei | 280 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 496 | PhosphoserineBy similarity | 1 | |
Modified residuei | 626 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O08838 |
PaxDbi | O08838 |
PRIDEi | O08838 |
PTM databases
iPTMneti | O08838 |
PhosphoSitePlusi | O08838 |
Interactioni
Subunit structurei
Binary interactionsi
O08838
GO - Molecular functioni
- protein C-terminus binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248889, 22 interactors |
CORUMi | O08838 |
DIPi | DIP-30977N |
ELMi | O08838 |
IntActi | O08838, 9 interactors |
MINTi | O08838 |
STRINGi | 10116.ENSRNOP00000017102 |
Structurei
3D structure databases
AlphaFoldDBi | O08838 |
SMRi | O08838 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O08838 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 240 | BARPROSITE-ProRule annotationAdd BLAST | 217 | |
Domaini | 610 – 683 | SH3PROSITE-ProRule annotationAdd BLAST | 74 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 244 – 312 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 421 – 443 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 455 – 599 | DisorderedSequence analysisAdd BLAST | 145 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 10 – 83 | Sequence analysisAdd BLAST | 74 | |
Coiled coili | 144 – 191 | Sequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 287 – 309 | Pro residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 539 – 555 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Keywords - Domaini
Coiled coil, SH3 domainPhylogenomic databases
eggNOGi | KOG3771, Eukaryota |
InParanoidi | O08838 |
OrthoDBi | 1366218at2759 |
PhylomeDBi | O08838 |
Family and domain databases
CDDi | cd12140, SH3_Amphiphysin_I, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR003005, Amphiphysin IPR003017, Amphiphysin_1 IPR035470, Amphiphysin_I_SH3 IPR004148, BAR_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR46514:SF2, PTHR46514:SF2, 1 hit |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF14604, SH3_9, 1 hit |
PRINTSi | PR01251, AMPHIPHYSIN PR01252, AMPHIPHYSIN1 PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O08838-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
60 70 80 90 100
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
110 120 130 140 150
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
160 170 180 190 200
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG
210 220 230 240 250
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFSIQGAPS
260 270 280 290 300
DSGPLRIAKT PSPPEEASPL PSPTASPNHT LAPASPAPVR PRSPSQTRKG
310 320 330 340 350
PPVPPLPKVT PTKELQQENI INFFEDNFVP EINVTTPSQN EVLEVKKEET
360 370 380 390 400
LLDLDFDPFK PDVTPAGSAA ATHSPMSQTL PWDLWTTSTD LVQPASGGSF
410 420 430 440 450
NDFTQPQDTS LFTMQTDQNM AETEQALPTE PQAEEPPTTA AAPTAGLDLG
460 470 480 490 500
LEMEEPKEEA AIPPGTDAGE TVGTEGSTGE EAEAEKAALP AGEGESPEGA
510 520 530 540 550
KIDVESTELA SSESPQAAEL EAGAPQEKVI PSVVIEPASN HEGEEHQETT
560 570 580 590 600
TGTETREATE DVAPQGPAGE KQELATEPTP LDSQAATPAP AGAVDASLSA
610 620 630 640 650
GDAAQELPPG FLYKVETLHD FEAANSDELT LQRGDVVLVV PSDSEADQDA
660 670 680
GWLVGVKESD WLQYRDLATY KGLFPENFTR HLE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JX32 | A0A0G2JX32_RAT | Amphiphysin | Amph LOC100909679, LOC100910792 | 817 | Annotation score: | ||
F1LPP0 | F1LPP0_RAT | Amphiphysin | Amph LOC100909679, LOC100910792 | 656 | Annotation score: | ||
A0A0G2K5Z4 | A0A0G2K5Z4_RAT | Amphiphysin | Amph | 680 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13381 mRNA Translation: CAA73808.1 |
RefSeqi | NP_071553.1, NM_022217.1 |
Genome annotation databases
GeneIDi | 60668 |
KEGGi | rno:60668 |
UCSCi | RGD:620274, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13381 mRNA Translation: CAA73808.1 |
RefSeqi | NP_071553.1, NM_022217.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VJ0 | X-ray | 1.60 | Q | 324-330 | [»] | |
5M5T | X-ray | 1.70 | E/F/G/H/I/J | 349-356 | [»] | |
AlphaFoldDBi | O08838 | |||||
SMRi | O08838 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248889, 22 interactors |
CORUMi | O08838 |
DIPi | DIP-30977N |
ELMi | O08838 |
IntActi | O08838, 9 interactors |
MINTi | O08838 |
STRINGi | 10116.ENSRNOP00000017102 |
PTM databases
iPTMneti | O08838 |
PhosphoSitePlusi | O08838 |
Proteomic databases
jPOSTi | O08838 |
PaxDbi | O08838 |
PRIDEi | O08838 |
Genome annotation databases
GeneIDi | 60668 |
KEGGi | rno:60668 |
UCSCi | RGD:620274, rat |
Organism-specific databases
CTDi | 273 |
RGDi | 620274, Amph |
Phylogenomic databases
eggNOGi | KOG3771, Eukaryota |
InParanoidi | O08838 |
OrthoDBi | 1366218at2759 |
PhylomeDBi | O08838 |
Enzyme and pathway databases
Reactomei | R-RNO-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
EvolutionaryTracei | O08838 |
PROi | PR:O08838 |
Family and domain databases
CDDi | cd12140, SH3_Amphiphysin_I, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR003005, Amphiphysin IPR003017, Amphiphysin_1 IPR035470, Amphiphysin_I_SH3 IPR004148, BAR_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR46514:SF2, PTHR46514:SF2, 1 hit |
Pfami | View protein in Pfam PF03114, BAR, 1 hit PF14604, SH3_9, 1 hit |
PRINTSi | PR01251, AMPHIPHYSIN PR01252, AMPHIPHYSIN1 PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00721, BAR, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51021, BAR, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPH_RAT | |
Accessioni | O08838Primary (citable) accession number: O08838 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2001 |
Last sequence update: | July 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references