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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • cytoskeletal protein binding Source: RGD
  • identical protein binding Source: IntAct

GO - Biological processi

  • actin filament organization Source: InterPro
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • dendritic spine morphogenesis Source: UniProtKB
  • membrane invagination Source: BHF-UCL
  • plasma membrane tubulation Source: BHF-UCL
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  • positive regulation of clathrin-dependent endocytosis Source: BHF-UCL
  • positive regulation of filopodium assembly Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • vesicle budding from membrane Source: BHF-UCL
  • vesicle organization Source: BHF-UCL
  • vesicle transport along actin filament Source: BHF-UCL

Keywordsi

Molecular functionActin-binding
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735144 Wasl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3774296

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001890022 – 501Neural Wiskott-Aldrich syndrome proteinAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei239Phosphoserine; by TNK2By similarity1
Modified residuei253Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication1
Modified residuei304Omega-N-methylarginineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiO08816
PRIDEiO08816

PTM databases

iPTMnetiO08816

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with NOSTRIN, SNX9 and SNX33. Binds to TNK2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with PTK2/FAK1.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • cytoskeletal protein binding Source: RGD
  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-17016N
ELMiO08816
IntActiO08816, 17 interactors
MINTiO08816
STRINGi10116.ENSRNOP00000009261

Chemistry databases

BindingDBiO08816

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni8 – 11Combined sources4
Beta strandi13 – 16Combined sources4
Beta strandi19 – 21Combined sources3
Beta strandi36 – 49Combined sources14
Turni50 – 52Combined sources3
Beta strandi53 – 67Combined sources15
Turni68 – 71Combined sources4
Beta strandi72 – 78Combined sources7
Beta strandi80 – 82Combined sources3
Beta strandi84 – 90Combined sources7
Beta strandi100 – 108Combined sources9
Beta strandi110 – 120Combined sources11
Helixi121 – 137Combined sources17

3D structure databases

ProteinModelPortaliO08816
SMRiO08816
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08816

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 138WH1PROSITE-ProRule annotationAdd BLAST108
Domaini200 – 213CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini401 – 418WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini429 – 446WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 385Pro-richAdd BLAST112
Compositional biasi482 – 501Asp-richAdd BLAST20

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111H4S LUCA
HOGENOMiHOG000214052
HOVERGENiHBG000222
PhylomeDBiO08816

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR030214 N-WASP
IPR011993 PH-like_dom_sf
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF39 PTHR23202:SF39, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 2 hits
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 2 hits
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWSKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYSSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP LSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPASSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFQDDDEWE

D
Length:501
Mass (Da):54,325
Last modified:January 1, 1998 - v2
Checksum:i480E21F26F7FC77E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88461 mRNA Translation: BAA21534.1
UniGeneiRn.126640

Genome annotation databases

UCSCiRGD:735144 rat

Similar proteinsi

Entry informationi

Entry nameiWASL_RAT
AccessioniPrimary (citable) accession number: O08816
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: July 18, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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