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Protein

Protein diaphanous homolog 1

Gene

Diaph1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:15044801, PubMed:18572016, PubMed:23558171). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (PubMed:10678165, PubMed:15044801, PubMed:18572016). Required for cytokinesis, and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:15044801, PubMed:18572016). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165, PubMed:15044801, PubMed:18572016). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (PubMed:15311282). Has neurite outgrowth promoting activity (PubMed:10678165, PubMed:15044801, PubMed:18572016). Acts in a Rho-dependent manner to recruit PFY1 to the membrane (PubMed:9214622). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (By similarity). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity). Required in the control of cell shape (By similarity). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).By similarity6 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • identical protein binding Source: IntAct
  • ion channel binding Source: BHF-UCL
  • profilin binding Source: MGI
  • Rho GTPase binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processHearing

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6785631 ERBB2 Regulates Cell Motility
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein diaphanous homolog 1
Alternative name(s):
Diaphanous-related formin-1
Short name:
DRF1
p140mDIA
Short name:
mDIA11 Publication
Gene namesi
Name:Diaph1
Synonyms:Diap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1194490 Diaph1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice show normal organization of the cerebral cortex with no significant differences in cortical white matter or callosal thickness (PubMed:24781755). Histological analysis of coronal brain sections at early and postnatal stages shows unilateral ventricular enlargement (PubMed:24781755).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948941 – 1255Protein diaphanous homolog 1Add BLAST1255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei22PhosphoserineBy similarity1
Modified residuei751PhosphothreonineBy similarity1
Modified residuei1040N6-acetyllysineBy similarity1
Modified residuei1086N6-acetyllysineBy similarity1
Modified residuei1104PhosphotyrosineCombined sources1
Modified residuei1237PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-751 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO08808
MaxQBiO08808
PaxDbiO08808
PRIDEiO08808

PTM databases

iPTMnetiO08808
PhosphoSitePlusiO08808

Expressioni

Tissue specificityi

Widely expressed. In the organ of Corti, it is expressed at the outer and inner hair cell layers. Expression at the inner hair cell layer is restricted to inner pillar cells. Detected in cochlear spiral ganglion neurons (PubMed:27808407).1 Publication

Developmental stagei

Expressed in the ventricular and subventricular zone progenitor cells of the dorsal and ventral forebrain and the brainstem, at embryonic days E12.5, E14.5, and E17.5. At later embryonic age, it is observed in neurons of the cortex and hippocampus. During postnatal development, expression is detected in the cerebral cortex, basal ganglia, hippocampus, thalamus, and external granular layer of the cerebellum.1 Publication

Gene expression databases

BgeeiENSMUSG00000024456 Expressed in 282 organ(s), highest expression level in conjunctival fornix
CleanExiMM_DIAP1
ExpressionAtlasiO08808 baseline and differential
GenevisibleiO08808 MM

Interactioni

Subunit structurei

Homodimer (PubMed:14992721, PubMed:15864301). Interacts with the GTP-bound form of RHOA (PubMed:9214622). Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC (PubMed:10814512, PubMed:15311282, PubMed:15864301). Interacts with SCAI (PubMed:19350017). Interacts with DCAF7, via FH2 domain (By similarity). Interacts with NCDN (PubMed:18572016). Interacts with OSBPL10, OSBPL2, VIM, TUBB and DYN1 (By similarity).By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199221, 7 interactors
CORUMiO08808
DIPiDIP-29028N
IntActiO08808, 17 interactors
MINTiO08808
STRINGi10090.ENSMUSP00000111297

Structurei

Secondary structure

11255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO08808
SMRiO08808
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08808

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 440GBD/FH3PROSITE-ProRule annotationAdd BLAST366
Domaini586 – 747FH1Add BLAST162
Domaini752 – 1154FH2PROSITE-ProRule annotationAdd BLAST403
Domaini1177 – 1205DADPROSITE-ProRule annotationAdd BLAST29

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili460 – 562Sequence analysisAdd BLAST103
Coiled coili1027 – 1179Sequence analysisAdd BLAST153

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1196 – 1199Arg/Lys-rich (basic)4

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.1 Publication

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1924 Eukaryota
ENOG410Y29H LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000293231
HOVERGENiHBG051357
InParanoidiO08808
KOiK05740
PhylomeDBiO08808

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR014767 DAD_dom
IPR010465 Drf_DAD
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR027653 Formin_Diaph1
IPR009408 Formin_homology_1
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF17 PTHR23213:SF17, 2 hits
PfamiView protein in Pfam
PF06345 Drf_DAD, 1 hit
PF06346 Drf_FH1, 1 hit
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O08808-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPSGGGLGP GRGTRDKKKG RSPDELPATG GDGGKHKKFL ERFTSMRIKK
60 70 80 90 100
EKEKPNSAHR NSSASYGDDP TAQSLQDISD EQVLVLFEQM LVDMNLNEEK
110 120 130 140 150
QQPLREKDIV IKREMVSQYL HTSKAGMNQK ESSRSAMMYI QELRSGLRDM
160 170 180 190 200
HLLSCLESLR VSLNNNPVSW VQTFGAEGLA SLLDILKRLH DEKEETSGNY
210 220 230 240 250
DSRNQHEIIR CLKAFMNNKF GIKTMLETEE GILLLVRAMD PAVPNMMIDA
260 270 280 290 300
AKLLSALCIL PQPEDMNERV LEAMTERAEM DEVERFQPLL DGLKSGTSIA
310 320 330 340 350
LKVGCLQLIN ALITPAEELD FRVHIRSELM RLGLHQVLQE LREIENEDMK
360 370 380 390 400
VQLCVFDEQG DEDFFDLKGR LDDIRMEMDD FGEVFQIILN TVKDSKAEPH
410 420 430 440 450
FLSILQHLLL VRNDYEARPQ YYKLIEECVS QIVLHKNGTD PDFKCRHLQI
460 470 480 490 500
DIERLVDQMI DKTKVEKSEA KATELEKKLD SELTARHELQ VEMKKMENDF
510 520 530 540 550
EQKLQDLQGE KDALDSEKQQ ITAQKQDLEA EVSKLTGEVA KLSKELEDAK
560 570 580 590 600
NEMASLSAVV VAPSVSSSAA VPPAPPLPGD SGTVIPPPPP PPPLPGGVVP
610 620 630 640 650
PSPPLPPGTC IPPPPPLPGG ACIPPPPQLP GSAAIPPPPP LPGVASIPPP
660 670 680 690 700
PPLPGATAIP PPPPLPGATA IPPPPPLPGG TGIPPPPPPL PGSVGVPPPP
710 720 730 740 750
PLPGGPGLPP PPPPFPGAPG IPPPPPGMGV PPPPPFGFGV PAAPVLPFGL
760 770 780 790 800
TPKKVYKPEV QLRRPNWSKF VAEDLSQDCF WTKVKEDRFE NNELFAKLTL
810 820 830 840 850
AFSAQTKTSK AKKDQEGGEE KKSVQKKKVK ELKVLDSKTA QNLSIFLGSF
860 870 880 890 900
RMPYQEIKNV ILEVNEAVLT ESMIQNLIKQ MPEPEQLKML SELKEEYDDL
910 920 930 940 950
AESEQFGVVM GTVPRLRPRL NAILFKLQFS EQVENIKPEI VSVTAACEEL
960 970 980 990 1000
RKSENFSSLL ELTLLVGNYM NAGSRNAGAF GFNISFLCKL RDTKSADQKM
1010 1020 1030 1040 1050
TLLHFLAELC ENDHPEVLKF PDELAHVEKA SRVSAENLQK SLDQMKKQIA
1060 1070 1080 1090 1100
DVERDVQNFP AATDEKDKFV EKMTSFVKDA QEQYNKLRMM HSNMETLYKE
1110 1120 1130 1140 1150
LGDYFVFDPK KLSVEEFFMD LHNFRNMFLQ AVKENQKRRE TEEKMRRAKL
1160 1170 1180 1190 1200
AKEKAEKERL EKQQKREQLI DMNAEGDETG VMDSLLEALQ SGAAFRRKRG
1210 1220 1230 1240 1250
PRQVNRKAGC AVTSLLASEL TKDDAMAPGP VKVPKKSEGV PTILEEAKEL

VGRAS
Length:1,255
Mass (Da):139,343
Last modified:July 1, 1997 - v1
Checksum:i09404164873CA7C1
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PV41E9PV41_MOUSE
Protein diaphanous homolog 1
Diaph1
1,264Annotation score:
F6XC54F6XC54_MOUSE
Protein diaphanous homolog 1
Diaph1
1,220Annotation score:
D3Z074D3Z074_MOUSE
Protein diaphanous homolog 1
Diaph1
1,220Annotation score:
E9PXV7E9PXV7_MOUSE
Protein diaphanous homolog 1
Diaph1
1,255Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96963 mRNA Translation: AAC53280.1
CCDSiCCDS57121.1
PIRiT31065
RefSeqiNP_031884.1, NM_007858.4
UniGeneiMm.195916

Genome annotation databases

EnsembliENSMUST00000115634; ENSMUSP00000111297; ENSMUSG00000024456
GeneIDi13367
KEGGimmu:13367
UCSCiuc033hgk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96963 mRNA Translation: AAC53280.1
CCDSiCCDS57121.1
PIRiT31065
RefSeqiNP_031884.1, NM_007858.4
UniGeneiMm.195916

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V9DX-ray2.60A/B/C/D826-1163[»]
1Z2CX-ray3.00B/D69-451[»]
2BAPX-ray3.30A/B135-451[»]
C/D1145-1200[»]
2BNXX-ray2.40A/B131-516[»]
2F31X-ray2.10A135-367[»]
B1177-1196[»]
2V8FX-ray1.10C635-655[»]
3EG5X-ray2.70B/D69-451[»]
3O4XX-ray3.20A/B/C/D131-458[»]
E/F/G/H736-1200[»]
3OBVX-ray2.75A/B/C/D131-457[»]
E/F/G/H753-1209[»]
4UWXX-ray1.65A/B135-369[»]
ProteinModelPortaliO08808
SMRiO08808
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199221, 7 interactors
CORUMiO08808
DIPiDIP-29028N
IntActiO08808, 17 interactors
MINTiO08808
STRINGi10090.ENSMUSP00000111297

PTM databases

iPTMnetiO08808
PhosphoSitePlusiO08808

Proteomic databases

EPDiO08808
MaxQBiO08808
PaxDbiO08808
PRIDEiO08808

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115634; ENSMUSP00000111297; ENSMUSG00000024456
GeneIDi13367
KEGGimmu:13367
UCSCiuc033hgk.1 mouse

Organism-specific databases

CTDi1729
MGIiMGI:1194490 Diaph1

Phylogenomic databases

eggNOGiKOG1924 Eukaryota
ENOG410Y29H LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000293231
HOVERGENiHBG051357
InParanoidiO08808
KOiK05740
PhylomeDBiO08808

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6785631 ERBB2 Regulates Cell Motility
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiDiaph1 mouse
EvolutionaryTraceiO08808
PROiPR:O08808
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024456 Expressed in 282 organ(s), highest expression level in conjunctival fornix
CleanExiMM_DIAP1
ExpressionAtlasiO08808 baseline and differential
GenevisibleiO08808 MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR014767 DAD_dom
IPR010465 Drf_DAD
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR027653 Formin_Diaph1
IPR009408 Formin_homology_1
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF17 PTHR23213:SF17, 2 hits
PfamiView protein in Pfam
PF06345 Drf_DAD, 1 hit
PF06346 Drf_FH1, 1 hit
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDIAP1_MOUSE
AccessioniPrimary (citable) accession number: O08808
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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